[Histonet] question about refrigeration
Histonetters, I was wondering how long a bone specimen (portion of pt's tibia in this case) can be in a refrigerator before it will decompose and not produce a good quality section? The case was completed at 3:30 pm, refrigerated and then put into formalin at 8:15 am the next day? I am pushing to put specimen into formalin immediately after surgery. Thank you. Peggy DiCarlo HT (ASCP) Ortho Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida HealthÂs Technology Assistance Center at (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Slides for control/patient
Happy Friday! I was wondering, for the labs that are using the slides for IHC that have a place for the control tissue and the patient tissue, which vendor would you recommend. I also would not mind being contacted directly by vendors who wish to inform me of their products. Thanks, Toni ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Slides for control/patient
We like Fisher's Red Control Slide with Adhesion #22-042-910 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rathborne, Toni Sent: Friday, February 28, 2014 9:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Slides for control/patient Happy Friday! I was wondering, for the labs that are using the slides for IHC that have a place for the control tissue and the patient tissue, which vendor would you recommend. I also would not mind being contacted directly by vendors who wish to inform me of their products. Thanks, Toni ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Input on Sakura E150 Purchase
Another +1 for the VIP E300 (very similar model). We have one that's close to 20 years old that's still running. It has an annual PM and any repairs have been minor. I have been told that part availability isn't yet an issue with this model. Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: margaret chadwell [mailto:m_chadw...@hotmail.com] Sent: Wednesday, February 26, 2014 4:57 PM To: histonet@lists.utsouthwestern.edu; histonet-requ...@lists.utsouthwestern.edu Subject: [Histonet] Input on Sakura E150 Purchase Sent from Windows Mail Hello Histonet, Does anyone have input on the Sukura E150 tissue processor? Our research lab is looking at purchasing a used model from Rankin Biomedical, but we were concerned about the age of the unit, 17 years old. They mentioned it is totally refurbished, will give a one year warranty, say it’s a reliable unit, and parts are available for servicing. Any info or suggestions would be greatly appreciated. Like so many we are on a limited budget so we can’t buy new. Thank you in advance! Margaret Chadwell LIAI La Jolla, Ca _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Wierd hemotoxylin result on my macaque liver slide
Hi everyone. I was staining a FFPE macaque liver tissue slide with hematoxylin the other day, and it had an odd appearance. All the cells stained the usual way with the blue nuclear staining that you expect with hematoxylin. But a few randomly scattered individual cells throughout the tissue stained a sharp nuclear dark black. The cells are not clustered in any way, the cells have sharp black nuclear staining. It's definitely not artifact. Its not a cluster of lymphoid cells like you would see in inflammation. What would cause seemingly random individual cells scattered throughout the tissue to stain black with just hematoxylin? Just curious Patrick. CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Mouse NK cell marker
Good day, colleagues! Does anyone have a good Natural Killer cell marker for mouse tissue? Any antibody recommendations/protocol information would be a big help as well. This is for IHC, not IF. Thanks in advance! Ashley Troutman BS, MBA, HT(ASCP)QIHC Supervisor-Translational Pathology Shared Resource Vanderbilt University Medical Center S-1310 Medical Center North 1161 21st Avenue South Nashville, TN 37232 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] question about refrigeration
Hi Peggy, In my opinion, it depends upon the objective of the study. Cell autolysis begins shortly after death as a result of lack of oxygen supply as well as nutrients. The membranes of the lysosomes break down, and the acid hydrolases begin to degrade the cellular If you're merely looking to do a macro analysis, such as the quantitation of bone, you should be fine. However, if you're hoping to get results at the cellular level, either with traditional stains or IHC which relies on intact protein structure, you should immerse the specimens immediately after retrieval into formaldehyde solution. Good luck. Best regards, Sean Sean McBride AFIRM Project Leader Senior Scientific Specialist Research Histology Services Bone Tissue Engineering Center Carnegie Mellon Research Institute Suite 4311 700 Technology Drive Pittsburgh, PA 15219-3124 571-989-BONE (m) 412-268-8275 (o) 412-268-8275 (fax) smcbr...@andrew.cmu.edu On Feb 28, 2014, at 9:12 AM, DiCarlo, Margaret wrote: Histonetters, I was wondering how long a bone specimen (portion of pt's tibia in this case) can be in a refrigerator before it will decompose and not produce a good quality section? The case was completed at 3:30 pm, refrigerated and then put into formalin at 8:15 am the next day? I am pushing to put specimen into formalin immediately after surgery. Thank you. Peggy DiCarlo HT (ASCP) Ortho Bone Lab Buffalo General Hospital Buffalo, NY 14203 716-859-1293 The Keeping You Informed section of Kaleida Health`s website features a wealth of information, stories and pictures about our valued workforce and the tremendous momentum our organization is experiencing. Check us out at: www.kaleidahealth.org/kyi CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please call Kaleida Healths Technology Assistance Center at (716) 859-. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Input on Sakura E150 Purchase
We have a Sakura tissue processor that is more than 25 years old and still working very well with annual preventative maintenance. Lynn M Burton Histology Animal Disease Lab Galesburg, Il 309-344-2451 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dessoye, Michael J Sent: Friday, February 28, 2014 10:00 AM To: margaret chadwell; histonet@lists.utsouthwestern.edu; histonet-requ...@lists.utsouthwestern.edu Subject: RE: [Histonet] Input on Sakura E150 Purchase Another +1 for the VIP E300 (very similar model). We have one that's close to 20 years old that's still running. It has an annual PM and any repairs have been minor. I have been told that part availability isn't yet an issue with this model. Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: margaret chadwell [mailto:m_chadw...@hotmail.com] Sent: Wednesday, February 26, 2014 4:57 PM To: histonet@lists.utsouthwestern.edu; histonet-requ...@lists.utsouthwestern.edu Subject: [Histonet] Input on Sakura E150 Purchase Sent from Windows Mail Hello Histonet, Does anyone have input on the Sukura E150 tissue processor? Our research lab is looking at purchasing a used model from Rankin Biomedical, but we were concerned about the age of the unit, 17 years old. They mentioned it is totally refurbished, will give a one year warranty, say it’s a reliable unit, and parts are available for servicing. Any info or suggestions would be greatly appreciated. Like so many we are on a limited budget so we can’t buy new. Thank you in advance! Margaret Chadwell LIAI La Jolla, Ca _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Commonwealth Health. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] herbert skip brown
Skip, Please reply to me... Tim Morken Supervisor, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Bone Histology Protocol
Hello colleagues! I'm currently trying to construct a complete Bone Processing Protocol that includes fixation (10%NBF), decalcification (EDTA), Dehydration, Clearing of the decalcificant (Xylene), infiltration, and Embedding in Paraffin. I would like to look at some procedures just to get a good backbone of what a complete procedure is displayed like and I was hoping somebody could give me a website or a source where I could see some. I'm kind of new to bone histological processing so any procedures that are reliable will help! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate Abilene Christian University Class of 2013 Graduate B.S. Biochemistry ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
