[Histonet] staffing

2013-05-14 Thread Laurie Best
Hi All,
Does anyone have information regarding the correct ratio of Histotechs to 
Patholgists? I seem to remember getting that info at an NSH convention, but I 
cannot locate it.
Thanks ahead of time.
Laurie

Laurie Best
Histology Supervisor
Sunrise Medical Laboratories
240 Miller Place
Hiscksville, N.Y. 11801
tel (631)435-1515x1018
fax(631)435-2014

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Re: [Histonet] staffing

2013-05-14 Thread Rene J Buesa
There is no correct ratio for HT to PT. That depends on the way the director 
of pathology runs the practice, the volume of work and the automation condition 
of the lab.
You can go to http://www.histosearch.com/rene.html and read an article I wrote 
on staffing where you can find answer to your question.
René J.

From: Laurie Best lb...@sunriselab.com
To: 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu 
Sent: Tuesday, May 14, 2013 7:46 AM
Subject: [Histonet] staffing


Hi All,
Does anyone have information regarding the correct ratio of Histotechs to 
Patholgists? I seem to remember getting that info at an NSH convention, but I 
cannot locate it.
Thanks ahead of time.
Laurie

Laurie Best
Histology Supervisor
Sunrise Medical Laboratories
240 Miller Place
Hiscksville, N.Y. 11801
tel (631)435-1515x1018
fax(631)435-2014

This message contains privileged and confidential information intended only 
for the use of the addressee named above. If you are not the intended recipient 
of this message you must not disseminate, copy or take any action in reliance 
on it.

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[Histonet] Formula 83: xylene substitute

2013-05-14 Thread Kiran
Hello Everyone,

I would like to get some feedback from Formula 83 users. Are you using it only 
on the stainers or for tissue processing as well. What kind of validation 
process is required to switch to this substitute for ihc stains? We are 
interested to use it for both histo and cyto lab.
Any input is much appreciated.

Thank you in advance,

Kiran
Sent from my iPad
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RE: [Histonet] Formula 83: xylene substitute

2013-05-14 Thread Blazek, Linda
Kiran,
We use Formula 83 on both staining and processing without any problem at all.  
You have to watch and either rotate or change your last Formula 83s on the 
stain line because you can't tell if there is water in it like you can with 
xylene.  We also use CBG's recycler to recycle Formula 83 without any problem.  
We have not had any problems with our IHC using Formula 83 either.  

Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kiran
Sent: Tuesday, May 14, 2013 9:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formula 83: xylene substitute

Hello Everyone,

I would like to get some feedback from Formula 83 users. Are you using it only 
on the stainers or for tissue processing as well. What kind of validation 
process is required to switch to this substitute for ihc stains? We are 
interested to use it for both histo and cyto lab.
Any input is much appreciated.

Thank you in advance,

Kiran
Sent from my iPad
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[Histonet] Histology Technician Opening.

2013-05-14 Thread Podawiltz, Thomas
As of May 24th I will have an opening for a Tech. Days only 40 hours per week. 
No weekends doing routine histology, no IHC.
Located in the beautiful Lakes Region of New Hampshire.

* If interested here is the link: LRGHealthcare | Career 
Centerhttp://www.lrgh.org/Jobs
www.lrgh.org/Jobs


Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer.
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220

THIS MESSAGE IS CONFIDENTIAL.  
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please notify the sender by return e-mail and delete this message and any 
attachments from your computer. Any views or opinions expressed are solely 
those of the author and do not necessarily represent those of LRGHealthcare.
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[Histonet] RELIA Histology Careers Bulletin. 5-14-2013. Exciting New Job Opportunities. I hope you had a nice Mother's Day!!

2013-05-14 Thread Pam Barker
Hi Histonetters!!
I hope you had a lovely Mother's Day!!!
Can you believe it?  Summer is almost here!!!  
My phone has been ringing off the hook this past week with some exciting new
opportunities.  
This is a quick update of the positions I am working on that I am most
excited about.  Some are brand new and some are additional positions where I
have placed people who love their new jobs.  As you know all of the
positions I work with are full-time permanent positions with the best
hospitals, labs and clinics.  I only work with clients that offer excellent
compensation including competitive salaries, great benefits and relocation
assistance /sign on bonuses.
 
Spotlight Opportunities:
Mohs Tech - Phoenix, AZ -busy growing lab!
Lead Histotechnologist - Covington, KY - brand new lab!!!
Histology Tech - Tyler, TX - Busy Hospital Lab beautiful area!!
 
