[Histonet] looking for two speakers from NSH Vancouver
I was wondering if anyone new the names of and how to get in touch with the two speakers that gave the 8:00 am lecture Saturday September 29th titled Getting Good Sections of Anything? If anybody knows out there in histo land and can help I would greatly appreciate it :) Nancy Heath, HT (ASCP) Neuropathology Tech Specialist Dept. of Pathology., Div. of Neuropathology Rhode Island Hospital APC Blding, Flr 12, Rm 211 593 Eddy Street Providence, RI 02903 lab: 401-444-3246 fax: 401-444-9889 nhe...@lifespan.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: looking for two speakers from NSH Vancouver
The instructors were Curt King and Jen Freeland Best reagards, Nanne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Heath, Nancy L. Sent: Tuesday, November 13, 2012 7:40 AM To: Histonet Subject: [Histonet] looking for two speakers from NSH Vancouver I was wondering if anyone new the names of and how to get in touch with the two speakers that gave the 8:00 am lecture Saturday September 29th titled Getting Good Sections of Anything? If anybody knows out there in histo land and can help I would greatly appreciate it :) Nancy Heath, HT (ASCP) Neuropathology Tech Specialist Dept. of Pathology., Div. of Neuropathology Rhode Island Hospital APC Blding, Flr 12, Rm 211 593 Eddy Street Providence, RI 02903 lab: 401-444-3246 fax: 401-444-9889 nhe...@lifespan.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] staffing numbers
Hi Everyone, I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs. Any information will be appreciated Thank you Allison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DAPI turning orange
Has anyone had a problem with DAPI looking beautifully blue nuclear staining and then have it orange the next day? What is the problem and the cure? Thank you Beth Millerman, Stiefel Labs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
Nope, sorry. All your fat is dissolved. Sent from my iPhone On Nov 13, 2012, at 8:52 AM, z o n k e d zon...@gmail.com wrote: Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
Oil Red O is a stain for fat, Alcohol dissolves fat Rehydrating won't help. You can't replace the fat. Rena Fail On Tue, Nov 13, 2012 at 11:52 AM, z o n k e d zon...@gmail.com wrote: Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
Ah, I knew it. :( Thank you. Zoe On Tuesday, November 13, 2012, Will Chappell wrote: Nope, sorry. All your fat is dissolved. Sent from my iPhone On Nov 13, 2012, at 8:52 AM, z o n k e d zon...@gmail.com javascript:; wrote: Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu javascript:; http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
It depends on what you are using the oil red o for. Lipofuscin and ceroid can be demonstrated with an oil red o stain after processing. Jennifer MacDonald From: z o n k e d zon...@gmail.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: 11/13/2012 08:53 AM Subject:[Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! Sent by:histonet-boun...@lists.utsouthwestern.edu Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Xpress Question
We are going to go w the Xpress 120. I have worked with it before, but stuff changes. What are Xpress users on histonet doing to clean the baskets and handles after use? We formerly used a dishwasher, but that is not a favorite of ouor plumbing folks fearing that we will clog drains w wax. I guess they think we are going to be pouring gallons of molten wax through the system. Any help would be great help! -Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Problem with cardiomyocytes staining
Dear Histonetters, I am new in histology so I ask if someone have experience with cardiac tissue. Recently I performed a Picrosirius Red staining (pig atrium, 5 microns thickness) but in some sections I noted cardiomyocytes colored brown (instead of pale yellow).I wonder if it is probably due to the thickness of the section or something alse(unfortunately I use an old microtome whose probably fails to work in the correct way). Thank you in advance Best regards Laura Laura Avogaro Center for Biomedical Technologies (BIOTECH) University of Trento Italy ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Xpress Question
We have a great, small, portable dishwasher that we use to wash the baskets as well as our coplin jars. Just make sure you wipe all the molten paraffin off the baskets thoroughly before you wash them. We have had no problems at all re: plumbing. I can send info. re: the dishwasher. At the time we bought it it was under $200.00 and has been a great workhouse. It's not even a lab dishwasher! Jeanine H. Bartlett Centers for Disease Control and Prevention Infectious Diseases Pathology Branch 404-639-3590 jeanine.bartl...@cdc.hhs.gov -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill Sent: Tuesday, November 13, 2012 12:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xpress Question We are going to go w the Xpress 120. I have worked with it before, but stuff changes. What are Xpress users on histonet doing to clean the baskets and handles after use? We formerly used a dishwasher, but that is not a favorite of ouor plumbing folks fearing that we will clog drains w wax. I guess they think we are going to be pouring gallons of molten wax through the system. Any help would be great help! -Bill William (Bill) O'Donnell, HT (ASCP) QIHC Senior Histologist Good Samaritan Hospital 10 East 31st Street Kearney, NE 68847 SERENITY is not freedom from the storm, but peace amid the storm. Cultivate it in PRAYER! This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
We just wanted to see general lipids, nothing in particular. This mouse
died unexpectedly and may have been part of a group that was put on a high
fat or high bile acid diet and we just wanted to see what happened.
