[Histonet] Frozen section artefact
Hello, I have a puzzling artefact that I can't seem to correct in 7 micron frozen sections of rat and mouse Small Intestine. When completing an IHC run, parts of the section look great and the staining has worked fine - on other parts of the same section, the morphology is ruined by the appearance of several spindly striations or smears that run between individual villi and in some cases actually cover the majority of the section. Where this occurs the top half of the villi do not take up either the antibody staining or the Haematoxylin counterstain (which is taken up fine elsewhere). Sometimes the smearing looks so bad that it turns the section into a smeary, gloopy mess. From what I can see the origin of the smears appears to be the nuclei as I have seen several small spindles from the nuclei leading into a larger thread. We have thought about Lysis, Gut Mucus (have stained PAS to highlight), nuclear degredation. Could it be fixation? We air dry following sectioning and fix in Acetone / alcohol 3:1 for 5 mins. Any ideas would be great! Thanks in advance. Adam Adam Boanas Senior Research Associate Epistem Ltd 48 Grafton Street Manchester, M13 9XX This message has been scanned for malware by Websense. www.websense.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PAN CKMNF116, picking up dendritic cells?
It should label dendritic cells since it reacts with cytokeratin 8. These cells have been shown to contain cytokeratin protein by immunoblotting. I use this reactivity as an internal positive control. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-2204 Fax jAswigueAlatan msjaswigue.ala...@gmail.com 9/24/2012 9:14 PM Dear Histonetters, Has anyone out there experienced problem with optimizing PanCK MNF116 antibody? Our CKMNF116 stains dendritic cells on lymph nodes using Ventana protocol: CC1 - 8 mins, Ab incubation time 16 mins, Post primary peroxidase inhib selected. Basically, it should stain cytokeratins, but it stains dendritic cells as well. What would be the possible causes and solutions. Any suggestions would be greatly appreciated. Many thanks. Joy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] thermo tissue processor
Hi, I was hoping to get some input on the Thermo Scientific STP420ES Tissue Processor (the one that looks like a washing machine). Is anyone using it? Pros/Cons? Mandy Bell HTL(ASCP) Community Hospital of the Monterey Peninsula 2 Harris Court Suite B3/B4 Monterey, CA 93940 (831) 647-4791 Confidentiality Notice: This is a transmission from Community Hospital of the Monterey Peninsula. This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes. They are intended only for the use of the addressee. If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited. If you received this transmission in error, please accept our apologies and notify the sender. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Rat Brain Serial Sections
Hi Histonet- I have to serial section rat brain slices through the entire block (5 blocks total per brain) for stereology. Since it is for stereology, I obviously need to minimize tissue loss while sectioning. Does anyone have any experience with using ammonium hydroxide to help with the dryness? Would you recommend soaking the block in ammonium hydroxide or wiping the surface of the block with it between sections? Also, what dilution would you use? Thanks in advance for your help! Erin Sarricks, HT (ASCP) Histology Team Leader USAMRICD Comparative Pathology Branch ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Annual NSH-IHC Resource Group Meeting
Greetings All: For those of you who are planning to attend the National Society for Histotechnology's Symposium/Convention later this week, I just wanted to remind everyone that our group will hold it's only 'annual meeting' on Saturday, 9/29, from 12:00 to 12:45, (somewhere) in the Convention Center. If you'd like to attend, please stop by the IHC-RG's table, located in the Exhibit Hall, and pick up a copy of the tentative agenda. For those of you who do not plan on attending the S/C (as well as those who do), I will post a summary report on the IHC-RG's GoogleGroup 'listerv' after the meeting has concluded. I look forward to seeing many of you in Vancouver! Best Wishes, Joe Myers Chair, IHC-RG ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Albumin and Epcam to stain mouse cell pellets
Good afternoon, Has anyone used Albumin and Epcam antibodies to stain mouse samples? If you have would you please share the antibody information and conditions with me? Thank you, Eva Permaul Georgetown University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Frozen section artefact
Assuming that you are fixing fresh-frozen tissue sections: Tissue is autolysing. Use Formalin for 10 mins or 1:1 acetone:alcohol for 10 mins. Imho, acetone is not a fixative..it's a delipidizer. 5 mins in that mixture is too short a time for the alcohol to be an effective coagulant fixer. However, a longer time in alcohol may well mask your Ag sites. I see this artefact in acetone fixed frozen sections, often. When immunostaining for MHCs in muscle, no fixation is required but, if DAPI/Hoechst is includednuclear streaming will be seen. Always try several fixing fluids to get the best results on any new Ab. I may be wronghappy to be corrected. Curious always, Carl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] P16 and HPV
