RE: [Histonet] RE: Metal molds

2012-10-10 Thread Susan.Walzer
We put our molds in the VIP before running the cleaning cycle daily. Then we 
dip them in alcohol containing mold release..air dry and store.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Tuesday, October 09, 2012 3:27 PM
To: valerie.han...@parrishmed.com
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds


I always cleaned them daily, either the very hot water, soapy water method, 
with water running over them in the sink with them on their sides so it passes 
over them, not upright so the water sits in them- then a rinse in alcohol and 
completely air dry. Or you can always do the clean cycle with the racks, 
running them through xylene, etc. They come out very clean this way- used an 
old processor that was a backup for this most of the time. But I always did 
them daily, but also wiped each one out with gauze if I used them twice in an 
embedding session ( for more than one specimen in that large batch). Also I 
like metal, I hate those plastic ones. If you keep the block face surface of 
the mold warm-hot,  and flatten before it turns completely white the specimen 
is at the surface and you are able to see the edges easily without a lot of 
facing. I think this saves time cutting through paraffin, and saves blades. 
Plus if the specimen is not flat enough, you see it right away and know if you 
must re-embed to get a complete, representative section, rather than after you 
have cut some superficial parts of some edges away and not others, only to have 
to re-embed anyhow. The other problems I see are when people are afraid of 
big molds- please if you are only taking one section, use one large enough to 
leave a perimeter. Don't try to squeeze it into a medium mold, you are unlikely 
to need multiple sections on one slide and it is much easier to get flat and 
get a good section.  Also please  put enough paraffin on top, so that when it 
is cool the layer over the grooves in the cassette is not so thin that you can 
clearly see the depressions. That little bit of paraffin is much cheaper than 
tech time in re-embedding and fussing with a block longer than you should.  Not 
so much a big issue for many specimens, but anything hard/ dense, such as bone, 
cervix, uterus, leeps, ( you get the idea) it is not anchored enough without a 
good dose of paraffin, causing more chatter when you section, and maybe 
chipping out more frequently, or even the whole bottom surface to lift off the 
cassette. I guess I have some pet peeves with this topic,  so thanks for 
letting me get that out!




Joelle Weaver MAOM, HTL (ASCP) QIHC
  From: valerie.han...@parrishmed.com
 To: billodonn...@catholichealth.net; histonet@lists.utsouthwestern.edu
 Date: Tue, 9 Oct 2012 10:51:01 -0400
 CC: 
 Subject: [Histonet] RE: Metal molds
 
 We clean our molds once a week. Soak them in Xylene to remove paraffin, soak 
 in 100% alcohol to remove xylene, rinse in running water, dry and spray with 
 mold release solution.
 
 Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
 Histology Section Chief
 Parrish Medical Center
 951 N. Washington Ave.
 Titusville, Florida 32976
 Phone:(321) 268-6333 ext. 7506
 Fax: (321) 268-6149
 valerie.han...@parrishmed.com
  
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, 
 Bill
 Sent: Monday, October 08, 2012 4:32 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Metal molds
 
 
  OK folks, I know I should be smarter than this and I haven't seen discussion 
 on it lately 
 
 Are people cleaning their metal embedding molds after evey embedding session?
 
 If not, how often do you clean them? 
 
 Do you clean them at all?
 
 If you clean them, how do you do it? 
 
 Thanks
 
 Bill
 William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan 
 Hospital 10 East 31st Street Kearney, NE 68847 
 
 SERENITY is not freedom from the storm, but peace amid the storm.
 
 Cultivate it in PRAYER!
 
  
 
 
 This electronic mail and any attached documents are intended solely for the 
 named addressee(s) and contain confidential information. If you are not an 
 addressee, or responsible for delivering this email to an addressee, you have 
 received this email in error and are notified that reading, copying, or 
 disclosing this email is prohibited. If you received this email in error, 
 immediately reply to the sender and delete the message completely from your 
 computer system.
 
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 This email is intended solely for the use of the individual to
 whom it is addressed and may contain information that is
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 under 

[Histonet] Dayshift temp or perm for experienced grossing HT

2012-10-10 Thread Cheryl
Good morning 'Netters-
 
We're seeking a experienced grossing Histotech for a group of small biopsy 
clinics.  Must have gross experience.  The pay is GREAT and the work 
environment if fast-paced and fun.  I've been working with the group for 
several years and they're growing.
 
We need to cover the position right away, thus the temp option.  If you can't 
temp but are interested in the job, please apply--we will consider all 
qualified applicants.
 
 
Due to the speed of response, please include a current resume with your 
response.  
NOTE: we will consider non-degreed candidates who are CLIA qualified to gross.  
If you aren't sure--call.  
 
The position is in Houston TX
 
 
Many thanks!
 
admin@fullstaff,org
281.852.9457
 
 
Cheryl

Cheryl Kerry, HT(ASCP) 
Full Staff Inc. 
Staffing the AP Lab by helping one GREAT Tech at a time.  
281.852.9457 Office
800.756.3309 Phone  Fax 
ad...@fullstaff.org 

Sign up for the FREE newsletter AP News--updates, tricks of the trade and 
current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request 
to apn...@fullstaff.org. Please include your name and specialty in the body of 
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RE: [Histonet] RE: Metal molds

2012-10-10 Thread Harris, Diana
At our lab we clean the molds in the VIP cleaning cycle then dip in mold 
release also.  Works well.  The only precaution is to drain the molds well 
after dipping otherwise embedding can be effected.


Diana Harris
QC  Method Development Technologist
Dept. Of Laboratory Medicine
Anatomical Pathology
Royal Jubilee Hospital
Victoria, BC Canada



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
susan.wal...@hcahealthcare.com
Sent: Wednesday, October 10, 2012 12:01 AM
To: joellewea...@hotmail.com; valerie.han...@parrishmed.com
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds

We put our molds in the VIP before running the cleaning cycle daily. Then we 
dip them in alcohol containing mold release..air dry and store.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Tuesday, October 09, 2012 3:27 PM
To: valerie.han...@parrishmed.com
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds


I always cleaned them daily, either the very hot water, soapy water method, 
with water running over them in the sink with them on their sides so it passes 
over them, not upright so the water sits in them- then a rinse in alcohol and 
completely air dry. Or you can always do the clean cycle with the racks, 
running them through xylene, etc. They come out very clean this way- used an 
old processor that was a backup for this most of the time. But I always did 
them daily, but also wiped each one out with gauze if I used them twice in an 
embedding session ( for more than one specimen in that large batch). Also I 
like metal, I hate those plastic ones. If you keep the block face surface of 
the mold warm-hot,  and flatten before it turns completely white the specimen 
is at the surface and you are able to see the edges easily without a lot of 
facing. I think this saves time cutting through paraffin, and saves blades. 
Plus if the specimen is not flat enough, you see it right away and know if you 
must re-embed to get a complete, representative section, rather than after you 
have cut some superficial parts of some edges away and not others, only to have 
to re-embed anyhow. The other problems I see are when people are afraid of 
big molds- please if you are only taking one section, use one large enough to 
leave a perimeter. Don't try to squeeze it into a medium mold, you are unlikely 
to need multiple sections on one slide and it is much easier to get flat and 
get a good section.  Also please  put enough paraffin on top, so that when it 
is cool the layer over the grooves in the cassette is not so thin that you can 
clearly see the depressions. That little bit of paraffin is much cheaper than 
tech time in re-embedding and fussing with a block longer than you should.  Not 
so much a big issue for many specimens, but anything hard/ dense, such as bone, 
cervix, uterus, leeps, ( you get the idea) it is not anchored enough without a 
good dose of paraffin, causing more chatter when you section, and maybe 
chipping out more frequently, or even the whole bottom surface to lift off the 
cassette. I guess I have some pet peeves with this topic,  so thanks for 
letting me get that out!




Joelle Weaver MAOM, HTL (ASCP) QIHC
  From: valerie.han...@parrishmed.com
 To: billodonn...@catholichealth.net; histonet@lists.utsouthwestern.edu
 Date: Tue, 9 Oct 2012 10:51:01 -0400
 CC: 
 Subject: [Histonet] RE: Metal molds
 
 We clean our molds once a week. Soak them in Xylene to remove paraffin, soak 
 in 100% alcohol to remove xylene, rinse in running water, dry and spray with 
 mold release solution.
 
 Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
 Histology Section Chief
 Parrish Medical Center
 951 N. Washington Ave.
 Titusville, Florida 32976
 Phone:(321) 268-6333 ext. 7506
 Fax: (321) 268-6149
 valerie.han...@parrishmed.com
  
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, 
 Bill
 Sent: Monday, October 08, 2012 4:32 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Metal molds
 
 
  OK folks, I know I should be smarter than this and I haven't seen discussion 
 on it lately 
 
 Are people cleaning their metal embedding molds after evey embedding session?
 
 If not, how often do you clean them? 
 
 Do you clean them at all?
 
 If you clean them, how do you do it? 
 
 Thanks
 
 Bill
 William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan 
 Hospital 10 East 31st Street Kearney, NE 68847 
 
 SERENITY is not freedom from the storm, but peace amid the storm.
 
 Cultivate it in PRAYER!
 
  
 
 
 This electronic mail and any attached documents are intended solely for the 
 named addressee(s) and contain confidential information. If you are not an 
 

RE: [Histonet] RE: Metal molds

2012-10-10 Thread Bartlett, Jeanine (CDC/OID/NCEZID)
Does anyone that uses the VIP to clean their base molds also recycle the 
alcohols/xylene?  We were told that you should not clean the molds in the 
processor if you were recycling. Thanks!

