[Histonet] RE: Xylene testing ANP 08216
All of my labs are tested at least yearly or when we change a major procedure dealing with formalin or xylene. In addition if an associate feels the fumes are high we test that day. When I CAP inspect I like to see the lab tests annually, however, it's not the requirement but would recommend as a caution to demonstrating a safe environment. Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafrini...@ccplab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, April 15, 2014 11:41 AM To: Piche, Jessica; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Xylene testing ANP 08216 We test every year. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica Sent: Tuesday, April 15, 2014 9:33 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Xylene testing ANP 08216 Hey Everyone, Just curious what people are doing with xylene testing? Are people really only doing the initial testing and never testing again if all was good? Seems like there should be periodic testing after decades have gone by! Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC imaging systems
I am setting up the Ventana system and performing validation, it's taking a little longer to set up then expected but the support has been excellent. Hopefully will have it running and validated in the next few months. Validation is the time consumer I found with setting up a system. Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafrini...@ccplab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 15, 2014 12:59 PM To: HistoNet Subject: [Histonet] IHC imaging systems Hello out there in Histoland, If any of you out there in the clinical environment are using imaging systems to quantify IHC slides, please let me know what system you are using. We are currently using a system that was provided through Dako, but they no longer support it. So time to consider buying a new one. It needs to have an algorithm that allows the system to distinguish between labelled and not labelled cells and the percentage of each. Thanks in advance, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] IHC imaging systems
Amen... the time investment for validation! Celebrate when it is completed :) Joelle Weaver MAOM, HTL (ASCP) QIHC From: michael.lafrini...@ccplab.com To: pat...@gmail.com; histonet@lists.utsouthwestern.edu Date: Thu, 17 Apr 2014 12:57:37 + Subject: RE: [Histonet] IHC imaging systems CC: I am setting up the Ventana system and performing validation, it's taking a little longer to set up then expected but the support has been excellent. Hopefully will have it running and validated in the next few months. Validation is the time consumer I found with setting up a system. Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafrini...@ccplab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, April 15, 2014 12:59 PM To: HistoNet Subject: [Histonet] IHC imaging systems Hello out there in Histoland, If any of you out there in the clinical environment are using imaging systems to quantify IHC slides, please let me know what system you are using. We are currently using a system that was provided through Dako, but they no longer support it. So time to consider buying a new one. It needs to have an algorithm that allows the system to distinguish between labelled and not labelled cells and the percentage of each. Thanks in advance, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Waste Flammable Info Please
I found each State that I have been in has a little different method to hazardous waste handling/reporting in the laboratory. I have found that recycling alcohol, xylene and formalin greatly reduces cost; my labs demonstrate 60-70% cost reduction in all areas of concern, (usage, disposal and regulation costs). However, you must invest time for a successful program, that includes ample space, training and monitoring to assure its maximum benefit. In today's environment of decreasing laboratory reimbursement this is a major LEAN process that can be highly valuable in the pathology laboratory! Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafrini...@ccplab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Tuesday, April 15, 2014 1:21 PM To: Dennis Hahn; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: Waste Flammable Info Please Dennis First of all we are in Colorado. If you dispose of more than 650 gallons a year in waste you are considered a small quantity generator, that amounts to one 55 gallon drum a month. Once you become a SQG this requires being registered with the EPA, additional safety training, every other year on site audits (In Colorado the Colorado Department of Public Health takes care of this) and then the other year a self-certification check list needs to be completed. If you dispose of less than 650 gallons you are considered a conditionally exempt small quantity generator and are not required to be registered with the EPA. Since you are disposing of 1 to 2 - 55 gallon drums a month that would mean that you would need to decrease your waste by at most 660 gallons a year or by 50%. When we recycled only alcohol in 2013 it looked like we still had around 1 - 55 gallon of waste per month. In 2014 We started recycling alcohol, xylene and proper, so far this year we have only had 2 - 55 gallons of waste picked up, that accounts for a 50% decrease in total waste, so it might be possible for you to start recycling and decrease your waste stream by 50%. We have also seen a cost saving in the purchasing of both alcohol and xylene the amount we purchase has decreased I don't have the specifics on the amount I just know we order less often that we used to. