[Histonet] RE: Processor dehydration cycles..

2013-02-08 Thread Susan.Walzer
70,80 95,95,100,100

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of PRESZLER, 
JEREMIAH C MSgt USAF AETC 59 LSQ/SGVLH
Sent: Thursday, February 07, 2013 11:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: Processor dehydration cycles..


I agree with Joyce on this: Formalin salt precipitate tends to become more 
common if you start above 70%. WE use a 70%, then 80% and two 95% in our 
process here.


Very Respectfully,

Jeremiah C. Preszler, MSgt, USAF HT (ASCP) Flight Chief, Anatomic Pathology
959 CSPS/ SGVLH
WHASC
JBSA-Lackland AFB, TX 78236
(210) 292-5519
DSN: 662-5519




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[Histonet] Cassette labeling

2013-02-08 Thread SimmsColon, Janine
Good morning all, 

 

I hope all here in the East are prepared for the storm, (considering I
am in New England and it is February it is not all that surprising to me
yet I am always amazed at the commotion that occurs with every impending
snow, but I digress). I know many out there may have cassette labelers
so this may not be an issue but for those who still label by hand I am
curious as to how you label. At my previous lab besides the case number
(S13-accession number), we also indicated how to embed the pieces (= for
on edge), and the number of pieces in the cassette. I am just curious as
to how common this practice is or if there is any other way you annotate
the number of pieces expected in a cassette. Thank you in advance for
any feedback you provide as I have received many helpful responses to my
previous posts.

 

Janine Simms Colon, BHA, CPhT, HT(ASCP)

Histology/Pathology

Johnson Memorial Hospital

201 Chestnut Hill Road

Stafford Springs, CT 06076

Office: 860-684-8230 ext. 5197

janine.simmsco...@jmmc.com

 


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[Histonet] Temperatures

2013-02-08 Thread Sullivan, Beatrice
I'm looking for a fix to our problem of no temperatures being taken on the 
weekends. We are closed and this is creating an issue. Our processors are not 
running until Sunday night but the paraffin in both the processors and 
embedding center are kept molten. Any help would be greatly appreciated

Beatrice L. Sullivan HT(ASCP)HTL
Corporate Histology Manager
Virtua, Voorhees
856-247-3144


This message, and any included attachments, are from Virtua Health or its 
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[Histonet] RE: Cassette labeling

2013-02-08 Thread Margiotta-Watz, Michele
We print out an embedding log and note the # of pieces/block from the Doc's 
dictation. We are small scale so it works fine for us.

Michele

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of SimmsColon, 
Janine
Sent: Friday, February 08, 2013 7:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette labeling

Good morning all, 

 

I hope all here in the East are prepared for the storm, (considering I am in 
New England and it is February it is not all that surprising to me yet I am 
always amazed at the commotion that occurs with every impending snow, but I 
digress). I know many out there may have cassette labelers so this may not be 
an issue but for those who still label by hand I am curious as to how you 
label. At my previous lab besides the case number (S13-accession number), we 
also indicated how to embed the pieces (= for on edge), and the number of 
pieces in the cassette. I am just curious as to how common this practice is or 
if there is any other way you annotate the number of pieces expected in a 
cassette. Thank you in advance for any feedback you provide as I have received 
many helpful responses to my previous posts.

 

Janine Simms Colon, BHA, CPhT, HT(ASCP)

Histology/Pathology

Johnson Memorial Hospital

201 Chestnut Hill Road

Stafford Springs, CT 06076

Office: 860-684-8230 ext. 5197

janine.simmsco...@jmmc.com

 


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Re: [Histonet] Cassette labeling

2013-02-08 Thread Cristi Rigazio
We print out a procedure list that has the number of pieces on it and it next 
to the embedding center for reference while performing that task.  The only we 
write on the cassette is the accession number.
Thanks,
Cristi

Sent from my iPhone

On Feb 8, 2013, at 4:45 AM, SimmsColon, Janine janine.simmsco...@jmmc.com 
wrote:

 Good morning all, 
 
 
 
 I hope all here in the East are prepared for the storm, (considering I
 am in New England and it is February it is not all that surprising to me
 yet I am always amazed at the commotion that occurs with every impending
 snow, but I digress). I know many out there may have cassette labelers
 so this may not be an issue but for those who still label by hand I am
 curious as to how you label. At my previous lab besides the case number
 (S13-accession number), we also indicated how to embed the pieces (= for
 on edge), and the number of pieces in the cassette. I am just curious as
 to how common this practice is or if there is any other way you annotate
 the number of pieces expected in a cassette. Thank you in advance for
 any feedback you provide as I have received many helpful responses to my
 previous posts.
 
 
 
 Janine Simms Colon, BHA, CPhT, HT(ASCP)
 
 Histology/Pathology
 
 Johnson Memorial Hospital
 
 201 Chestnut Hill Road
 
 Stafford Springs, CT 06076
 
 Office: 860-684-8230 ext. 5197
 
 janine.simmsco...@jmmc.com
 
 
 
 
 The information contained in this message may be privileged and confidential 
 and protected from disclosure. 
 If the reader of this message is not the intended recipient, or an employee 
 or agent responsible for delivering 
 this message to the intended recipient, you are hereby notified that any 
 dissemination, distribution or 
 copying of this communication is strictly prohibited. If you have received 
 this communication in error, please 
 notify us immediately by replying to the message and deleting it from your 
 computer.
 
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[Histonet] hand pap stains for non-gyn specimens/smears

2013-02-08 Thread Nancy Schmitt
Good Morning-

Would anyone be willing to share their process for pap stains - to include 
times in all steps and brand of stain you are using.  We are trying to clean up 
some issues we are having.