I've been told that the HPV testing will be discontinued as of end of year. HPV expresses the P16 gene but so does other type of cancers associated with HPV. It is possible to have a P16 pos and HPV negative test. But is it not possible to have HPV pos and P16 neg. if HPV is positive then the P16 is also positive. So the P16 cannot be used to replace the HPV testing can it? Antoinette Crill TEAM LEADER ANATOMIC PATHOLOGY ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Albumin and Epcam to stain mouse cell pellets
Eva For mouse EpCAM I have used routinely during past 15 years G8.8 rat monoclonal antibody from Developmental Studies Hybridoma Bank. Concentrated supernatant (1:200 dilution for IF) works very well on 4% paraformaldehyde fixed frozen samples (or on sections from fresh-frozen samples post-fixed with formaldehyde). Epitope is very sensitive to alcohol, so even fixation with NBF instead of formaldehyde diminishes staining dramatically. I was told that this antibody works on formalin-fixed paraffin sections after HIER retrieval, but never got results comparable to frozen sections. Don't have any recommendations regarding albumin, I used home-made antibodies for that purpose many years ago. In general, paraformaldehyde fixation and frozen sections worked satisfactory. Classical approach for albumin and alpha-fetoprotein staining was to fix in Saint-Mary fixative (1% acetic acid in absolute ethanol) following routine paraffin embedding. Nevertheless, short fixation with NBF (4h at room temp, following PBS wash) and paraffin embedding works as well. Best results regarding liver secreted proteins (as well as all membrane and cytoskeletal markers) could be achieved on cryo-sections after fixation by perfusion with 4% paraformaldehyde in PBS. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Tuesday, September 25, 2012 1:21 PM To: histonet Subject: [Histonet] Albumin and Epcam to stain mouse cell pellets Good afternoon, Has anyone used Albumin and Epcam antibodies to stain mouse samples? If you have would you please share the antibody information and conditions with me? Thank you, Eva Permaul Georgetown University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Albumin and Epcam to stain mouse cell pellets
Thank you Anatoli. Let me add some more info to my question. The samples are FFPE. Thanks, Eva On Tue, Sep 25, 2012 at 2:43 PM, Anatoli Gleiberman agleiber...@cbiolabs.com wrote: Eva For mouse EpCAM I have used routinely during past 15 years G8.8 rat monoclonal antibody from Developmental Studies Hybridoma Bank. Concentrated supernatant (1:200 dilution for IF) works very well on 4% paraformaldehyde fixed frozen samples (or on sections from fresh-frozen samples post-fixed with formaldehyde). Epitope is very sensitive to alcohol, so even fixation with NBF instead of formaldehyde diminishes staining dramatically. I was told that this antibody works on formalin-fixed paraffin sections after HIER retrieval, but never got results comparable to frozen sections. Don't have any recommendations regarding albumin, I used home-made antibodies for that purpose many years ago. In general, paraformaldehyde fixation and frozen sections worked satisfactory. Classical approach for albumin and alpha-fetoprotein staining was to fix in Saint-Mary fixative (1% acetic acid in absolute ethanol) following routine paraffin embedding. Nevertheless, short fixation with NBF (4h at room temp, following PBS wash) and paraffin embedding works as well. Best results regarding liver secreted proteins (as well as all membrane and cytoskeletal markers) could be achieved on cryo-sections after fixation by perfusion with 4% paraformaldehyde in PBS. Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eva Permaul Sent: Tuesday, September 25, 2012 1:21 PM To: histonet Subject: [Histonet] Albumin and Epcam to stain mouse cell pellets Good afternoon, Has anyone used Albumin and Epcam antibodies to stain mouse samples? If you have would you please share the antibody information and conditions with me? Thank you, Eva Permaul Georgetown University ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit
We are trying to decide how to validate our stains when we switch from Ventana's IView kit to their Ultraview Kit. I have reviewed the CAP question on this and find the following wording: The performance of new lots of antibody and detection system reagents are compared with old lots before or concurrently with being placed into service. Note: Parallel staining is required to control for variables such as disparity in the lots of detection reagents or instrument function. New lots of primary and detection reagents must be compared to the previous lot using an appropriate panel of control tissues. This comparison must be made on slides cut from the same control block. Evidence: Written procedure and records of verification of new reagent lots. For new lots of antibodies we have been running the new lot and comparing with the previous lot by reviewing the control slide from the old lot to the new lot. Is this sufficient? Wording that bothers me is appropriate panel of tissues Thanks for your input. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit
As far as lot to lot validation that's all we do. Use same control and compare both. Now validating a new detection kit is a whole different story. Here I just made a checklist of all the antibodies we do and had the doc sign off on each stain with the new kit. If you want you can do a slide of each with same control one with the iview and one with the ultraview. All depends on how your doc wants to validate it. Vanessa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, September 25, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit We are trying to decide how to validate our stains when we switch from Ventana's IView kit to their Ultraview Kit. I have reviewed the CAP question on this and find the following wording: The performance of new lots of antibody and detection system reagents are compared with old lots before or concurrently with being placed into service. Note: Parallel staining is required to control for variables such as disparity in the lots of detection reagents or instrument function. New lots of primary and detection reagents must be compared to the previous lot using an appropriate panel of control tissues. This comparison must be made on slides cut from the same control block. Evidence: Written procedure and records of verification of new reagent lots. For new lots of antibodies we have been running the new lot and comparing with the previous lot by reviewing the control slide from the old lot to the new lot. Is this sufficient? Wording that bothers me is appropriate panel of tissues Thanks for your input. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Path reports
Good afternoon Histoland! Anyone that is using Meditech AP module 5.6 please grace me with your method of transcription/dictation for your Pathologist. We just went live this year and it seems we have stepped back in time with this system. The voice recognition system we use in Radiology is not combatible with Meditech..ughhh! Thanks in advance for any help or direction! Michelle Moore Schneider Regional Medical Center 9048 Sugar Estate St. Thomas Virgin Islands 00802 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Xylene Free Process
Hello, I have been processing our tissue (derm only) in our Shandon Excelsior using a xylene free process with Iso Alcohol. A few years back it was set up that way by a Thermo rep and it worked really well. I am now having issues and the docs seem to think it is a processing issue rather than the sectioning or staining. I was wondering if anyone else is using a similar process and if you would mind sharing your overnight program with me for comparison . Thank you in advance for any help! V.Avalos ADS Fax:602-277-2134 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit
We are having a lively discussion about having 10 known positives and 10 known negatives to validate new antibodies. Many years ago we set up 5 and 5 even before CAP thought of the idea. This year's checklist added the 10 and 10 part, but it is up to the medical director. What is everyone else doing out there? We are using the Ventana UltraView detection kits. Everyone who uses these kits know how expensive they are. Is 5 and 5 sufficient or should go by CAP recommendations? Joe Nocito -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Tuesday, September 25, 2012 2:37 PM To: Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit As far as lot to lot validation that's all we do. Use same control and compare both. Now validating a new detection kit is a whole different story. Here I just made a checklist of all the antibodies we do and had the doc sign off on each stain with the new kit. If you want you can do a slide of each with same control one with the iview and one with the ultraview. All depends on how your doc wants to validate it. Vanessa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, September 25, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit We are trying to decide how to validate our stains when we switch from Ventana's IView kit to their Ultraview Kit. I have reviewed the CAP question on this and find the following wording: The performance of new lots of antibody and detection system reagents are compared with old lots before or concurrently with being placed into service. Note: Parallel staining is required to control for variables such as disparity in the lots of detection reagents or instrument function. New lots of primary and detection reagents must be compared to the previous lot using an appropriate panel of control tissues. This comparison must be made on slides cut from the same control block. Evidence: Written procedure and records of verification of new reagent lots. For new lots of antibodies we have been running the new lot and comparing with the previous lot by reviewing the control slide from the old lot to the new lot. Is this sufficient? Wording that bothers me is appropriate panel of tissues Thanks for your input. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] NSH Convention Symposium - Quality Control Committe Meeting
