Re: [Histonet] Metal molds
I clean them about every 10 days or so. I run them through the cleaning cycle on my VIP 5. O'Donnell, Bill billodonn...@catholichealth.net 10/8/2012 4:31 PM OK folks, I know I should be smarter than this and I haven't seen discussion on it lately Are people cleaning their metal embedding molds after evey embedding session? If not, how often do you clean them? Do you clean them at all? If you clean them, how do you do it? Thanks Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Metal molds
We don't clean them after each use, but we do put them in the oven (in a filter over a container) so the wax melts. Every so often, not sure of the frequency, we clean in CitriSolv (our solvent instead of Xylene). Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Tuesday, October 09, 2012 8:34 AM To: Bill O'Donnell; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Metal molds I clean them about every 10 days or so. I run them through the cleaning cycle on my VIP 5. O'Donnell, Bill billodonn...@catholichealth.net 10/8/2012 4:31 PM OK folks, I know I should be smarter than this and I haven't seen discussion on it lately Are people cleaning their metal embedding molds after evey embedding session? If not, how often do you clean them? Do you clean them at all? If you clean them, how do you do it? Thanks Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: RE: Specimens for TEM
Just one minor change in the below mentioned protocol. Do you really do water washes? I have never heard of that. We do buffer rinses. (I personally used Cacodylate buffer, but people also use Phosphate buffer). Peggy Sherwood Research Specialist, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Duraine, Lita Sent: Friday, October 05, 2012 1:22 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Specimens for TEM Hi Allyse, TEM is a whole new ball of wax so to speak. It depends on what you are fixing, and how you plan to fix it. Typically you would start with Paraformaldehyde and a Glutaraldehyde mix such as Karnovsky's. The after water washes do a secondary fix in Osmium Tetroxide followed by water washes. Next would be dehydration with graded ethanol series. Final dehydration in Propylene Oxide (PO) then into PO and Resin mixes until finally into pure resin. I am not sure about 7100 plastic resin, but we use Embed 812 with NMA, DDSA, and DMP-30 as the hardener. This resin is very common in EM as is very stable under the electron beam. You can also use LR White resin or another product called Spurrs. It depends on what you are trying to accomplish and weather it will be labeled or not. If you need a recipe I can send it to you. The tissue is the question. Lung and other tissue pose infiltration problems and you might want to look into microwave and vacuum processing. We routinely use microwave and vacuum processing for all our tissues. I find it cuts down on time from three days to six hours. If you are not set-up for EM processing then you can send it out. First they should ask you what you want to study in your EM images and then they will tell you how to prepare the tissue to send to them. The types of organelles and what you want to see in your images will determine how you fix your tissue. Just as in Histology there are layers of knowledge for the right situation. It is the same for EM. Good luck, Lita Duraine EM Technologist Bellen Lab HHMI- Molecular Genetics Duncan Neurological Research Institute 1250 Moursund St. Houston, TX 77030 Rm: N1165.17 MS: N1125.50 832-824-8772 TEM Room 979-549-6526 Cell http://flypush.imgen.bcm.tmc.edu/lab/people/lita.php ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Problem in peloris processor
Dear All! We processed the 3mm biopsies in peloris processor using factory 4 hrs. xylene standard protocol. But the processing was poor (soft) and almost half of the biopsies didn't process well reagent status was ok. Advised require regarding this problem. Regards. Muhammad Tahseen Pathology (Histology) SKMCHRC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Metal molds
Yes, after each embedding session. It is very simple: you just place your molds in the tissue processor in the cleaning cycle and they will come out clean and sparkling. René J. From: O'Donnell, Bill billodonn...@catholichealth.net To: histonet@lists.utsouthwestern.edu Sent: Monday, October 8, 2012 4:31 PM Subject: [Histonet] Metal molds OK folks, I know I should be smarter than this and I haven't seen discussion on it lately Are people cleaning their metal embedding molds after evey embedding session? If not, how often do you clean them? Do you clean them at all? If you clean them, how do you do it? Thanks Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Problem in peloris processor