Thanks so much-
Nancy Schmitt HT, MLT (ASCP)
Histology Coordinator
Dubuque, IA



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[Histonet] RE: Temperatures

2013-02-08 Thread Susan.Walzer
We have the clinical lab check our friges and freezers on the 
weekends..everything else is off.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sullivan, 
Beatrice
Sent: Friday, February 08, 2013 7:52 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Temperatures

I'm looking for a fix to our problem of no temperatures being taken on the 
weekends. We are closed and this is creating an issue. Our processors are not 
running until Sunday night but the paraffin in both the processors and 
embedding center are kept molten. Any help would be greatly appreciated

Beatrice L. Sullivan HT(ASCP)HTL
Corporate Histology Manager
Virtua, Voorhees
856-247-3144


This message, and any included attachments, are from Virtua Health or its 
related affiliates and is intended only for the addressee(s). The information 
contained herein is privileged, proprietary or may include confidential 
information and/or protected patient health information. Any unauthorized 
review, forwarding, printing, copying, distributing, or otherwise disseminating 
or taking any action based on such information is strictly prohibited. If you 
have received this message in error, or have reason to believe you are not 
authorized to receive it, please delete this message promptly and notify the 
sender by e-mail with a copy to issecur...@virtua.org. 

Thank you

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Re: [Histonet] Temperatures

2013-02-08 Thread Rene J Buesa
And my question is: why are you bothered by not knowing the temp. in a 
processor that only starts to process on Sundays?
If the processor is emptied on Mondays to embed→cut, take the temp. then.
Other that this you would have to place a sensor and a recording device or you 
would have to make somebody to go to the lab. For just write down the melted 
paraffin temp.? Too much expense and trouble for an non-existent problem.
René J.

From: Sullivan, Beatrice bsulli...@virtua.org
To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu 
Sent: Friday, February 8, 2013 7:52 AM
Subject: [Histonet] Temperatures

I'm looking for a fix to our problem of no temperatures being taken on the 
weekends. We are closed and this is creating an issue. Our processors are not 
running until Sunday night but the paraffin in both the processors and 
embedding center are kept molten. Any help would be greatly appreciated

Beatrice L. Sullivan HT(ASCP)HTL
Corporate Histology Manager
Virtua, Voorhees
856-247-3144


This message, and any included attachments, are from Virtua Health or its 
related affiliates and is intended only for the addressee(s). The information 
contained herein is privileged, proprietary or may include confidential 
information and/or protected patient health information. Any unauthorized 
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or taking any action based on such information is strictly prohibited. If you 
have received this message in error, or have reason to believe you are not 
authorized to receive it, please delete this message promptly and notify the 
sender by e-mail with a copy to issecur...@virtua.org. 

Thank you

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RE: [Histonet] Temperatures

2013-02-08 Thread Sullivan, Beatrice
Because I have been told that according to JCAHO (who will be inspecting us) 
that if a temperature is required to ascertain consistency, quality and 
integrity, you will have to record temperature 7 days per week.

From: Rene J Buesa [mailto:rjbu...@yahoo.com]
Sent: Friday, February 08, 2013 10:17 AM
To: Sullivan, Beatrice; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Temperatures

And my question is: why are you bothered by not knowing the temp. in a 
processor that only starts to process on Sundays?
If the processor is emptied on Mondays to embed→cut, take the temp. then.
Other that this you would have to place a sensor and a recording device or you 
would have to make somebody to go to the lab. For just write down the melted 
paraffin temp.? Too much expense and trouble for an non-existent problem.
René J.

From: Sullivan, Beatrice bsulli...@virtua.orgmailto:bsulli...@virtua.org
To: 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu
Sent: Friday, February 8, 2013 7:52 AM
Subject: [Histonet] Temperatures

I'm looking for a fix to our problem of no temperatures being taken on the 
weekends. We are closed and this is creating an issue. Our processors are not 
running until Sunday night but the paraffin in both the processors and 
embedding center are kept molten. Any help would be greatly appreciated

Beatrice L. Sullivan HT(ASCP)HTL
Corporate Histology Manager
Virtua, Voorhees
856-247-3144


This message, and any included attachments, are from Virtua Health or its 
related affiliates and is intended only for the addressee(s). The information 
contained herein is privileged, proprietary or may include confidential 
information and/or protected patient health information. Any unauthorized 
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or taking any action based on such information is strictly prohibited. If you 
have received this message in error, or have reason to believe you are not 
authorized to receive it, please delete this message promptly and notify the 
sender by e-mail with a copy to 
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Thank you

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RE: [Histonet] Temperatures

2013-02-08 Thread Tom McNemar
We are JCAHO and this has never come up but that doesn't mean that it won't.

I don’t know about your specific processor but ours (VIP5), will fail to start 
and alarm if the paraffin temp is out of range.  Our processor is tied in to 
the main lab temperature monitoring system so we get a call anytime there is an 
alarm.

Such a system is probably not feasible for a processor alone but does your main 
lab have anything in place?

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sullivan, 
Beatrice
Sent: Friday, February 08, 2013 10:32 AM
To: Rene J Buesa; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Temperatures

Because I have been told that according to JCAHO (who will be inspecting us) 
that if a temperature is required to ascertain consistency, quality and 
integrity, you will have to record temperature 7 days per week.

From: Rene J Buesa [mailto:rjbu...@yahoo.com]
Sent: Friday, February 08, 2013 10:17 AM
To: Sullivan, Beatrice; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Temperatures

And my question is: why are you bothered by not knowing the temp. in a 
processor that only starts to process on Sundays?
If the processor is emptied on Mondays to embed→cut, take the temp. then.
Other that this you would have to place a sensor and a recording device or you 
would have to make somebody to go to the lab. For just write down the melted 
paraffin temp.? Too much expense and trouble for an non-existent problem.
René J.

