[Histonet] IHC Lead Tech Position - Dallas
LEAD IMMUNOHISTOCHEMISTRY TECHNICIAN ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Lead Immunohistochemistry Technician for its' Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. The ideal candidate will have a minimum of 5 years Histology experience with paraffin microtomy with a variety of different tissue types, prefer at least 3 years immunohistochemistry, immunofluorescence or in situ hybridization and frozen section experience. Technical Lead/Coordinator experience is required. Working knowledge of IHC theory required, hands on IHC performance desired. If using an automated system we'll easily train you on our manual system. HT (ASCP) or QIHC desired. The hours for the position are 7:00 p.m. to 3:30 a.m. Monday through Friday. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EOE Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to accessibil...@propath.com. Pat Patterson, HTL(ASCP) Supervisor, Immunohistochemistry ProPath - The Leader in Pathology Services 1355 River Bend Drive Dallas, TX 75247 pat.patter...@propath.commailto:pat.patter...@propath.com 214-237-1700 x 2027 214-237-1730 fax To learn more about ProPath, please visit http://www.ProPath.com This electronic message is intended to be for the use only of the named recipient and may contain information that is confidential or privileged. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this message in error or are not the named recipient, please notify us immediately by contacting the sender at the electronic mail address noted above, and delete and destroy all copies of this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Charges for recuts on legal cases
Can anyone share with me what they charge for doing recuts on cases that have been pulled for legal purposes? I am trying to see what others charge before we assign a charge. I think most people are probably charging by the slide but please let me know if you charge differently. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Staining on Alcohol Fixed Smears
Since there is no exposure to formalin, we do not do antigen retrieval on alcohol fixed specimens. We use the Ventana Benchmark XT and copy the protocol to a new number and use the Wet option rather than paraffin. No retrieval needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Saturday, December 14, 2013 6:30 PM To: histonet@lists.utsouthwestern.edu; levi.fr...@gmail.com Subject: [Histonet] Re: Staining on Alcohol Fixed Smears Levi, I've worked up most of the more common antibodies on alcohol fixed smears. There are two things to remember here: 1) your specimens are alcohol fixed, meaning antigen retrieval needs are different, and 2) they are smears, meaning the cells weren't 'cut open' during microtomy, which impeeds some nuclear staining. Those are two different issues to consider. Some rules of thumb: 1) most cell membrane antigens will stain nicely with minimal changes to the protocols you use for FFPE specimens 2) most cytokeratin antigens stain nicely with little change to your FFPE protocols (if you need to change, start by cutting your antigen retrieval time in half) 3) most nuclear antigens require significant changes from FFPE protocols, and some are essentially impossible to stain using standard antibodies. When I return to work on Monday, I will forward you my protocol 'adjustments' for the antibodies you requested. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech On 12/14/2013 12:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Message: 4 Date: Sat, 14 Dec 2013 18:25:08 +0200 From: Levi Friedlevi.fr...@gmail.com Subject: [Histonet] Staining on Alcohol Fixed Smears. To:histonet@lists.utsouthwestern.edu Message-ID: CA+puqxTXL85pEwvR=sqPQA36tMJPm=sx2kv3gyebrbu0he3...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi Everyone. I am looking to stain alcohol fixed smears with a group of antibodies. The antibodies of particular interest are p63, p53 and ki67. Along with these antibodies I am looking for positive nuclear and cytoplastic staining antibodies. If anyone has any experience in staining alcohol fixed slides your information is very appreciated. All the best. -- Sincerely, - Levi Fried ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Charges for recuts on legal cases
I charge a $30.00 fee for investigating the case and issuing a report. I offer 1 HE and 2 unstained sections on plus slides and charge an additional $15.00 per slide. They furnish the shipping/FedEx. W WANDA G. SMITH, HTL(ASCP)HT Pathology Supervisor TRIDENT MEDICAL CENTER 9330 Medical Plaza Drive Charleston, SC 29406 843-847-4586 843-847-4296 fax This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Monday, December 16, 2013 11:17 AM To: histonet@lists.utsouthwestern.edu Cc: Bantner, Diane Subject: [Histonet] Charges for recuts on legal cases Can anyone share with me what they charge for doing recuts on cases that have been pulled for legal purposes? I am trying to see what others charge before we assign a charge. I think most people are probably charging by the slide but please let me know if you charge differently. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Staining on Alcohol Fixed Smears
