RE: [Histonet] Methanol

2013-03-28 Thread Monfils, Paul
I would contact OSHA and tell them the specifics.  The possible vapor
contribution of 4 ml of methanol, if it is even measurable, is certainly
insignificant from a health/safety perspective.



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[Histonet] Expired slides

2013-03-28 Thread Dingersoll

   dingersoll@aplabo= ratories.com

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[Histonet] Re: Holes in paraffin, remedies

2013-03-28 Thread gayle callis
Hugh wrote: 

 

Sophia,

 

Holes like you mentioned, are probably from the polymers or impurities in
your embedding wax separating.  Gayle Callus mentioned this several times
(see histosearch), and recommended Stirring the embedding paraffin every
so often (before every use).  It could also be your tissue processing.
Check to make sure it is properly processed.  Embed without letting the wax
solidify before doing so.  You may have a bad batch of reagents?

 

As for the stainer, Hazy nuclei, as long as you are sure your hemotoxylin is
fine, are indicative of incomplete deparaffinzation, which could mean your
OVEN or your reagents.  It could also be your acid/base solutions.

 

Note:  Have you started a new lot # of wax?  How about xylene
(xylene-substiture) or alcohols?  I have heard people saying X-brand
paraffin was having problems with impurities that could cause these
problems.  I also heard it was solved last year. 

 

Also, your stainer has very little leeway in deparaffinzation.  You might
have to do things by hand until this problem is resolved.  You know,
deparaffinize to water using the longer method, then finish the HE in the
stainer.  Does this help?

 

 

Good luck (whoa, you have your work cut out for you),

 


***

 

Hugh, 

 

Thank you for the kind words and your reply was right on the money.In
fact, we stirred the paraffin daily before embedding, since the settling of
polymers is not just occasional.Also, clean your embedding center
frequently, before adding more paraffin.   

 

There is a clever little test for paraffin carryover into your rehydration
alcohols when removing paraffin.   1)  Use a glass beaker, add a few ml of
used alcohol  starting with last 95% just before 70% in deparaffinization
setup.   2) Pipette a few mls of  tap water into this aliquot of 95% and
look for cloudiness.   If a white cloud occurs, you have paraffin carryover
all the way down your deparaffinization process.   3) If you don't see
cloudy in this last 95%, test the 95% before this one, and keep going
backwards towards the xylene or xylene substitute.  If the next 95% is
cloudy, there is paraffin carryover.  Change out that alcohol station and
all the ones before that including the xylene/xylene substitute. 

 

Also, you can do rotation where you move the second ( closest to water)
95% into first 95% spot, then replace the second 95% with fresh, replace all
100% and xylene/xyl sub  as these latter are probably heavily contaminated
with paraffin which is carrying over into your alcohols at some level.   We
used two or three xylene/xylene substitute changes (three preferred when
doing IHC at 5 min/change) , two (or three)  100%, two 95% and one 70%
alcohol changes before distilled water, 3 minutes per change, with hand
staining.Change distilled water frequently, fresh daily and more changes
when staining many slides as you don't need alcohol carry over into your
hematoxylin.   Careful monitoring of your solvents and distilled water
should allow better hematoxylin staining. 

 

We also changed our acidic solution after hematoxylin and bluing solutions
daily, these solutions are cheap plus always doing a 1 minute running water
rinse after hematoxylin, acidic solution, and bluing.If you don't have
running water rinsing, at least change your water rinses before each
staining run.  

 

This all sounds very picky, but our HE staining was very successful.  

 

Gayle Callis

HTL/HT/MT(ASCP)  

 

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[Histonet] Validation Method for Auto HE stainer

2013-03-28 Thread Ian R Bernard
I'm looking for a benchmark or researched protocol.