From: Sullivan, Beatrice bsulli...@virtua.orgmailto:bsulli...@virtua.org
To: 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu
Sent: Friday, February 8, 2013 7:52 AM
Subject: [Histonet] Temperatures

I'm looking for a fix to our problem of no temperatures being taken on the 
weekends. We are closed and this is creating an issue. Our processors are not 
running until Sunday night but the paraffin in both the processors and 
embedding center are kept molten. Any help would be greatly appreciated

Beatrice L. Sullivan HT(ASCP)HTL
Corporate Histology Manager
Virtua, Voorhees
856-247-3144


This message, and any included attachments, are from Virtua Health or its 
related affiliates and is intended only for the addressee(s). The information 
contained herein is privileged, proprietary or may include confidential 
information and/or protected patient health information. Any unauthorized 
review, forwarding, printing, copying, distributing, or otherwise disseminating 
or taking any action based on such information is strictly prohibited. If you 
have received this message in error, or have reason to believe you are not 
authorized to receive it, please delete this message promptly and notify the 
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Thank you

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RE: [Histonet] Temperatures

2013-02-08 Thread Kuhnla, Melissa
Beatrice,
How about...your processor monitors the temp.  If it falls out of a certain 
range it will most likely throw an error.  This is not a recorded temp day 6 
and 7 but is in a sense a monitoring system.  Our processors are set up with an 
alarm system that call employees if certain errors occur.  Do you have 
something similar?

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sullivan, 
Beatrice
Sent: Friday, February 08, 2013 10:32 AM
To: Rene J Buesa; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Temperatures

Because I have been told that according to JCAHO (who will be inspecting us) 
that if a temperature is required to ascertain consistency, quality and 
integrity, you will have to record temperature 7 days per week.

From: Rene J Buesa [mailto:rjbu...@yahoo.com]
Sent: Friday, February 08, 2013 10:17 AM
To: Sullivan, Beatrice; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Temperatures

And my question is: why are you bothered by not knowing the temp. in a 
processor that only starts to process on Sundays?
If the processor is emptied on Mondays to embed→cut, take the temp. then.
Other that this you would have to place a sensor and a recording device or you 
would have to make somebody to go to the lab. For just write down the melted 
paraffin temp.? Too much expense and trouble for an non-existent problem.
René J.

From: Sullivan, Beatrice bsulli...@virtua.orgmailto:bsulli...@virtua.org
To: 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu
Sent: Friday, February 8, 2013 7:52 AM
Subject: [Histonet] Temperatures

I'm looking for a fix to our problem of no temperatures being taken on the 
weekends. We are closed and this is creating an issue. Our processors are not 
running until Sunday night but the paraffin in both the processors and 
embedding center are kept molten. Any help would be greatly appreciated

Beatrice L. Sullivan HT(ASCP)HTL
Corporate Histology Manager
Virtua, Voorhees
856-247-3144


This message, and any included attachments, are from Virtua Health or its 
related affiliates and is intended only for the addressee(s). The information 
contained herein is privileged, proprietary or may include confidential 
information and/or protected patient health information. Any unauthorized 
review, forwarding, printing, copying, distributing, or otherwise disseminating 
or taking any action based on such information is strictly prohibited. If you 
have received this message in error, or have reason to believe you are not 
authorized to receive it, please delete this message promptly and notify the 
sender by e-mail with a copy to 
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[Histonet] RE: Cassette labeling

2013-02-08 Thread Podawiltz, Thomas
That is basically what we do. 

Tom

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Margiotta-Watz, 
Michele
Sent: Friday, February 08, 2013 7:55 AM
To: 'SimmsColon, Janine'; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cassette labeling

We print out an embedding log and note the # of pieces/block from the Doc's 
dictation. We are small scale so it works fine for us.

Michele

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of SimmsColon, 
Janine
Sent: Friday, February 08, 2013 7:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cassette labeling

Good morning all, 

 

I hope all here in the East are prepared for the storm, (considering I am in 
New England and it is February it is not all that surprising to me yet I am 
always amazed at the commotion that occurs with every impending snow, but I 
digress). I know many out there may have cassette labelers so this may not be 
an issue but for those who still label by hand I am curious as to how you 
label. At my previous lab besides the case number (S13-accession number), we 
also indicated how to embed the pieces (= for on edge), and the number of 
pieces in the cassette. I am just curious as to how common this practice is or 
if there is any other way you annotate the number of pieces expected in a 
cassette. Thank you in advance for any feedback you provide as I have received 
many helpful responses to my previous posts.

 

Janine Simms Colon, BHA, CPhT, HT(ASCP)

Histology/Pathology

Johnson Memorial Hospital

201 Chestnut Hill Road

Stafford Springs, CT 06076

Office: 860-684-8230 ext. 5197

janine.simmsco...@jmmc.com

 


The information contained in this message may be privileged and confidential 
and protected from disclosure. 
If the reader of this message is not the intended recipient, or an employee or 
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are hereby notified that any dissemination, distribution or copying of this 
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Re: [Histonet] RE: Temperatures

2013-02-08 Thread Paula Sicurello
We have NIST traceable digital thermometers that can have the min/max
reset.  We have been told that if you reset on Friday and check on
Monday morning the min/max  it is in range-put an OK by the date.

-- 
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P:  919.684.2091


On Fri, Feb 8, 2013 at 9:30 AM,  susan.wal...@hcahealthcare.com wrote:
 We have the clinical lab check our friges and freezers on the 
 weekends..everything else is off.