How long are your specimens fixed for before processing? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of tahs...@brain.net.pk Sent: Tuesday, October 09, 2012 10:17 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Problem in peloris processor Dear All! We processed the 3mm biopsies in peloris processor using factory 4 hrs. xylene standard protocol. But the processing was poor (soft) and almost half of the biopsies didn't process well reagent status was ok. Advised require regarding this problem. Regards. Muhammad Tahseen Pathology (Histology) SKMCHRC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mitochondiral membrane
Dear all- I have a user looking to label mitochondrial membrane. Can anybody recommend a commercial antibody? I'm guessing one of the TOM complex proteins would be a good target. Thank you in advance. Sho Fujisawa Molecular Cytology Core Facility Memorial Sloan-Kettering Cancer Center 415 East 68th St. ZRC-1840 New York, NY 10065 (646) 888-2186, x2186 = Please note that this e-mail and any files transmitted from Memorial Sloan-Kettering Cancer Center may be privileged, confidential, and protected from disclosure under applicable law. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this communication or any of its attachments is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting this message, any attachments, and all copies and backups from your computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] Metal molds
We clean them every day. Ultrasonic bath with soap-water (dishwasher) at 40-50 degrees, 15 min. Paraffin swims on the surface. Molds are put out and air dried. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von O'Donnell, Bill Gesendet: Montag, 08. Oktober 2012 22:32 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Metal molds OK folks, I know I should be smarter than this and I haven't seen discussion on it lately Are people cleaning their metal embedding molds after evey embedding session? If not, how often do you clean them? Do you clean them at all? If you clean them, how do you do it? Thanks Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Metal molds
We clean our molds once a week. Soak them in Xylene to remove paraffin, soak in 100% alcohol to remove xylene, rinse in running water, dry and spray with mold release solution. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, October 08, 2012 4:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Metal molds OK folks, I know I should be smarter than this and I haven't seen discussion on it lately Are people cleaning their metal embedding molds after evey embedding session? If not, how often do you clean them? Do you clean them at all? If you clean them, how do you do it? Thanks Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet =This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Mitochondiral membrane
Sho There is a mitochondria marker for human tissue samples they are from Novus, not sure if it's a membrane marker or not. Good Luck! Protocol: Mouse anti Mitochondria Clone: MTC02 Vendor: Novus Biologicals Catalog Number: NB600-556 Species: Mouse Isotype: IgG21 Spec Sheet: Novus Mitohttp://www.novusbio.com/data_sheet/index/NB600-556prev_module=search Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of fujis...@mskcc.org Sent: Tuesday, October 09, 2012 8:43 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Mitochondiral membrane Dear all- I have a user looking to label mitochondrial membrane. Can anybody recommend a commercial antibody? I'm guessing one of the TOM complex proteins would be a good target. Thank you in advance. Sho Fujisawa Molecular Cytology Core Facility Memorial Sloan-Kettering Cancer Center 415 East 68th St. ZRC-1840 New York, NY 10065 (646) 888-2186, x2186 = Please note that this e-mail and any files transmitted from Memorial Sloan-Kettering Cancer Center may be privileged, confidential, and protected from disclosure under applicable law. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this communication or any of its attachments is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting this message, any attachments, and all copies and backups from your computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cellient
I am curious if there are any instituions that are using the Cellient instrument for cell blocks on ECC cases that are scant or any surgical specimen. -- Kevin Schofield BS CT(ASCP) Director, Outreach Clinical Operations Yale University Department of Pathology (P) 203-737-2928 (F) 203-785-2641 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Flourescent mounting medium- PLEASE HELP!!!
