[Histonet] Processing Protocol Using Mineral Oil
We have been using this protocol on mostly mouse tissues with great results! We run this on a VIP 3000. 70% Ethanol, room temp, 1 hour 100% Isopropyl Alcohol, room temp, 1 hour 100% Isopropyl Alcohol, room temp, 1 hour 100% Isopropyl Alcohol, room temp, 1 hour 5:1 Iso:Mineral Oil, 50 degrees, 1 hour 2:1 Iso:Mineral Oil, 50 degrees, 1 hour 100% Mineral Oil, 50 degrees, 1 hour 100% Mineral Oil, 50 degrees, 1 hour 100% Mineral Oil, 50 degrees, 6 hours Paraffin, 60 degrees, 1 hour Paraffin, 60 degrees, 1 hour Paraffin, 60 degrees, 1 hour Followed by a cleaning cycle after cassettes are removed and put into molten paraffin at the embedding center Lyn M. Stadler, BS, HTL(ASCP)CM Research Histotechnologist Department of Histopathology Cleveland Biolabs, Inc. 73 High Street Buffalo, NY 14203 716-849-6817, ext 417 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, December 05, 2012 1:05 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 109, Issue 6 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: automated microtomes (Lynette Pavelich) 2. HistoTALK (David Kemler) 3. RE: Tissue Processors (Marcum, Pamela A) 4. RE: Tissue Processors (Boyd, Debbie M) 5. Re: Immunohistochemical detection of cytokines in older frozen sections (Rob Day) 6. RE: Histonet Digest, Vol 109, Issue 5 (Riesen, Rebecca) 7. Re: Fixation time (Geoff) 8. Histology Positions in Little Rock AR (Marcum, Pamela A) 9. Re: Fixation time (Teri Johnson) 10. Re: Tissue Processors (Teri Johnson) 11. job opening in PA (Hutton, Allison) 12. Re: Re: Tissue Processors (Rene J Buesa) 13. RE: Re: Tissue Processors (Ellenburg, Deborah) -- Message: 1 Date: Wed, 5 Dec 2012 12:10:06 + From: Lynette Pavelich lpave...@hurleymc.com Subject: RE: [Histonet] automated microtomes To: Rene J Buesa rjbu...@yahoo.com, Rathborne, Toni trathbo...@somerset-healthcare.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 89f4666a496dc949a819ecc40e11c867bf56c...@exchangemb1.hmc.hurleymc.com Content-Type: text/plain; charset=iso-8859-1 I am purchasing my second Leica RM2255 automated microtome. Rene' is correct in saying that the carpal tunnel syndrome will eventually affect every advanced tech by using any microtome manually. On the RM2255, the flywheel additionally is automated. You can choose to use the flywheel manually or automated by the simultaneous pushing of two buttons. Nice feature for us oldies to ease us into automation or if you have a tiny specimen that you need to take extra care with and want to use manually. Companies are very happy to send in a demo to try for a couple weeks. Happy shopping!! ;) Lynette From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Rene J Buesa [rjbu...@yahoo.com] Sent: Tuesday, December 04, 2012 1:19 PM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] automated microtomes The advantage of the so called automated microtomes (the only thing automated about them is the block advance) is that they alleviate wrist effort and in some ways prevent carpal tunnel syndrome that affects some histotechs (mostly of the senior persuasion). I would go with the Leica. Ren? J. From: Rathborne, Toni trathbo...@somerset-healthcare.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, December 4, 2012 11:48 AM Subject: [Histonet] automated microtomes I'm looking for some opinions about the automated microtomes currently available. Which ones do most techs prefer? Which are more reliable? Is there an advantage to having a semi-automated microtome? Thanks in advance for your replies. Toni ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 2 Date: Wed, 5 Dec 2012 04:28:54 -0800 (PST) From: David Kemler histot...@yahoo.com
RE: [Histonet] Disposal of DAB-link to Princeton.