 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sullivan, 
 Beatrice
 Sent: Friday, February 08, 2013 7:52 AM
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Temperatures

 I'm looking for a fix to our problem of no temperatures being taken on the 
 weekends. We are closed and this is creating an issue. Our processors are not 
 running until Sunday night but the paraffin in both the processors and 
 embedding center are kept molten. Any help would be greatly appreciated

 Beatrice L. Sullivan HT(ASCP)HTL
 Corporate Histology Manager
 Virtua, Voorhees
 856-247-3144


 This message, and any included attachments, are from Virtua Health or its 
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[Histonet] PIN4 staining

2013-02-08 Thread Cathy Crumpton
We use Bond slides and have noticed that occasionally they seem to have an 
extra thick coating on them that will pick up DAB and turn slides brown.  If 
the glass itself is turning brown this might be your issue.  Have you tried 
different slide lots or brands?

Cathy Crumpton HT(ASCP), Lead Histotechnician

Tuality Community Hospital
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[Histonet] Could these samples have been saved?

2013-02-08 Thread Johnson, Kevin
Dear all,

During an overnight tissue processing cycle, a malfunction occurred such that 
the sample basket was suspended in mid-air for several hours at probably the 
worst spot in which to do so---after the final absolute ethanol of the 
dehydration series. I continued the process manually in the morning, and 
carried it through blocking and attempted sectioning.  However, the samples 
(mouse skin and fat) had been converted to uncuttable rocks.

In hindsight, should I have attempted to rehydrate and reprocess these samples 
in an attempt to glean even minimal information from them?  Or is there no way 
to unmummify a mummy?

Regards,

Kevin Johnson
University of Miami
Diabetes Research Institute
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[Histonet] What's on that HE?

2013-02-08 Thread Bruce Gapinski
Dear Histonians,
How many sections do put on a slide from a block cast in a 
small embedding mold? Do you automatically include any levels?  Then please 
tell me how many slides your techs cut per hour?
The problem is (may be) that we put 4 levels on a slide with 
two sections per level. That is:

  1.  Full face (or pretty close) 2 sections
  2.  Level 2 sections
  3.  Level 2 sections
  4.  Level 2 sections
So I have 8 sections on my HE. This takes me some time, and I understand all 
histologists may not be doing things this way. But the problem is, when we 
discuss output and my techs appear slow I find that other labs put much less 
tissue on a slide. Some labs give a fatty string of serial sections in two 
rows, and that appears to be just like my slide. But only on the surface 
(pardon the pun).
My pathologists just renegotiated the contracts for all our dermatologists 
after the new 88305TC pricing, and those doctors say Oh, we can get slides 
cheaper than that. Maybe they can but what would they see on the HE, and 
would they care?
So now I'm faced with doing things as we have or stop giving those 
levels. I understand my job as a Histologist is to demonstrate the tissue. So 
I'll stick to the levels, because  I:

1.   Work for the patient

2.   Am supervised by the Pathologists

3.   Am reimbursed by our company
Tell me what you do in you lab, please

Bruce Gapinsk HT (ASCP)
Chief Histologist
Marin Medical Laboratories
PathGroup SF




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Re: [Histonet] Could these samples have been saved?

2013-02-08 Thread Roger Heyna
We microwave process our tissues, and we've had tissues fall out of the rack 
after the dehydration phases and air dry in the open retort for hours. We did 
not continue with processing; we added them to formalin for eight hours (which 
could be overkill) and then started the processing from the beginning. We had 
no problems with cutting or staining.
 
If you look in Freida Carson's Histotechnology: A Self-Instructional Text, she 
includes a protocol for tissues that were well-fixed but accidentally 
desiccated. She suggests the following:
 
1. If the tissue has been in paraffin, blot off as much as possible with paper 
towels.
2. Soak the tissues overnight in a rehydrating solution: 50 mL water, 30 mL 
absolute alcohol, and 20 mL of 5% aqueous solution of sodium carbonate.
3. Reprocess as usual.
 
I've never done this before, and maybe others have modified it for labs without 
sodium carbonate.
 
Roger Heyna
Maywood, IL

 Johnson, Kevin kjohn...@med.miami.edu 2/8/2013 12:00 PM 
Dear all,

During an overnight tissue processing cycle, a malfunction occurred such that 
the sample basket was suspended in mid-air for several hours at probably the 
worst spot in which to do so---after the final absolute ethanol of the 
dehydration series. I continued the process manually in the morning, and 
carried it through blocking and attempted sectioning.  However, the samples 
(mouse skin and fat) had been converted to uncuttable rocks.

In hindsight, should I have attempted to rehydrate and reprocess these samples 
in an attempt to glean even minimal information from them?  Or is there no way 
to unmummify a mummy?

Regards,

Kevin Johnson
University of Miami
Diabetes Research Institute
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Re: [Histonet] Could these samples have been saved?

2013-02-08 Thread kgrobert
I don't know about your samples now, but for future reference, you might
find this interesting:
http://realhistoryww.com/world_history/ancient/Misc/Data/Study_mummified_soft_tissues.htm

Kathleen Roberts

Principal Lab Technician
Neurotoxicology Labs
Molecular Pathology Facility Core
Dept of Pharmacology  Toxicology
Rutgers, the State University of NJ
41 B Gordon Road
Piscataway, NJ 08854
(848) 445-1443
FAX (732) 445-6905

 Dear all,

 During an overnight tissue processing cycle, a malfunction occurred such
 that the sample basket was suspended in mid-air for several hours at
 probably the worst spot in which to do so---after the final absolute
 ethanol of the dehydration series. I continued the process manually in the
 morning, and carried it through blocking and attempted sectioning.
 However, the samples (mouse skin and fat) had been converted to uncuttable
 rocks.