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dennis Hahn Sent: Tuesday, April 15, 2014 10:55 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Waste Flammable Info Please I have an inquiry specifically for Texas and/or Children's hospitals: What is your current volume of waste flammables and how do you handle it? Are you recycling, or is it removed by an outside vendor? A combination of the two? Suggestions on recycling instrumentation? Any drawbacks? We currently collect all waste flammable materials into a 55 Gallon drum and it is removed once or twice a month by a contracted vendor. The problem is, we are disposing of so much now that the vendor is requiring the medical center to obtain a high-use license involving the state and the EPA. We have evaluated recycling many years ago and found the process to be slow and sort of cumbersome. I know that the process has improved quite a bit over the years, but what about the tech time, tech exposure, time for recycling, etc.? Have you found it to be highly cost effective? Thanks in advance for any info you can share. Dennis Dennis Hahn, HT (ASCP) Histology Lab Supervisor Cook Children's Medical Center 801 7th Avenue Ft. Worth, TX 76104 (682) 885-6133 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] human cells in rat tissue
Hi, good afternoon everyone, I was wondering if anybody could help me out. We would like to detect human cells engrafted in rat tissue, particularly in formalin-fixed, paraffin-embedded tissue. I have so far tried one anti-human nuclear antigen antibody that works well in criostat...but without good results in paraffin. Do you know any anti human nuclear antibody that works well in paraffin? Or...do you know any other protein that let me differentiate between human cells and rat tissue? Obviously my cells are not GFP positive... Thanks a lot! Mercè Martí Gaudes Histology Unit P Abans d'imprimir aquest missatge, si us plau, comprova que és realment necessari. El medi ambient és cosa de tots. La informació continguda en aquest missatge i en qualsevol fitxer adjunt és confidencial, privada i d'ús exclusiu per al destinatari. Si no és la persona a la qual anava dirigida aquesta informació, si us plau, notifiqui immediatament l'enviament erroni al remitent i esborri el missatge. Qualsevol còpia, divulgació, distribució o utilització noautoritzada d'aquest correu electrònic i dels seus adjunts està prohibida en virtut de la legislació vigent. P Antes de imprimir este mensaje, por favor, comprueba que es realmente necesario. El medio ambiente es cosa de todos. La información contenida en este mensaje y en cualquier fichero adjunto es confidencial, privada y de uso exclusivo para el destinatario. Si usted no es la persona a la cual iba dirigida esta información, por favor, notifique inmediatamente el envio erróneo al remitente y borre el mensaje. Cualquier copia, divulgación, distribución o utilización no autorizada de este correo electrónico y de sus adjuntos está prohibida en virtud de la legislación vigente. P Before printing this e-mail, please make certain it is absolutely necessary. The environment is everybody's business. The information included in this e-mail and any attached files is confidential and private. If you are not the intended recipient, please notify the sender and delete this message immediately. Dissemination, forwarding or copying of this e-mail and its associated attachments is strictly prohibited in accordance with current legislation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: human cells in rat tissue
Merce There is another Human Nuclear antibody from cell signaling we find that it works in FFPE tissues, however in our hands it did not stain all nuclei probably around 90- 95% overall. We found it to be potentially fixation dependent, we noticed that tissues that had spent longer time in fixative did not stain that well and we also saw some staining in some rat samples, we could not figure out if it was due to our protocol or tissue collection but we decided that there was too many inconsistencies so we did not use it for that particular study. We might try it again. We do use another antibody to detect human cells in a rat background and that is HLA-1 the one we use is from abcam. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marti Gaudes, Merce Sent: Thursday, April 17, 2014 8:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] human cells in rat tissue Hi, good afternoon everyone, I was wondering if anybody could help me out. We would like to detect human cells engrafted in rat tissue, particularly in formalin-fixed, paraffin-embedded tissue. I have so far tried one anti-human nuclear antigen antibody that works well in criostat...but without good results in paraffin. Do you know any anti human nuclear antibody that works well in paraffin? Or...do you know any other protein that let me differentiate between human cells and rat tissue? Obviously my cells are not GFP positive... Thanks a lot! Mercè Martí Gaudes Histology Unit P Abans d'imprimir aquest missatge, si us plau, comprova que és realment necessari. El medi ambient és cosa de tots. La informació continguda en aquest missatge i en qualsevol fitxer adjunt és confidencial, privada i d'ús exclusiu per al destinatari. Si no és la persona a la qual anava dirigida aquesta informació, si us plau, notifiqui immediatament l'enviament erroni al remitent i esborri el missatge. Qualsevol còpia, divulgació, distribució o utilització noautoritzada d'aquest correu electrònic i dels seus adjunts està prohibida en virtut de la legislació vigent. P Antes de imprimir este mensaje, por favor, comprueba que es realmente necesario. El medio ambiente es cosa de todos. La información contenida en este mensaje y en cualquier fichero adjunto es confidencial, privada y de uso exclusivo para el destinatario. Si usted no es la persona a la cual iba dirigida esta información, por favor, notifique inmediatamente el envio erróneo al remitente y borre el mensaje. Cualquier copia, divulgación, distribución o utilización no autorizada de este correo electrónico y de sus adjuntos está prohibida en virtud de la legislación vigente. P Before printing this e-mail, please make certain it is absolutely necessary. The environment is everybody's business. The information included in this e-mail and any attached files is confidential and private. If you are not the intended recipient, please notify the sender and delete this message immediately. Dissemination, forwarding or copying of this e-mail and its associated attachments is strictly prohibited in accordance with current legislation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Billing Question
Hi, Please I have a question on the new IHC Billing Policies for CPT, Medicare. My question is how do we go about the billing of the HER-2 DUAL ISH and the Kappa and Lambda ISH? Thanks, Banjo Adesuyi, BSMT, HT (ASCP) HTL, QIHC, QLS Histology Supervisor Norman Regional Health System, Norman, OK 73071. Tel: 405- 307- 1145 == CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution, or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender immediately and destroy all copies of this communication and any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Billing Question
ISH is a different bill-code--88365. The changes were made to 88342. Donna Millard Director of Anatomic Pathology Physicians Reference Laboratory, LLC 7800 W. 110th Street,Overland Park, KS 66210 Direct: 913-339-0485 Fax: 913-319-4156 CONFIDENTIALITY NOTICE This message and any included attachments are from Physicians Reference Laboratory, LLC and are intended only for the addressee.The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call PRL's corporate offices in Overland Park, Kansas, U.S.A at (913)338-4070 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: human cells in rat tissue
We use an anti-human mitochondria antibody that works well. Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, April 17, 2014 10:53 AM To: Marti Gaudes, Merce; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: human cells in rat tissue Merce There is another Human Nuclear antibody from cell signaling we find that it works in FFPE tissues, however in our hands it did not stain all nuclei probably around 90- 95% overall. We found it to be potentially fixation dependent, we noticed that tissues that had spent longer time in fixative did not stain that well and we also saw some staining in some rat samples, we could not figure out if it was due to our protocol or tissue collection but we decided that there was too many inconsistencies so we did not use it for that particular study. We might try it again. We do use another antibody to detect human cells in a rat background and that is HLA-1 the one we use is from abcam. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marti Gaudes, Merce Sent: Thursday, April 17, 2014 8:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] human cells in rat tissue Hi, good afternoon everyone, I was wondering if anybody could help me out. We would like to detect human cells engrafted in rat tissue, particularly in formalin-fixed, paraffin-embedded tissue. I have so far tried one anti-human nuclear antigen antibody that works well in criostat...but without good results in paraffin. Do you know any anti human nuclear antibody that works well in paraffin? Or...do you know any other protein that let me differentiate between human cells and rat tissue? Obviously my cells are not GFP positive... Thanks a lot! Mercè Martí Gaudes Histology Unit P Abans d'imprimir aquest missatge, si us plau, comprova que és realment necessari. El medi ambient és cosa de tots. La informació continguda en aquest missatge i en qualsevol fitxer adjunt és confidencial, privada i d'ús exclusiu per al destinatari. Si no és la persona a la qual anava dirigida aquesta informació, si us plau, notifiqui immediatament