Jeanine H. Bartlett
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
404-639-3590
jeanine.bartl...@cdc.hhs.gov

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harris, Diana
Sent: Wednesday, October 10, 2012 9:42 AM
To: 'susan.wal...@hcahealthcare.com'; joellewea...@hotmail.com; 
valerie.han...@parrishmed.com
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds

At our lab we clean the molds in the VIP cleaning cycle then dip in mold 
release also.  Works well.  The only precaution is to drain the molds well 
after dipping otherwise embedding can be effected.


Diana Harris
QC  Method Development Technologist
Dept. Of Laboratory Medicine
Anatomical Pathology
Royal Jubilee Hospital
Victoria, BC Canada



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
susan.wal...@hcahealthcare.com
Sent: Wednesday, October 10, 2012 12:01 AM
To: joellewea...@hotmail.com; valerie.han...@parrishmed.com
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds

We put our molds in the VIP before running the cleaning cycle daily. Then we 
dip them in alcohol containing mold release..air dry and store.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Tuesday, October 09, 2012 3:27 PM
To: valerie.han...@parrishmed.com
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds


I always cleaned them daily, either the very hot water, soapy water method, 
with water running over them in the sink with them on their sides so it passes 
over them, not upright so the water sits in them- then a rinse in alcohol and 
completely air dry. Or you can always do the clean cycle with the racks, 
running them through xylene, etc. They come out very clean this way- used an 
old processor that was a backup for this most of the time. But I always did 
them daily, but also wiped each one out with gauze if I used them twice in an 
embedding session ( for more than one specimen in that large batch). Also I 
like metal, I hate those plastic ones. If you keep the block face surface of 
the mold warm-hot,  and flatten before it turns completely white the specimen 
is at the surface and you are able to see the edges easily without a lot of 
facing. I think this saves time cutting through paraffin, and saves blades. 
Plus if the specimen is not flat enough, you see it right away and know if you 
must re-embed to get a complete, representative section, rather than after you 
have cut some superficial parts of some edges away and not others, only to have 
to re-embed anyhow. The other problems I see are when people are afraid of 
big molds- please if you are only taking one section, use one large enough to 
leave a perimeter. Don't try to squeeze it into a medium mold, you are unlikely 
to need multiple sections on one slide and it is much easier to get flat and 
get a good section.  Also please  put enough paraffin on top, so that when it 
is cool the layer over the grooves in the cassette is not so thin that you can 
clearly see the depressions. That little bit of paraffin is much cheaper than 
tech time in re-embedding and fussing with a block longer than you should.  Not 
so much a big issue for many specimens, but anything hard/ dense, such as bone, 
cervix, uterus, leeps, ( you get the idea) it is not anchored enough without a 
good dose of paraffin, causing more chatter when you section, and maybe 
chipping out more frequently, or even the whole bottom surface to lift off the 
cassette. I guess I have some pet peeves with this topic,  so thanks for 
letting me get that out!




Joelle Weaver MAOM, HTL (ASCP) QIHC
  From: valerie.han...@parrishmed.com
 To: billodonn...@catholichealth.net; histonet@lists.utsouthwestern.edu
 Date: Tue, 9 Oct 2012 10:51:01 -0400
 CC: 
 Subject: [Histonet] RE: Metal molds
 
 We clean our molds once a week. Soak them in Xylene to remove paraffin, soak 
 in 100% alcohol to remove xylene, rinse in running water, dry and spray with 
 mold release solution.
 
 Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief 
 Parrish Medical Center
 951 N. Washington Ave.
 Titusville, Florida 32976
 Phone:(321) 268-6333 ext. 7506
 Fax: (321) 268-6149
 valerie.han...@parrishmed.com
  
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
 O'Donnell, Bill
 Sent: Monday, October 08, 2012 4:32 PM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Metal molds
 
 

Re: [Histonet] RE: Metal molds

2012-10-10 Thread Jennifer Campbell
We clean ours in the VIP every Friday. We recycle our xylene substitute
from the VIP but you cannot recycle the cleaning alcohol.

They are all bright and shiny for Monday morning!

Jen Campbell

On Wed, Oct 10, 2012 at 9:46 AM, Bartlett, Jeanine (CDC/OID/NCEZID) 
j...@cdc.gov wrote:

 Does anyone that uses the VIP to clean their base molds also recycle the
 alcohols/xylene?  We were told that you should not clean the molds in the
 processor if you were recycling. Thanks!

 Jeanine H. Bartlett
 Centers for Disease Control and Prevention
 Infectious Diseases Pathology Branch
 404-639-3590
 jeanine.bartl...@cdc.hhs.gov

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harris, Diana
 Sent: Wednesday, October 10, 2012 9:42 AM
 To: 'susan.wal...@hcahealthcare.com'; joellewea...@hotmail.com;
 valerie.han...@parrishmed.com
 Cc: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] RE: Metal molds

 At our lab we clean the molds in the VIP cleaning cycle then dip in mold
 release also.  Works well.  The only precaution is to drain the molds well
 after dipping otherwise embedding can be effected.


 Diana Harris
 QC  Method Development Technologist
 Dept. Of Laboratory Medicine
 Anatomical Pathology
 Royal Jubilee Hospital
 Victoria, BC Canada



 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
 susan.wal...@hcahealthcare.com
 Sent: Wednesday, October 10, 2012 12:01 AM
 To: joellewea...@hotmail.com; valerie.han...@parrishmed.com
 Cc: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] RE: Metal molds

 We put our molds in the VIP before running the cleaning cycle daily. Then
 we dip them in alcohol containing mold release..air dry and store.

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu [mailto:
 histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
 Sent: Tuesday, October 09, 2012 3:27 PM
 To: valerie.han...@parrishmed.com
 Cc: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] RE: Metal molds


 I always cleaned them daily, either the very hot water, soapy water
 method, with water running over them in the sink with them on their sides
 so it passes over them, not upright so the water sits in them- then a rinse
 in alcohol and completely air dry. Or you can always do the clean cycle
 with the racks, running them through xylene, etc. They come out very clean
 this way- used an old processor that was a backup for this most of the
 time. But I always did them daily, but also wiped each one out with gauze
 if I used them twice in an embedding session ( for more than one specimen
 in that large batch). Also I like metal, I hate those plastic ones. If you
 keep the block face surface of the mold warm-hot,  and flatten before it
 turns completely white the specimen is at the surface and you are able to
 see the edges easily without a lot of facing. I think this saves time
 cutting through paraffin, and saves blades. Plus if the specimen is not
 flat enough, you see it right away and know if you must re-embed to get a
 complete, representative section, rather than after you have cut some
 superficial parts of some edges away and not others, only to have to
 re-embed anyhow. The other problems I see are when people are afraid of
 big molds- please if you are only taking one section, use one large enough
 to leave a perimeter. Don't try to squeeze it into a medium mold, you are
 unlikely to need multiple sections on one slide and it is much easier to
 get flat and get a good section.  Also please  put enough paraffin on top,
 so that when it is cool the layer over the grooves in the cassette is not
 so thin that you can clearly see the depressions. That little bit of
 paraffin is much cheaper than tech time in re-embedding and fussing with a
 block longer than you should.  Not so much a big issue for many specimens,
 but anything hard/ dense, such as bone, cervix, uterus, leeps, ( you get
 the idea) it is not anchored enough without a good dose of paraffin,
 causing more chatter when you section, and maybe chipping out more
 frequently, or even the whole bottom surface to lift off the cassette. I
 guess I have some pet peeves with this topic,  so thanks for letting me
 get that out!




 Joelle Weaver MAOM, HTL (ASCP) QIHC
   From: valerie.han...@parrishmed.com
  To: billodonn...@catholichealth.net; histonet@lists.utsouthwestern.edu
  Date: Tue, 9 Oct 2012 10:51:01 -0400
  CC:
  Subject: [Histonet] RE: Metal molds
 
  We clean our molds once a week. Soak them in Xylene to remove paraffin,
 soak in 100% alcohol to remove xylene, rinse in running water, dry and
 spray with mold release solution.
 
  Valerie A. Hannen, MLT(ASCP),HTL,SU(FL) Histology Section Chief
  Parrish Medical Center
  951 N. Washington Ave.
  Titusville, Florida 32976
  Phone:(321) 268-6333 ext. 7506
 

RE: [Histonet] RE: Metal molds

2012-10-10 Thread Brendal Finlay

We clean molds every day.  My preferred method is heated water to
melt the paraffin off, then allow to cool.  Peel the paraffin from
the surface of the water, remove the molds from the water, then dunk
them about 10 times in a mixture of alcohol and mold release.  Allow
to air dry or dry in a low temp oven.  


In my experience, molds that aren't cleaned on a regular basis make it
very difficult to remove the embedded cassettes even if very, very
cold.  It's easier for me if my workspace and tools are clean and
organized.


-Original message-
From: susan.wal...@hcahealthcare.com
Date: Wed, 10 Oct 2012 01:00:50 -0500
To: joellewea...@hotmail.com, valerie.han...@parrishmed.com
Subject: RE: [Histonet] RE: Metal molds

 We put our molds in the VIP before running the cleaning cycle daily.
Then we dip them in alcohol containing mold release..air dry and
store.
 