Here is a list of the rest of the positions I am most excited to tell you
about:
 
Charlotte, NC:   HT/HTL Nights 10K sign on, great benefits.
Long Beach, CA:   Mohs Tech with HT/HTL, Brand New Lab 
Chattanooga, TN: Grossing HistoTech Nights Possible lead 
Zanesville, OH: Histotech - Busy Lab IHC experience preferred
Paterson, NJ: Histotech Nights - Brand New Lab
Tyler, TX:  Histotech Days- Busy Hospital Lab
Syracuse, NY - Histotech Days NY lic, great lab and team to work with!!
 
If you are interested in any of these positions please call me at
866-607-3542 or e-mail me at rel...@earthlink.net  with your resume and a
number where I can reach you at a time that is convenient for you.  If you
are interested in looking into new job opportunities in other areas that are
not mentioned above please contact me as well.  I work with facilities
nationwide and I will keep your resume confidential.  I will only represent
you to jobs you tell me you are interested in looking into.
 
Remember. It never hurts to keep an eye open even if you are happy in your
present job.
 
Also if you know anyone else that might be interested I would really
appreciate it if you would pass my information along to them as well.  If I
place someone you refer you will earn a referral fee.  
 
Thanks-Pam


Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com http://www.facebook.com/PamBarkerRELIA /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 





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Fw: [Histonet] p40

2013-05-14 Thread Akemi Allison
Hey Jim:
 
Just read the abstract you sent the link too.  Once again congratulations on 
yet another accepted abstract!  That was pretty much the jest of Davids' 
information and side-by-side images of p63 verses p40 on lung CA.
 
Akemi Allison B.S., HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
(381) 375-3577  X117
Email: aalli...@montereygi.com


 



From: Jim Burchette jburc...@gmail.com
To: Akemi Allison akemiat3...@yahoo.com 
Cc: Taylor, Jean jtay...@meriter.com; ih...@googlegroups.com 
ih...@googlegroups.com 
Sent: Monday, May 13, 2013 3:44 PM
Subject: Re: [Histonet] p40



Another application for p40:

p40 (ΔNp63) and keratin 34βE12 provide greater diagnostic accuracy than p63 in 
the evaluation of small cell lung carcinoma in small biopsy samples.
Butnor KJ, Burchette JL.
Hum Pathol. 2013 Mar 22. doi:pii: S0046-8177(13)00044-0. 
10.1016/j.humpath.2013.01.011. [Epub ahead of print]


On Fri, May 10, 2013 at 6:35 PM, Akemi Allison akemiat3...@yahoo.com wrote:

Biocare Medical. David Tacha just gave a a workshop on this at the CSH meeting 
last weekend.
 
Akemi Allison BS, HT(ASCP)HTL
Director
Phoenix Lab Consulting
E-Mail: akemiat3...@yahoo.com





 From: Taylor, Jean jtay...@meriter.com
To: 'ih...@googlegroups.com' ih...@googlegroups.com; 
'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu
Sent: Friday, May 10, 2013 10:53 AM
Subject: [Histonet] p40



Hi everyone,

I'm looking into ordering the p40 antibody for one of our pathologists. He 
wants to use it to help distinguish between adenocarcinoma and squamous cell 
ca in lung. I'm wondering what labs are using, the monoclonal or polyclonal 
antibody, and where you purchase it from. I haven't found that a lot of 
companies that carry it. Any info would be helpful.

Thanks,

Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
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-- 
Jim Burchette
Fly Fishing Bum  '(((
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[Histonet] Lab Equipment: suggestions please

2013-05-14 Thread Jb
Any suggestions on a good microtome?
Cassette printer, slide printer?  Need to purchase and would love to hear 
suggestions of what's good  bad products to buy. 

Thank you

Sent from my iPhone
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[Histonet] Gross Room cleaner

2013-05-14 Thread Amber McKenzie

What do you guys use to clean your Gross Room utensils?  I bought Lem'in Lift 
from Mantek in the past to soak the rulers, scissors, tweezers, etc...Thanks!

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[Histonet] Lysol IC for Ventana equipmetn cleaner

2013-05-14 Thread Amber McKenzie

I used to use Amphyl to decon my Ventana equipment, but now Cardinal sells 
Lysol IC in its place and I was wondering how you guys make it up...water to 
cleaner ratio in the Vantana carboys. With Amphyl I did 20L dH20/200ml Amphyl.  
Thanks! 

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RE: [Histonet] Formula 83: xylene substitute

2013-05-14 Thread Kiranjit Grewal
Thanks, Linda. 
 
What about the odor? Does it have strong or irritating odor? How did you staff 
responded to the change? We would like to know all before trying it out. 
Currenly, our staff is having issues with Xylene fumes and goal is to limit any 
fumes or exposure to Xylene.
 
I would like to hear from others who tried to use it and didn't work for them.
 