 In hindsight, should I have attempted to rehydrate and reprocess these
 samples in an attempt to glean even minimal information from them?  Or is
 there no way to unmummify a mummy?

 Regards,

 Kevin Johnson
 University of Miami
 Diabetes Research Institute
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[Histonet] RE: Histonet Digest, Vol 111, Issue 13

2013-02-08 Thread Santiago, Albert
Hi Janine, we use cassette printers but we still write the number of pieces on 
one side of the cassette and any special  instructions on the other side, such 
as embed on edge or embed on red ink, etc.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
histonet-requ...@lists.utsouthwestern.edu
Sent: Friday, February 08, 2013 1:01 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 111, Issue 13

Send Histonet mailing list submissions to
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When replying, please edit your Subject line so it is more specific
than Re: Contents of Histonet digest...


Today's Topics:

   1. RE: Temperatures (Sullivan, Beatrice)
   2. RE: Temperatures (Tom McNemar)
   3. RE: Temperatures (Kuhnla, Melissa)
   4. RE: Cassette labeling (Podawiltz, Thomas)
   5. Re: RE: Temperatures (Paula Sicurello)
   6. PIN4 staining (Cathy Crumpton)


--

Message: 1
Date: Fri, 8 Feb 2013 15:32:22 +
From: Sullivan, Beatrice bsulli...@virtua.org
Subject: RE: [Histonet] Temperatures
To: Rene J Buesa rjbu...@yahoo.com,
histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:
6932520047f7ee46b512e9801344f160046...@exchangemb-1.virtua.org
Content-Type: text/plain; charset=utf-8

Because I have been told that according to JCAHO (who will be inspecting us) 
that if a temperature is required to ascertain consistency, quality and 
integrity, you will have to record temperature 7 days per week.

From: Rene J Buesa [mailto:rjbu...@yahoo.com]
Sent: Friday, February 08, 2013 10:17 AM
To: Sullivan, Beatrice; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Temperatures

And my question is: why are you bothered by not knowing the temp. in a 
processor that only starts to process on Sundays?
If the processor is emptied on Mondays to embed→cut, take the temp. then.
Other that this you would have to place a sensor and a recording device or you 
would have to make somebody to go to the lab. For just write down the melted 
paraffin temp.? Too much expense and trouble for an non-existent problem.
René J.

From: Sullivan, Beatrice bsulli...@virtua.orgmailto:bsulli...@virtua.org
To: 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu
Sent: Friday, February 8, 2013 7:52 AM
Subject: [Histonet] Temperatures

I'm looking for a fix to our problem of no temperatures being taken on the 
weekends. We are closed and this is creating an issue. Our processors are not 
running until Sunday night but the paraffin in both the processors and 
embedding center are kept molten. Any help would be greatly appreciated

Beatrice L. Sullivan HT(ASCP)HTL
Corporate Histology Manager
Virtua, Voorhees
856-247-3144


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or taking any action based on such information is strictly prohibited. If you 
have received this message in error, or have reason to believe you are not 
authorized to receive it, please delete this message promptly and notify the 
sender by e-mail with a copy to 
issecur...@virtua.orgmailto:issecur...@virtua.org.

Thank you

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This message, and any included attachments, are from Virtua Health or its 
related affiliates and is intended only for the addressee(s). The information 
contained herein is privileged, proprietary or may include confidential 
information and/or protected patient health information. Any unauthorized 
review, forwarding, printing, copying, distributing, or otherwise disseminating 
or taking any action based on such information is strictly prohibited. If you 
have received this message in error, or have reason to believe you are not 
authorized to receive it, please delete this message promptly and notify the 
sender by e-mail with a copy to issecur...@virtua.org. 

Thank you


--

Message: 2
Date: Fri, 8 Feb 2013 11:22:59 -0500
From: Tom McNemar 

[Histonet] Georgia Society for Histotechnology 40th year Celebration

2013-02-08 Thread Zimmerman, Billie
Please join us on beautiful Jekyll Island this April 12-14, 2013.  If you 
register by March 1, 2013, you will receive the discounted all inclusive rate 
of $115. After March 1st, the all inclusive rate is $135.  This rate includes 
the vendor reception on Friday night, celebration luncheon on Saturday, as well 
as a continental breakfast both Saturday and Sunday. In addition, you will 
receive all your CEU's through NSH with a possible total of 15 CEU's.  
Registration and room reservations are on our website at the symposium link.  
www.histosearch.com/gsh/http://www.histosearch.com/gsh/
I encourage you to join or renew your FREE membership with the Georgia Society 
for Histotechnology.  We have an impressive offering of accomplished speakers 
from our discipline.  Please don't miss this affordable opportunity for 
education, networking, and celebrating Histotechnology.

Hope to see you on the island!

Wanda K. Simons HT(ASCP)
GSH President
Augusta State University and Georgia Health Sciences University have 
consolidated to become Georgia Regents University. Effective January 9, 2013, 
my email address has changed to bzimm...@gru.edu. Please update your address 
book to reflect this change.
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[Histonet] CYP 04300

2013-02-08 Thread PicheGrocki, Jessica
Hi All,

I have a question from our Cytology department. For question CYP 04300, how is 
everyone managing their QC slides for cytology stains? Are you doing separate 
QC slides for thin preps, smears, FNA's etc?

Thanks,

Jessica Piche' HT(ASCP)
Waterbury Hospital



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notify the sender immediately and delete these documents. Copyright (c) 
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RE: [Histonet] What's on that HE?