We use this medium. We have found that if you shake it before use it becomes a little more viscous, but toss it if it becomes too gel like. We also allow our sections to dry, but keep them in the dark as much as possible. You also need to be sure that you are using coverglass that works with fluorescent material. Laura -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jonathan Cremer Sent: Monday, October 08, 2012 7:20 AM To: Candice Smoots; Histonet Subject: RE: [Histonet] Flourescent mounting medium- PLEASE HELP!!! I have no experience with this particular mounting medium, but from what I gather it is like any other aqueous mounting medium. You should blot your slides to remove as much water as possible, without letting your sections dry. Then apply a small amount of mounting medium to the slide or coverslip (depending on preference) and mount. Apparently it solidifies at the edge, so no need to seal with nail polish. If your bottle has solidified to the point where it is a gel instead of a viscous liquid, you can toss it. (unless the datasheet specifies otherwise...) Jonathan Van: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] namens Candice Smoots [candice_cami...@yahoo.com] Verzonden: maandag 8 oktober 2012 15:42 To: Histonet Onderwerp: [Histonet] Flourescent mounting medium- PLEASE HELP!!! Hi Histonet In our lab, we are trying to mount our flourescent stained sections with polyvinyl alcohol mounting medium with DABCO. WE are unsure if it is to be mounted while the sections are wet or do we let me air dry. Another question is... How to actually use it. It looks to be a little solidified. Kind of like jello. Is this normal? do I dilute it? Please Please Help! I remain yours truely, Candice Camille ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Metal molds
We haven't cleaned our molds for five years - if the blocks are cold enough after embedding, there is no problem removing the block. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, October 08, 2012 2:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Metal molds OK folks, I know I should be smarter than this and I haven't seen discussion on it lately Are people cleaning their metal embedding molds after evey embedding session? If not, how often do you clean them? Do you clean them at all? If you clean them, how do you do it? Thanks Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Reagent Lot Verification
Does anyone have a Lot to Lot Verification QC form or procedure that they would be willing to share with me for use with the Ventana Benchmark when comparing lot to lots of antibodies and detection kits? Thanks in advance for your help, Amy Georgetown Hospital System 843-527-7179 NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] prostate trimming protocol
Good morning all, Could someone with more knowledge in this matter than I have help shed a little light? While at a nationally-renowned medical facility, I've come across something rather interesting (to me) for which no one in the immediate lab has a definitive answer for. I see varying trimming(or grossing) techniques by the residents. I'm told that it's very common to have poorly grossed tissue submitted regularly whenever a new group comes through, but nothing is done to correct it. It runs the gamut from non-decalcified bone, or humongous chunks of tissue that barely fits in the cassette but has to be nearly shoved into the mold, and tissue that's 5mm, seriously. This time, it's prostate tissue. I've been places where maybe 3 or 4 sections were submitted from the area of interest and maybe a sample of normal tissue just for differentiation. But here, it's common to receive anywhere from 30 to 50 cassettes from the same site. I'm guessing they don't want to discard ANY tissue. What's interesting is some of this is submitted as a bunch of very tiny slivers in some cassettes and then nickel and quarter-sized chunks from the same site in others. Has anyone else seen prostate submitted this way? Is there a rhyme or reason that I'm not aware of? Thanks M www.HistoCare.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Mitochondiral membrane
I have use a mitochondria marker from Dako but it is cytoplasmic not on the membrane. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pru...@ihctech.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, October 09, 2012 8:55 AM To: 'fujis...@mskcc.org'; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Mitochondiral membrane Sho There is a mitochondria marker for human tissue samples they are from Novus, not sure if it's a membrane marker or not. Good Luck! Protocol: Mouse anti Mitochondria Clone: MTC02 Vendor: Novus Biologicals Catalog Number: NB600-556 Species: Mouse Isotype: IgG21 Spec Sheet: Novus Mitohttp://www.novusbio.com/data_sheet/index/NB600-556prev_module=search Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308-1592 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell www.premierlab.com Ship to address: 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of fujis...