I agree that with no formalin exposure, there is no crosslinking that needs to be broken, but a then, do nothing approach to antigen retrieval oversimplifies the issue. Alcohol fixation makes changes in the protiens/antibodies also, although different changes than formalin does, and these changes need to be accounted for. In my research on alcohol fixed smears, I found that most antibodies perform best with some antigen retrieval, often gentler than formalin tissues require. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech -- Message: 4 Date: Mon, 16 Dec 2013 11:13:59 -0500 From: Tom McNemartmcne...@lmhealth.org Subject: RE: [Histonet] Re: Staining on Alcohol Fixed Smears To: 'Beth Cox'bethc...@gmail.com, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu,levi.fr...@gmail.com levi.fr...@gmail.com Message-ID: E9A90E28259D2F4E84308C5E8EA8F7B4016951E94252@lmhs-exchange Content-Type: text/plain; charset=us-ascii Since there is no exposure to formalin, we do not do antigen retrieval on alcohol fixed specimens. We use the Ventana Benchmark XT and copy the protocol to a new number and use the Wet option rather than paraffin. No retrieval needed. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcne...@lmhealth.org www.LMHealth.org -Original Message- From:histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Cox Sent: Saturday, December 14, 2013 6:30 PM To:histonet@lists.utsouthwestern.edu;levi.fr...@gmail.com Subject: [Histonet] Re: Staining on Alcohol Fixed Smears Levi, I've worked up most of the more common antibodies on alcohol fixed smears. There are two things to remember here: 1) your specimens are alcohol fixed, meaning antigen retrieval needs are different, and 2) they are smears, meaning the cells weren't 'cut open' during microtomy, which impeeds some nuclear staining. Those are two different issues to consider. Some rules of thumb: 1) most cell membrane antigens will stain nicely with minimal changes to the protocols you use for FFPE specimens 2) most cytokeratin antigens stain nicely with little change to your FFPE protocols (if you need to change, start by cutting your antigen retrieval time in half) 3) most nuclear antigens require significant changes from FFPE protocols, and some are essentially impossible to stain using standard antibodies. When I return to work on Monday, I will forward you my protocol 'adjustments' for the antibodies you requested. Beth Cox, HTL/SCT(ASCP)QIHC Vagabond Histotech On 12/14/2013 12:00 PM,histonet-requ...@lists.utsouthwestern.edu wrote: Message: 4 Date: Sat, 14 Dec 2013 18:25:08 +0200 From: Levi Friedlevi.fr...@gmail.com Subject: [Histonet] Staining on Alcohol Fixed Smears. To:histonet@lists.utsouthwestern.edu Message-ID: CA+puqxTXL85pEwvR=sqPQA36tMJPm=sx2kv3gyebrbu0he3...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hi Everyone. I am looking to stain alcohol fixed smears with a group of antibodies. The antibodies of particular interest are p63, p53 and ki67. Along with these antibodies I am looking for positive nuclear and cytoplastic staining antibodies. If anyone has any experience in staining alcohol fixed slides your information is very appreciated. All the best. -- Sincerely, - Levi Fried ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Histonet Digest, Vol 121, Issue 21
re: per slide cost for IHC I realize there are differences in reagents, including antibody prices. Still, I sometimes am asked to provide the average cost to produce a single IHC slide. If anyone has an available estimate, especially broken down (glass cost, antibody cost, labor cost...), I would greatly appreciate it. Thank you. --- Scott Ely, MD MPH Associate Director, Hematopathology Fellowship Program Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital Room: Starr 715 525 E. 68th Street New York, NY 10065 PH: 212-746-2442 FAX: 212-746-2009 http://www.cornellphysicians.com/scottely/ Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. --- From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Monday, December 16, 2013 1:07 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 121, Issue 21 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. rabbit cea antibody for mouse (Mehmet Fatih BOZKURT) 2. IHC Lead Tech Position - Dallas (Pat Patterson) 3. Freezer for tissue storage (Wester, Martha) 4. RE: Re: Staining on Alcohol Fixed Smears (Tom McNemar) 5. Charges for recuts on legal cases (Vickroy, Jim) -- Message: 1 Date: Mon, 16 Dec 2013 09:02:54 +0200 From: Mehmet Fatih BOZKURT fbozk...@gmail.com Subject: [Histonet] rabbit cea antibody for mouse To: ih...