Please correct me if I am wrong but if you use potassium permanganate sulfuric acid, do you not deactivate the mutagenic properties? Then disposal would not require a hazardous waste container. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewal...@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition® and the Governor's Award for Performance Excellence -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Monday, December 03, 2012 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposal of DAB-link to Princeton. DAB is a mutagen and potential carcinogen. Waste needs to be disposed of as hazardous chemical waste. http://web.princeton.edu/sites/ehs/chemwaste/DAB.htm Jerry From: cha...@yahoo.com Date: Wed, 28 Nov 2012 13:14:26 -0800 To: cp...@x-celllab.com Subject: Re: [Histonet] chemical disposal CC: histonet@lists.utsouthwestern.edu Chemical hazardous waste. Sent from my iPhone On Nov 28, 2012, at 12:44 PM, Cynthia Pyse cp...@x-celllab.com wrote: Quick question for Histoland. I am having a debate about DAB disposal. Our general manager ( non lab background) insists that the liquid DAB can go into a biological hazardous waste. I disagree, it is a chemical and needs to be disposed in the chemical hazardous waste. What is everyone else doing to dispose of DAB. We are located in NY, I do have those regs. Thanks in advance for any and all help. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Disposal of DAB-link to Princeton.
That's exactly what we do. After the potassium permanganate /sulfuric acid we neutralize it and put in down the drain with copious amounts of water. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hor...@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Thursday, December 06, 2012 10:45 AM To: Jerry Ricks; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Disposal of DAB-link to Princeton. Please correct me if I am wrong but if you use potassium permanganate sulfuric acid, do you not deactivate the mutagenic properties? Then disposal would not require a hazardous waste container. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewal...@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Monday, December 03, 2012 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposal of DAB-link to Princeton. DAB is a mutagen and potential carcinogen. Waste needs to be disposed of as hazardous chemical waste. http://web.princeton.edu/sites/ehs/chemwaste/DAB.htm Jerry From: cha...@yahoo.com Date: Wed, 28 Nov 2012 13:14:26 -0800 To: cp...@x-celllab.com Subject: Re: [Histonet] chemical disposal CC: histonet@lists.utsouthwestern.edu Chemical hazardous waste. Sent from my iPhone On Nov 28, 2012, at 12:44 PM, Cynthia Pyse cp...@x-celllab.com wrote: Quick question for Histoland. I am having a debate about DAB disposal. Our general manager ( non lab background) insists that the liquid DAB can go into a biological hazardous waste. I disagree, it is a chemical and needs to be disposed in the chemical hazardous waste. What is everyone else doing to dispose of DAB. We are located in NY, I do have those regs. Thanks in advance for any and all help. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by e-mail. Thank You ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of
RE: [Histonet] Disposal of DAB-link to Princeton.
Everyone in histology should get a copy of Anatech's book Hazardous Waste In the Histopathology Laboratory. It goes through all this in detail. http://www.anatechltdusa.com/Hazmat.html Catalog # 051 $88.40 Hazmat Manual New Features Hardbound Fully indexed Convenient 6 x 9 size Comprehensive revisions throughout, including... updated references and resources extensive list of contacts with web addresses latest recommendations from standard-setting organizations new chapter on ASCP/BOR Specialist in Laboratory Safety certification exam Order your copy now! Call 1.800.262.8324 Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical Center -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V Sent: Thursday, December 06, 2012 8:51 AM To: 'Joe W. Walker, Jr.'; Jerry Ricks; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Disposal of DAB-link to Princeton. That's exactly what we do. After the potassium permanganate /sulfuric acid we neutralize it and put in down the drain with copious amounts of water. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax hor...@archildrens.org archildrens.org 100 YEARS YOUNG! JOIN THE PARTY AT ach100.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe W. Walker, Jr. Sent: Thursday, December 06, 2012 10:45 AM To: Jerry Ricks; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Disposal of DAB-link to Princeton. Please correct me if I am wrong but if you use potassium permanganate sulfuric acid, do you not deactivate the mutagenic properties? Then disposal would not require a hazardous waste container. Joe W. Walker, Jr. MS, SCT(ASCP)CM Anatomical Pathology Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P: 802.747.1790 F: 802.747.6525 NEW EMAIL: joewal...