Re: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde
Thanks to everyone for your comments. I may not have been clear in my question - our researchers don't wish to decalcify these formalin-fixed bones yet, but rather to store them for more than a couple of weeks, in case they need to carry out MicroCT followed by histology later. I'm aware that the formalin or paraformaldehyde will degrade over time, but I just wondered if anyone has a protocol for storage without decalcification? I guess transfer to 70% ethanol is an option but this is also not ideal for longterm storage, and would need to be removed before decal in EDTA. All the best, Orla On 6 December 2013 16:12, Wineman, Terra terra.wine...@novusint.com wrote: I would suggest a different protocol if the tissue will not be processed for a while. I would say a week in 10%NBF and then transfer the bones to an EDTA decal solution. The bones will decal slowly without the affects of the formic acid. I am in research and this is what we do with our bones. Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wine...@novusint.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pru...@ihctech.net Sent: Thursday, December 05, 2013 2:50 PM To: gu.l...@gmx.at; 'Orla M Gallagher' Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde i would think u are correct in advising formic acid decal and then processing into paraffin for the best protection of the trap enzyme, immunoreactivity, etc. A couple of weeks in formalin should be fine. Paraformaldehyde show be the same as formalin. I do know a way to restore the enzyme activity for TRAP that may have been lost so if u need that let me know. - Original Message - Subject: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde From: Gudrun Lang gu.l...@gmx.at Date: 12/5/13 11:42 am To: 'Orla M Gallagher' o.m.gallag...@sheffield.ac.uk Cc: histonet@lists.utsouthwestern.edu Paraformaldehyd is formaldehyd in solid form. Formalin is the aequous solution of formaldehyd. So the main characteristics are the same. Gudrun Lang -Urspruuml;ngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Orla M Gallagher Gesendet: Donnerstag, 05. Dezember 2013 19:31 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde Dear Histonetters, What is your opinion on storing bone samples long-term (more than a couple of weeks) in 10% formalin? As I was taught, best practice has always been to fix only as long as necessary, depending on the size of the sample, then decalcify and process to wax, and I always stress this to everyone I advise. However, research colleagues sometimes wish to do histology on bone samples that have been stored for months ..or even years! As the formalin pH becomes more acidic, there is formalin pigment and the immunoreactivity and TRAP enzyme activity is diminished or destroyed during long fixation, is there any way of minimising this e.g. has anyone tried regularly replacing the old formalin with fresh buffered formalin, or storing formalin-fixed bones in any other medium? I'm also interested in how best to fix in 4% paraformaldehyde and whether the problems are the same with long-term storage. Thanks for your comments. All the best, Orla -- ** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallag...@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. *Times Higher Education University of the Year* Data protection and confidentiality: The information contained in this message or any appended documents may be privileged and confidential and is intended for the exclusive use of the addressee(s). If you are not the addressee, any disclosure, reproduction, distributions, other dissemination or use of this message is strictly prohibited and may be unlawful. If you receive this correspondence in error please contact the sender immediately and permanently delete/destroy what you have received. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
Re: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde
hi I would recommend storage for long term in 70% ethanol. To prevent drying out we used glycerin in the ethanol, about 20% of the volume. Barry On Mon, Dec 9, 2013 at 7:44 AM, Orla M Gallagher o.m.gallag...@sheffield.ac.uk wrote: Thanks to everyone for your comments. I may not have been clear in my question - our researchers don't wish to decalcify these formalin-fixed bones yet, but rather to store them for more than a couple of weeks, in case they need to carry out MicroCT followed by histology later. I'm aware that the formalin or paraformaldehyde will degrade over time, but I just wondered if anyone has a protocol for storage without decalcification? I guess transfer to 70% ethanol is an option but this is also not ideal for longterm storage, and would need to be removed before decal in EDTA. All the best, Orla On 6 December 2013 16:12, Wineman, Terra terra.wine...@novusint.com wrote: I would suggest a different protocol if the tissue will not be processed for a while. I would say a week in 10%NBF and then transfer the bones to an EDTA decal solution. The bones will decal slowly without the affects of the formic acid. I am in research and this is what we do with our bones. Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wine...@novusint.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pru...@ihctech.net Sent: Thursday, December 05, 2013 2:50 PM To: gu.l...@gmx.at; 'Orla M Gallagher' Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde i would think u are correct in advising formic acid decal and then processing into paraffin for the best protection of the trap enzyme, immunoreactivity, etc. A couple of weeks in formalin should be fine. Paraformaldehyde show be the same as formalin. I do know a way to restore the enzyme activity for TRAP that may have been lost so if u need that let me know. - Original Message - Subject: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde From: Gudrun Lang gu.l...