 -Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle
weaver
 Sent: Tuesday, October 09, 2012 3:27 PM
 To: valerie.han...@parrishmed.com
 Cc: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] RE: Metal molds
 
 
 I always cleaned them daily, either the very hot water, soapy water
method, with water running over them in the sink with them on their
sides so it passes over them, not upright so the water sits in them-
then a rinse in alcohol and completely air dry. Or you can always do
the clean cycle with the racks, running them through xylene, etc. They
come out very clean this way- used an old processor that was a backup
for this most of the time. But I always did them daily, but also wiped
each one out with gauze if I used them twice in an embedding session (
for more than one specimen in that large batch). Also I like metal, I
hate those plasticones. If you keep the block face surface of the mold
warm-hot, and flatten before it turns completely white the specimen is
at the surface and you are able to see the edges easily without a lot
of facing. I think this saves time cutting through paraffin, and
saves blades. Plus if the specimen is not flat enough, you see it
right away and know if you must re-embed to get a complete,
representative section, rather than after you have cut some
superficial parts of some edges away and not others, only to have to
re-embed anyhow. The other problems I see are when people are afraid
of big molds- please if you are only taking one section, use one large
enough to leave a perimeter. Don't try to squeeze it into a medium
mold, you are unlikely to need multiple sections on one slide and it
is much easier to get flat and get a good section. Also please put
enough paraffin on top, so that when it is cool the layer over the
grooves in the cassette is not so thin that youcan clearly see the
depressions. That little bit of paraffin is much cheaper than tech
time in re-embedding and fussing with a block longer than you should.
Not so much a big issue for many specimens, but anything hard/ dense,
such as bone, cervix, uterus, leeps, ( you get the idea) it is not
anchored enough without a good dose of paraffin, causing more chatter
when you section, and maybe chipping out more frequently, or even the
whole bottom surface to lift off the cassette. I guess I have some
pet peeves with this topic, so thanks for letting me get that out!
 
 
 
 
 Joelle Weaver MAOM, HTL (ASCP) QIHC
  From: valerie.han...@parrishmed.com
  To: billodonn...@catholichealth.net;
histonet@lists.utsouthwestern.edu
  Date: Tue, 9 Oct 2012 10:51:01 -0400
  CC: 
  Subject: [Histonet] RE: Metal molds
  
  We clean our molds once a week. Soakthem in Xylene to remove
paraffin, soak in 100% alcohol to remove xylene, rinse in running
water, dry and spray with mold release solution.
  
  Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
  Histology Section Chief
  Parrish Medical Center
  951 N. Washington Ave.
  Titusville, Florida 32976
  Phone:(321) 268-6333 ext. 7506
  Fax: (321) 268-6149
  valerie.han...@parrishmed.com
  
  
  -Original Message-
  From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
O'Donnell, Bill
  Sent: Monday, October 08, 2012 4:32 PM
  To: histonet@lists.utsouthwestern.edu
  Subject: [Histonet] Metal molds
  
  
  OK folks, I know I should be smarter than this and I haven't seen
discussion on itlately 
  
  Are people cleaning their metal embedding molds after evey
embedding session?
  
  If not, how often do you clean them? 
  
  Do you clean them at all?
  
  If you clean them, how do you do it? 
  
  Thanks
  
  Bill
  William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good
Samaritan Hospital 10 East 31st Street Kearney, NE 68847 
  
  SERENITY is not freedom from the storm, but peace amid the storm.
  
  Cultivate it in PRAYER!
  
  
  
  
  This electronic mail and any attached documents are intended
solely for the named addressee(s) and contain confidential

[Histonet] RE: Histonet Digest, Vol 107, Issue 14- CLEANING MOLDS

2012-10-10 Thread Karen Kay
Good Morning Bill,
We clean our molds once a week via the purge cycle in our tissue processor.  
They come out very clean and ready for a release spray after which they are 
stacked and placed back into the embedding unit. The molds are used for the 
week.  We alternate the cleaning cycles amongst the embedding centres so that 
all molds are not being cleaned at the same time. This system has worked well 
for us and has eliminated much of the manual aspect of performing this task.
It also greatly lessens the possibility of any small fragments of tissue that 
could be left behind if the tray is removed before the block has had long eough 
to totally cool.

Karen J Kay, MLT
Supervisor - Histopathology and Cytology Laboratory
Chinook Regional Hospital -South Zone West - Alberta Health Services
960 - 19 Street South
Lethbridge, Alberta - Canada
T1J 1W5
Phone: (403) 388-6061-  Fax: (403) 388-6067
karen@albertahealthservices.ca


Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Monday, October 08, 2012 2:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Metal molds


 OK folks, I know I should be smarter than this and I haven't seen discussion 
on it lately

Are people cleaning their metal embedding molds after evey embedding session?

If not, how often do you clean them?

Do you clean them at all?

If you clean them, how do you do it?

Thanks

Bill
William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan 
Hospital
10 East 31st Street
Kearney, NE 68847

SERENITY is not freedom from the storm, but peace amid the storm.

Cultivate it in PRAYER!

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Re: [Histonet] RE: Metal molds

2012-10-10 Thread Rene J Buesa
You can recycle the xylene  used in the cleaning cycle. I used to mix ALL the 
used xylene (from tissue processing + cleaning xylene) and recycle it without 
any problems. I did not recycle alcohol (not cost effective and time consuming).
René J.



From: Bartlett, Jeanine (CDC/OID/NCEZID) j...@cdc.gov
To: Harris, Diana diana.har...@viha.ca; 'susan.wal...@hcahealthcare.com' 
susan.wal...@hcahealthcare.com; joellewea...@hotmail.com 
joellewea...@hotmail.com; valerie.han...@parrishmed.com 
valerie.han...@parrishmed.com 
Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Wednesday, October 10, 2012 9:46 AM
Subject: RE: [Histonet] RE: Metal molds

Does anyone that uses the VIP to clean their base molds also recycle the 
alcohols/xylene?  We were told that you should not clean the molds in the 
processor if you were recycling. Thanks!

Jeanine H. Bartlett
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
404-639-3590
jeanine.bartl...@cdc.hhs.gov

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harris, Diana
Sent: Wednesday, October 10, 2012 9:42 AM
To: 'susan.wal...@hcahealthcare.com'; joellewea...@hotmail.com; 
valerie.han...@parrishmed.com
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds

At our lab we clean the molds in the VIP cleaning cycle then dip in mold 
release also.  Works well.  The only precaution is to drain the molds well 
after dipping otherwise embedding can be effected.


Diana Harris
QC  Method Development Technologist
Dept. Of Laboratory Medicine
Anatomical Pathology
Royal Jubilee Hospital
Victoria, BC Canada



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
susan.wal...@hcahealthcare.com
Sent: Wednesday, October 10, 2012 12:01 AM
To: joellewea...@hotmail.com; valerie.han...@parrishmed.com
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds

We put our molds in the VIP before running the cleaning cycle daily. Then we 
dip them in alcohol containing mold release..air dry and store.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Tuesday, October 09, 2012 3:27 PM
To: valerie.han...@parrishmed.com
Cc: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds


I always cleaned them daily, either the very hot water, soapy water method, 
with water running over them in the sink with them on their sides so it passes 
over them, not upright so the water sits in them- then a rinse in alcohol and 
completely air dry. Or you can always do the clean cycle with the racks, 
running them through xylene, etc. They come out very clean this way- used an 
old processor that was a backup for this most of the time. But I always did 
them daily, but also wiped each one out with gauze if I used them twice in an 
embedding session ( for more than one specimen in that large batch). Also I 
like metal, I hate those plastic ones. If you keep the block face surface of 
the mold warm-hot,  and flatten before it turns completely white the specimen 
is at the surface and you are able to see the edges easily without a lot of 
facing. I think this saves time cutting through paraffin, and saves blades. 
Plus if the specimen is not flat enough, you
 see it right away and know if you must re-embed to get a complete, 
representative section, rather than after you have cut some superficial parts 
of some edges away and not others, only to have to re-embed anyhow. The other 
problems I see are when people are afraid of big molds- please if you are 
only taking one section, use one large enough to leave a perimeter. Don't try 
to squeeze it into a medium mold, you are unlikely to need multiple sections on 
one slide and it is much easier to get flat and get a good section.  Also 
please  put enough paraffin on top, so that when it is cool the layer over the 
grooves in the cassette is not so thin that you can clearly see the 
depressions. That little bit of paraffin is much cheaper than tech time in 
re-embedding and fussing with a block longer than you should.  Not so much a 
big issue for many specimens, but anything hard/ dense, such as bone, cervix, 
uterus, leeps, ( you get the idea) it is not anchored
 enough without a good dose of paraffin, causing more chatter when you section, 
and maybe chipping out more frequently, or even the whole bottom surface to 
lift off the cassette. I guess I have some pet peeves with this topic,  so 
thanks for letting me get that out!




Joelle Weaver MAOM, HTL (ASCP) QIHC
 From: valerie.han...@parrishmed.com
 To: billodonn...@catholichealth.net; histonet@lists.utsouthwestern.edu
 Date: Tue, 9 Oct 2012 10:51:01 -0400
 CC: 
 Subject: 

RE: [Histonet] Facility Name on Slides

2012-10-10 Thread histotech
Jesus,

Thanks for the reply.  :)

I still have someone insisting that we *must* have the facility name on the
slide labels.  So, I am hoping I can get some others to chime in here, so I
can show a general consensus on this issue.  Either that or have links to
requirement lists for this item.

Thanks,
Michelle

-Original Message-
From: Jesus Ellin [mailto:jel...@yumaregional.org] 
Sent: Monday, October 08, 2012 7:41 PM
To: histot...@imagesbyhopper.com
Cc: Histonet@Lists. Utsouthwestern. Edu
Subject: Re: [Histonet] Facility Name on Slides

To my knowledge there is no regulation rather than having 2 identifiers,, 

Sent from my iPad

On Oct 8, 2012, at 1:46 PM, histot...@imagesbyhopper.com
histot...@imagesbyhopper.com wrote:

 Does anyone have any regs that you can point me to for both Joint
Commission and CAP which details definitively whether or not a facility name
is *required* to be included on slides? 
 
 I know it's a good idea, the larger question here is whether or not there
is a specific regulation for it?
 
