[Histonet] hsp70
Hello I'm new to this but here goes... Does anyone have HSP70 worked up and if so what is your methodology? I am trying to work it up on hepatacellular carcinoma using Santa Cruz antibody. Thanks for any input. Michaela LeFaivre BS, HTL (ASCP) CM Molecular Technician III Molecular Pathology Rm 4344 Purple Zone 919-684-4303 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PAS D
Hello eveyone I was just wondering who does PAS D and would you please share your procedure with me, it been a while since I ve done this stain. Thank you in advance Madeline Rotger Milanese H.T. BSHCS 500 New Hempstead Rd. New City N.Y. 10965 845-362-3200 Ext 129 madelin...@yahoo.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] bone marrow aspirate slides for iron stains
Try hydrating the slide in 95% then distilled before staining. The iron stain is made in water so hydrating to water should help the stain transfer better. Hope this helps :) Cassandra Davis cda...@che-east.org 302-575-8095 Saint Francis Hospital Saintfrancishealthcare.org Saint Francis Facebook Page Confidentiality Notice: This e-mail, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] fungal stain for frozen sections
Hi, I work in a Surgical Pathology frozen section lab outside the OR rooms. Normally we only perform an HE on the frozens. Now we are looking for a fast and inexpensive stain for fungus on our frozen sections. Fast is the operative word because we have to report diagnoses back to ORs in a 20 minute timeperiod. Any ideas to help? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] fungal stain for frozen sections
It appears, from a research paper I was reading, that a 1% solution of uvitex 2b added to eosin will cause fungus to fluoresce under uv light. I haven't performed it, however it could be as quick as an HE stain. Looks promising. Http://rd.springer.com/article/10.1007%2FBF02889994#page-1 Will Chappell CHOC children's hospital. Sent from my iPhone On May 16, 2013, at 9:00 AM, wrg...@aol.com wrote: Hi, I work in a Surgical Pathology frozen section lab outside the OR rooms. Normally we only perform an HE on the frozens. Now we are looking for a fast and inexpensive stain for fungus on our frozen sections. Fast is the operative word because we have to report diagnoses back to ORs in a 20 minute timeperiod. Any ideas to help? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] fungal stain for frozen sections
span style=font-size:10pt;p style=margin-top:0;margin-bottom:0;At what wave length? nbsp;Eosin nbsp;is is also fluorescentnbsp;/pp style=margin-top:0;margin-bottom:0;nbsp;/pdev3_jjySent from my Boost Mobile phone./dev3_jjy pnbsp;/pfontpnbsp;/ppnbsp;/p-- Original Message --brbFrom:nbsp;/bWill Chappells cha...@yahoo.combrbDate:nbsp;/b5/16/2013 12:18 PMbrbTo:nbsp;/bwrg...@aol.com;brbCc:nbsp;/bhistonet@lists.utsouthwestern.edu;brbSubject:nbsp;/bRe: [Histonet] fungal stain for frozen sectionspnbsp;/ppreIt appears, from a research paper I was reading, that a 1% solution of uvitex 2b added to eosin will cause fungus to fluoresce under uv light. I haven't performed it, however it could be as quick as an Hamp;E stain. Looks promising. Http://rd.springer.com/article/10.1007%2FBF02889994#page-1 Will Chappell CHOC children's hospital. Sent from my iPhone On May 16, 2013, at 9:00 AM, wrg...@aol.com wrote: gt; Hi, I work in a Surgical Pathology frozen section lab outside the OR rooms. Normally we only perform an Hamp;E on the frozens. Now we are looking for a fast and inexpensive stain for fungus on our frozen sections. Fast is the operative word because we have to report diagnoses back to ORs in a 20 minute timeperiod. Any ideas to help? Thanks! gt; ___ gt; Histonet mailing list gt; Histonet@lists.utsouthwestern.edu gt; http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet /pre/span___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HSP70
Hi, We had decent luck with the abCam antibody ab5542 at 1:1600 with no retrieval. Good luck, Amos ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC on non-charged slides
I'm hoping Histonet can come through for me! I have an unusual case that I need to run IHC on. Antibody is Pan Keratin Cocktail from Cell Marque with recommended protocol on a Benchmark Ultra with i-view detection. Only problem is, I only have two slides and they are not charged. The tissues have been air-dried but not baked. I cannot obtain more slides. I'm wondering if there's any kind of pre-treatment I could try to try to help the tissue stay on. We have run slides like this in the past with mixed results. Most of the time, the tissue washes off. Any tips or tricks that might help out in this situation? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC on non-charged slides
After the sections are on the slides there is little (is anything) you can do to make them more resistant to the IHC protocol. My suggestions is to perform the procedure manually and instead of HIER use enzymatic retrieval that is less traumatic to the sections. René J. From: Dessoye, Michael J mjdess...@commonwealthhealth.net To: histonet@lists.utsouthwestern.edu Sent: Thursday, May 16, 2013 4:49 PM Subject: [Histonet] IHC on non-charged slides I'm hoping Histonet can come through for me! I have an unusual case that I need to run IHC on. Antibody is Pan Keratin Cocktail from Cell Marque with recommended protocol on a Benchmark Ultra with i-view detection. Only problem is, I only have two slides and they are not charged. The tissues have been air-dried but not baked. I cannot obtain more slides. I'm wondering if there's any kind of pre-treatment I could try to try to help the tissue stay on. We have run slides like this in the past with mixed results. Most of the time, the tissue washes off. Any tips or tricks that might help out in this situation? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC on non-charged slides