@mskcc.org Sent: Tuesday, October 09, 2012 8:43 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Mitochondiral membrane Dear all- I have a user looking to label mitochondrial membrane. Can anybody recommend a commercial antibody? I'm guessing one of the TOM complex proteins would be a good target. Thank you in advance. Sho Fujisawa Molecular Cytology Core Facility Memorial Sloan-Kettering Cancer Center 415 East 68th St. ZRC-1840 New York, NY 10065 (646) 888-2186, x2186 = Please note that this e-mail and any files transmitted from Memorial Sloan-Kettering Cancer Center may be privileged, confidential, and protected from disclosure under applicable law. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any reading, dissemination, distribution, copying, or other use of this communication or any of its attachments is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting this message, any attachments, and all copies and backups from your computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] prostate trimming protocol
Yes, and that depends, as you note, on the residents doing the grossing. Your solution: get the pathologists involved and find out how they want the grossing to be done, and make sure that the pathologists communicate their preferences to the residents. It may be that the ones wanting to see everything are the pathologists. Involve the pathologists in your concerns. René J. From: Contact HistoCare cont...@histocare.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, October 9, 2012 11:53 AM Subject: [Histonet] prostate trimming protocol Good morning all, Could someone with more knowledge in this matter than I have help shed a little light? While at a nationally-renowned medical facility, I've come across something rather interesting (to me) for which no one in the immediate lab has a definitive answer for. I see varying trimming(or grossing) techniques by the residents. I'm told that it's very common to have poorly grossed tissue submitted regularly whenever a new group comes through, but nothing is done to correct it. It runs the gamut from non-decalcified bone, or humongous chunks of tissue that barely fits in the cassette but has to be nearly shoved into the mold, and tissue that's 5mm, seriously. This time, it's prostate tissue. I've been places where maybe 3 or 4 sections were submitted from the area of interest and maybe a sample of normal tissue just for differentiation. But here, it's common to receive anywhere from 30 to 50 cassettes from the same site. I'm guessing they don't want to discard ANY tissue. What's interesting is some of this is submitted as a bunch of very tiny slivers in some cassettes and then nickel and quarter-sized chunks from the same site in others. Has anyone else seen prostate submitted this way? Is there a rhyme or reason that I'm not aware of? Thanks M http://www.histocare.com/ ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] prostate trimming protocol
Talk w/ the pathologists or residents, have a conversation about what and why. The multiple samples could be due to teaching or cancer protocols. The number, size and selection area of samples is always the responsibility of the pathologists. I find it best to not wonder why, just ask. Typically the pathologist will be more than happy to discuss and explain their dissection and sampling protocol. The variation in thickness and sample size by residents can be corrected by the trainer, typically a PA or pathologist. There may be a need to rewrite or create an good standardized procedure that the trainner can use. Again, have a discussion about how consistency in sample size, crisp edges and consistent thickness, 3 mm, can improve quality. I suggest you start w/ a visual, a good old nickel. Consider super gluing one to every gross dissection board. Precision at the grossing board leads to increased quality in processing, microtomy and staining. I am firm believer that if you concentrate on quality, both in action and conversation, you will get more bang for your buck! Always remember, when you start asking the questions, be prepared to get involved w/ training and correcting the process. William DeSalvo, B.S., HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Control Committee Owner/Consultant, Collaborative Advantage Consulting From: cont...@histocare.com Date: Tue, 9 Oct 2012 10:53:37 -0500 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] prostate trimming protocol Good morning all, Could someone with more knowledge in this matter than I have help shed a little light? While at a nationally-renowned medical facility, I've come across something rather interesting (to me) for which no one in the immediate lab has a definitive answer for. I see varying trimming(or grossing) techniques by the residents. I'm told that it's very common to have poorly grossed tissue submitted regularly whenever a new group comes through, but nothing is done to correct it. It runs the gamut from non-decalcified bone, or humongous chunks of tissue that barely fits in the cassette but has to be nearly shoved into the mold, and tissue that's 5mm, seriously. This time, it's prostate tissue. I've been places where maybe 3 or 4 sections were submitted from the area of interest and maybe a sample of normal tissue just for differentiation. But here, it's common to receive anywhere from 30 to 50 cassettes from the same site. I'm guessing they don't want to discard ANY tissue. What's interesting is some of this is submitted as a bunch of very tiny slivers in some cassettes and then nickel and quarter-sized chunks from the same site in others. Has anyone else seen prostate submitted this way? Is there a rhyme or reason that I'm not aware of? Thanks M www.HistoCare.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Prostate