l'enviament erroni al remitent i esborri el missatge. Qualsevol còpia, divulgació, distribució o utilització noautoritzada d'aquest correu electrònic i dels seus adjunts està prohibida en virtut de la legislació vigent. P Antes de imprimir este mensaje, por favor, comprueba que es realmente necesario. El medio ambiente es cosa de todos. La información contenida en este mensaje y en cualquier fichero adjunto es confidencial, privada y de uso exclusivo para el destinatario. Si usted no es la persona a la cual iba dirigida esta información, por favor, notifique inmediatamente el envio erróneo al remitente y borre el mensaje. Cualquier copia, divulgación, distribución o utilización no autorizada de este correo electrónico y de sus adjuntos está prohibida en virtud de la legislación vigente. P Before printing this e-mail, please make certain it is absolutely necessary. The environment is everybody's business. The information included in this e-mail and any attached files is confidential and private. If you are not the intended recipient, please notify the sender and delete this message immediately. Dissemination, forwarding or copying of this e-mail and its associated attachments is strictly prohibited in accordance with current legislation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4355 / Virus Database: 3882/7356 - Release Date: 04/17/14 - No virus found in this message. Checked by AVG - www.avg.com Version: 2014.0.4355 / Virus Database: 3882/7331 - Release Date: 04/11/14 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: human cells in rat tissue
We have used that too and it will work well in most instances, but there are some issues with that method, detection is entirely dependent upon the number of mitochondria in the cells and some cells contain more than others, as long you understand that you are fine. We feel we get a better staining reaction with the HLA-1 verses the mitochondria antibody. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: Mass Histology [mailto:jsta...@masshistology.com] Sent: Thursday, April 17, 2014 11:46 AM To: Elizabeth Chlipala; 'Marti Gaudes, Merce'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: human cells in rat tissue We use an anti-human mitochondria antibody that works well. Jim ___ James E. Staruk HT(ASCP) www.masshistology.com www.nehorselabs.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Thursday, April 17, 2014 10:53 AM To: Marti Gaudes, Merce; Histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: human cells in rat tissue Merce There is another Human Nuclear antibody from cell signaling we find that it works in FFPE tissues, however in our hands it did not stain all nuclei probably around 90- 95% overall. We found it to be potentially fixation dependent, we noticed that tissues that had spent longer time in fixative did not stain that well and we also saw some staining in some rat samples, we could not figure out if it was due to our protocol or tissue collection but we decided that there was too many inconsistencies so we did not use it for that particular study. We might try it again. We do use another antibody to detect human cells in a rat background and that is HLA-1 the one we use is from abcam. Good Luck Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marti Gaudes, Merce Sent: Thursday, April 17, 2014 8:41 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] human cells in rat tissue Hi, good afternoon everyone, I was wondering if anybody could help me out. We would like to detect human cells engrafted in rat tissue, particularly in formalin-fixed, paraffin-embedded tissue. I have so far tried one anti-human nuclear antigen antibody that works well in criostat...but without good results in paraffin. Do you know any anti human nuclear antibody that works well in paraffin? Or...do you know any other protein that let me differentiate between human cells and rat tissue? Obviously my cells are not GFP positive... Thanks a lot! Mercè Martí Gaudes Histology Unit P Abans d'imprimir aquest missatge, si us plau, comprova que és realment necessari. El medi ambient és cosa de tots. La informació continguda en aquest missatge i en qualsevol fitxer adjunt és confidencial, privada i d'ús exclusiu per al destinatari. Si no és la persona a la qual anava dirigida aquesta informació, si us plau, notifiqui immediatament l'enviament erroni al remitent i esborri el missatge. Qualsevol còpia, divulgació, distribució o utilització noautoritzada d'aquest correu electrònic i dels seus adjunts està prohibida en virtut de la legislació vigent. P Antes de imprimir este mensaje, por favor, comprueba que es realmente necesario. El medio ambiente es cosa de todos. La información contenida en este mensaje y en cualquier fichero adjunto es confidencial, privada y de uso exclusivo para el destinatario. Si usted no es la persona a la cual iba dirigida esta información, por favor, notifique inmediatamente el envio erróneo al remitente y borre el mensaje. Cualquier copia, divulgación, distribución o utilización no autorizada de este correo electrónico y de sus adjuntos está prohibida en virtud de la legislación vigente. P Before printing this e-mail, please make certain it is absolutely necessary. The environment is everybody's business. The information included in this e-mail and any attached files is confidential and private. If you are not the intended recipient, please notify the sender and delete this message immediately. Dissemination, forwarding or copying of this e-mail and its associated attachments is strictly prohibited in accordance with current