Michael There is a procedure where you can transfer the sections to a charges slide with a product called mount quick. You can check with newcomer supply they sell it. I know of several labs that have used it on these occasions and it works well. Sent from my iPhone On May 16, 2013, at 4:50 PM, Dessoye, Michael J mjdess...@commonwealthhealth.net wrote: I'm hoping Histonet can come through for me! I have an unusual case that I need to run IHC on. Antibody is Pan Keratin Cocktail from Cell Marque with recommended protocol on a Benchmark Ultra with i-view detection. Only problem is, I only have two slides and they are not charged. The tissues have been air-dried but not baked. I cannot obtain more slides. I'm wondering if there's any kind of pre-treatment I could try to try to help the tissue stay on. We have run slides like this in the past with mixed results. Most of the time, the tissue washes off. Any tips or tricks that might help out in this situation? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] IHC on non-charged slides
Yes, if the stain can wait get some mount quick from Newcomer. There is a good chance the tissue will fall off if you proceed with the tissue on regular slides. I would wait until I get the mount quick. Everyone else reading this should order a tube now for emergencies. You will find yourself in this position at some point and if you have the stuff on hand, it's no problem. I just used it today to transfer cells from a cytocentrifuge slide to a charged slide for FISH. It saved the patient a re-biopsy. Mark On Thu, May 16, 2013 at 2:00 PM, Debra Siena dsi...@statlab.com wrote: Michael There is a procedure where you can transfer the sections to a charges slide with a product called mount quick. You can check with newcomer supply they sell it. I know of several labs that have used it on these occasions and it works well. Sent from my iPhone On May 16, 2013, at 4:50 PM, Dessoye, Michael J mjdess...@commonwealthhealth.net wrote: I'm hoping Histonet can come through for me! I have an unusual case that I need to run IHC on. Antibody is Pan Keratin Cocktail from Cell Marque with recommended protocol on a Benchmark Ultra with i-view detection. Only problem is, I only have two slides and they are not charged. The tissues have been air-dried but not baked. I cannot obtain more slides. I'm wondering if there's any kind of pre-treatment I could try to try to help the tissue stay on. We have run slides like this in the past with mixed results. Most of the time, the tissue washes off. Any tips or tricks that might help out in this situation? Michael J. Dessoye, M.S. | Histology Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] fungal stain for frozen sections
The Sulphation Toluidine Blue stain should work a treat and very quick to do: Sulphation - Toluidine Blue Technique for Fungi Following sulphation, fungi stain metachromatically with toluidine blue. Certain hydroxyl groups are esterified by sulphuric acid to form ester sulphate groups that stain metachromatically with toluidine blue (Smith Lowrey 1986, Henwood et al 2013). Fixation: 10% buffered formalin Microtomy: 5μm paraffin sections Solutions: 1. Sulphation Reagent: For the sulphation reagent, 45 ml of glacial acetic acid is poured into a Coplin jar which has been placed into a plastic tub filled with cool tap water (not below 10°C). A 15-ml portion of concentrated sulfuric acid is slowly added with a glass pipette, being careful not to produce splashing. The solution is gently mixed with a glass rod. The Coplin jar is then sealed with petroleum jelly. The sulphation reagent is kept at room temperature and can be used for 1 week (Gosey et al 1985). 2. 3% Acetic Acid. 3. Toluidine Blue O Solution: Toluidine Blue O (CI 52040)0.01g 3% Acetic Acid 100ml 4. Metanil Yellow Counterstain: Metanil yellow (CI 13065) 0.1g Distilled Water100ml Glacial Acetic Acid0.1ml 5. Absolute Acetone. Controls: Fungi containing tissue. Procedure: 1. Cut frozen sections, air dry, defat in methanol 1 minute, rinse in water 2. Dry briefly, cover with sulphation reagent, 10 minutes. 3. Wash in running water, 5 minutes 4. Place in 3% acetic acid, 1 minute. 5. Stain in Toluidine Blue O solution, 3 minutes. 6. Rinse in 3% acetic acid, 1 minute. 7. Differentiate in absolute acetone, 5 seconds. 8. Rinse in distilled water, 5 seconds. 9. Counterstain in Metanil Yellow solution, 6 seconds. 10. Rinse in distilled water, 5 seconds. 11. Blot, dry and mount. Results: Fungi stained purple to red against a yellow background. Smith DJ, Lowrey T, (1986) A Modified Sulfation-Toluidine Blue Technique for the demonstration of fungi in tissue sections J Histotechnol. 9(1):23-24. Gosey LL, Howard RM, Witebsky FG, Ognibene FP et al (1985) Advantages of a Modified Toluidine Blue 0 Stain and Bronchoalveolar Lavage for the Diagnosis of Pneumocystis carinii Pneumonia J Clin Microbiol 22(5):803-807. Henwood, A. F., Prasad, L., Bourke, V. M. (2013). The application of heated detergent dewaxing and rehydration to techniques for the demonstration of fungi: a comparison to routine xylene-alcohol dewaxing J Histotechnol, early online DOI 10.1179/2046023613Y.25. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of wrg...@aol.com Sent: Friday, 17 May 2013 2:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fungal stain for frozen sections Hi, I work in a Surgical Pathology frozen section lab outside the OR rooms. Normally we only perform an HE on the frozens. Now we are looking for a fast and inexpensive stain for fungus on our frozen sections. Fast is the operative word because we have to report diagnoses back to ORs in a 20 minute timeperiod. Any ideas to help? Thanks! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
