Hi Bruce
Our Immuno staff have reported the uneven staining artifact with some brands of
coated slides but not others and we are staining on the Ventana platform. I
have also noticed in some cases when slides are inadequately dewaxed the
Haematoxylin will stain even though there is loss of immunostaining..
regards
Tony
Tony Reilly B.App.Sc. , M.Sc.
Chief Scientist, Anatomical Pathology
Pathology Queensland-PA Laboratory
Health Services Support Agency | Department of Health
Level 1, Building 15,Princess Alexandra Hospital
Ipswich Road,WOOLLOONGABBA Qld4102
Ph: 07 3176 2412
Mob: 0402 139411
Fax: 07 3176 2930
Email: tony_rei...@health.qld.gov.au
Web: www.health.qld.gov.au/qhcss/
Bruce Gapinski bgapin...@pathgroup.com 7/6/2013 2:57 am
Histonians!
Has anyone else had incorrect IHC staining due to overly
charged slides that are hydrophobic? My Ventana service department was out
because I kept complaining about incomplete staining. For instance, the control
at the top of the slide stained with both DAB and hematoxylin. But on the very
same slide the hematoxylin was missing from the patient tissue.
I told them how this unnerved me. If the same thing happens but
the hematoxylin stains and the DAB does not...I could be looking at a false
negative. This may have happened already as far as I know.
The solution according to Ventana is to take 4 slides from each
new box of slides (1/2 gross) and do a vortex mix on each of them one at a
time. Then you know you have valid slides to stain patient tissues! I have no
time for this. And I'm trying to believe it but it's just not scientific. How
is it that my results have been free of this phobia for over a year? Why
didn't I get this (EVER) with Dako instrumentation? The answer, I was told, was
that the humidity was a problem. If I were to take this to it's ridiculous
conclusion, I would need to inform oncologists not to operate during these
incorrectly humidified days. It IS summer so I'm not sure how much humidity is
required.
1. What brand of slides do you use?
2. Have you had the slide phobia so that it compromised the results?
(Slide-phobia is for retired Histologists, no?)
3. (I think I know the answer but I just have to ask) Do you validate
slides?
Bruce Gapinsk HT (ASCP)
Chief Histologist
Marin Medical Laboratories
PathGroup SF
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