@gmx.at Date: 12/5/13 11:42 am To: 'Orla M Gallagher' o.m.gallag...@sheffield.ac.uk Cc: histonet@lists.utsouthwestern.edu Paraformaldehyd is formaldehyd in solid form. Formalin is the aequous solution of formaldehyd. So the main characteristics are the same. Gudrun Lang -Urspruuml;ngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Orla M Gallagher Gesendet: Donnerstag, 05. Dezember 2013 19:31 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde Dear Histonetters, What is your opinion on storing bone samples long-term (more than a couple of weeks) in 10% formalin? As I was taught, best practice has always been to fix only as long as necessary, depending on the size of the sample, then decalcify and process to wax, and I always stress this to everyone I advise. However, research colleagues sometimes wish to do histology on bone samples that have been stored for months ..or even years! As the formalin pH becomes more acidic, there is formalin pigment and the immunoreactivity and TRAP enzyme activity is diminished or destroyed during long fixation, is there any way of minimising this e.g. has anyone tried regularly replacing the old formalin with fresh buffered formalin, or storing formalin-fixed bones in any other medium? I'm also interested in how best to fix in 4% paraformaldehyde and whether the problems are the same with long-term storage. Thanks for your comments. All the best, Orla -- ** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallag...@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. *Times Higher Education University of the Year* Data protection and confidentiality: The information contained in this message or any appended documents may be privileged and confidential and is intended for the exclusive use of the addressee(s). If you are not the addressee, any disclosure, reproduction, distributions, other dissemination or use of this message is strictly prohibited and may be unlawful. If you receive this correspondence in error please contact the sender
RE: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde
Just curious-what do people consider the time frame for long term ? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barry Rittman Sent: Monday, December 09, 2013 7:50 AM To: Orla M Gallagher Cc: histonet@lists.utsouthwestern.edu; Wineman, Terra Subject: Re: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde hi I would recommend storage for long term in 70% ethanol. To prevent drying out we used glycerin in the ethanol, about 20% of the volume. Barry On Mon, Dec 9, 2013 at 7:44 AM, Orla M Gallagher o.m.gallag...@sheffield.ac.uk wrote: Thanks to everyone for your comments. I may not have been clear in my question - our researchers don't wish to decalcify these formalin-fixed bones yet, but rather to store them for more than a couple of weeks, in case they need to carry out MicroCT followed by histology later. I'm aware that the formalin or paraformaldehyde will degrade over time, but I just wondered if anyone has a protocol for storage without decalcification? I guess transfer to 70% ethanol is an option but this is also not ideal for longterm storage, and would need to be removed before decal in EDTA. All the best, Orla On 6 December 2013 16:12, Wineman, Terra terra.wine...@novusint.com wrote: I would suggest a different protocol if the tissue will not be processed for a while. I would say a week in 10%NBF and then transfer the bones to an EDTA decal solution. The bones will decal slowly without the affects of the formic acid. I am in research and this is what we do with our bones. Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wine...@novusint.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pru...@ihctech.net Sent: Thursday, December 05, 2013 2:50 PM To: gu.l...@gmx.at; 'Orla M Gallagher' Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde i would think u are correct in advising formic acid decal and then processing into paraffin for the best protection of the trap enzyme, immunoreactivity, etc. A couple of weeks in formalin should be fine. Paraformaldehyde show be the same as formalin. I do know a way to restore the enzyme activity for TRAP that may have been lost so if u need that let me know. - Original Message - Subject: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde From: Gudrun Lang gu.l...@gmx.at Date: 12/5/13 11:42 am To: 'Orla M Gallagher' o.m.gallag...@sheffield.ac.uk Cc: histonet@lists.utsouthwestern.edu Paraformaldehyd is formaldehyd in solid form. Formalin is the aequous solution of formaldehyd. So the main characteristics are the same. Gudrun Lang -Urspruuml;ngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Orla M Gallagher Gesendet: Donnerstag, 05. Dezember 2013 19:31 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde Dear Histonetters, What is your opinion on storing bone samples long-term (more than a couple of weeks) in 10% formalin? As I was taught, best practice has always been to fix only as long as necessary, depending on the size of the sample, then decalcify and process to wax, and I always stress this to everyone I advise. However, research colleagues sometimes wish to do histology on bone samples that have been stored for months ..or even years! As the formalin pH becomes more acidic, there is formalin pigment and the immunoreactivity and TRAP enzyme activity is diminished or destroyed during long fixation, is there any way of minimising this e.g. has anyone tried regularly replacing the old formalin with fresh buffered formalin, or storing formalin-fixed bones in any other medium? I'm also interested in how best to fix in 4% paraformaldehyde and whether the problems are the same with long-term storage. Thanks for your comments. All the best, Orla -- ** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallag...@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. *Times Higher Education University of the