On Tuesday, November 13, 2012, Jennifer MacDonald wrote:
It depends on what you are using the oil red o for. Lipofuscin and ceroid
can be demonstrated with an oil red o stain after processing.
Jennifer MacDonald
From:z o n k e d zon...@gmail.com javascript:_e({}, 'cvml',
'zon...@gmail.com');
To:histonet@lists.utsouthwestern.edu javascript:_e({}, 'cvml',
'histonet@lists.utsouthwestern.edu');
histonet@lists.utsouthwestern.edujavascript:_e({}, 'cvml',
'histonet@lists.utsouthwestern.edu');
Date:11/13/2012 08:53 AM
Subject:[Histonet] Help! Liver mistakenly processed in paraffin
(had to bein OCT instead)!
Sent by:histonet-boun...@lists.utsouthwestern.edujavascript:_e({},
'cvml', 'histonet-boun...@lists.utsouthwestern.edu');
--
Hello Histonetters,
First time writer, long time reader. I'm a newbie tech in academia and I
was given a simple task which I think I pretty much screwed up.
I should have embedded half of a mouse liver in paraffin for microtome
sectioning while the other half should have been embedded in OCT for
cryosectioning (for oil red o). I made the mistake last night of placing
both liver halves into the tissue processor. The liver I intended for OCT
embedding is now hard as wax. Is there any way to deparaffinize processed
organs and may I embed them in OCT for proper cryosectioning? I imagine
that the liver would get dehydrated, I would get crappy sections, and Oil
Red O won't work.
Any suggestions are welcome.
Thank you so much,
Zoe W.
--
It costs nothing to say something kind. Even less to shut up altogether.
--Nathan Fillion
___
Histonet mailing list
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'Histonet@lists.utsouthwestern.edu');
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--
It costs nothing to say something kind. Even less to shut up altogether.
--Nathan Fillion
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] On call
This question may be a bit off subject Histonet, but I have not been able to find information on this subject. Our facility has decided to pay our Pathologist for their on call duties. I have been tasked with finding what other facilities are paying for this service. Any information would be appreciated. Thank You Gary ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: staffing numbers
We process ~14.0K cases/yr and 34.5K blocks/yr. We have 4 techs with me working part time on the bench. Lynette Lynette Pavelich, HT(ASCP) Histology Supervisor Hurley Medical Center One Hurley Plaza Flint, MI 48503 ph: 810.262.9948 mobile: 810.444.7966 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Hutton, Allison [ahut...@dh.org] Sent: Tuesday, November 13, 2012 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs. Any information will be appreciated Thank you Allison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
if there was fat replacement, such as cirrhosis, you will see it in the morphology. From: z o n k e d zon...@gmail.com To: Jennifer MacDonald jmacdon...@mtsac.edu Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu Date: 11/13/2012 09:43 AM Subject:Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! We just wanted to see general lipids, nothing in particular. This mouse died unexpectedly and may have been part of a group that was put on a high fat or high bile acid diet and we just wanted to see what happened. On Tuesday, November 13, 2012, Jennifer MacDonald wrote: It depends on what you are using the oil red o for. Lipofuscin and ceroid can be demonstrated with an oil red o stain after processing. Jennifer MacDonald From:z o n k e d zon...@gmail.com To:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date:11/13/2012 08:53 AM Subject:[Histonet] Help! Liver mistakenly processed in paraffin (had to bein OCT instead)! Sent by:histonet-boun...@lists.utsouthwestern.edu Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
Thank you all for your helpful responses! I will go ahead and carry on with paraffin sectioning and stain with HE as planned. As for the Oil Red O, I'll try that too and see what happens. (Still amazed at how amazing Histonet is and how helpful you all are! Thank you very much for all your responses!) On Tue, Nov 13, 2012 at 2:01 PM, Jennifer MacDonald jmacdon...@mtsac.eduwrote: if there was fat replacement, such as cirrhosis, you will see it in the morphology. From:z o n k e d zon...@gmail.com To:Jennifer MacDonald jmacdon...@mtsac.edu Cc:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu, histonet-boun...@lists.utsouthwestern.edu histonet-boun...@lists.utsouthwestern.edu Date:11/13/2012 09:43 AM Subject:Re: Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! -- We just wanted to see general lipids, nothing in particular. This mouse died unexpectedly and may have been part of a group that was put on a high fat or high bile acid diet and we just wanted to see what happened. On Tuesday, November 13, 2012, Jennifer MacDonald wrote: It depends on what you are using the oil red o for. Lipofuscin and ceroid can be demonstrated with an oil red o stain after processing. Jennifer MacDonald From:z o n k e d *zon...@gmail.com* To:*histonet@lists.utsouthwestern.edu* * histonet@lists.utsouthwestern.edu* Date:11/13/2012 08:53 AM Subject:[Histonet] Help! Liver mistakenly processed in paraffin (had to bein OCT instead)! Sent by:*histonet-boun...@lists.utsouthwestern.edu* -- Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list* **Histonet@lists.utsouthwestern.edu** **http://lists.utsouthwestern.edu/mailman/listinfo/histonet*http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] staffing numbers