@rrmc.org www.rrmc.org Our Vision: To be the Best Community Healthcare System in New England Rutland Regional...Vermont's 1st Hospital to Achieve Both ANCC Magnet Recognition(r) and the Governor's Award for Performance Excellence -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jerry Ricks Sent: Monday, December 03, 2012 2:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposal of DAB-link to Princeton. DAB is a mutagen and potential carcinogen. Waste needs to be disposed of as hazardous chemical waste. http://web.princeton.edu/sites/ehs/chemwaste/DAB.htm Jerry From: cha...@yahoo.com Date: Wed, 28 Nov 2012 13:14:26 -0800 To: cp...@x-celllab.com Subject: Re: [Histonet] chemical disposal CC: histonet@lists.utsouthwestern.edu Chemical hazardous waste. Sent from my iPhone On Nov 28, 2012, at 12:44 PM, Cynthia Pyse cp...@x-celllab.com wrote: Quick question for Histoland. I am having a debate about DAB disposal. Our general manager ( non lab background) insists that the liquid DAB can go into a biological hazardous waste. I disagree, it is a chemical and needs to be disposed in the chemical hazardous waste. What is everyone else doing to dispose of DAB. We are located in NY, I do have those regs. Thanks in advance for any and all help. Cindy Cindy Pyse, CLT, HT (ASCP) Laboratory Manager X-Cell Laboratories 20 Northpointe Parkway Suite 100 Amherst, NY 14228 716-250-9235 etx. 232 e-mail cp...@x-celllab.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message (and any included attachments) is from Rutland Regional Health Services and is intended only for the addressee(s). The information contained herein may include privileged or otherwise confidential information. Unauthorized review, forwarding, printing, copying, distributing, or using such information is strictly prohibited and may be unlawful. If you received this message in error, or have reason to believe you are not authorized to receive it, please promptly delete this message and notify the sender by
[Histonet] How to open Reichert 820?
Hi Anyone know how to open the cover of a Reichert 820 microtome? This is the model that has a wheel for the coarse advance on the left side. I am used to the AO Style, pop the latch tip it back. These usually have a crank and a cut out slot on the left. This one uses 4 screws from the bottom to secure the lid and there is no cut out for the coarse advance to slide through. Looks like the wheel has to be removed to remove the cover. Getting the wheel off is where I am stuck. This is an old microtome we have had sitting around, would like to check the guts and clean it up. Jon Jonathan Krupp Applied Science, Business Technology San Joaquin Delta College 5151 Pacific Ave. Stockton, CA 95207 209-954-5284 jkr...@deltacollege.edu Find us on Facebook @ Electron Microscopy at SJ Delta College ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] DAKO Her2
Hi All, sorry for how I am submitting this but I have been sending in questions to the address provided by nothing is going through. I would like to know who uses DAKO's IVD Her2 IHC marker and how it works. We want to start running this on our Leica BOND and any info would be much appreciated. Thanks Joanne Clark, HT(ASCP) Histology Supervisor Pathology Consultants of New Mexico Roswell, NM ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Paraformaldehyde Solution Recipe for Perfusion
Hi all, We are performing transcardial perfusions in rats using paraformaldehyde in 0.1M potassium phosphate buffer. Can anyone think of any issues that would be caused by using phosphate buffer made from solely potassium salts (basic and dibasic), rather than a mixture of sodium and potassium or only the sodium salts? We do our rinse with 0.1 M PB + Saline and then follow up with solutions just made up in the potassium phosphate buffer (therefore no Na+). Could this cause any tonicity/osmolarity issues? We are trying to troubleshoot some issues we are having with the perfused tissue. Thanks! - Andrew ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Embedding Centers
Both animal disease labs in the state of Illinois have used the Sakura machines for the twenty years I have been here with great success. We also have aSakura processor that has been going for 25 years and a coverslipper that has only had 3 service calls for minor problems in the past 15+ years. They make good products. Lynn Burton Animal Disease Lab Galesburg, Il -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Thursday, December 06, 2012 2:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Embedding Centers Hi Everyone: I am also in the market for a new paraffin embedding center. I have demo-ed or site-visited the Sakura TEK5, the Leica EG1150, and the Thermo-Fisher HistoStar. I was wondering if people could give me their critical opinion on these, or other machines. What sorts of problems have you had with them. I currently have a 25 year old Shandon Embedding Center. I like it a lot. But I would like to find a machine with a specimen holding tank large enough to allow me to immerse 300 cassettes all at once. This is because I infiltrate brain tissue with Tissue Path Paraplast but embed with Surgipath Embedding Media So I let the cassettes sit immersed in the Surgipath for an hour or two before embedding. (Until I can buy a new processor, The Shandon's holding tank also serves as a third processing station, since my Shandon Hypercenter has only 2 wax reservoirs) I also do not feel comfortable having the cassettes sitting dry in the holding tank Thanks, Tim Wheelock Neuropathology Laboratory Harvard Brain Bank McLean Hospital Belmont, MA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] automated microtomes