IB

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[Histonet] Biocare Decloaker Nx Gen

2013-03-28 Thread Swartwood, Steven J
Has anyone ever used the Biocare Decloaking Chamber Nx Gen for epitope 
unmasking (heat retrieval)? Also does anyone have any personal pros/cons with 
this instrument? We are thinking about getting one because of inconsistencies 
with our current method using a vegetable steamer. We use many different tissue 
types both animal and human. Also we use many different antibodies. Currently 
with the vegetable steamer we've used Citrate, EDTA, and Tris-EDTA 
combinations. We've used times from 30-60 minutes, and even tried 30 minutes x 
2 or 40 minutes x 2, but we still see some inconsistent staining. To make sure 
it wasn't due to fixation vimentin staining was performed and shows even 
staining. It's just certain antibodies that are giving us trouble even from 
multiple different companies. Any advice would be greatly appreciated and very 
beneficial to our work pertaining to the instrument.

Thanks again everyone have a great end of the week,

Steven Swartwood HT(ASCP)
steven.swartw...@cshs.org

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[Histonet] RE: Biocare Decloaker Nx Gen

2013-03-28 Thread Ian R Bernard
I would welcome input hear as well since we are looking at buying one as well.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Swartwood, 
Steven J
Sent: Thursday, March 28, 2013 12:40 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Biocare Decloaker Nx Gen

Has anyone ever used the Biocare Decloaking Chamber Nx Gen for epitope 
unmasking (heat retrieval)? Also does anyone have any personal pros/cons with 
this instrument? We are thinking about getting one because of inconsistencies 
with our current method using a vegetable steamer. We use many different tissue 
types both animal and human. Also we use many different antibodies. Currently 
with the vegetable steamer we've used Citrate, EDTA, and Tris-EDTA 
combinations. We've used times from 30-60 minutes, and even tried 30 minutes x 
2 or 40 minutes x 2, but we still see some inconsistent staining. To make sure 
it wasn't due to fixation vimentin staining was performed and shows even 
staining. It's just certain antibodies that are giving us trouble even from 
multiple different companies. Any advice would be greatly appreciated and very 
beneficial to our work pertaining to the instrument.

Thanks again everyone have a great end of the week,

Steven Swartwood HT(ASCP)
steven.swartw...@cshs.org

IMPORTANT WARNING: This message is intended for the use of the person or entity 
to which it is addressed and may contain information that is privileged and 
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reader of this message is not the intended recipient, or the employee or agent 
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notified that any dissemination, distribution or copying of this information is 
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[Histonet] RE: Biocare Decloaker Nx Gen

2013-03-28 Thread Morken, Timothy
We tried the Decloaking Chamber Plus a couple years ago after using the 
Decloaking chamber for years. The Decloaking chamber gave us great results, but 
the record keeping (time, temp, pressure) for four Decloakers was time 
consuming and irritating. The PLUS model uses a USB stick to record all that so 
we didn't have to. A great improvement.  But we ended up going with Bond 
stainers which obviated all that.

However, it was a good upgrade, and the NX looks even better - Pre-programmed, 
easier to use, and with the USB feature. 

Pressure cookers and waterbaths generally give more consistent results than 
steamers since there is variation in the heating within a steam chamber so I 
think it will help you a lot to change over. With the new instruments' 
automation it should be painless to operate. 

Tim Morken
UCSF Medical Center
Pathology Dept

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ian R Bernard
Sent: Thursday, March 28, 2013 10:44 AM
To: Swartwood, Steven J; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Biocare Decloaker Nx Gen

I would welcome input hear as well since we are looking at buying one as well.

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Swartwood, 
Steven J
Sent: Thursday, March 28, 2013 12:40 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Biocare Decloaker Nx Gen

Has anyone ever used the Biocare Decloaking Chamber Nx Gen for epitope 
unmasking (heat retrieval)? Also does anyone have any personal pros/cons with 
this instrument? We are thinking about getting one because of inconsistencies 
with our current method using a vegetable steamer. We use many different tissue 
types both animal and human. Also we use many different antibodies. Currently 
with the vegetable steamer we've used Citrate, EDTA, and Tris-EDTA 
combinations. We've used times from 30-60 minutes, and even tried 30 minutes x 
2 or 40 minutes x 2, but we still see some inconsistent staining. To make sure 
it wasn't due to fixation vimentin staining was performed and shows even 
staining. It's just certain antibodies that are giving us trouble even from 
multiple different companies. Any advice would be greatly appreciated and very 
beneficial to our work pertaining to the instrument.