2013-02-08 Thread joelle weaver

Just depends on the protocol at that laboratory- often at the discretion of the 
pathologist(s) reading or the medical director- I guess all variations  are 
acceptable so long as all the sections are of good quality, they may not 
want/need serials, but prefer levels, and if  they meet the diagnostic needs, 
and are representative of the tissue sample.  I think you could do things a lot 
of different ways dependant on the tissue type and clinical differential. I 
have seen and done many of the variations you mention in your post, up to 9-12 
serial sections ( rows of  3 ribbons on one slide) and up to 12 levels ( 
specific depth into the block) on single or multiple slides., with special 
stains and IHC levels thrown in as well- so sky's the limit  to me as to what 
might work for your lab and pathologists. Sometimes you don't really need to 
provide all the extra sections, but for some specimens that might make sense. 
So I like it when the protocols are pretty specific- though that variability 
may not apply to your lab if you do only a few tissue types. Anyhow I would 
rather do all that is likely to be needed the first time into the block, no 
matter how complicated the protocol,  than have to recut it again and again. 
You might end up losing too much tissue that you need later for molecular or 
other testing too. Outside numbers are a good initial benchmark to me, but your 
own productivity statistics and staff are often a lot more meaningful. I have 
just seen different numbers for rates on these protocols versus single section 
slides.  Maybe do a side by side, of having them read single section slides  
and then the same specimen type ( as similar as possible) with other different 
sectioning using complex  protocols with times to produce in sectioning time, 
stain throughput, all variables you can think of- maybe they may have different 
opinions then? Just an idea.



Joelle Weaver MAOM, HTL (ASCP) QIHC
  From: bgapin...@pathgroup.com
 To: histonet@lists.utsouthwestern.edu
 Date: Fri, 8 Feb 2013 18:00:11 +
 Subject: [Histonet] What's on that HE?
 
 Dear Histonians,
 How many sections do put on a slide from a block cast in a 
 small embedding mold? Do you automatically include any levels?  Then please 
 tell me how many slides your techs cut per hour?
 The problem is (may be) that we put 4 levels on a slide with 
 two sections per level. That is:
 
   1.  Full face (or pretty close) 2 sections
   2.  Level 2 sections
   3.  Level 2 sections
   4.  Level 2 sections
 So I have 8 sections on my HE. This takes me some time, and I understand all 
 histologists may not be doing things this way. But the problem is, when we 
 discuss output and my techs appear slow I find that other labs put much less 
 tissue on a slide. Some labs give a fatty string of serial sections in two 
 rows, and that appears to be just like my slide. But only on the surface 
 (pardon the pun).
 My pathologists just renegotiated the contracts for all our dermatologists 
 after the new 88305TC pricing, and those doctors say Oh, we can get slides 
 cheaper than that. Maybe they can but what would they see on the HE, and 
 would they care?
 So now I'm faced with doing things as we have or stop giving those 
 levels. I understand my job as a Histologist is to demonstrate the tissue. So 
 I'll stick to the levels, because  I:
 
 1.   Work for the patient
 
 2.   Am supervised by the Pathologists
 
 3.   Am reimbursed by our company
 Tell me what you do in you lab, please
 
 Bruce Gapinsk HT (ASCP)
 Chief Histologist
 Marin Medical Laboratories
 PathGroup SF
 
 
 
 
 Important Notice: This e-mail is intended for the use of the person to whom 
 it is addressed and may contain information that is privileged and 
 confidential. If you are not the intended recipient, any disclosure, copying, 
 distribution, or use of the contents of this message is strictly prohibited. 
 If you have received this e-mail in error, please destroy this message and 
 contact the Security Officer at PathGroup, Inc immediately at 615-562-9255. 
 Thank you
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RE: [Histonet] Could these samples have been saved?

2013-02-08 Thread joelle weaver

It was my understanding that with MW-assited processing, that they could not be 
put back through the MW assisted fixation part of the program. That is not to 
say that you couldn't rehydrate tissues and then evaluate and pass them through 
the rest if they were fixed, such as the alcohols, isopropanol and/or paraffin. 
I supposed you could conventionally fix if that were the issue, but I do 
remember when using these instruments that I didn't  double microwave. 
Instead,  I just made an edited program to take it to wax depending on the 
situation. Most of the software made this pretty easy to do. Does anyone know 
differently? 




Joelle Weaver MAOM, HTL (ASCP) QIHC
  Date: Fri, 8 Feb 2013 13:22:15 -0500
 From: kgrob...@rci.rutgers.edu
 To: kjohn...@med.miami.edu
 Subject: Re: [Histonet] Could these samples have been saved?
 CC: histonet@lists.utsouthwestern.edu
 
 I don't know about your samples now, but for future reference, you might
 find this interesting:
 http://realhistoryww.com/world_history/ancient/Misc/Data/Study_mummified_soft_tissues.htm
 
 Kathleen Roberts
 
 Principal Lab Technician
 Neurotoxicology Labs
 Molecular Pathology Facility Core
 Dept of Pharmacology  Toxicology
 Rutgers, the State University of NJ
 41 B Gordon Road
 Piscataway, NJ 08854
 (848) 445-1443
 FAX (732) 445-6905
 
  Dear all,
 
  During an overnight tissue processing cycle, a malfunction occurred such
  that the sample basket was suspended in mid-air for several hours at
  probably the worst spot in which to do so---after the final absolute
  ethanol of the dehydration series. I continued the process manually in the
  morning, and carried it through blocking and attempted sectioning.
  However, the samples (mouse skin and fat) had been converted to uncuttable
  rocks.
 
