[Histonet] RE: TAT for Transplant Biopsies
We accept biopsies until 10:30 a.m. Monday through Friday. If it is an extreme emergency, we will accept a later cut off time and someone stays to take care of it. Yes, we will process transplant biopsies on weekends and holidays. We do not process the biopsies at night. Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org archildrens.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cartun, Richard Sent: Thursday, January 09, 2014 6:47 PM To: Histonet Subject: [Histonet] TAT for Transplant Biopsies For those of you seeing biopsies of transplant kidney, liver, and heart, what is your cut-off time for accepting a specimen for same-day processing? Do any of you handle specimens at night, weekends, or holidays? Thank you! Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ** The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Frozen sections
Good morning, I know all histotechs help cut and stain frozen sections. Is there any organization out there that has the histotechnician gross the frozen section tissue and place on the frozen section chuck to cut without the Pathologist in the room. If anyone does this please tell me why? And if your organization doesn't do this please tell me why. I am having a debate with our Pathologists over this question. Thank You, LeAnn Murphy Aultman Hospital Canton, Ohio ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] ROS-1
You might try contacting Cell Signaling Technology in Danvers, MA. They carry a rabbit mAb to ROS1 and I believe they have control slides that can be used for validation purposes. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.org From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Mark Tarango [marktara...@gmail.com] Sent: Friday, January 03, 2014 2:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] ROS-1 Hi All, Does anyone have a case of lung tumor positive for ROS1 by either IHC or FISH? We are working on validating this by FISH and haven't seen a positive case. We are going to order a positive cell line but we'll be running this on tissue, so if anyone could spare a few slides or a block that would very helpful. thank you! Mark Tarango ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] controls
Is a candid control one that's taken when the fungus doesn't know you're sampling it? Sincerely? Jay A Lundgren, M.S., (HTL) ASCP On Fri, Jan 10, 2014 at 11:58 AM, Webb, Dorothy L dorothy.l.w...@healthpartners.com wrote: Does anyone out in histoland have a Candid control for fungus that you could spare? We are very much in need and would appreciate the help and see what we could possibly trade for. Much thanks and have a great week-end all! Dorothy Webb, HT (ASCP) Regions Histology Technical Specialist 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processing:
I have one tech telling me that when the entire processor is changed the tissue is too dry. We run a lot of fatty tissues, breast, etc on this processor. (Our biopsies are run on a separate processor). Is this correct, or should we only rotate reagents? No other techs complain. I have a hard time believing this, my experience is the opposite. Any input is appreciated. Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Processing:
This depends on so many different factors, however, I prefer a frequent rotation over a complete change. Do what is best for your tissue! Sent from my iPhone On Jan 13, 2014, at 9:46 AM, Jb craiga...@gmail.com wrote: I have one tech telling me that when the entire processor is changed the tissue is too dry. We run a lot of fatty tissues, breast, etc on this processor. (Our biopsies are run on a separate processor). Is this correct, or should we only rotate reagents? No other techs complain. I have a hard time believing this, my experience is the opposite. Any input is appreciated. Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Processing:
This gets me back to another recent topic, soaking the blocks. I've seen this a little in the past, just soak them on an ice block,tray for a couple minutes and you'll be fine. To me, another indicator would be that if you're getting dry tissue when changed but not later could there be some kind of variation in results??? How often do you change the processors, all the tissue A complete change every other day would probably get you consistent results, at least, even if they are a little dry. Just my experience. Curt -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jb Sent: Monday, January 13, 2014 8:46 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Processing: I have one tech telling me that when the entire processor is changed the tissue is too dry. We run a lot of fatty tissues, breast, etc on this processor. (Our biopsies are run on a separate processor). Is this correct, or should we only rotate reagents? No other techs complain. I have a hard time believing this, my experience is the opposite. Any input is appreciated. Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] myeloperoxidase on FFPE or frozen sections
Dear Histonetters, I wonder if anyone has done myeloperoxidase stain on FFPE or frozen lung sections? I would appreciate any help. Regards, Mesru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] per diem tech in Boston area
Fellow techs, I have a per diem histo tech position in the Boston area. We are a small uropath lab and the duties will include embedding, sectioning and IHC's. thanks, Ron Martin ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] microwave processing
Hi! Can someone recommend literature about microwave processing. I'm interested in the physical principles behind the process. And I want to get answers to the questions: why is this microwave-assisted infiltration faster? What happens to proteins /antigens under microwave radiation? Is there a difference between conventional or microwave processing in relation to antigen preservation after usual formalinfixation. Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Frozen sections
Our pathologists prefer to perform all aspects of frozen section preparation. We will stain for them, but they would rather gross and section themselves. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leann M. Murphy Sent: Monday, January 13, 2014 10:35 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen sections Good morning, I know all histotechs help cut and stain frozen sections. Is there any organization out there that has the histotechnician gross the frozen section tissue and place on the frozen section chuck to cut without the Pathologist in the room. If anyone does this please tell me why? And if your organization doesn't do this please tell me why. I am having a debate with our Pathologists over this question. Thank You, LeAnn Murphy Aultman Hospital Canton, Ohio ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microwave processing