 Thanks for all your help!
 Michelle
 
 
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are not the named recipient(s), please notify the sender at either the
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RE: [Histonet] RE: Metal molds

2012-10-10 Thread Goins, Tresa
I knew I might catch some grief for not cleaning our molds for five years, but 
my workplace is clean and organized.

Some things just work better without being cleaned with soap and water - I have 
a fifteen year old cast iron skillet that is as non-stick as Teflon due to a 
natural patina - maybe the paraffin blocks easy release from my un-clean 
molds” works for the same reason.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brendal Finlay
Sent: Wednesday, October 10, 2012 8:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds


We clean molds every day.  My preferred method is heated water to melt the 
paraffin off, then allow to cool.  Peel the paraffin from the surface of the 
water, remove the molds from the water, then dunk them about 10 times in a 
mixture of alcohol and mold release.  Allow to air dry or dry in a low temp 
oven.


In my experience, molds that aren't cleaned on a regular basis make it very 
difficult to remove the embedded cassettes even if very, very cold.  It's 
easier for me if my workspace and tools are clean and organized.

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RE: [Histonet] Facility Name on Slides

2012-10-10 Thread Blazek, Linda
I think what the CLIA regulations says it there must be two identifiers.  
Though there is a regulation that the report has the facility name and address 
on it. 
Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histot...@imagesbyhopper.com
Sent: Wednesday, October 10, 2012 12:47 PM
To: 'Jesus Ellin'
Cc: 'Histonet@Lists. Utsouthwestern. Edu'
Subject: RE: [Histonet] Facility Name on Slides

Jesus,

Thanks for the reply.  :)

I still have someone insisting that we *must* have the facility name on the 
slide labels.  So, I am hoping I can get some others to chime in here, so I can 
show a general consensus on this issue.  Either that or have links to 
requirement lists for this item.

Thanks,
Michelle

-Original Message-
From: Jesus Ellin [mailto:jel...@yumaregional.org]
Sent: Monday, October 08, 2012 7:41 PM
To: histot...@imagesbyhopper.com
Cc: Histonet@Lists. Utsouthwestern. Edu
Subject: Re: [Histonet] Facility Name on Slides

To my knowledge there is no regulation rather than having 2 identifiers,, 

Sent from my iPad

On Oct 8, 2012, at 1:46 PM, histot...@imagesbyhopper.com
histot...@imagesbyhopper.com wrote:

 Does anyone have any regs that you can point me to for both Joint
Commission and CAP which details definitively whether or not a facility name is 
*required* to be included on slides? 
 
 I know it's a good idea, the larger question here is whether or not 
 there
is a specific regulation for it?
 
 Thanks for all your help!
 Michelle
 
 
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

__
This message is confidential, intended only for the named
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disclosure under applicable law.  If you are not the intended recipient(s), you 
are notified that the dissemination, distribution, or copying of this message 
is strictly prohibited.  If you receive this message in error, or are not the 
named recipient(s), please notify the sender at either the e-mail, fax, 
address, or telephone number listed above and delete this e-mail from your 
computer. 
Thank You.
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RE: [Histonet] Facility Name on Slides

2012-10-10 Thread Morken, Timothy
I don't think there is a requirement, ie regulation. However, unless you can 
positively ID a slide as yours, or as your patient, without the facility name, 
slides could be confused with other institutions. So it is a good practice in 
any case. 

This is something you can make as internal policy, one way or the other, with 
the consideration of how you identify your institutions slides if there is no 
institution name on the slide or slide label.

Tim Morken

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histot...@imagesbyhopper.com
Sent: Wednesday, October 10, 2012 9:47 AM
To: 'Jesus Ellin'
Cc: 'Histonet@Lists. Utsouthwestern. Edu'
Subject: RE: [Histonet] Facility Name on Slides

Jesus,

Thanks for the reply.  :)

I still have someone insisting that we *must* have the facility name on the 
slide labels.  So, I am hoping I can get some others to chime in here, so I can 
show a general consensus on this issue.  Either that or have links to 
requirement lists for this item.

Thanks,
Michelle

-Original Message-
From: Jesus Ellin [mailto:jel...@yumaregional.org]
Sent: Monday, October 08, 2012 7:41 PM
To: histot...@imagesbyhopper.com
Cc: Histonet@Lists. Utsouthwestern. Edu
Subject: Re: [Histonet] Facility Name on Slides

To my knowledge there is no regulation rather than having 2 identifiers,, 

Sent from my iPad

On Oct 8, 2012, at 1:46 PM, histot...@imagesbyhopper.com
histot...@imagesbyhopper.com wrote:

 Does anyone have any regs that you can point me to for both Joint
Commission and CAP which details definitively whether or not a facility name is 
*required* to be included on slides? 
 
 I know it's a good idea, the larger question here is whether or not 
 there
is a specific regulation for it?
 
 Thanks for all your help!
 Michelle
 
 
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet

__
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disclosure under applicable law.  If you are not the intended recipient(s), you 
are notified that the dissemination, distribution, or copying of this message 
is strictly prohibited.  If you receive this message in error, or are not the 
named recipient(s), please notify the sender at either the e-mail, fax, 
address, or telephone number listed above and delete this e-mail from your 
computer. 
Thank You.
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RE: [Histonet] RE: Metal molds

2012-10-10 Thread Fred Underwood
You're making me hungry Tresa.  I bet that skillet makes a killer batch
of hash browns.

 Goins, Tresa tgo...@mt.gov 10/10/2012 12:53 PM 
I knew I might catch some grief for not cleaning our molds for five
years, but my workplace is clean and organized.

Some things just work better without being cleaned with soap and water
- I have a fifteen year old cast iron skillet that is as non-stick as
Teflon due to a natural patina - maybe the paraffin blocks easy release
from my un-clean molds* works for the same reason.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brendal
Finlay
Sent: Wednesday, October 10, 2012 8:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds


We clean molds every day.  My preferred method is heated water to melt
the paraffin off, then allow to cool.  Peel the paraffin from the
surface of the water, remove the molds from the water, then dunk them
about 10 times in a mixture of alcohol and mold release.  Allow to air
dry or dry in a low temp oven.


In my experience, molds that aren't cleaned on a regular basis make it
very difficult to remove the embedded cassettes even if very, very cold.
 It's easier for me if my workspace and tools are clean and organized.

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RE: [Histonet] RE: Metal molds

2012-10-10 Thread Bartlett, Jeanine (CDC/OID/NCEZID)
I'm thinking some cracklin cornbread!

Jeanine H. Bartlett
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
404-639-3590
jeanine.bartl...@cdc.hhs.gov

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fred Underwood
Sent: Wednesday, October 10, 2012 1:29 PM
To: histonet@lists.utsouthwestern.edu; Brendal Finlay; Tresa Goins
Subject: RE: [Histonet] RE: Metal molds

You're making me hungry Tresa.  I bet that skillet makes a killer batch of hash 
browns.

 Goins, Tresa tgo...@mt.gov 10/10/2012 12:53 PM 
I knew I might catch some grief for not cleaning our molds for five years, but 
my workplace is clean and organized.

Some things just work better without being cleaned with soap and water
- I have a fifteen year old cast iron skillet that is as non-stick as Teflon 
due to a natural patina - maybe the paraffin blocks easy release from my 
un-clean molds* works for the same reason.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brendal Finlay
Sent: Wednesday, October 10, 2012 8:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds


We clean molds every day.  My preferred method is heated water to melt the 
paraffin off, then allow to cool.  Peel the paraffin from the surface of the 
water, remove the molds from the water, then dunk them about 10 times in a 
mixture of alcohol and mold release.  Allow to air dry or dry in a low temp 
oven.


In my experience, molds that aren't cleaned on a regular basis make it very 
difficult to remove the embedded cassettes even if very, very cold.
 It's easier for me if my workspace and tools are clean and organized.


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RE: [Histonet] RE: Metal molds

2012-10-10 Thread Blazek, Linda
Ok  that did it...  I'm going home and making cornbread, hash browns with 
onions, sausage and green pepper.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, 
Jeanine (CDC/OID/NCEZID)
Sent: Wednesday, October 10, 2012 1:30 PM
To: Fred Underwood; histonet@lists.utsouthwestern.edu; Brendal Finlay; Tresa 
Goins
Subject: RE: [Histonet] RE: Metal molds

I'm thinking some cracklin cornbread!

Jeanine H. Bartlett
Centers for Disease Control and Prevention Infectious Diseases Pathology Branch
404-639-3590
jeanine.bartl...@cdc.hhs.gov

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fred Underwood
Sent: Wednesday, October 10, 2012 1:29 PM
To: histonet@lists.utsouthwestern.edu; Brendal Finlay; Tresa Goins
Subject: RE: [Histonet] RE: Metal molds

You're making me hungry Tresa.  I bet that skillet makes a killer batch of hash 
browns.

 Goins, Tresa tgo...@mt.gov 10/10/2012 12:53 PM 
I knew I might catch some grief for not cleaning our molds for five years, but 
my workplace is clean and organized.

Some things just work better without being cleaned with soap and water
- I have a fifteen year old cast iron skillet that is as non-stick as Teflon 
due to a natural patina - maybe the paraffin blocks easy release from my 
un-clean molds* works for the same reason.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brendal Finlay
Sent: Wednesday, October 10, 2012 8:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds


We clean molds every day.  My preferred method is heated water to melt the 
paraffin off, then allow to cool.  Peel the paraffin from the surface of the 
water, remove the molds from the water, then dunk them about 10 times in a 
mixture of alcohol and mold release.  Allow to air dry or dry in a low temp 
oven.


In my experience, molds that aren't cleaned on a regular basis make it very 
difficult to remove the embedded cassettes even if very, very cold.
 It's easier for me if my workspace and tools are clean and organized.