Allison: The information you need is in at http://histosearch.com/rene.html René J. From: Hutton, Allison ahut...@dh.org To: histonet@lists.utsouthwestern.edu Sent: Tuesday, November 13, 2012 11:00 AM Subject: [Histonet] staffing numbers Hi Everyone, I am looking for some crude numbers regarding staffing. I would like to know the number of cases done per year and your number of histotechs. Any information will be appreciated Thank you Allison ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!
You really screwed it up! When you placed both pieces of liver in the processor both were subjected to the effect of ethanol and probably xylene and both reagents extracted the liver fat and no matter what you try to do now, there will be not enough fat in the pieces as to even try the ORO stain. Try to get another piece. Anything you will try will not render good cryosections and no fat staining. René J. From: z o n k e d zon...@gmail.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, November 13, 2012 11:52 AM Subject: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)! Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I should have embedded half of a mouse liver in paraffin for microtome sectioning while the other half should have been embedded in OCT for cryosectioning (for oil red o). I made the mistake last night of placing both liver halves into the tissue processor. The liver I intended for OCT embedding is now hard as wax. Is there any way to deparaffinize processed organs and may I embed them in OCT for proper cryosectioning? I imagine that the liver would get dehydrated, I would get crappy sections, and Oil Red O won't work. Any suggestions are welcome. Thank you so much, Zoe W. -- It costs nothing to say something kind. Even less to shut up altogether. --Nathan Fillion ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] liftin tissue
I need to lift and transfer one stained section from a slide and transfer it to another slide to destain the HE and run an immuno on it. Does anyone have a procedure for doing that? Thank you. Lee The materials and information in this e-mail are confidential and may contain Protected Health Information covered under the HIPAA Privacy Rule. If you are not the intended recipient, be advised that any unauthorized use, disclosure, copying, distribution, or action taken in reliance on the contents of this information is strictly forbidden by law. If you have received this e-mail in error, please notify me by reply e-mail and then delete this message. Do not pass any of this information to anyone else. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Problem with cardiomyocytes staining
Hi Laura, I do this stain very frequently. It can be finickey. You should make very sure the pH is below 2.5. It could be that you have an old solution and over time these tend to drift toward a neutral pH. This will affect the staining. Sometimes the solutions need to be discarded and either re-made or have new purchased. Best of luck, Amos On Tue, Nov 13, 2012 at 1:00 PM, histonet-requ...@lists.utsouthwestern.eduwrote: Message: 18 Date: Tue, 13 Nov 2012 18:32:09 +0100 (CET) From: Laura Avogaro avog...@science.unitn.it Subject: [Histonet] Problem with cardiomyocytes staining To: histonet@lists.utsouthwestern.edu Message-ID: 3064.192.168.178.78.1352827929.squir...@www.science.unitn.it Content-Type: text/plain;charset=iso-8859-1 Dear Histonetters, I am new in histology so I ask if someone have experience with cardiac tissue. Recently I performed a Picrosirius Red staining (pig atrium, 5 microns thickness) but in some sections I noted cardiomyocytes colored brown (instead of pale yellow).I wonder if it is probably due to the thickness of the section or something alse(unfortunately I use an old microtome whose probably fails to work in the correct way). Thank you in advance Best regards Laura ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Cassette and slide labelling systems
Hi Everyone:I'm looking for opinions on cassette and slide labelling systems. Please share your likes and dislikes.ThanksSheila p.s Vendors Do Not call me over this email. That's very annoying ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