Leica is making the sweetest 'tomes out there at the moment, IMHO. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Dec 5, 2012 at 7:10 AM, Lynette Pavelich lpave...@hurleymc.comwrote: I am purchasing my second Leica RM2255 automated microtome. Rene' is correct in saying that the carpal tunnel syndrome will eventually affect every advanced tech by using any microtome manually. On the RM2255, the flywheel additionally is automated. You can choose to use the flywheel manually or automated by the simultaneous pushing of two buttons. Nice feature for us oldies to ease us into automation or if you have a tiny specimen that you need to take extra care with and want to use manually. Companies are very happy to send in a demo to try for a couple weeks. Happy shopping!! ;) Lynette From: histonet-boun...@lists.utsouthwestern.edu [ histonet-boun...@lists.utsouthwestern.edu] on behalf of Rene J Buesa [ rjbu...@yahoo.com] Sent: Tuesday, December 04, 2012 1:19 PM To: Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] automated microtomes The advantage of the so called automated microtomes (the only thing automated about them is the block advance) is that they alleviate wrist effort and in some ways prevent carpal tunnel syndrome that affects some histotechs (mostly of the senior persuasion). I would go with the Leica. René J. From: Rathborne, Toni trathbo...@somerset-healthcare.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Tuesday, December 4, 2012 11:48 AM Subject: [Histonet] automated microtomes I'm looking for some opinions about the automated microtomes currently available. Which ones do most techs prefer? Which are more reliable? Is there an advantage to having a semi-automated microtome? Thanks in advance for your replies. Toni ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] automated microtomes
Thanks all for your valued opinions. I have contacted Leica, and am now waiting for a demo! From: Jay Lundgren [mailto:jaylundg...@gmail.com] Sent: Thursday, December 06, 2012 4:22 PM To: Lynette Pavelich Cc: Rene J Buesa; Rathborne, Toni; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] automated microtomes Leica is making the sweetest 'tomes out there at the moment, IMHO. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Wed, Dec 5, 2012 at 7:10 AM, Lynette Pavelich lpave...@hurleymc.commailto:lpave...@hurleymc.com wrote: I am purchasing my second Leica RM2255 automated microtome. Rene' is correct in saying that the carpal tunnel syndrome will eventually affect every advanced tech by using any microtome manually. On the RM2255, the flywheel additionally is automated. You can choose to use the flywheel manually or automated by the simultaneous pushing of two buttons. Nice feature for us oldies to ease us into automation or if you have a tiny specimen that you need to take extra care with and want to use manually. Companies are very happy to send in a demo to try for a couple weeks. Happy shopping!! ;) Lynette From: histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edumailto:histonet-boun...@lists.utsouthwestern.edu] on behalf of Rene J Buesa [rjbu...@yahoo.commailto:rjbu...@yahoo.com] Sent: Tuesday, December 04, 2012 1:19 PM To: Rathborne, Toni; histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] automated microtomes The advantage of the so called automated microtomes (the only thing automated about them is the block advance) is that they alleviate wrist effort and in some ways prevent carpal tunnel syndrome that affects some histotechs (mostly of the senior persuasion). I would go with the Leica. René J. From: Rathborne, Toni trathbo...@somerset-healthcare.commailto:trathbo...@somerset-healthcare.com To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Sent: Tuesday, December 4, 2012 11:48 AM Subject: [Histonet] automated microtomes I'm looking for some opinions about the automated microtomes currently available. Which ones do most techs prefer? Which are more reliable? Is there an advantage to having a semi-automated microtome? Thanks in advance for your replies. Toni ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] refurbished histology equipment
Does anyone have a favorite vendor they would recommend to me? I am mostly looking for cryostats right now, but interested in other things, tissue processors, IHC stainers, etc. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting, LLC 40864 Arkansas Ave Bennett, CO 80102 Phone: 303-644-4538 Fax: 720-859-4110 mailto:pru...@ihctech.net pru...@ihctech.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Alcian Blue
Hi Everyone: Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue and some say 10 min?Our control works well at 10 minutes but today I had a Dr. say that he expected a small amount of cells to stain and they didn't in an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've read say 30 min, that's what would be best? Thanks Sheila ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