-Kiran

--- On Tue, 5/14/13, Blazek, Linda lbla...@digestivespecialists.com wrote:


From: Blazek, Linda lbla...@digestivespecialists.com
Subject: RE: [Histonet] Formula 83: xylene substitute
To: Kiran kira...@sbcglobal.net, histonet@lists.utsouthwestern.edu 
histonet@lists.utsouthwestern.edu
Date: Tuesday, May 14, 2013, 7:13 AM


Kiran,
We use Formula 83 on both staining and processing without any problem at all.  
You have to watch and either rotate or change your last Formula 83s on the 
stain line because you can't tell if there is water in it like you can with 
xylene.  We also use CBG's recycler to recycle Formula 83 without any problem.  
We have not had any problems with our IHC using Formula 83 either.  

Linda

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kiran
Sent: Tuesday, May 14, 2013 9:29 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Formula 83: xylene substitute

Hello Everyone,

I would like to get some feedback from Formula 83 users. Are you using it only 
on the stainers or for tissue processing as well. What kind of validation 
process is required to switch to this substitute for ihc stains? We are 
interested to use it for both histo and cyto lab.
Any input is much appreciated.

Thank you in advance,

Kiran
Sent from my iPad
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[Histonet] RE: Lysol IC for Ventana equipmetn cleaner

2013-05-14 Thread Tom McNemar
We use it but we only make it up 1 gallon at a time.  We use 20ml to 1 gallon 
of distilled per Ventana.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org
www.LMHealth.org


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Tuesday, May 14, 2013 12:33 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Lysol IC for Ventana equipmetn cleaner


I used to use Amphyl to decon my Ventana equipment, but now Cardinal sells 
Lysol IC in its place and I was wondering how you guys make it up...water to 
cleaner ratio in the Vantana carboys. With Amphyl I did 20L dH20/200ml Amphyl.  
Thanks!

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[Histonet] Re: [IHCRG] p40

2013-05-14 Thread Richard Cartun
I have been very impressed with BioCare's p40 monoclonal predilute. 
We are able to use it a 1:5 dilution on our Bond Max platform. 
Excellent immunoreactivity so far!
 
Richard


Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


 Taylor, Jean jtay...@meriter.com 5/10/2013 1:53 PM 

Hi everyone,
 
I’m looking into ordering the p40 antibody for one of our pathologists.
He wants to use it to help distinguish between adenocarcinoma and
squamous cell ca in lung. I’m wondering what labs are using, the
monoclonal or polyclonal antibody, and where you purchase it from. I
haven’t found that a lot of companies that carry it. Any info would be
helpful.
 
Thanks,
 
Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI

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To contact the National Society for Histotechnology, email:
hi...@nsh.org or call 443.535.4060.
 
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[Histonet] RE: Gross Room cleaner

2013-05-14 Thread Sarah Dysart
Bleach =)
Just make sure to rinse after cleaning or they will rust

Sarah Goebel-Dysart, BA, HT(ASCP), QIHC (ASCP)
Histotechnologist
Mirna Therapeutics
2150 Woodward Street
Suite 100
Austin, Texas  78744
(512)901-0900 ext. 6912


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Tuesday, May 14, 2013 11:29 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Gross Room cleaner


What do you guys use to clean your Gross Room utensils?  I bought Lem'in Lift 
from Mantek in the past to soak the rulers, scissors, tweezers, etc...Thanks!

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RE: [Histonet] Re: [IHCRG] p40

2013-05-14 Thread Patsy Ruegg
Richard now the ab companies are really going to hate you advocating diluting 
predilute's further isn't that a sacrilege, what are we going to do with you, 
no negs and now diluting predilutes.  I am just kidding I do it all the time 
myself. 
Cheers, Patsy

Date: Tue, 14 May 2013 13:33:21 -0400
From: rcar...@harthosp.org
To: ih...@googlegroups.com; histonet@lists.utsouthwestern.edu; 
jtay...@meriter.com
CC: 
Subject: [Histonet] Re: [IHCRG] p40

I have been very impressed with BioCare's p40 monoclonal predilute. 
We are able to use it a 1:5 dilution on our Bond Max platform. 
Excellent immunoreactivity so far!
 
Richard
 
 
Richard W. Cartun, MS, PhD
Director, Histology  Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax
 
 
 Taylor, Jean jtay...@meriter.com 5/10/2013 1:53 PM 
 
Hi everyone,
 
I’m looking into ordering the p40 antibody for one of our pathologists.
He wants to use it to help distinguish between adenocarcinoma and
squamous cell ca in lung. I’m wondering what labs are using, the
monoclonal or polyclonal antibody, and where you purchase it from. I
haven’t found that a lot of companies that carry it. Any info would be
helpful.
 
Thanks,
 
Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI
 
-- 
-- 
You received this message because you are subscribed to the Google
Groups ihcrg group. The IHC Resource Group is a standing committee
within the National Society for Histotechnology.
 