I have seen this phenomenon in several locations, not just with residents. The thinking, as it was explained to me was that the little slivers were to be embedded on edge like a cone biopsy to determine margins and that the entire protstatic margin was to be submitted to be sure of complete tumor excision. The variability in thickness I can only attribute to ignorance, indifference, or sheer bullheadedness. I have gone around and around with Pathologists, PAs and Residents on this for decades. Sheehan and Hropchek have a table (I believe it is figure 14) detailing tissue thickness for submission and the effect on fixation. Good Luck getting it across to them. Terrence Wellen HT(ASCP) Legacy Health Systems Portland, OR ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 107, Issue 13 Prostate grossing
From: histonet-boun...@lists.utsouthwestern.edu on behalf of histonet-requ...@lists.utsouthwestern.edu Sent: Tue 10/9/2012 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 107, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Cellient (Schofield, Kevin) 2. RE: Flourescent mounting medium- PLEASE HELP!!! (Entwistle, Laura) 3. RE: Metal molds (Goins, Tresa) 4. prostate trimming protocol (Contact HistoCare) 5. New Reagent Lot Verification (Amy Self) 6. Re: prostate trimming protocol (Rene J Buesa) 7. RE: RE: Mitochondiral membrane (Patsy Ruegg) 8. RE: prostate trimming protocol (WILLIAM DESALVO) -- Message: 1 Date: Tue, 9 Oct 2012 15:00:37 + From: Schofield, Kevin kevin.schofi...@yale.edu Subject: [Histonet] Cellient To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Message-ID: 1c71b9cd502b094fa755e48906457c8f0938f...@x10-mbx3.yu.yale.edu Content-Type: text/plain; charset=us-ascii I am curious if there are any instituions that are using the Cellient instrument for cell blocks on ECC cases that are scant or any surgical specimen. -- Kevin Schofield BS CT(ASCP) Director, Outreach Clinical Operations Yale University Department of Pathology (P) 203-737-2928 (F) 203-785-2641 -- Message: 2 Date: Tue, 9 Oct 2012 15:22:43 + From: Entwistle, Laura lentwis...@ucsd.edu Subject: RE: [Histonet] Flourescent mounting medium- PLEASE HELP!!! To: Jonathan Cremer jonathan.cre...@med.kuleuven.be, Candice Smoots candice_cami...@yahoo.com, Histonet histonet@lists.utsouthwestern.edu Message-ID: a65c72fcd532314c9aafcc11bf5df5dd20981...@xmail-mbx-bh1.ad.ucsd.edu Content-Type: text/plain; charset=us-ascii We use this medium. We have found that if you shake it before use it becomes a little more viscous, but toss it if it becomes too gel like. We also allow our sections to dry, but keep them in the dark as much as possible. You also need to be sure that you are using coverglass that works with fluorescent material. Laura -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jonathan Cremer Sent: Monday, October 08, 2012 7:20 AM To: Candice Smoots; Histonet Subject: RE: [Histonet] Flourescent mounting medium- PLEASE HELP!!! I have no experience with this particular mounting medium, but from what I gather it is like any other aqueous mounting medium. You should blot your slides to remove as much water as possible, without letting your sections dry. Then apply a small amount of mounting medium to the slide or coverslip (depending on preference) and mount. Apparently it solidifies at the edge, so no need to seal with nail polish. If your bottle has solidified to the point where it is a gel instead of a viscous liquid, you can toss it. (unless the datasheet specifies otherwise...) Jonathan Van: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] namens Candice Smoots [candice_cami...@yahoo.com] Verzonden: maandag 8 oktober 2012 15:42 To: Histonet Onderwerp: [Histonet] Flourescent mounting medium- PLEASE HELP!!! Hi Histonet In our lab, we are trying to mount our flourescent stained sections with polyvinyl alcohol mounting medium with DABCO. WE are unsure if it is to be mounted while the sections are wet or do we let me air dry. Another question is... How to actually use it. It looks to be a little solidified. Kind of like jello. Is this normal? do I dilute it? Please Please Help! I remain yours truely, Candice Camille ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 3 Date: Tue, 9 Oct 2012 15:49:13 + From: Goins, Tresa tgo...@mt.gov Subject: [Histonet] RE: Metal molds To: O'Donnell, Bill billodonn...@catholichealth.net, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID:
[Histonet] Automated histochemistry?