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RE: [Histonet] RE: Metal molds

2012-10-10 Thread Goins, Tresa
Dag' nabbit'  Now I'm hungry . . . 

Tresa

-Original Message-
From: Blazek, Linda [mailto:lbla...@digestivespecialists.com] 
Sent: Wednesday, October 10, 2012 11:38 AM
To: 'Bartlett, Jeanine (CDC/OID/NCEZID)'; Fred Underwood; 
histonet@lists.utsouthwestern.edu; Brendal Finlay; Goins, Tresa
Subject: RE: [Histonet] RE: Metal molds

Ok  that did it...  I'm going home and making cornbread, hash browns with 
onions, sausage and green pepper.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bartlett, 
Jeanine (CDC/OID/NCEZID)
Sent: Wednesday, October 10, 2012 1:30 PM
To: Fred Underwood; histonet@lists.utsouthwestern.edu; Brendal Finlay; Tresa 
Goins
Subject: RE: [Histonet] RE: Metal molds

I'm thinking some cracklin cornbread!

Jeanine H. Bartlett
Centers for Disease Control and Prevention Infectious Diseases Pathology Branch
404-639-3590
jeanine.bartl...@cdc.hhs.gov

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fred Underwood
Sent: Wednesday, October 10, 2012 1:29 PM
To: histonet@lists.utsouthwestern.edu; Brendal Finlay; Tresa Goins
Subject: RE: [Histonet] RE: Metal molds

You're making me hungry Tresa.  I bet that skillet makes a killer batch of hash 
browns.

 Goins, Tresa tgo...@mt.gov 10/10/2012 12:53 PM 
I knew I might catch some grief for not cleaning our molds for five years, but 
my workplace is clean and organized.

Some things just work better without being cleaned with soap and water
- I have a fifteen year old cast iron skillet that is as non-stick as Teflon 
due to a natural patina - maybe the paraffin blocks easy release from my 
un-clean molds* works for the same reason.

Tresa

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brendal Finlay
Sent: Wednesday, October 10, 2012 8:27 AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds


We clean molds every day.  My preferred method is heated water to melt the 
paraffin off, then allow to cool.  Peel the paraffin from the surface of the 
water, remove the molds from the water, then dunk them about 10 times in a 
mixture of alcohol and mold release.  Allow to air dry or dry in a low temp 
oven.


In my experience, molds that aren't cleaned on a regular basis make it very 
difficult to remove the embedded cassettes even if very, very cold.
 It's easier for me if my workspace and tools are clean and organized.


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RE: [Histonet] RE: Metal molds

2012-10-10 Thread joelle weaver

My impression of the intial thread was the general cleanliness and cross 
contamination potential, not the fact that the blocks aren't easily released. I 
have never had that issue. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
  Date: Wed, 10 Oct 2012 09:27:09 -0500
 From: brendal.fin...@medicalcenterclinic.com
 To: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] RE: Metal molds
 
 
 We clean molds every day.  My preferred method is heated water to
 melt the paraffin off, then allow to cool.  Peel the paraffin from
 the surface of the water, remove the molds from the water, then dunk
 them about 10 times in a mixture of alcohol and mold release.  Allow
 to air dry or dry in a low temp oven.  
 
 
 In my experience, molds that aren't cleaned on a regular basis make it
 very difficult to remove the embedded cassettes even if very, very
 cold.  It's easier for me if my workspace and tools are clean and
 organized.
 
 
 -Original message-
 From: susan.wal...@hcahealthcare.com
 Date: Wed, 10 Oct 2012 01:00:50 -0500
 To: joellewea...@hotmail.com, valerie.han...@parrishmed.com
 Subject: RE: [Histonet] RE: Metal molds
 
  We put our molds in the VIP before running the cleaning cycle daily.
 Then we dip them in alcohol containing mold release..air dry and
 store.
  
  -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle
 weaver
  Sent: Tuesday, October 09, 2012 3:27 PM
  To: valerie.han...@parrishmed.com
  Cc: histonet@lists.utsouthwestern.edu
  Subject: RE: [Histonet] RE: Metal molds
  
  
  I always cleaned them daily, either the very hot water, soapy water
 method, with water running over them in the sink with them on their
 sides so it passes over them, not upright so the water sits in them-
 then a rinse in alcohol and completely air dry. Or you can always do
 the clean cycle with the racks, running them through xylene, etc. They
 come out very clean this way- used an old processor that was a backup
 for this most of the time. But I always did them daily, but also wiped
 each one out with gauze if I used them twice in an embedding session (
 for more than one specimen in that large batch). Also I like metal, I
 hate those plasticones. If you keep the block face surface of the mold
 warm-hot, and flatten before it turns completely white the specimen is
 at the surface and you are able to see the edges easily without a lot
 of facing. I think this saves time cutting through paraffin, and
 saves blades. Plus if the specimen is not flat enough, you see it
 right away and know if you must re-embed to get a complete,
 representative section, rather than after you have cut some
 superficial parts of some edges away and not others, only to have to
 re-embed anyhow. The other problems I see are when people are afraid
 of big molds- please if you are only taking one section, use one large
 enough to leave a perimeter. Don't try to squeeze it into a medium
 mold, you are unlikely to need multiple sections on one slide and it
 is much easier to get flat and get a good section. Also please put
 enough paraffin on top, so that when it is cool the layer over the
 grooves in the cassette is not so thin that youcan clearly see the
 depressions. That little bit of paraffin is much cheaper than tech
 time in re-embedding and fussing with a block longer than you should.
 Not so much a big issue for many specimens, but anything hard/ dense,
 such as bone, cervix, uterus, leeps, ( you get the idea) it is not
 anchored enough without a good dose of paraffin, causing more chatter
 when you section, and maybe chipping out more frequently, or even the
 whole bottom surface to lift off the cassette. I guess I have some
 pet peeves with this topic, so thanks for letting me get that out!
  
  
  
  
  Joelle Weaver MAOM, HTL (ASCP) QIHC
   From: valerie.han...@parrishmed.com
   To: billodonn...@catholichealth.net;
 histonet@lists.utsouthwestern.edu
   Date: Tue, 9 Oct 2012 10:51:01 -0400
   CC: 
   Subject: [Histonet] RE: Metal molds
   
   We clean our molds once a week. Soakthem in Xylene to remove
 paraffin, soak in 100% alcohol to remove xylene, rinse in running
 water, dry and spray with mold release solution.
   
   Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
   Histology Section Chief
   Parrish Medical Center
   951 N. Washington Ave.
   Titusville, Florida 32976
   Phone:(321) 268-6333 ext. 7506
   Fax: (321) 268-6149
   valerie.han...@parrishmed.com
   
   
   -Original Message-
   From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
 O'Donnell, Bill
   Sent: Monday, October 08, 2012 4:32 PM
   To: histonet@lists.utsouthwestern.edu
   Subject: [Histonet] Metal molds
   
   
   OK folks, I know I should be smarter than this and I haven't seen
 discussion on itlately 
   
   Are people cleaning their metal embedding molds after evey
 embedding session?
  

RE: [Histonet] RE: Metal molds

2012-10-10 Thread O'Donnell, Bill
Actually, the thread began because our VIP rotary valve got filled with
wax and required service. We clean daily in VIP, but wanted to know what
others were doing. Mostly, people are using the VIP, so we may need to
modify our regularity or change out cleaning solution more frequently.

I do like where the thread has gone. I got my (chicken fryer) cast iron
skillet from my mother who got it from her mother. They were migrant
workers during the dust bowl,  picking cotton in Texas and oranges in
California before settling in Arizona. I remember Mom frying chicken (or
the occasional rabbit) in it on Sunday mornings. Yes, I still use it,
but I have added several other cast iron pieces to the arsenal including
a bread pan! There will be pound cake in the oven tonight!

William (Bill) O'Donnell, HT (ASCP) QIHC 
Senior Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.

Cultivate it in PRAYER!

 




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle
weaver
Sent: Wednesday, October 10, 2012 12:42 PM
To: brendal.fin...@medicalcenterclinic.com;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds


My impression of the intial thread was the general cleanliness and cross
contamination potential, not the fact that the blocks aren't easily
released. I have never had that issue. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
  Date: Wed, 10 Oct 2012 09:27:09 -0500
 From: brendal.fin...@medicalcenterclinic.com
 To: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] RE: Metal molds
 
 
 We clean molds every day.  My preferred method is heated water to melt

 the paraffin off, then allow to cool.  Peel the paraffin from the 
 surface of the water, remove the molds from the water, then dunk them 
 about 10 times in a mixture of alcohol and mold release.  Allow to air

 dry or dry in a low temp oven.
 
 
 In my experience, molds that aren't cleaned on a regular basis make it

 very difficult to remove the embedded cassettes even if very, very 
 cold.  It's easier for me if my workspace and tools are clean and 
 organized.
 
 
 -Original message-
 From: susan.wal...@hcahealthcare.com
 Date: Wed, 10 Oct 2012 01:00:50 -0500
 To: joellewea...@hotmail.com, valerie.han...@parrishmed.com
 Subject: RE: [Histonet] RE: Metal molds
 
  We put our molds in the VIP before running the cleaning cycle daily.
 Then we dip them in alcohol containing mold release..air dry and 
 store.
  