Perhaps you can get some literature from one of the vendors that sell that technology. Milestone Medical Sakura Walter Benton HT(ASCP)QIHC Histology Supervisor Chesapeake Urology Associates 806 Landmark Drive, Suite 127 Glen Burnie, MD 21061 443-471-5850 (Direct) 410-768-5961 (Lab) 410-768-5965 (Fax) ChesapeakeUrology.com Voted a Best Place to Work by Baltimore and Modern Healthcare Magazines. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang [gu.l...@gmx.at] Sent: Monday, January 13, 2014 1:56 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave processing Hi! Can someone recommend literature about microwave processing. I'm interested in the physical principles behind the process. And I want to get answers to the questions: why is this microwave-assisted infiltration faster? What happens to proteins /antigens under microwave radiation? Is there a difference between conventional or microwave processing in relation to antigen preservation after usual formalinfixation. Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The information contained in this electronic message is intended solely for the personal and confidential use of the designated recipient(s) named above and may contain information that is protected from disclosure under applicable law. If you are not the intended recipient, or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this transmission is strictly prohibited. If you have received this transmission in error, please notify the transmitting person/department immediately by email or telephone (410) 581-5881 and delete the message without making a copy. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Techs grossing FS tissue
This is the only way that a non-pathologist can gross tissue, whether for frozen section or otherwise. They must meet CLIA standards for high complexity testing and furthermore, CAP says that the exact nature of the tissue grossed must be spelled out, along with nature of the pathologists' supervision (direct, indirect), and have at least annual competencies on grossing on file. That's the basic rules. State licensing may possibly add, but never subtract to this. However, there is nothing that says a tech can't plop a small singular piece of tissue onto a chuck to freeze and get things started. Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Message: 3 Date: Mon, 13 Jan 2014 15:34:48 + From: Leann M. Murphy lmurp...@aultman.com Good morning, I know all histotechs help cut and stain frozen sections. Is there any organization out there that has the histotechnician gross the frozen section tissue and place on the frozen section chuck to cut without the Pathologist in the room. If anyone does this please tell me why? And if your organization doesn't do this please tell me why. I am having a debate with our Pathologists over this question. Thank You, LeAnn Murphy Aultman Hospital Canton, Ohio - CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] microwave processing
Gudrun, A good overview is here: http://www.ebsciences.com/papers/mw_tech.htm A couple old books: The Microwave Cookbook for Microscopists, Boon and Kok Microwave Applications in Pathology [Hardcover] Anthony S. -Y Leong This person also wrote a lot of articles back in the 1980's and '90s on the subject Also a book by Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Monday, January 13, 2014 10:56 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave processing Hi! Can someone recommend literature about microwave processing. I'm interested in the physical principles behind the process. And I want to get answers to the questions: why is this microwave-assisted infiltration faster? What happens to proteins /antigens under microwave radiation? Is there a difference between conventional or microwave processing in relation to antigen preservation after usual formalinfixation. Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] microwave processing
Hi Gudrun: I recommend you to get The Microwave tool book by Login and Dvorak (1994) I am also sending you under separate cover an article I wrote on the subject. As to your questions, the practice of histology has concluded that: 1- the physical principle is that microwaves excite (shake) all chemical molecules with electrical charge and, in consequence, that shaking produces heat. That is why paraffin and any non-polar molecule cannot be heated in a MW oven per se. 2- infiltration is faster because the heat is generated within the tissues, not by external convection 3- proteins (and antigens as proteins themselves) are not adversely affected by MW radiation (or so the say). 4- everybody using MW tissue processing claims that IHC procedures are not affected by the procedure. Having said all of the above I personally do not like MW processing; there are many ways of having fast processing with conventional tissue processors. René J. From: Gudrun Lang gu.l...@gmx.at To: Histonet@lists.utsouthwestern.edu Sent: Monday, January 13, 2014 1:56 PM Subject: [Histonet] microwave processing Hi! Can someone recommend literature about microwave processing. I'm interested in the physical principles behind the process. And I want to get answers to the questions: why is this microwave-assisted infiltration faster? What happens to proteins /antigens under microwave radiation? Is there a difference between conventional or microwave processing in relation to antigen preservation after usual formalinfixation. Thanks in advance Gudrun Lang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Buehler Isomet 2000 Precision Saw or later model
Actively looking to purchase - possibly two, in good condition. Thanks Vicki @ 608-262-8534 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] myeloperoxidase on FFPE or frozen sections
Yep, We use a rtu from Leica on the Bond3 platform (PA0491) with EDTA (high pH) antigen retrieval Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mesru T Sent: Tuesday, 14 January 2014 5:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] myeloperoxidase on FFPE or frozen sections Dear Histonetters, I wonder if anyone has done myeloperoxidase stain on FFPE or frozen lung sections? I would appreciate any help. Regards, Mesru ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Recuts/deepers
Hello histonetters! Could I please get some help/advice/ideas on how to reduce recut/deeper requests? What does your lab do to reduce that? Would you say embedding is partially the problem? Such as small tissue embedded with bigger tissue? Etc? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Recuts/deepers
What are the reasons they ask for recuts? The answer to that will give you some ideas. For instance, did they not get a full face with margins the first time? Was the section not readable? Was the stain inadequate? Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Trini Sent: Monday, January 13, 2014 4:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Recuts/deepers Hello histonetters! Could I please get some help/advice/ideas on how to reduce recut/deeper requests? What does your lab do to reduce that? Would you say embedding is partially the problem? Such as small tissue embedded with bigger tissue? Etc? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