Hi all, Has anyone tried doing muscle enzyme histochemistry on an automated stainer? If so, I'd appreciate any information about your instrumentation and methods. Thanks! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Metal Molds
We occasionally use metal molds and we clean them after every use. We do this by putting them into the tissue processor with the dirty rack during the clean cycle. Joanne Clark, HT Histology Supervisor Pathology Consultants of New Mexico -- Message: 9 Date: Mon, 8 Oct 2012 14:31:45 -0600 From: O'Donnell, Bill billodonn...@catholichealth.net Subject: [Histonet] Metal molds To: histonet@lists.utsouthwestern.edu Message-ID: 4940df6d1c5fdf48931b6966aaef93957a3...@chimsx08.chi.catholichealth.net Content-Type: text/plain; charset=us-ascii OK folks, I know I should be smarter than this and I haven't seen discussion on it lately Are people cleaning their metal embedding molds after evey embedding session? If not, how often do you clean them? Do you clean them at all? If you clean them, how do you do it? Thanks Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Process Improvements
Fawn, Could you not use something like the number of recuts, reembeds, staining repeats for something like that? For each parameter u could list suggestions for improving those repeat test to lower the numbers from month to month. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 pru...@ihctech.net -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Saturday, October 06, 2012 2:36 AM To: 'Fawn Bomar' Cc: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] Process Improvements I would recommend data, that are available through lab-IT. It is a kind of frustrating and time-consuming, if you have to monitor daily a large number of figures. E.g. we have an incoming time and a ready of report time in our system, that tells us the mean-time of histology testing. Or for medical results, the system should have the possibility to monitor the single parameters (e.g. estrogen positivity). So one can create a report out of IT. Everything you put into the system, should give you a report. And you should choose parameters, that can really be altered by yourself, if you are not content with the result. Often we see a problem, but everyone says: there's nothing we can change... that's out of our control. Gudrun -Ursprüngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Fawn Bomar Gesendet: Freitag, 05. Oktober 2012 16:40 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Process Improvements Hi everyone, My new manager would like me to come up with a list of process/performance improvements in the pathology department that can be measured on a monthly/quarterly basis, such as they do for other departments in the hospital, i.e. the ER measures the number of patient falls with 5 per quarter being their goal. I need advice on what to measure for our histology/pathology/cytology departments. Does anyone have any suggestions? Thank you Fawn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Metal molds
I always cleaned them daily, either the very hot water, soapy water method, with water running over them in the sink with them on their sides so it passes over them, not upright so the water sits in them- then a rinse in alcohol and completely air dry. Or you can always do the clean cycle with the racks, running them through xylene, etc. They come out very clean this way- used an old processor that was a backup for this most of the time. But I always did them daily, but also wiped each one out with gauze if I used them twice in an embedding session ( for more than one specimen in that large batch). Also I like metal, I hate those plastic ones. If you keep the block face surface of the mold warm-hot, and flatten before it turns completely white the specimen is at the surface and you are able to see the edges easily without a lot of facing. I think this saves time cutting through paraffin, and saves blades. Plus if the specimen is not flat enough, you see it right away and know if you must re-embed to get a complete, representative section, rather than after you have cut some superficial parts of some edges away and not others, only to have to re-embed anyhow. The other problems I see are when people are afraid of big molds- please if you are only taking one section, use one large enough to leave a perimeter. Don't try to squeeze it into a medium mold, you are unlikely to need multiple sections on one slide and it is much easier to get flat and get a good section. Also please put enough paraffin on top, so that when it is cool the layer over the grooves in the cassette is not so thin that you can clearly see the depressions. That little bit of paraffin is much cheaper than tech time in re-embedding and fussing with a block longer than you should. Not so much a big issue for many specimens, but anything hard/ dense, such as bone, cervix, uterus, leeps, ( you get the idea) it is not anchored enough without a good dose of paraffin, causing more chatter when you section, and maybe chipping out more frequently, or even the whole bottom surface to lift off the cassette. I guess I have some pet peeves with this topic, so thanks for letting me get that out! Joelle Weaver MAOM, HTL (ASCP) QIHC From: valerie.han...