  -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle

 weaver
  Sent: Tuesday, October 09, 2012 3:27 PM
  To: valerie.han...@parrishmed.com
  Cc: histonet@lists.utsouthwestern.edu
  Subject: RE: [Histonet] RE: Metal molds
  
  
  I always cleaned them daily, either the very hot water, soapy water
 method, with water running over them in the sink with them on their 
 sides so it passes over them, not upright so the water sits in them- 
 then a rinse in alcohol and completely air dry. Or you can always do 
 the clean cycle with the racks, running them through xylene, etc. They

 come out very clean this way- used an old processor that was a backup 
 for this most of the time. But I always did them daily, but also wiped

 each one out with gauze if I used them twice in an embedding session (

 for more than one specimen in that large batch). Also I like metal, I 
 hate those plasticones. If you keep the block face surface of the mold

 warm-hot, and flatten before it turns completely white the specimen is

 at the surface and you are able to see the edges easily without a lot 
 of facing. I think this saves time cutting through paraffin, and 
 saves blades. Plus if the specimen is not flat enough, you see it 
 right away and know if you must re-embed to get a complete, 
 representative section, rather than after you have cut some 
 superficial parts of some edges away and not others, only to have to 
 re-embed anyhow. The other problems I see are when people are afraid
 of big molds- please if you are only taking one section, use one large

 enough to leave a perimeter. Don't try to squeeze it into a medium 
 mold, you are unlikely to need multiple sections on one slide and it 
 is much easier to get flat and get a good section. Also please put 
 enough paraffin on top, so that when it is cool the layer over the 
 grooves in the cassette is not so thin that youcan clearly see the 
 depressions. That little bit of paraffin is much cheaper than tech 
 time in re-embedding and fussing with a block longer than you should.
 Not so much a big issue for many specimens, but anything hard/ dense, 
 such as bone, cervix, uterus, leeps, ( you get the idea) it is not 
 anchored enough without a good dose of paraffin, causing more chatter 
 when you section, and 

RE: [Histonet] RE: Metal molds

2012-10-10 Thread Weems, Joyce K.
You all have me starving..

But I believe the manufacture recommends that cassettes not be cleaned in the 
processor - sticky valves being the reason.

We haven't cleaned cassettes in years or used mold release. They go back in the 
embedding center hot compartment - standing on edge. I ask frequently, but we 
have no issues with floaters.

But I USED to clean - thought it had to be done - boiled in soap let cool, 
rinsed, dried, choked to death on mold release spray.. all the good stuff. Just 
had to listen to coworkers that had a different experience!!

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Wednesday, October 10, 2012 2:19 PM
To: joelle weaver; brendal.fin...@medicalcenterclinic.com; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds

Actually, the thread began because our VIP rotary valve got filled with wax and 
required service. We clean daily in VIP, but wanted to know what others were 
doing. Mostly, people are using the VIP, so we may need to modify our 
regularity or change out cleaning solution more frequently.

I do like where the thread has gone. I got my (chicken fryer) cast iron skillet 
from my mother who got it from her mother. They were migrant workers during the 
dust bowl,  picking cotton in Texas and oranges in California before settling 
in Arizona. I remember Mom frying chicken (or the occasional rabbit) in it on 
Sunday mornings. Yes, I still use it, but I have added several other cast iron 
pieces to the arsenal including a bread pan! There will be pound cake in the 
oven tonight!

William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan 
Hospital
10 East 31st Street
Kearney, NE 68847

SERENITY is not freedom from the storm, but peace amid the storm.

Cultivate it in PRAYER!






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Wednesday, October 10, 2012 12:42 PM
To: brendal.fin...@medicalcenterclinic.com;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds


My impression of the intial thread was the general cleanliness and cross 
contamination potential, not the fact that the blocks aren't easily released. I 
have never had that issue.




Joelle Weaver MAOM, HTL (ASCP) QIHC
  Date: Wed, 10 Oct 2012 09:27:09 -0500
 From: brendal.fin...@medicalcenterclinic.com
 To: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] RE: Metal molds


 We clean molds every day.  My preferred method is heated water to melt

 the paraffin off, then allow to cool.  Peel the paraffin from the
 surface of the water, remove the molds from the water, then dunk them
 about 10 times in a mixture of alcohol and mold release.  Allow to air

 dry or dry in a low temp oven.


 In my experience, molds that aren't cleaned on a regular basis make it

 very difficult to remove the embedded cassettes even if very, very
 cold.  It's easier for me if my workspace and tools are clean and
 organized.


 -Original message-
 From: susan.wal...@hcahealthcare.com
 Date: Wed, 10 Oct 2012 01:00:50 -0500
 To: joellewea...@hotmail.com, valerie.han...@parrishmed.com
 Subject: RE: [Histonet] RE: Metal molds

  We put our molds in the VIP before running the cleaning cycle daily.
 Then we dip them in alcohol containing mold release..air dry and
 store.
 
  -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle

 weaver
  Sent: Tuesday, October 09, 2012 3:27 PM
  To: valerie.han...@parrishmed.com
  Cc: histonet@lists.utsouthwestern.edu
  Subject: RE: [Histonet] RE: Metal molds
 
 
  I always cleaned them daily, either the very hot water, soapy water
 method, with water running over them in the sink with them on their
 sides so it passes over them, not upright so the water sits in them-
 then a rinse in alcohol and completely air dry. Or you can always do
 the clean cycle with the racks, running them through xylene, etc. They

 come out very clean this way- used an old processor that was a backup
 for this most of the time. But I always did them daily, but also wiped

 each one out with gauze if I used them twice in an embedding session (

 for 

RE: [Histonet] RE: Metal molds

2012-10-10 Thread Blazek, Linda
I too haven't cleaned molds in years and have not had a problem.  Standing them 
on edge in the embedding center seems to work just fine.  I'm not sure how 
cleaning the molds in the processor could create sticky valves though as long 
as you are changing your cleaning xylene on a regular basis.  

I'm REALLY hungry now..  I have a new addition to my cast iron pans!  It's a 
Dutch oven. Apple cake in it is great! It's wonderful addition but it sure 
weighs a lot!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Wednesday, October 10, 2012 2:34 PM
To: 'O'Donnell, Bill'; joelle weaver; brendal.fin...@medicalcenterclinic.com; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds

You all have me starving..

But I believe the manufacture recommends that cassettes not be cleaned in the 
processor - sticky valves being the reason.

We haven't cleaned cassettes in years or used mold release. They go back in the 
embedding center hot compartment - standing on edge. I ask frequently, but we 
have no issues with floaters.

But I USED to clean - thought it had to be done - boiled in soap let cool, 
rinsed, dried, choked to death on mold release spray.. all the good stuff. Just 
had to listen to coworkers that had a different experience!!

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph's 
Hospital and is intended for the sole use of the intended recipient(s).  It may 
contain information that is privileged and confidential.  Any unauthorized 
review, use, disclosure, or distribution is prohibited. If you are not the 
intended recipient, please delete this message, and reply to the sender 
regarding the error in a separate email.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Wednesday, October 10, 2012 2:19 PM
To: joelle weaver; brendal.fin...@medicalcenterclinic.com; 
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds

Actually, the thread began because our VIP rotary valve got filled with wax and 
required service. We clean daily in VIP, but wanted to know what others were 
doing. Mostly, people are using the VIP, so we may need to modify our 
regularity or change out cleaning solution more frequently.

I do like where the thread has gone. I got my (chicken fryer) cast iron skillet 
from my mother who got it from her mother. They were migrant workers during the 
dust bowl,  picking cotton in Texas and oranges in California before settling 
in Arizona. I remember Mom frying chicken (or the occasional rabbit) in it on 
Sunday mornings. Yes, I still use it, but I have added several other cast iron 
pieces to the arsenal including a bread pan! There will be pound cake in the 
oven tonight!

William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan 
Hospital
10 East 31st Street
Kearney, NE 68847

SERENITY is not freedom from the storm, but peace amid the storm.

Cultivate it in PRAYER!






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Wednesday, October 10, 2012 12:42 PM
To: brendal.fin...@medicalcenterclinic.com;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds


My impression of the intial thread was the general cleanliness and cross 
contamination potential, not the fact that the blocks aren't easily released. I 
have never had that issue.




Joelle Weaver MAOM, HTL (ASCP) QIHC
  Date: Wed, 10 Oct 2012 09:27:09 -0500
 From: brendal.fin...@medicalcenterclinic.com
 To: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] RE: Metal molds


 We clean molds every day.  My preferred method is heated water to melt

 the paraffin off, then allow to cool.  Peel the paraffin from the 
 surface of the water, remove the molds from the water, then dunk them 
 about 10 times in a mixture of alcohol and mold release.  Allow to air

 dry or dry in a low temp oven.


 In my experience, molds that aren't cleaned on a regular basis make it

 very difficult to remove the embedded cassettes even if very, very 
 cold.  It's easier for me if my workspace and tools are clean and 
 organized.


 -Original message-
 From: susan.wal...@hcahealthcare.com
 Date: Wed, 10 Oct 2012 01:00:50 -0500
 To: joellewea...@hotmail.com, valerie.han...@parrishmed.com
 Subject: RE: [Histonet] RE: Metal molds

  We put our molds in the VIP before running the cleaning cycle daily.
 Then we dip them in alcohol containing mold release..air dry and 
 store.
 
  -Original Message-
 From: 

Re: [Histonet] RE: Metal molds

2012-10-10 Thread Rene J Buesa
You now nailed on the head: what you need to do is change more frequently the 
cleaning xylene.
I used to rotate the xylenes (before I changed to mineral oil) every time our 
VIP processed the amount of blocks for its capacity (300 for a VIP 300) and the 
rotation included also the cleaning xylene that would receive the first xylene.
The rotary valve cannot get clogged if you follow that protocol.
René J.



From: O'Donnell, Bill billodonn...@catholichealth.net
To: joelle weaver joellewea...@hotmail.com; 
brendal.fin...@medicalcenterclinic.com; histonet@lists.utsouthwestern.edu 
Sent: Wednesday, October 10, 2012 2:18 PM
Subject: RE: [Histonet] RE: Metal molds

Actually, the thread began because our VIP rotary valve got filled with
wax and required service. We clean daily in VIP, but wanted to know what
others were doing. Mostly, people are using the VIP, so we may need to
modify our regularity or change out cleaning solution more frequently.