Thanks again everyone have a great end of the week,

Steven Swartwood HT(ASCP)
steven.swartw...@cshs.org

IMPORTANT WARNING: This message is intended for the use of the person or entity 
to which it is addressed and may contain information that is privileged and 
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reader of this message is not the intended recipient, or the employee or agent 
responsible for delivering it to the intended recipient, you are hereby 
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[Histonet] List of Specimens a PA can Gross

2013-03-28 Thread Wanda.Smith
Hello All,
CAP IS HERE AS I TYPE THIS, and I am looking for a copy of the policy regarding 
what specimens a PA can gross with and without direct supervision.  Will 
someone share theirs with me?  I am aware every lab is different, but it 
keep me from being cited!
Thanks,
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or 
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[Histonet] RE: Biocare Decloaker Nx Gen

2013-03-28 Thread Blazek, Linda
We have used the Decloaking Chamber for several years now and have had 
excellent results. The nicest thing is that there is consistency and 
reliability.  Each run will be the same as the one previous and having the 
ability to use the USB stick for documentation of time, pressure and 
temperature makes record keeping really easy.  We have had no complaints.  The 
only con I can come up with is that it comes in a big box that is hard to store 
if you have to send it back for some reason.

Linda Blazek HT (ASCP)
Manager/Supervisor
GI Pathology of Dayton
Digestive Specialists, Inc
Phone: (937) 396-2623
Email: lbla...@digestivespecialists.com




-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Swartwood, 
Steven J
Sent: Thursday, March 28, 2013 1:40 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Biocare Decloaker Nx Gen

Has anyone ever used the Biocare Decloaking Chamber Nx Gen for epitope 
unmasking (heat retrieval)? Also does anyone have any personal pros/cons with 
this instrument? We are thinking about getting one because of inconsistencies 
with our current method using a vegetable steamer. We use many different tissue 
types both animal and human. Also we use many different antibodies. Currently 
with the vegetable steamer we've used Citrate, EDTA, and Tris-EDTA 
combinations. We've used times from 30-60 minutes, and even tried 30 minutes x 
2 or 40 minutes x 2, but we still see some inconsistent staining. To make sure 
it wasn't due to fixation vimentin staining was performed and shows even 
staining. It's just certain antibodies that are giving us trouble even from 
multiple different companies. Any advice would be greatly appreciated and very 
beneficial to our work pertaining to the instrument.

Thanks again everyone have a great end of the week,

Steven Swartwood HT(ASCP)
steven.swartw...@cshs.org

IMPORTANT WARNING: This message is intended for the use of the person or entity 
to which it is addressed and may contain information that is privileged and 
confidential, the disclosure of which is governed by applicable law. If the 
reader of this message is not the intended recipient, or the employee or agent 
responsible for delivering it to the intended recipient, you are hereby 
notified that any dissemination, distribution or copying of this information is 
STRICTLY PROHIBITED. If you have received this message in error, please notify 
us immediately by calling (310) 423-6428 and destroy the related message. Thank 
You for your cooperation.
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[Histonet] Updates on Jekyll Island...Procrastinators please read this ASAP

2013-03-28 Thread Zimmerman, Billie
If you haven't reserved your room for the upcoming GSH meeting next month, 
please note that all island and standard Oceanside rooms have been reserved.  
But,  just because you snoozed, were waiting on money for your institution, or 
you're just a classic procrastinator, there are still options.  There are 
upgraded rooms such as the lanai.  This room has a Jacuzzi, microwave, King 
size bed, small refrigerator, and a private balcony or patio. There's also the 
efficiency which has the kitchenette with a stove top, refrigerator,  two 
double beds, sitting area, and private balcony or patio.  The symposium rate 
still applies and if you have issues or concerns, contact Linda Schepps at 
Oceanside.  She can't cure procrastination but she can help secure a room for 
you.