[Histonet] Xylene substitute for processing GI biopsies
Hi there! I have spent a lot of time reading the archives, but still need some specific and current input/advice. I'm interested in overnight processing for GI biopsies on a Tissue-Tek VIP with a xylene substitute. I need insight on protocols, brands, effects on staining. The good, the bad, the ugly. I have only used xylene in my many years of experience! This is completely new (to me), and rather overwhelming. It would be ideal to use the same substitute in the staining process, so any consideration with that is also appreciated. I understand this has been discussed, at length in the past, and I apologize I am just hoping to zero in on the best case scenario as a starting point. Thanks, in advance, for your help! Lori ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Histonet Digest, Vol 125, Issue 20
Hi Gayle, i saw your response to Merissa, quite good. i have also been having problems with lectin IHC and will appreciate it if you can also forward me your Lectin IHC protocol, and your recipe for the Lectin buffer. Thank you Regards. Daniel Daniel Osiagwu, MS Dept. of Anatomic and Molecular Pathology College of Medicine. University of Lagos. +2348188225457 dosia...@unilag.edu.ng - Original Message - From: histonet-requ...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Thursday, April 17, 2014 5:49:21 PM Subject: Histonet Digest, Vol 125, Issue 20 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. RE: Staining FFPE with biotinylated SNA - how to block? (James Watson) 2. Tracking control blocks (Fawn Bomar) 3. RE: Staining FFPE with biotinylated SNA - how to block? (gayle callis) 4. RE: Tracking control blocks (Cartun, Richard) 5. RE: Xylene testing ANP 08216 (Michael LaFriniere) 6. RE: IHC imaging systems (Michael LaFriniere) 7. RE: IHC imaging systems (joelle weaver) 8. RE: Waste Flammable Info Please (Michael LaFriniere) 9. human cells in rat tissue (Marti Gaudes, Merce) 10. RE: human cells in rat tissue (Elizabeth Chlipala) 11. Billing Question (Adesupo, Adesuyi (Banjo)) -- Message: 1 Date: Wed, 16 Apr 2014 18:15:58 + From: James Watson jwat...@gnf.org Subject: [Histonet] RE: Staining FFPE with biotinylated SNA - how to block? To: koelli...@comcast.net koelli...@comcast.net, M.O. modz9...@gmail.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: a032b25305ec6949ac9aff23c9620616c9f04...@ex5.lj.gnf.org Content-Type: text/plain; charset=utf-8 With our lectin staining on paraffin embedded tissue we use Avidin/Biotin blocking prior to antibody application. Some protocols we do use HIER before the blocking. One important item is that some lectins require a specific diluent. The dilution can be as high as 1:12,000. Lectins are well known for binding with multiple tissue entities, sometimes you can reduce the staining on unwanted tissue entities by reducing your incubation time or dilution. We do not use serum blocking. For PNA we use: PNA Diluent Calcium Chloride�..� 0.0111 gm. Magnesium Chloride��� 0.0203 gm. Manganese Chloride��� 0.0125 gm. Distilled Water�.���. 100.0 ml James Watson HT�� ASCP GNF�� Genomics Institute of the Novartis Research Foundation Tel�� 858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of koelli...@comcast.net Sent: Wednesday, April 16, 2014 9:53 AM To: M.O. Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Staining FFPE with biotinylated SNA - how to block? Hi Merissa, don't know if you got any private idea responses so I'll throw in my opinion.�� I would always worry about some of the things you are mentioning and that are standard thoughts regarding biotin block, retrieval, etc in IHC. But I would think about your serum, which I steadfastly avoided with SNA or any lectin I used. Lectins look at glyco components and serum (or serum substitutes) can be full of glycoproteins and the target then is the blocking serum for your lectin which can cause bad background.�� I did and would use washes, diluents, etc that had NO serum or milk or anything like that in them.�� You can make your own, completely free of potentially having��glycoproteins or Vector sells some.�� For some lectins (look at a list of target sugars) you maybe can get by with serum or milk and such to block��but many I've found you just can't. Ray (still in, whoever would have guessed, once again rainy Seattle) - Original Message - From: M.O. modz9...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Tuesday, April 15, 2014 5:59:51 PM Subject: [Histonet] Staining FFPE with biotinylated SNA - how to block? Hello Histonet! I ran a trial on FFPE mouse samples with a biotinylated lectin, SNA from vector. ��The SNA is Biotinylated Sambucus Nigra Lectin (Elderberry). ��I have never stained with anything like this, so I ran a test. I deparaffinized, blocked with