I do like where the thread has gone. I got my (chicken fryer) cast iron
skillet from my mother who got it from her mother. They were migrant
workers during the dust bowl,  picking cotton in Texas and oranges in
California before settling in Arizona. I remember Mom frying chicken (or
the occasional rabbit) in it on Sunday mornings. Yes, I still use it,
but I have added several other cast iron pieces to the arsenal including
a bread pan! There will be pound cake in the oven tonight!

William (Bill) O'Donnell, HT (ASCP) QIHC 
Senior Histologist
Good Samaritan Hospital
10 East 31st Street
Kearney, NE 68847 

SERENITY is not freedom from the storm, but peace amid the storm.

Cultivate it in PRAYER!






-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle
weaver
Sent: Wednesday, October 10, 2012 12:42 PM
To: brendal.fin...@medicalcenterclinic.com;
histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Metal molds


My impression of the intial thread was the general cleanliness and cross
contamination potential, not the fact that the blocks aren't easily
released. I have never had that issue. 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 Date: Wed, 10 Oct 2012 09:27:09 -0500
 From: brendal.fin...@medicalcenterclinic.com
 To: histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] RE: Metal molds
 
 
 We clean molds every day.  My preferred method is heated water to melt

 the paraffin off, then allow to cool.  Peel the paraffin from the 
 surface of the water, remove the molds from the water, then dunk them 
 about 10 times in a mixture of alcohol and mold release.  Allow to air

 dry or dry in a low temp oven.
 
 
 In my experience, molds that aren't cleaned on a regular basis make it

 very difficult to remove the embedded cassettes even if very, very 
 cold.  It's easier for me if my workspace and tools are clean and 
 organized.
 
 
 -Original message-
 From: susan.wal...@hcahealthcare.com
 Date: Wed, 10 Oct 2012 01:00:50 -0500
 To: joellewea...@hotmail.com, valerie.han...@parrishmed.com
 Subject: RE: [Histonet] RE: Metal molds
 
  We put our molds in the VIP before running the cleaning cycle daily.
 Then we dip them in alcohol containing mold release..air dry and 
 store.
  
  -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle

 weaver
  Sent: Tuesday, October 09, 2012 3:27 PM
  To: valerie.han...@parrishmed.com
  Cc: histonet@lists.utsouthwestern.edu
  Subject: RE: [Histonet] RE: Metal molds
  
  
  I always cleaned them daily, either the very hot water, soapy water
 method, with water running over them in the sink with them on their 
 sides so it passes over them, not upright so the water sits in them- 
 then a rinse in alcohol and completely air dry. Or you can always do 
 the clean cycle with the racks, running them through xylene, etc. They

 come out very clean this way- used an old processor that was a backup 
 for this most of the time. But I always did them daily, but also wiped

 each one out with gauze if I used them twice in an embedding session (

 for more than one specimen in that large batch). Also I like metal, I 
 hate those plasticones. If you keep the block face surface of the mold

 warm-hot, and flatten before it turns completely white the specimen is

 at the surface and you are able to see the edges easily without a lot 
 of facing. I think this saves time cutting through paraffin, and 
 saves blades. Plus if the specimen is not flat enough, you see it 
 right away and know if you must re-embed to get a complete, 
 representative section, rather than after you have cut some 
 superficial parts of some edges away and not others, only to have to 
 re-embed anyhow. The other problems I see are when people are afraid
 of big molds- please if you are only taking one section, use one large

 enough to leave a 

[Histonet] Are those sites using the Thermo Fisher Slide Mate slide printer also using Thermo slides?

2012-10-10 Thread Harrison, Sandra C.
I just purchased 6 Slide Mates.  We just began using them yesterday. 

 

Are any of you using slides that are NOT Fisher slides on your
SlideMates?  

 

When you respond, could you answer the following questions?

a)  Is the print job consistently satisfactory?

b)  How long have had your SlideMate(s)?

c)   What difficulties did you have, getting it to consistently
print well?

d)  Approximately how many slides per day you are printing on (each)
SlideMate?

 

Thanks,

 

Sandy C. Harrison, HTL (ASCP)

Histology Supervisor

Minneapolis VA

612-467-2449

 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


RE: [Histonet] RE: Metal molds

2012-10-10 Thread joelle weaver

My bad, I guess I misunderstood. I still like them clean, even if it seems 
unecessary.




Joelle Weaver MAOM, HTL (ASCP) QIHC
  Subject: RE: [Histonet] RE: Metal molds
 Date: Wed, 10 Oct 2012 12:18:48 -0600
 From: billodonn...@catholichealth.net
 To: joellewea...@hotmail.com; brendal.fin...@medicalcenterclinic.com; 
 histonet@lists.utsouthwestern.edu
 
 Actually, the thread began because our VIP rotary valve got filled with
 wax and required service. We clean daily in VIP, but wanted to know what
 others were doing. Mostly, people are using the VIP, so we may need to
 modify our regularity or change out cleaning solution more frequently.
 
 I do like where the thread has gone. I got my (chicken fryer) cast iron
 skillet from my mother who got it from her mother. They were migrant
 workers during the dust bowl,  picking cotton in Texas and oranges in
 California before settling in Arizona. I remember Mom frying chicken (or
 the occasional rabbit) in it on Sunday mornings. Yes, I still use it,
 but I have added several other cast iron pieces to the arsenal including
 a bread pan! There will be pound cake in the oven tonight!
 
 William (Bill) O'Donnell, HT (ASCP) QIHC 
 Senior Histologist
 Good Samaritan Hospital
 10 East 31st Street
 Kearney, NE 68847 
 
 SERENITY is not freedom from the storm, but peace amid the storm.
 
 Cultivate it in PRAYER!
 
  
 
 
 
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle
 weaver
 Sent: Wednesday, October 10, 2012 12:42 PM
 To: brendal.fin...@medicalcenterclinic.com;
 histonet@lists.utsouthwestern.edu
 Subject: RE: [Histonet] RE: Metal molds
 
 
 My impression of the intial thread was the general cleanliness and cross
 contamination potential, not the fact that the blocks aren't easily
 released. I have never had that issue. 
 
 
 
 
 Joelle Weaver MAOM, HTL (ASCP) QIHC
   Date: Wed, 10 Oct 2012 09:27:09 -0500
  From: brendal.fin...@medicalcenterclinic.com
  To: histonet@lists.utsouthwestern.edu
  Subject: RE: [Histonet] RE: Metal molds
  
  
  We clean molds every day.  My preferred method is heated water to melt
 
  the paraffin off, then allow to cool.  Peel the paraffin from the 
  surface of the water, remove the molds from the water, then dunk them 
  about 10 times in a mixture of alcohol and mold release.  Allow to air
 
  dry or dry in a low temp oven.
  
  
  In my experience, molds that aren't cleaned on a regular basis make it
 
  very difficult to remove the embedded cassettes even if very, very 
  cold.  It's easier for me if my workspace and tools are clean and 
  organized.
  
  
  -Original message-
  From: susan.wal...@hcahealthcare.com
  Date: Wed, 10 Oct 2012 01:00:50 -0500
  To: joellewea...@hotmail.com, valerie.han...@parrishmed.com
  Subject: RE: [Histonet] RE: Metal molds
  
   We put our molds in the VIP before running the cleaning cycle daily.
  Then we dip them in alcohol containing mold release..air dry and 
  store.
   
   -Original Message-
  From: histonet-boun...@lists.utsouthwestern.edu
  [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle
 
  weaver
   Sent: Tuesday, October 09, 2012 3:27 PM
   To: valerie.han...@parrishmed.com
   Cc: histonet@lists.utsouthwestern.edu
   Subject: RE: [Histonet] RE: Metal molds
   
   
   I always cleaned them daily, either the very hot water, soapy water
  method, with water running over them in the sink with them on their 
  sides so it passes over them, not upright so the water sits in them- 
  then a rinse in alcohol and completely air dry. Or you can always do 
  the clean cycle with the racks, running them through xylene, etc. They
 
  come out very clean this way- used an old processor that was a backup 
  for this most of the time. But I always did them daily, but also wiped
 
  each one out with gauze if I used them twice in an embedding session (
 
  for more than one specimen in that large batch). Also I like metal, I 
  hate those plasticones. If you keep the block face surface of the mold
 
  warm-hot, and flatten before it turns completely white the specimen is
 
  at the surface and you are able to see the edges easily without a lot 
  of facing. I think this saves time cutting through paraffin, and 
  saves blades. Plus if the specimen is not flat enough, you see it 
  right away and know if you must re-embed to get a complete, 
  representative section, rather than after you have cut some 
  superficial parts of some edges away and not others, only to have to 
  re-embed anyhow. The other problems I see are when people are afraid
  of big molds- please if you are only taking one section, use one large
 
  enough to leave a perimeter. Don't try to squeeze it into a medium 
  mold, you are unlikely to need multiple sections on one slide and it 
  is much easier to get flat and get a good section. Also please put 
  enough paraffin on top, so that when it 

RE: [Histonet] Are those sites using the Thermo Fisher Slide Mate slide printer also using Thermo slides?

2012-10-10 Thread Elizabeth Chlipala
Sandra

We had one slide mate that we purchased few years back, we had problems from 
the beginning with both fisher and other slides, right now the machine is 
sitting in a cabinet not being used.  To me we found it very unreliable with 
respects to print quality.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, 
Sandra C.
Sent: Wednesday, October 10, 2012 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Are those sites using the Thermo Fisher Slide Mate slide 
printer also using Thermo slides?

