[Histonet] Histology protocol for Sectioning of Drosophila melanogaster
Dear all I am searching for a histology protocol for whole fly sectioning for subsequent confocal fluorescence microscopy. Is there anyone who has ever done such an experiment and can provide a histology protocol? Thank you very much for your reply. Best wishes Carlos ETH Zurich Carlos Mora, PhD student Institute for Chemical and Bio-Engineering HCI E 112 Wolfgang-Pauli-Str. 10 CH-8093 Zurich SWITZERLAND carlos.m...@chem.ethz.chmailto:carlos.m...@chem.ethz.ch www.fml.ethz.chhttp://www.fml.ethz.ch/ mailto:e.athanass...@chem.ethz.ch+41 44 632 30 54 phone +41 44 633 15 71 fax ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Position Alert - Antibody Staining Consultant
Good morning Histonet! Our client has recently opened a new expansion histology/antibody opportunity and I wanted to reach out regarding a few specifics and see if anyone would be interested in learning more. Our client is a global leader in Histology and IHC has recently opened a new expansion opportunity in the Midwest Region to focus on driving IHC Reagent and Antibody sales. We are searching for a histology professional who has a strong background in IHC and antibodies that would be interested in working in a team sales/consultant environment. Though sales experience is a plus, this position does not require previous sales experience and this would be outstanding opportunity for someone looking to break into sales that has a strong histology background. The ideal location for the position would be in the greater Minneapolis area, however we will consider candidates in the region (MN, ND, SD, IA, NE, KS). The position offer a strong package including a base salary, competitive commission structure, car allowance, gas card, cell phone, laptop computer, and full benefits. If you or anyone you may know would be interested in the position, please contact me directly m...@personifysearch.com or my colleague Courtney Harris at c...@personifysearch.com to learn more. Regards, Matt Ward *Account Executive* *Personify* 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 www.personifysearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leica Bond Max
Good Morning, I have a question about the Leica Bond Max reagents. Has anyone ever heard of purchasing the reagents from a 3rd party rather than from Leica ? If so, what is the company's contact info. Thanks for your help. Marilynn Weber H.T.( ASCP ) QIHC Coastal Pathology Consulting Services LLC 732 814-1170 fax 267 722-8308 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 112, Issue 9
We purchased a Leica RM2125RMS manual rotary microtome new from Leica less than a year ago and you may want to be aware of the issues that we are having. We purchased it for multiple reasons, including the excellent reputation of Leica microtomes and the great sectioning of tissues (even bone) that we experienced during the demo. It still cuts great sections but I am not pleased with this purchase. We have replaced the cassette clamp at least 6 times since we've purchased it because the solid steel level to pull to open the cassette clamp breaks. Each new cassette clamp seems to last less time than the last. We are still under warranty so they have replaced this part for free every time and they are always kind when I tell them it broke again, but this is just not acceptable. We have been told that it is a known manufacturing problem and that they are working on a resolution but I would still be wary since this is obviously not fixed yet. The body of the microtome is also already showing discoloration and signs of corrosion but since these parts do not move and are not under stress it does not seem to be affecting the microtome. At this point I don't think I would ever buy Leica again, but I know that a lot of people have loved their equipment and have had very good longevity out of it. Good luck, Stephanie Weaver, HT (ASCP) From: kgrob...@rci.rutgers.edu kgrob...@rci.rutgers.edu To: histonet histonet@lists.utsouthwestern.edu Sent: Thursday, March 7, 2013 2:32 PM Subject: [Histonet] Microtome upgrade, planning stages... We are starting to look into upgrading to higher-end, but used, microtomes.? What we have is still working, but the writing is on the wall for them in terms of repair/parts. Which brands/models are considered higher-end in the used market these days?? (Leica is already on my list, but which models?) Thanks so much! Kathleen Principal Lab Technician Neurotoxicology Labs Molecular Pathology Facility Core Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] EGFRvIII
Our pathologists are requesting an antibody EGFRvIII. Is there anyone using this antibody on the Ventana Ultra or XT? Thanks in advance for your help. Billie Zimmerman MT(ASCP)QIHC 706-721-5617/3630 Augusta State University and Georgia Health Sciences University have consolidated to become Georgia Regents University. Effective January 9, 2013, my email address has changed to bzimm...@gru.edu. Please update your address book to reflect this change. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome upgrade, planning stages
I tried some different microtomes (Leica, Microm, Thermo Shandon, Sakura), as sledge as rotary types. My choise is Leica RM2245. Leica RM2245 is semi automatic? and RM2255 or RM2265 are full automatic models. I like RM2245 because this model have a rock mode, when operator does for a section only 1/4 rotation. It is very ergonomic! Shandon Finesse ME also have rock mode, but I have not happy with it. Leica RM series have only one disadvantage - two different type for disposable blade holder (for low and high profile, but not universal). Buy both type holders. RM2245 cheaper than 55 or 65. Series 2125 is the same as Microm 325. I do not try Leica Cool Clamp. Hope this help. Maxim Peshkov Russia, Taganrog. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re problem with DAB staining
Another suggestion is that your sections may have dried out sometime after the antibodies have been added and before you put on the DAB. regards steve weston lab manager resp research group MRI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Sorin-Aurelian PASCA, PhD,DVM Pathological Anatomy and Forensic Medicine Faculty of Veterinary Medicine ”University of Applied Life Sciences and Environment” 8, Al. M. Sadoveanu, 700489, Iasi Romania Mobil: 0040740174130 Office: 0040232407335 e-mail: passo...@yahoo.com spa...@uaiasi.ro ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