To post to this group, send email to ih...@googlegroups.com
To unsubscribe from this group, send email to
ihcrg+unsubscr...@googlegroups.com
For more options, visit this group at
http://groups.google.com/group/ihcrg?hl=en
 
To contact the National Society for Histotechnology, email:
hi...@nsh.org or call 443.535.4060.
 
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[Histonet] Bone marrow aspirate iron stain

2013-05-14 Thread Renz, Jason
Hello, We are currently having problems with our bone marrow aspirate slides 
for iron stains which we run on the Ventana nexes special stainer. The RBCs 
look crenated or damaged. We currently fix them 5 minutes in methanol then air 
dry before running them on the stainer. Any suggestions?
Jason Renz
HT(ASCP)/lab safety officer
St. Alexius Medical Center
900 East Broadway
Bismarck ND, 58506
(701)530-6733


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[Histonet] Gomori Trichrome Staining

2013-05-14 Thread Eleonora Guadagnin
Hello everybody,
I am performing the Gomori Trichrome staining on frozen muscle sections
(10um) but I have couple of issues:
1. The green staining is TOO dark;
2. When the green is not too dark, all my muscle fibers are red.

I checked the pH of the Gomori solution, is 2.15.

If anyone has some advice, please
Thanks a lot!

Eleonora
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Re: [Histonet] Gross Room cleaner

2013-05-14 Thread Paula Sicurello
Dispatch works well too.

Paula
-- 
Paula Sicurello, HTL (ASCP)
Supervisor, Clinical Electron Microscopy Laboratory
Duke University Health System
Rm.#251M, Duke South, Green Zone
Durham, North Carolina 27710
P:  919.684.2091

On Tue, May 14, 2013 at 12:28 PM, Amber McKenzie 
amber.mcken...@gastrodocs.net wrote:


 What do you guys use to clean your Gross Room utensils?  I bought Lem'in
 Lift from Mantek in the past to soak the rulers, scissors, tweezers,
 etc...Thanks!

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[Histonet] RE: Bone marrow aspirate iron stain

2013-05-14 Thread Tony Henwood (SCHN)
Hi Jason,
I am not familiar with the stainer but is the procedure starting from water 
(not dewaxing  re-hydration as is used for paraffin sections)?

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager  Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Renz, Jason
Sent: Wednesday, 15 May 2013 5:07 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Bone marrow aspirate iron stain

Hello, We are currently having problems with our bone marrow aspirate slides 
for iron stains which we run on the Ventana nexes special stainer. The RBCs 
look crenated or damaged. We currently fix them 5 minutes in methanol then air 
dry before running them on the stainer. Any suggestions?
Jason Renz
HT(ASCP)/lab safety officer
St. Alexius Medical Center
900 East Broadway
Bismarck ND, 58506
(701)530-6733


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[Histonet] RE: Lysol IC for Ventana equipmetn cleaner

2013-05-14 Thread Sebree Linda A
The dilution is listed on the bottle...I'm not at work but its something like 
1:252.

Linda A. Sebree

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] on behalf of Amber McKenzie 
[amber.mcken...@gastrodocs.net]
Sent: Tuesday, May 14, 2013 11:33 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Lysol IC for Ventana equipmetn cleaner

I used to use Amphyl to decon my Ventana equipment, but now Cardinal sells 
Lysol IC in its place and I was wondering how you guys make it up...water to 
cleaner ratio in the Vantana carboys. With Amphyl I did 20L dH20/200ml Amphyl.  
Thanks!

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[Histonet] HPV In situ hybridization protocol (FISH or CISH)

2013-05-14 Thread Mesru T
Dear Histonetters,

Does anybody have a protocol for HPV in situ hybridization (preferably high
risk type probes). Preferably on ThinPrep cytological samples or similar.

Regards,
Mesru Turkekul
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[Histonet] Mohs histology hourly rate

2013-05-14 Thread rook570
Hello, I was wondering if anyone knows what the going rate is for a Mohs 
histologist (Hourly). I have 12 years experience as a histologist doing routine 
histology and just recently got a job doing Mohs histology. Thanks in advance 
for any feed back, Christina 
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Re: [Histonet] Mohs histology hourly rate

2013-05-14 Thread Will Chappell
Location matters. Where?

Sent from my iPhone

On May 14, 2013, at 6:10 PM, rook...@comcast.net wrote:

 Hello, I was wondering if anyone knows what the going rate is for a Mohs 
 histologist (Hourly). I have 12 years experience as a histologist doing 
 routine histology and just recently got a job doing Mohs histology. Thanks in 
 advance for any feed back, Christina 
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 Histonet@lists.utsouthwestern.edu
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