@googlegroups.com, Histonet@lists.utsouthwestern.edu Message-ID: CA+pSMGTJu1mUUKnNydd45UD76AsXv=_oa_t2nma+f-2jlq4...@mail.gmail.com Content-Type: text/plain; charset=ISO-8859-1 Hello, I'm looking for rabbit CEA antibody for using on mouse tissue. Anyone can help me? Thanks. -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-16173/16237 -- Message: 2 Date: Mon, 16 Dec 2013 14:41:51 + From: Pat Patterson pat.patter...@propath.com Subject: [Histonet] IHC Lead Tech Position - Dallas To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 6dcb8b92d0138244b56ce8eace0d458d1675b...@mail.propathlab.com Content-Type: text/plain; charset=us-ascii LEAD IMMUNOHISTOCHEMISTRY TECHNICIAN ProPath, a progressive, CAP accredited, high-volume pathology practice in Dallas, Texas is seeking an Lead Immunohistochemistry Technician for its' Immunohistochemistry Lab. Responsibilities include slide preparation (paraffin and frozen sections), IHC staining using our unique manual system, antibody titer preparation, equipment maintenance, supply/reagent inventory maintenance, and QC/QA recording. The ideal candidate will have a minimum of 5 years Histology experience with paraffin microtomy with a variety of different tissue types, prefer at least 3 years immunohistochemistry, immunofluorescence or in situ hybridization and frozen section experience. Technical Lead/Coordinator experience is required. Working knowledge of IHC theory required, hands on IHC performance desired. If using an automated system we'll easily train you on our manual system. HT (ASCP) or QIHC desired. The hours for the position are 7:00 p.m. to 3:30 a.m. Monday through Friday. ProPath utilizes leading technology and is a quality oriented pathology laboratory. Benefits include medical, dental, Short and Long Term Disability insurance, a matched 401K plan and more! Don't Follow the Leader! Join the Leader! To apply, please visit www.propath.com EOE Accessibility Accommodations If you require an accommodation to navigate or apply to our careers site, please send your request to
[Histonet] Perfusion of mouse bone femur
Does anybody have a Protocol for perfusion of mouse bone femur. It looks like my protocol does not work. 1.fix in 4% PFA in PBS for 24 hrs in refrigerator 2. wash in PBS 3x 3. transfer in 14 %EDTA for 5 days. 4. Wash in water for maximum of 2 hrs in running water 6. perfusion with 15% sucrose overnight 7.perfusion with 30% overnight. 8. Transfer in OCT. 8. Cut in 10 um and transfer section on APES slide Results: When cutting section, the tissue does not adhere to the slides properly. Can you please help us or any comment to correct this protocol. Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Charges for recuts on legal cases
Here in Connecticut we are not allowed to charge for the preparation of pathology slides for legal purposes. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.org From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Vickroy, Jim [vickroy@mhsil.com] Sent: Monday, December 16, 2013 11:16 AM To: histonet@lists.utsouthwestern.edu Cc: Bantner, Diane Subject: [Histonet] Charges for recuts on legal cases Can anyone share with me what they charge for doing recuts on cases that have been pulled for legal purposes? I am trying to see what others charge before we assign a charge. I think most people are probably charging by the slide but please let me know if you charge differently. Thanks James Vickroy BS, HT(ASCP) Surgical and Autopsy Pathology Technical Supervisor Memorial Medical Center 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] variable staining of alcian blue
Hello, I am staining paraffin sections (10 microns) with Alcian blue and Nuclear Fast Red as standard protocol as below. The problem is that I see some sections stained with alicain blue show some variability (hope i am able to attach image); alcian blue stain are washed out in some sections even in the same slide. Those sections show pink color in the cartilage. Clearing dehydrate 1% Alcain blue (PH2.5) 30 mins dip in tap water running water 2mins dip deionized water 0.1% Nuclear Fast red 5 mins dip deionized water 30 sec X 2 95% ethanol 1min 100% ethanol 1 mins X 2 clearing Is this something to do with filtering alcian blue or nuclear fast red? I filtered alcian blue using needles to eliminate a big undisolved particle, but I did not filter the nuclear fast red since it seems all dissolved well. also I rarely had the problem last time i used the same staining. I know i need to filter with filter paper but when i tried, even with non-fine filter paper, it absorbed too much dye and was not successful. I tried to wash nuclear fast red with running tap water 1 min and dip in deionized water intead of twice wash of deionized water as described above. However, variable staining of alcian blue still present. Does anybody have same problem before? I appreciate any suggestion to solve this. Thanks. Rui ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