I just purchased 6 Slide Mates.  We just began using them yesterday.



Are any of you using slides that are NOT Fisher slides on your
SlideMates?



When you respond, could you answer the following questions?

a)  Is the print job consistently satisfactory?

b)  How long have had your SlideMate(s)?

c)   What difficulties did you have, getting it to consistently
print well?

d)  Approximately how many slides per day you are printing on (each)
SlideMate?



Thanks,



Sandy C. Harrison, HTL (ASCP)

Histology Supervisor

Minneapolis VA

612-467-2449



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[Histonet] FW: HT/HTL PROGRAM DIRECTORS SURVEY

2012-10-10 Thread Jesus Ellin
Please read this and I would like to know what peoples thoughts are about this 
issue.  Also take into consideration where we are head and also testing.  This 
is extremely interesting.


From: Robert Newberry
Sent: Wednesday, October 10, 2012 3:39 PM
To: Jesus Ellin
Subject: FW: HT/HTL PROGRAM DIRECTORS SURVEY
Importance: High


FYI

From: Julie Stiak [mailto:julie.st...@phoenixcollege.edu]
Sent: Tuesday, October 09, 2012 11:12 AM
To: algra...@email.arizona.edu; ghurf...@carondelet.org; jfille...@shc.org; 
joyce.h...@bannerhealth.com; lhi...@cgrmc.org; michelle.man...@mihs.gov; Amy 
Wendel Spiczka; Andy Burnette; Aprill Watanabe; Arthur Sitelman; 
barbara.blasutta; Bill Miller; Bob Wenham; Brian Stillwell; Dana Lake; Donna 
Vollmer; Ethel Macrea; Gary; Gwin Filleman; Holly Hanson-Kollar; Ida Male; 
Irene Campbell; Janani Siva; Jodi Evers-Dewald; Joe Ferreira; Joseph Berryhill; 
Karen Lahti; Kathy Hill-Epperson; Meredith Hale; Nicol Wargocz; Scott Cockayne; 
Stacie Schimke; Susan Pitts; Sutter, Katarina; mary.ac...@bannerhealth.com; 
patricia.tom...@bannerhealth.com; rr...@phoenixchildrens.com; Amy Spinti; 
Robert Newberry; Brigitte.Miedzybrocki; Cary Nava; Chelle Johnson; Cheryl 
Mossing; Chris Garza; Chris Westhoff; dan.otis; Diana McGregor; Eadie Baie; 
Elaine Fought; Fabrizio Saraceni; Georgia Koehler; Hans Baijense; Holland, 
Libby; James Taylor; Jeff Wolz; Jimmie Evans; joyce.santis; Judy Davis; Julie 
Pilkington; Kathryn Wangsness; Kathy Knight; Laura Bell; leslie.lyford; Liz 
Beauford; Lori Watkins; Marie Holub; Mark Wooden; Maya Sinha; McMillan, Susan - 
SJHMC; Merrikay Vidal; Mimi Wettach; Nancy Behling; Pamela Green; Pattie Glick; 
Peter Michaelson; Phil Hynes; Rachel Baker; rick.ro...@bannerhealth.com; Robert 
Graham; Rosemarie Prater; Ruth Spates; Salene Slader; Sally Caglioti; Shannon 
Sadat-Abhary; Sherry Gamble; Sponsler, Pam; Suzanne Sullivan; Tajuan Hamilton; 
Terri Bowen; Tim Hersom; Tony Millett; Valerie Kolody; Victor Waddell; Ward, 
Marilyn
Subject: Fwd: HT/HTL PROGRAM DIRECTORS SURVEY

This survey is very TIMELY regarding ASCP's potential interest in resurrecting 
the Clinical Lab Assistant national certification and to expand the role to 
basic embedding and processing in Histology!

The timing is interesting for Phoenix College as we had record number of 
applicants for the current Histologic Technology and future Medical Laboratory 
Science cohorts,  BUT just decided late last week to CANCEL the FALL Laboratory 
Assisting program as we only had 6 students.

I am trying to garner administrative approval to facilitate an industry 
sponsored partnership to provide the fall cohort to a few local Lab employees 
to be able to complete the program via distance learning this fall.

The topic of a Clinical Lab Assistant will be added to the fall HT Advisory 
Council meeting and the spring Medical Laboratory Science Advisory Council 
meeting.

Please feel free to share your thoughts with me before the meeting about the 
potential expansion of a nationally certified Clinical Lab Assistant.

Many thanks!

Julie

-- Forwarded message --
From: Peggy Wenk pw...@beaumont.edumailto:pw...@beaumont.edu
Date: Tue, Oct 9, 2012 at 8:36 AM
Subject: HT/HTL PROGRAM DIRECTORS SURVEY
To: \car...@nsh.orgmailto:car...@nsh.org\.GWIA.MSD 
car...@nsh.orgmailto:car...@nsh.org, Aulthouse, Amy 
a-aultho...@onu.edumailto:a-aultho...@onu.edu, Bailey, Mark 
mabai...@mdanderson.orgmailto:mabai...@mdanderson.org, Barone, Carol 
cbar...@nemours.orgmailto:cbar...@nemours.org, Beamon, Nancy 
nancy.bea...@darton.edumailto:nancy.bea...@darton.edu, Becker, Carol 
carol.e.bec...@osfhealthcare.orgmailto:carol.e.bec...@osfhealthcare.org, 
Bischof, Carol 
carol.bisc...@minnesota.edumailto:carol.bisc...@minnesota.edu, Campagna, 
Gerard gcam...@conemaugh.orgmailto:gcam...@conemaugh.org, Christopher 
Mignogna 
(christopher.migno...@drexel.edumailto:christopher.migno...@drexel.edu) 
christopher.migno...@drexel.edumailto:christopher.migno...@drexel.edu, 
Colony, Pamela colo...@cobleskill.edumailto:colo...@cobleskill.edu, 
Counts, Lisa lisa...@yahoo.commailto:lisa...@yahoo.com, Cox, Beth 
bethc...@gmail.commailto:bethc...@gmail.com, Davidson, Kelli 
jreyno...@mail.accd.edumailto:jreyno...@mail.accd.edu, Della Speranza, 
Vincent del...@musc.edumailto:del...@musc.edu, Delost, Maria 
medel...@ysu.edumailto:medel...@ysu.edu, DiNardo, Helen 
helen.dina...@shermanhospital.orgmailto:helen.dina...@shermanhospital.org, 
Donna Broderick (dbroder...@harcum.edumailto:dbroder...@harcum.edu) 
dbroder...@harcum.edumailto:dbroder...@harcum.edu, Durkin, Zoe Ann 
zdur...@goodwin.edumailto:zdur...@goodwin.edu, Feaster, Kimberly 
kfeas...@hsc.wvu.edumailto:kfeas...@hsc.wvu.edu, 
lfoster-br...@christianacare.orgmailto:lfoster-br...@christianacare.org 
lfoster-br...@christianacare.orgmailto:lfoster-br...@christianacare.org, 
Galina Negrouk 

[Histonet] Tucson HT Needed

2012-10-10 Thread Hale, Meredith
Great full time  opportunities' for a Histotechnician in Tucson, Arizona! Adobe 
Gastroenterology   is looking for certified HT or HTL and a Laboratory 
Assistant to join their new laboratory . HT candidate must meet the following 
criteria:

* Meet CLIA Grossing Requirements : CFR  493.1489,  
http://wwwn.cdc.gov/clia/regs/toc.aspx/ ,prior experience grossing GI specimens

* HT ASCP Certified
Duties include:

* Grossing

* Embedding

* Microtomy

* Staining

* Ability to be flexible and take on additional duties' as needed


These positions offer a competitive rate and flexible hours.  Interested 
applicants should contact Meredith Hale; phone 214-596-2219 or through email 
mh...@miracals.commailto:mh...@miracals.com


Meredith Hale HT  (ASCP)cm
Operations Liaision Director and Education Coordinator

Miraca Life Sciences
6655 North MacArthur Blvd.
Irving , Texas 75039
Office: 214-596-2219
Cell: 469-648-8253
Fax: 1-866-688-3280
mh...@miracals.commailto:mh...@miracals.com

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RE: [Histonet] Are those sites using the Thermo Fisher Slide Mate slide printer also using Thermo slides?

2012-10-10 Thread Ian R Bernard
We have also acquired a Thermo Fisher slide mate/micro writer in Mar/Apr of 
this year. We have yet to have them work and consistently.  The printing 
quality despite the type slides used is inconsistent.  Nevertheless, I am 
giving them opportunity to fix it since we have invested money.

IB
USAFA, Colorado Springs.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth 
Chlipala
Sent: Wednesday, October 10, 2012 2:12 PM
To: 'Harrison, Sandra C.'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Are those sites using the Thermo Fisher Slide Mate 
slide printer also using Thermo slides?

Sandra

We had one slide mate that we purchased few years back, we had problems from 
the beginning with both fisher and other slides, right now the machine is 
sitting in a cabinet not being used.  To me we found it very unreliable with 
respects to print quality.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 
18592 Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, 
Sandra C.
Sent: Wednesday, October 10, 2012 1:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Are those sites using the Thermo Fisher Slide Mate slide 
printer also using Thermo slides?

I just purchased 6 Slide Mates.  We just began using them yesterday.



Are any of you using slides that are NOT Fisher slides on your SlideMates?



When you respond, could you answer the following questions?

a)  Is the print job consistently satisfactory?

b)  How long have had your SlideMate(s)?

c)   What difficulties did you have, getting it to consistently
print well?

d)  Approximately how many slides per day you are printing on (each)
SlideMate?



Thanks,



Sandy C. Harrison, HTL (ASCP)

Histology Supervisor

Minneapolis VA

612-467-2449



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