While you're strolling around the historic area of the island in your speedo or 
crochet bikini, check out Becky's famous chicken salad.  It's a little place 
with outside tables.  Just walk up to the window and place your order.  I 
attempted to have a chicken salad sandwich there but they were all sold out!! 
The lady managed to let me sample a teaspoon of it. It was delicious and didn't 
taste like that stuff in the grocery store.  I plan to attempt another order 
when I return next month!!


Augusta State University and Georgia Health Sciences University have 
consolidated to become Georgia Regents University. Effective January 9, 2013, 
my email address has changed to bzimm...@gru.edu. Please update your address 
book to reflect this change.
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RE: [Histonet] Updates on Jekyll Island...Procrastinators please read this ASAP

2013-03-28 Thread Jack Ratliff
PLEASE! PLEASE! PLEASE! I want that room! 
Oh and since you mentioned speedos and crochet bikinis, you want me to bring my 
white mankini with the British flag on the front? Just kidding, I think I had 
better leave that at home! 
Jack

Jack L. RatliffOwner/Histologist, Ratliff Histology Consultants, LLCChairman, 
Hard Tissue Committee - National Society for Histotechnology
389 Nichol Mill LaneFranklin, TN 37067(317) 281-1975 (c)(615) 236-4901 (o)(615) 
236-4962 (f)jratl...@ratliffhistology.com


 From: bzimm...@gru.edu
 To: histonet@lists.utsouthwestern.edu
 Date: Thu, 28 Mar 2013 18:57:46 +
 Subject: [Histonet] Updates on Jekyll Island...Procrastinators please read
 this ASAP
 
 If you haven't reserved your room for the upcoming GSH meeting next month, 
 please note that all island and standard Oceanside rooms have been reserved.  
 But,  just because you snoozed, were waiting on money for your institution, 
 or you're just a classic procrastinator, there are still options.  There are 
 upgraded rooms such as the lanai.  This room has a Jacuzzi, microwave, King 
 size bed, small refrigerator, and a private balcony or patio. There's also 
 the efficiency which has the kitchenette with a stove top, refrigerator,  two 
 double beds, sitting area, and private balcony or patio.  The symposium rate 
 still applies and if you have issues or concerns, contact Linda Schepps at 
 Oceanside.  She can't cure procrastination but she can help secure a room for 
 you.
 
 While you're strolling around the historic area of the island in your speedo 
 or crochet bikini, check out Becky's famous chicken salad.  It's a little 
 place with outside tables.  Just walk up to the window and place your order.  
 I attempted to have a chicken salad sandwich there but they were all sold 
 out!! The lady managed to let me sample a teaspoon of it. It was delicious 
 and didn't taste like that stuff in the grocery store.  I plan to attempt 
 another order when I return next month!!
 
 
 Augusta State University and Georgia Health Sciences University have 
 consolidated to become Georgia Regents University. Effective January 9, 2013, 
 my email address has changed to bzimm...@gru.edu. Please update your address 
 book to reflect this change.
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[Histonet] RE: Biocare Decloaker Nx Gen

2013-03-28 Thread Tiana Baskin
I have had the pleasure of seeing a demo of these Nxgen decloakers and I was 
very impressed. I currently use the older generation ones which still work very 
reliably for me. There is a nice tray inside the chamber to ensure the slide 
containers do not tip over and the USB which tracks all of the temperature and 
pressure information is a QC dream. The only thing which I could see as a down 
side would be the programming. It is preset from what I remember so you have to 
choose from the programs already existing. I think the existing programs should 
cover most antigens but if you wanted to be creative you don't have that 
option. I would love to have one in my lab ... Enjoy
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[Histonet] (no subject)