@parrishmed.com To: billodonn...@catholichealth.net; histonet@lists.utsouthwestern.edu Date: Tue, 9 Oct 2012 10:51:01 -0400 CC: Subject: [Histonet] RE: Metal molds We clean our molds once a week. Soak them in Xylene to remove paraffin, soak in 100% alcohol to remove xylene, rinse in running water, dry and spray with mold release solution. Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief Parrish Medical Center 951 N. Washington Ave. Titusville, Florida 32976 Phone:(321) 268-6333 ext. 7506 Fax: (321) 268-6149 valerie.han...@parrishmed.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, October 08, 2012 4:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Metal molds OK folks, I know I should be smarter than this and I haven't seen discussion on it lately Are people cleaning their metal embedding molds after evey embedding session? If not, how often do you clean them? Do you clean them at all? If you clean them, how do you do it? Thanks Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet = This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___
RE: [Histonet] Automated histochemistry?
No sorry Tim, I have only ever do these methods manually. I'd be curious to hear if anyone has automated these. Joelle Weaver MAOM, HTL (ASCP) QIHC From: timothy.mor...@ucsfmedctr.org To: histonet@lists.utsouthwestern.edu Date: Tue, 9 Oct 2012 17:51:22 + Subject: [Histonet] Automated histochemistry? Hi all, Has anyone tried doing muscle enzyme histochemistry on an automated stainer? If so, I'd appreciate any information about your instrumentation and methods. Thanks! Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center 505 Parnassus Ave, Box 1656 Room S570 San Francisco, CA 94143 (415) 353-1266 (ph) (415) 514-3403 (fax) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mohs Histotech opening in Dover, DE
Position Title: MOHS Histotech Shift: Monday-Friday/Full Time. This is a permanent/long term position Position Summary Performs histology and cytology preparation procedures on patient specimens submitted for the diagnoses of disease. Maintains specimen identity and integrity; produces high quality stained slides using standardized procedures to meet the Physician/Pathologist¹s requirements for microscopic evaluation; and performs quality control and preventative maintenance according to established policy. Works independently, organizing work to meet established policy and deadlines. Interacts with patient populations from neonate to elderly; with patients of all types of illnesses. Duties Processes, embeds, cuts and stains quality frozen tissue specimens within established time limits. Works with the Mohs Dermatologist to resolve quality control, patient, or procedural problems. Requirements At least 3 years of experience Ability to work Full Time To apply email an updated resume to bran...@alliedsearchpartners.com -- Brannon Owens Recruitment Manager Allied Search Partners ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re molds
Topic: cleaning holds From: histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Monday, October 08, 2012 2:32 PM To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] Metal molds OK folks, I know I should be smarter than this and I haven't seen discussion on it lately Are people cleaning their metal embedding molds after evey embedding session? If not, how often do you clean them? Do you clean them at all? If you clean them, how do you do it? Thanks Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! We clean our holds by boiling them in a metal bowl in water with dishwashing liquid and occasional agitation on a hotplate in the lab. Let them come to boiling and stir a few times to release any wax, then allow the whole thing to cool. The bulk of the wax should form a skin on the top of the water which can be removed and thrown away in the bin. The holds can then be rinsed well in hot tap water to remove any residual wax and then dried in a drying oven. Steve Weston Lab Manager Centre of Research Excellence for Chronic Respiratory Disease. Menzies Research Institute 0408990859 62264871 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