Re: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde
Dear Orla, Post fixation, we have stored our bone specimens in 1x PBS while having them sent out for MicroCT analysis. We have also stored them in 1x PBS at 4°C post fixation when necessary until further processing without having adverse affects on our staining. However, we chose conventional staining over IHC for our results. Best regards, ~Sean McBride Scientific Specialist Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 412-268-8275 (o) 412-915-1683 (m) 412-268-8275 (fax) smcbr...@andrew.cmu.edu On Dec 9, 2013, at 8:44 AM, Orla M Gallagher wrote: Thanks to everyone for your comments. I may not have been clear in my question - our researchers don't wish to decalcify these formalin-fixed bones yet, but rather to store them for more than a couple of weeks, in case they need to carry out MicroCT followed by histology later. I'm aware that the formalin or paraformaldehyde will degrade over time, but I just wondered if anyone has a protocol for storage without decalcification? I guess transfer to 70% ethanol is an option but this is also not ideal for longterm storage, and would need to be removed before decal in EDTA. All the best, Orla On 6 December 2013 16:12, Wineman, Terra terra.wine...@novusint.com wrote: I would suggest a different protocol if the tissue will not be processed for a while. I would say a week in 10%NBF and then transfer the bones to an EDTA decal solution. The bones will decal slowly without the affects of the formic acid. I am in research and this is what we do with our bones. Terra Wineman, HTL (ASCP)CM Research Biologist 636-926-7476 phone terra.wine...@novusint.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pru...@ihctech.net Sent: Thursday, December 05, 2013 2:50 PM To: gu.l...@gmx.at; 'Orla M Gallagher' Cc: histonet@lists.utsouthwestern.edu Subject: RE: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde i would think u are correct in advising formic acid decal and then processing into paraffin for the best protection of the trap enzyme, immunoreactivity, etc. A couple of weeks in formalin should be fine. Paraformaldehyde show be the same as formalin. I do know a way to restore the enzyme activity for TRAP that may have been lost so if u need that let me know. - Original Message - Subject: AW: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde From: Gudrun Lang gu.l...@gmx.at Date: 12/5/13 11:42 am To: 'Orla M Gallagher' o.m.gallag...@sheffield.ac.uk Cc: histonet@lists.utsouthwestern.edu Paraformaldehyd is formaldehyd in solid form. Formalin is the aequous solution of formaldehyd. So the main characteristics are the same. Gudrun Lang -Urspruuml;ngliche Nachricht- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Orla M Gallagher Gesendet: Donnerstag, 05. Dezember 2013 19:31 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Bone samples long-term storage in 10% formalin or 4% paraformaldehyde Dear Histonetters, What is your opinion on storing bone samples long-term (more than a couple of weeks) in 10% formalin? As I was taught, best practice has always been to fix only as long as necessary, depending on the size of the sample, then decalcify and process to wax, and I always stress this to everyone I advise. However, research colleagues sometimes wish to do histology on bone samples that have been stored for months ..or even years! As the formalin pH becomes more acidic, there is formalin pigment and the immunoreactivity and TRAP enzyme activity is diminished or destroyed during long fixation, is there any way of minimising this e.g. has anyone tried regularly replacing the old formalin with fresh buffered formalin, or storing formalin-fixed bones in any other medium? I'm also interested in how best to fix in 4% paraformaldehyde and whether the problems are the same with long-term storage. Thanks for your comments. All the best, Orla -- ** Ms. Orla Gallagher Bone Analysis Laboratory Mellanby Centre for Bone Research Department of Human Metabolism D Floor Medical School University of Sheffield Beech Hill Road Sheffield S10 2RX UK Website: http://mellanbycentre.dept.shef.ac.uk Tel: 0044114-2713337 (office) 0044114-2713174 (lab) E-Mail: o.m.gallag...@sheffield.ac.uk *STOP*: Do you really need to print this e-mail? *BE GREEN:* Keep it on the screen. *Times Higher Education University of the Year* Data protection and confidentiality: The information contained in this message or any appended documents may be privileged and confidential and is intended for
[Histonet] CD4 and CD8 mouse tumor IHC, thanks..
Thank you to those that sent me suggestion on my CD4 problem. I have recently discovered the problem. The tumors are mouse tumors but they are injected in-vivo with a test antibody that is Rat anti-mouse, specifically Isotype (Rat IgG2a). Hence this is why my secondary Rabbit anti-rat stained so much.. Next I will try to block the Rat in-vivo injected antibody with a goat anti-Rat secondary, as a block prior to staining with CD4. Or I could precomplex the CD4 with the Rabbit Anti-Rat and bind excess Anti-Rat with Rat Serum. Any thoughts or suggestion on this as always would be welcomed... Thanks again.. Jamie Abbvie Bioresearch Center Pharmacology 100 Research Dr. Worcester, Ma 01605 OFFICE+1 508-688-3134 FAX +1 508-793-4895 EMAIL jamie.erick...@abbvie.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] CD4 and CD8 mouse tumor IHC, thanks..