2013-03-28 Thread Jennifer Blanchard

http://www.savvybusinessmakers.com/iseiy/pqih.yxdxy?eglxo  


 



 
3/28/2013 8:03:09 PM



 

 
  

 













3/28/2013 8:03:09 PM
   
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[Histonet] Thanks for your help on PA Grossing

2013-03-28 Thread Wanda.Smith
Thanks to ALL!
I have what I need!!!
Wanda

WANDA G. SMITH, HTL(ASCP)HT
Pathology Supervisor
TRIDENT MEDICAL CENTER
9330 Medical Plaza Drive
Charleston, SC  29406
843-847-4586
843-847-4296 fax

This email and any files transmitted with it may contain PRIVILEGED or 
CONFIDENTIAL information and may be read or used only by the intended 
recipient. If you are not the intended recipient of the email or any of its 
attachments, please be advised that you have received this email in error and 
that any use, dissemination, distribution, forwarding, printing, or copying of 
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the sender by reply email or contact the sender at the number listed.

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[Histonet] Updates on Jekyll Island

2013-03-28 Thread Shirley A. Powell
Waaay too much information Jack, just keep it covered.  You can 
bring all those wonderful photos of your European trip with your Dad.  I do 
hope you have made your reservations at Jekyll.  Sounds like the rooms are 
going fast.  

See you at the beach.  Oh and Jack, they will arrest you if you show too much.  
This is Georgia you know.  

Sp

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff
Sent: Thursday, March 28, 2013 3:17 PM
To: Zimmerman, Billie; Histonet
Subject: RE: [Histonet] Updates on Jekyll Island...Procrastinators please read 
this ASAP

PLEASE! PLEASE! PLEASE! I want that room! 
Oh and since you mentioned speedos and crochet bikinis, you want me to bring my 
white mankini with the British flag on the front? Just kidding, I think I had 
better leave that at home! 
Jack

Jack L. RatliffOwner/Histologist, Ratliff Histology Consultants, LLCChairman, 
Hard Tissue Committee - National Society for Histotechnology
389 Nichol Mill LaneFranklin, TN 37067(317) 281-1975 (c)(615) 236-4901 (o)(615) 
236-4962 (f)jratl...@ratliffhistology.com


 From: bzimm...@gru.edu
 To: histonet@lists.utsouthwestern.edu
 Date: Thu, 28 Mar 2013 18:57:46 +
 Subject: [Histonet] Updates on Jekyll Island...Procrastinators please read
 this ASAP
 
 If you haven't reserved your room for the upcoming GSH meeting next month, 
 please note that all island and standard Oceanside rooms have been reserved.  
 But,  just because you snoozed, were waiting on money for your institution, 
 or you're just a classic procrastinator, there are still options.  There are 
 upgraded rooms such as the lanai.  This room has a Jacuzzi, microwave, King 
 size bed, small refrigerator, and a private balcony or patio. There's also 
 the efficiency which has the kitchenette with a stove top, refrigerator,  two 
 double beds, sitting area, and private balcony or patio.  The symposium rate 
 still applies and if you have issues or concerns, contact Linda Schepps at 
 Oceanside.  She can't cure procrastination but she can help secure a room for 
 you.
 
 While you're strolling around the historic area of the island in your speedo 
 or crochet bikini, check out Becky's famous chicken salad.  It's a little 
 place with outside tables.  Just walk up to the window and place your order.  
 I attempted to have a chicken salad sandwich there but they were all sold 
 out!! The lady managed to let me sample a teaspoon of it. It was delicious 
 and didn't taste like that stuff in the grocery store.  I plan to attempt 
 another order when I return next month!!
 