  In hindsight, should I have attempted to rehydrate and reprocess these
  samples in an attempt to glean even minimal information from them?  Or is
  there no way to unmummify a mummy?
 
  Regards,
 
  Kevin Johnson
  University of Miami
  Diabetes Research Institute
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[Histonet] Temps

2013-02-08 Thread Chladek, Virginia
RE: temperatures

We press reset on the thermometers on Friday, then record the min and max temps 
on the Monday after. As long as those are within range then we are ok...

Virginia Chladek, HTL FL


Message: 1
Date: Fri, 8 Feb 2013 15:32:22 +
From: Sullivan, Beatrice bsulli...@virtua.org
Subject: RE: [Histonet] Temperatures
To: Rene J Buesa rjbu...@yahoo.com,
histonet@lists.utsouthwestern.edu
histonet@lists.utsouthwestern.edu
Message-ID:
6932520047f7ee46b512e9801344f160046...@exchangemb-1.virtua.org
Content-Type: text/plain; charset=utf-8

Because I have been told that according to JCAHO (who will be inspecting us) 
that if a temperature is required to ascertain consistency, quality and 
integrity, you will have to record temperature 7 days per week.
,

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Re: [Histonet] Could these samples have been saved?

2013-02-08 Thread Rene J Buesa
You are right. You should have tried to rehydrate the tissues, especially mouse 
that is very lean in itself.
You cannot unmummify them now.
René J.

From: Johnson, Kevin kjohn...@med.miami.edu
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu 
Sent: Friday, February 8, 2013 1:00 PM
Subject: [Histonet] Could these samples have been saved?

Dear all,

During an overnight tissue processing cycle, a malfunction occurred such that 
the sample basket was suspended in mid-air for several hours at probably the 
worst spot in which to do so---after the final absolute ethanol of the 
dehydration series. I continued the process manually in the morning, and 
carried it through blocking and attempted sectioning.  However, the samples 
(mouse skin and fat) had been converted to uncuttable rocks.

In hindsight, should I have attempted to rehydrate and reprocess these samples 
in an attempt to glean even minimal information from them?  Or is there no way 
to unmummify a mummy?

Regards,

Kevin Johnson
University of Miami
Diabetes Research Institute
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[Histonet] Histology QA Manager opening at Kaiser Berkeley, CA

2013-02-08 Thread Kiranjit Grewal
Hi Histonetters,
 
Great opportunity to work for one of the best Histology Lab. Excellent benefits 
and tremendous growth opportunities in the organization.
 
Here is the posting and please contact me if you have any questions or apply on 
line.
 
Regional Lab QA Section Manager, Histology [BRK067]( Job # 154196)
 


Description  
Under the general direction of the Laboratory Medical Director and the 
immediate supervision of the Pathology Director and Director of Laboratory 
Services, Quality and collaboration with Histology operational managers, NCAL 
pathology departments and Regional Laboratory Histology Director, directs and 
controls the Histology departmental specific Laboratory Quality 
System/Assessment program to improve quality, services, and meet all regulatory 
requirements.
 
Essential Functions:
• Manages all histology activities, including recruiting, hiring and training 
of staff.
• Ensures competency, motivates and encourages professional growth.
• Controls costs by monitoring productivity, personnel utilization, overtime, 
material usage rates, analyzing fluctuations in types and volumes of tests, and 
implements corrective actions.
• Participates in the design of Regional Laboratory and Northern California 
Region integrated laboratory quality system. 
• Ensures compliance with regulatory and accreditation agencies' rules and 
regulations.
•  Designs and implements effective risk control processes.
• Leads in the research of new and/or improved test development methodologies 
by:  performing experimental testing procedures;  validating 
effectiveness/feasibility for implementation;  cost of procedure(s);  preparing 
and submitting recommendation(s) for change to laboratory management and other 
stakeholders (such as Chiefs of Pathologists).
• Researches and resolves client problem/issues.
• Oversees and coordinates startup and implementation activities resulting from 
new services or transfer of services.
• Participates in department, inter-department, inter-facility, and 
inter-regional level projects which help the regional laboratory achieve its 
goal of providing quality service and client support in a cost effective manner.
•  Develops transition plan for new services/tests, outreach programs for 
transfer of work, timelines, and monitors milestones to achieve service 
expectations.
•  Coordinates internal resources to support new service.
• Serves as primary liaison to RILIS/ITS for ongoing and new issues. 
• Develops needs analysis as appropriate.  Leads in the integration of 
secondary laboratory information system with RILIS.
• Kaiser Permanente conducts compensation reviews of positions on a routine 
basis.
•  At any time, Kaiser Permanente reserves the right to reevaluate and change 
job descriptions, or to change such positions from salaried to hourly pay 
status. 
• Such changes are generally implemented only after notice is given to affected 
employees.
 



Qualifications  
Basic Qualifications:
• Significant experience in high-volume histology laboratory required (usually 
five years).
• Previous supervisory/managerial experience (usually three years). 
• Bachelor's in biological sciences or related field required.
• Master's in science or related field preferred.
• Additional courses in business administration and/or management preferred.
• Certification by the American Society for Clinical Pathologists.
• Must be able to work in a Labor/Management Partnership environment.
 