The NSH is upon us and time to meet, greet and exchange. If you are attending and want to become involved in discussing and supporting Quality Improvement in the HIstology Lab, then attend the Quality Control Committee meeting on Saturday morning, September 29, 2012, 7:00 am - 7:45 am or stop by the committee table during exhibit hours. The committee will discuss:2012 Quality Management Forum - Creating the Quality Chain in Histology, Bethesda, MD, October 20, 20122013 Forum - format and speakersAsk NSH About Quality - process and supportQuality Management in Histology Project - sub-committee and contentTissue Control Bank Bring yourself and your ideas. Hope to see you at NSH and at the committee meeting. William DeSalvo, B.S., HTL(ASCP) Chair, NSH Quality Control Committee ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Have Blocks, Will Cut
Hello, Histonetters, If you are in the South (specifically TN, AL, AR, MS, GA, KY),and represent or know of a laboratory that may be short or inadeqautely staffed, I can help. I care about timely patient care and helping to produce diagnosis as timely as possible. If a need exists for a high volume of slides to be produced in a short amount of time, I can help and am available to assist on your busiest days to keep stress and fatigue to a minimum on your regular employees. Embedding with correct orientation-- 120+blocks/per hour Microtomy fully-faced block with precision and wrinkle-free sections-- 90-100+/per hour Confidentiality is important and your reasons for needing assistance will not be shared or disclosed. Photos of work can be viewed at www.HistoCare.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit
Joe I believe that is for initial validation, you can also place multiple tissues on a slide. For new lot numbers of a previously validated protocol I would use only 3 tissues, strong positive, moderate to weak positive and then negative, these can all be placed on one slide. I'm not in clinical but that's also the recommendations from the CAP paper on standardization in IHC. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Tuesday, September 25, 2012 4:35 PM To: 'Vanessa Perez'; 'Vickroy, Jim'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit We are having a lively discussion about having 10 known positives and 10 known negatives to validate new antibodies. Many years ago we set up 5 and 5 even before CAP thought of the idea. This year's checklist added the 10 and 10 part, but it is up to the medical director. What is everyone else doing out there? We are using the Ventana UltraView detection kits. Everyone who uses these kits know how expensive they are. Is 5 and 5 sufficient or should go by CAP recommendations? Joe Nocito -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vanessa Perez Sent: Tuesday, September 25, 2012 2:37 PM To: Vickroy, Jim; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit As far as lot to lot validation that's all we do. Use same control and compare both. Now validating a new detection kit is a whole different story. Here I just made a checklist of all the antibodies we do and had the doc sign off on each stain with the new kit. If you want you can do a slide of each with same control one with the iview and one with the ultraview. All depends on how your doc wants to validate it. Vanessa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, September 25, 2012 1:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Changing from Ventana IView Detection Kit to Ventana Ultraview kit We are trying to decide how to validate our stains when we switch from Ventana's IView kit to their Ultraview Kit. I have reviewed the CAP question on this and find the following wording: The performance of new lots of antibody and detection system reagents are compared with old lots before or concurrently with being placed into service. Note: Parallel staining is required to control for variables such as disparity in the lots of detection reagents or instrument function. New lots of primary and detection reagents must be compared to the previous lot using an appropriate panel of control tissues. This comparison must be made on slides cut from the same control block. Evidence: Written procedure and records of verification of new reagent lots. For new lots of antibodies we have been running the new lot and comparing with the previous lot by reviewing the control slide from the old lot to the new lot. Is this sufficient? Wording that bothers me is appropriate panel of tissues Thanks for your input. James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