 
 Augusta State University and Georgia Health Sciences University have 
 consolidated to become Georgia Regents University. Effective January 9, 2013, 
 my email address has changed to bzimm...@gru.edu. Please update your address 
 book to reflect this change.
 ___
 Histonet mailing list
 Histonet@lists.utsouthwestern.edu
 http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  
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[Histonet] RE: Updates on Jekyll Island

2013-03-28 Thread Jack Ratliff
Im not that crazy Shirley, but I have worn a speedo on the beach within the 
last 8 months just for fun! LOL
Yes, I have my reservation and I believe it is ocean/beach side, but that 
jacuzzi is a tempting thought to change! My Dad always says that only a fool 
wouldn't change their mind! LOL
One more thing, if the weather is nice during those days, I'm seriously 
thinking of riding my Harley out there!
See you all very soon!
Jack


 From: powell...@mercer.edu
 To: ratliffj...@hotmail.com; bzimm...@gru.edu; 
 histonet@lists.utsouthwestern.edu
 Date: Thu, 28 Mar 2013 16:15:51 -0400
 Subject: Updates on Jekyll Island
 
 Waaay too much information Jack, just keep it covered.  You 
 can bring all those wonderful photos of your European trip with your Dad.  I 
 do hope you have made your reservations at Jekyll.  Sounds like the rooms are 
 going fast.  
 
 See you at the beach.  Oh and Jack, they will arrest you if you show too 
 much.  This is Georgia you know.  
 
 Sp
 
 -Original Message-
 From: histonet-boun...@lists.utsouthwestern.edu 
 [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jack Ratliff
 Sent: Thursday, March 28, 2013 3:17 PM
 To: Zimmerman, Billie; Histonet
 Subject: RE: [Histonet] Updates on Jekyll Island...Procrastinators please 
 read this ASAP
 
 PLEASE! PLEASE! PLEASE! I want that room! 
 Oh and since you mentioned speedos and crochet bikinis, you want me to bring 
 my white mankini with the British flag on the front? Just kidding, I think I 
 had better leave that at home! 
 Jack
 
 Jack L. RatliffOwner/Histologist, Ratliff Histology Consultants, LLCChairman, 
 Hard Tissue Committee - National Society for Histotechnology
 389 Nichol Mill LaneFranklin, TN 37067(317) 281-1975 (c)(615) 236-4901 
 (o)(615) 236-4962 (f)jratl...@ratliffhistology.com
 
 
  From: bzimm...@gru.edu
  To: histonet@lists.utsouthwestern.edu
  Date: Thu, 28 Mar 2013 18:57:46 +
  Subject: [Histonet] Updates on Jekyll Island...Procrastinators please read  
  this ASAP
  
  If you haven't reserved your room for the upcoming GSH meeting next month, 
  please note that all island and standard Oceanside rooms have been 
  reserved.  But,  just because you snoozed, were waiting on money for your 
  institution, or you're just a classic procrastinator, there are still 
  options.  There are upgraded rooms such as the lanai.  This room has a 
  Jacuzzi, microwave, King size bed, small refrigerator, and a private 
  balcony or patio. There's also the efficiency which has the kitchenette 
  with a stove top, refrigerator,  two double beds, sitting area, and private 
  balcony or patio.  The symposium rate still applies and if you have issues 
  or concerns, contact Linda Schepps at Oceanside.  She can't cure 
  procrastination but she can help secure a room for you.
  
  While you're strolling around the historic area of the island in your 
  speedo or crochet bikini, check out Becky's famous chicken salad.  It's a 
  little place with outside tables.  Just walk up to the window and place 
  your order.  I attempted to have a chicken salad sandwich there but they 
  were all sold out!! The lady managed to let me sample a teaspoon of it. It 
  was delicious and didn't taste like that stuff in the grocery store.  I 
  plan to attempt another order when I return next month!!
  
  
  Augusta State University and Georgia Health Sciences University have 
  consolidated to become Georgia Regents University. Effective January 9, 
  2013, my email address has changed to bzimm...@gru.edu. Please update your 
  address book to reflect this change.
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