Preferred Qualifications:

• Minimum 5 years experience in a high volume clinical laboratory or complex 
healthcare delivery system.
• At least 3 years in recent progressive supervisory/management 
responsibilities.
• Proven strong leadership and human resource skills.
• Demonstrable skills and experience in creating a collaborative work 
environment and labor management partnership.
• Demonstrable experience in project management, quality system management, 
process mapping, and regulatory compliance.
• Demonstrable experience in high level of initiative, good judgment, exemplary 
interpersonal skills, excellent written/verbal communication skills, excellent 
presentation, and supporting all levels of internal and external clients in a 
multifaceted, culturally diverse, and complex operation.
• Demonstrable knowledge and/or skills in budgeting, purchasing, staffing, 
scheduling, and employee coaching/mentoring.
• Knowledge in bargaining union contract applications. Proficient skills in 
using various computer applications in document creation, statistical analysis, 
graphic display, and flowcharting.• Ability to be flexible in schedules to meet 
deadlines or operational requirements as needed. 
Primary Location
: California-Berkeley-Berkeley Regional Lab - 1725 1725 Eastshore  
Scheduled Hours (1-40)
: 40  
Shift
: Day  
Working Days
: Mon - Fri  
Working Hours Start
: 9:00AM  
Working Hours End
: 5:30PM 
 
 
Kiranjit Grewal
 
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[Histonet] Temperatures

2013-02-08 Thread Amos Brooks
Good Grief!
 Why would this really be an issue. The temperatures are taken
throughout the week and are constant (or you have a different problem
entirely) why would they only spike or tank on the weekend, and why would
it even matter if the equipment isn't being used. If a tree falls in the
forest and no one is around to hear it who the heck cares?

Face-palm,
Amos

On Fri, Feb 8, 2013 at 10:17 AM,
histonet-requ...@lists.utsouthwestern.eduwrote:

 Message: 16
 Date: Fri, 8 Feb 2013 12:52:29 +
 From: Sullivan, Beatrice bsulli...@virtua.org
 Subject: [Histonet] Temperatures
 To: histonet@lists.utsouthwestern.edu
 histonet@lists.utsouthwestern.edu
 Message-ID:
 6932520047f7ee46b512e9801344f160046...@exchangemb-1.virtua.org
 Content-Type: text/plain; charset=us-ascii

 I'm looking for a fix to our problem of no temperatures being taken on the
 weekends. We are closed and this is creating an issue. Our processors are
 not running until Sunday night but the paraffin in both the processors and
 embedding center are kept molten. Any help would be greatly appreciated

 Beatrice L. Sullivan HT(ASCP)HTL
 Corporate Histology Manager
 Virtua, Voorhees
 856-247-3144

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RE: [Histonet] Temperatures

2013-02-08 Thread WILLIAM DESALVO
Who cares? The patient. I am quite sure the patient wants quality assured 
results. Would any of us use a reagent or chemical from a manufacturer that 
could not prove the quality? If the instrument remains on during periods on 
inactivity in the lab, reagents or chemicals are stored in the the lab during 
the same periods, then you must monitor and prove the quality. It's kind of 
like, when we aren't around and the tree falls, we still need to know when is 
fell and if it fell on anything. Everything we do starts w/ standardization and 
because we produce patient test results, we must prove our processes 
continually meet the standard, good quality control and assurance. The 
accrediting and licensing organizations are just doing their part to check that 
we can, do and will only produce quality work, no exceptions. As has been 
previously mentioned, there are several solutions to the problem. If the lab is 
open, have someone from another area check, record and sign for the process. 
There are several electronic thermometers, w/ a probe, that can be set for a 
minimum and maximum range and accurately record temps during periods when staff 
is not available. We should never wait until we have a questionable result 
before we check for quality. 
William DeSalvo, BS HTL(ASCP)
Production Manager-Anatomic Pathology
Chair, NSH Quality Management Committee
Owner/Consultant, Collaborative Advantage Consulting

  Date: Fri, 8 Feb 2013 20:03:17 -0500
 From: amosbro...@gmail.com
 To: histonet@lists.utsouthwestern.edu
 Subject: [Histonet] Temperatures
 
 Good Grief!
  Why would this really be an issue. The temperatures are taken
 throughout the week and are constant (or you have a different problem
 entirely) why would they only spike or tank on the weekend, and why would
 it even matter if the equipment isn't being used. If a tree falls in the
 forest and no one is around to hear it who the heck cares?
 
 Face-palm,
 Amos
 
 
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Re: [Histonet] Could these samples have been saved?

2013-02-08 Thread Maxim Peshkov
Kevin!
We have had a similar problem, when our
tissues totally dried on air during
more than 18 hours after acetone and
looked as a stones.
We put our bloks into Luna's solution
for repocessing tissue with formaldehyde,
sodium acetate and glycerine
(Laboratory methods in histotechnology,
AFIP, 1992) and did as described in this chapter.
It works great. We successfully sectioned
all the blocks and have had accetable
slides for diagnosis.

Some years after we started to use
mineral oil and isopropanol for
processing. Isopropanol will not
such severe drying tissues as acetone
and ethanol.

Maxim Peshkov,
Russia,
Taganrog.

Original message---
From: Johnson, Kevin kjohn...@med.miami.edu
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu 
Sent: Friday, February 8, 2013 1:00 PM
Subject: [Histonet] Could these samples have been saved?

Dear all,

During an overnight tissue processing cycle,
a malfunction occurred such that the sample
basket was suspended in mid-air for several
hours at probably the worst spot in which to
do so---after the final absolute ethanol
of the dehydration series.
I continued the process manually in the
morning, and carried it through blocking
and attempted sectioning.
However, the samples (mouse skin and fat)
had been converted to uncuttable rocks.

In hindsight, should I have attempted to
rehydrate and reprocess these samples in
an attempt to glean even minimal information
from them?  Or is there no way to unmummify
a mummy?

Regards,

Kevin Johnson
University of Miami
Diabetes Research Institute
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--

  mailto:maxim...@mail.ru


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