Another solution, and one we did almost exclusively for murine T and B cell IHC was use biotinylated Rat anti mouse CD4 and CD8. The isotype can be purchased biotinylated from BD Bioscience/Invitrogen or you can buy Jacksons Rat IgG-biotin. This totally eliminates a secondary antibody hence anything rat will not be detected. A bonus is staining is much faster too. We routinely did Streptavidin/biotin block from Vector since Streptavidin has an affinity for integrins in epithelial cells.Our normal serum block with biotinylated rat antimouse primaries is 10% goat or donkey serum + 2.5% mouse. The block is also the diluent for the primary antibody and isotype/IgG negative control. Rabbit serum and antibodies were avoided since the bunny is sticky, and can cause more background. My immunologist frowned on anything using a rabbit host unless we couldn't avoid it. Good luck Gayle Callis HTL/HT/MT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Erickson, Jamie E Sent: Monday, December 09, 2013 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD4 and CD8 mouse tumor IHC, thanks.. Thank you to those that sent me suggestion on my CD4 problem. I have recently discovered the problem. The tumors are mouse tumors but they are injected in-vivo with a test antibody that is Rat anti-mouse, specifically Isotype (Rat IgG2a). Hence this is why my secondary Rabbit anti-rat stained so much.. Next I will try to block the Rat in-vivo injected antibody with a goat anti-Rat secondary, as a block prior to staining with CD4. Or I could precomplex the CD4 with the Rabbit Anti-Rat and bind excess Anti-Rat with Rat Serum. Any thoughts or suggestion on this as always would be welcomed... Thanks again.. Jamie Abbvie Bioresearch Center Pharmacology 100 Research Dr. Worcester, Ma 01605 OFFICE+1 508-688-3134 FAX +1 508-793-4895 EMAIL jamie.erick...@abbvie.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Discontinuing Negative Reagent Controls for IHC
I am putting together a list of facilities that have discontinued the use of negative reagent controls for IHC. After the CAP revised the requirement related to negative controls when polymer-based detection systems are used, we decided to investigate whether discontinuing the negative controls would be possible for our lab. It would be helpful to know what labs have done this successfully. If the labs that have discontinued the use of negatives could just respond in an email, I would appreciate it. Also, if anyone has any thoughts pertaining to this change, they're certainly welcome. Thank you, Roger Maywood, IL ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Discontinuing Negative Reagent Controls for IHC
Hartford Hospital, Hartford, CT Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.org From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Roger Heyna [rhe...@lumc.edu] Sent: Monday, December 09, 2013 3:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Discontinuing Negative Reagent Controls for IHC I am putting together a list of facilities that have discontinued the use of negative reagent controls for IHC. After the CAP revised the requirement related to negative controls when polymer-based detection systems are used, we decided to investigate whether discontinuing the negative controls would be possible for our lab. It would be helpful to know what labs have done this successfully. If the labs that have discontinued the use of negatives could just respond in an email, I would appreciate it. Also, if anyone has any thoughts pertaining to this change, they're certainly welcome. Thank you, Roger Maywood, IL This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ASCP Certified Pathologist Assistant Job in Nebraska (Perm or Temp to Perm)
Hello, I have a Full Time/Permanent Pathologists Assistant position available in the Omaha, NE area. This position requires ASCP certification as a Pathologist Assistant. I am ideally looking for someone for a long term/permanent position however, a temp to perm option may be available. Relocation assistance available if permanent position is accepted. Please send resume if interested. Thank you, To view a complete list of Allied Search Partners current openings go to: http://www.alliedsearchpartners.com/careers.php -- Melissa Phelan President, Laboratory Staffing Allied Search Partners www.linkedin.com/in/melissaphelan/ http://www.linkedin.com/in/melissaphelan/ http://www.alliedsearchpartners.com http://www.alliedsearchpartners.com/ T: 888.388.7571 ext. 102 F: 888.388.7572 *If you wish to no longer receive emails from Allied Search Partners please respond to this email message with remove. This email including its attachments is intended only for the confidential use of the individual to whom it is addressed. If you are not the intended recipient, any use, dissemination, distribution or copying of this message or its attachments is prohibited. If you have received this message in error, please notify us immediately, and delete this message and its attachments permanently from your system. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
