RE: [Histonet] HER2
We use Integrated Oncology-Lab Corp, 521 West 57th Street, 6th floor, New York, NY 10019 800-447-5816 Josie Britton HT (ASCP) QIHC Cheshire Medical Center 580 Court Street Keene, NH 03431 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, May 13, 2014 6:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HER2 Hi Histonetters I am looking to compile a list of potential sites do send out Her2 slides for correlation on a validated assay. I have the Bond, using Refine detection. My clone is the Cb11, Oracle. Thanks in advance for any information you can provide. Joelle Weaver MAOM, HTL (ASCP) QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HER2
Thank you Josie this is very helpful. Joelle Weaver MAOM, HTL (ASCP) QIHC Subject: RE: [Histonet] HER2 Date: Wed, 14 May 2014 07:22:39 -0400 From: jcbrit...@cheshire-med.com To: joellewea...@hotmail.com; histonet@lists.utsouthwestern.edu We use Integrated Oncology-Lab Corp, 521 West 57th Street, 6th floor, New York, NY 10019 800-447-5816 Josie Britton HT (ASCP) QIHC Cheshire Medical Center 580 Court Street Keene, NH 03431 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Tuesday, May 13, 2014 6:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HER2 Hi Histonetters I am looking to compile a list of potential sites do send out Her2 slides for correlation on a validated assay. I have the Bond, using Refine detection. My clone is the Cb11, Oracle. Thanks in advance for any information you can provide. Joelle Weaver MAOM, HTL (ASCP) QIHC ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Liver Core Biopsies
Hello there! We are having some issues with our liver biopsies and can't seem to find any solutions. The biopsies are sent to us from IR already in formalin, we bake them in a 65* C oven for 30 minutes (to ensure fixation) and then we microwave process them. The microwave process is reagent alcohol, 5 mins, isopropanol, 5 mins, and finally paraffin, 2 min at 65* C and 5 min at 70* C. Worth noting: this is our procedure for all our biopsies - liver, heart, kidney, GI - and liver is the only one we are having problems with. The livers section well and don't appear to be over processed; however, without exception, they have a drying artifact on the outer edges. I have spoken to IR and they confirmed that they do not leave the biopsies out to dry for any amount of time. If they can't put them directly into formalin, the liver cores are held on saline soaked pads for no longer than a minute. I have seen suggestions about local anesthesia causing problems, but as we have no control over that and it's a necessary evil, I'd like to see if anyone has a suggestion to help minimize the artifact. Thanks! Merry Amber Lewis, HTL Children's Medical Center Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456- or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Scale or Balance
Hey Everyone, Does anyone know of a good brand/vendor for a balance (with a weighing dish) for the gross room? Thanks, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Scale or Balance
Mopec, did you try them? They make a lot of items for the gross room morgue. Joelle Weaver MAOM, HTL (ASCP) QIHC From: jpi...@wtbyhosp.org To: histonet@lists.utsouthwestern.edu Date: Wed, 14 May 2014 16:01:17 + Subject: [Histonet] Scale or Balance Hey Everyone, Does anyone know of a good brand/vendor for a balance (with a weighing dish) for the gross room? Thanks, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Large slide rack and adaptor for Sakura stainer
Hi all, Sakura has discontinued its large slide (50 x 75 mm) rack and metal adaptor for their Prisma stainer. Does anyone know a source for these that may have some left? Or do you have some you don't use anymore? The slide rack is a modified 24-slide Sakura rack that has cross bars on the bottom. The metal adaptors are inserts that slide in vertically on each end of the rack to space the slides. The catalog numbers from Sakura are: Large Slide Basket Sakura #4366 Adaptor for Large slides #4365 Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] **Position Alert** New Histology Opportunity
Good Morning! We have had a world leading cancer diagnostics client open a new opportunity on the West Coast (to be based in CA, OR or WA). Our client is currently searching for a talented histology professional with a strong background in the laboratory to join our clients field support team in the West. The position offers an outstanding opportunity to break into a manufacturing company, a very competitive package and excellent growth potential. Please contact me directly to learn more about the opportunity at m...@personifysearch.com mailto:m...@personifysearch.com or 800-875-6188 ext 103 Best Regards, Matt Ward Program Manager Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 919.459.3654 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 http://www.personifysearch.com/ www.personifysearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Scale or Balance
We have always liked Mettler-Toledo: http://www.lab-balance.com/finder/ Michael J. Dessoye, M.S. | Histology/Toxicology/RIA Supervisor | Wilkes-Barre General Hospital | An Affiliate of Commonwealth Health | mjdess...@commonwealthhealth.net | 575 N. River Street | Wilkes Barre, PA 18764 | Tel: 570-552-1432 | Fax: 570-552-1526 -Original Message- From: Piche, Jessica [mailto:jpi...@wtbyhosp.org] Sent: Wednesday, May 14, 2014 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Scale or Balance Hey Everyone, Does anyone know of a good brand/vendor for a balance (with a weighing dish) for the gross room? Thanks, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party unless required to do so by law or regulation. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this information in error, please notify the sender immediately and delete these documents. Copyright (c) Waterbury Hospital -- Disclaimer: This electronic message may contain information that is Proprietary, Confidential, or legally privileged or protected. It is intended only for the use of the individual(s) and entity named in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from your computer. Do not deliver, distribute or copy this message and do not disclose its contents or take any action in reliance on the information it contains. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Large slide rack and adaptor for Sakura stainer
I posted about this earlier this year. Apparently they are no longer available in the US. I have found no substitute for these racks or adapter and Sakura was unwilling to help in any way. (Which I am still miffed about). If someone has a metal shop, here is a great opportunity. Angela K. Bitting, HT (ASCP), QIHC Technical Specialist IHC/Special Staining Geisinger Medical Center 100 N. Academy Ave. Danville, PA 17822-2300 Tel.570-214-9634 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy Sent: Wednesday, May 14, 2014 2:01 PM To: Histonet Subject: [Histonet] Large slide rack and adaptor for Sakura stainer Hi all, Sakura has discontinued its large slide (50 x 75 mm) rack and metal adaptor for their Prisma stainer. Does anyone know a source for these that may have some left? Or do you have some you don't use anymore? The slide rack is a modified 24-slide Sakura rack that has cross bars on the bottom. The metal adaptors are inserts that slide in vertically on each end of the rack to space the slides. The catalog numbers from Sakura are: Large Slide Basket Sakura #4366 Adaptor for Large slides #4365 Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center Box 1656 505 Parnassus Ave San Francisco, CA 94143 USA 415.514-6042 (office) tim.mor...@ucsfmedctr.orgmailto:tim.mor...@ucsfmedctr.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Luxol Fast Blue
Hello everybody, can anybody send me a Luxol Fast Blue staining protocol for mouse/rat brain (40µm slices) and spinal cord (10µm)? Any tipps and tricks are higly appriciated!! Thank you Christina ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Luxol Fast Blue
There are several procedures online. Just google Luxol fast blue staining procedures. It is an easy stain to do. Andi Andrea Grantham, HT (ASCP) Senior Research Specialist University of Arizona Cellular and Molecular Medicine Histology Service Laboratory P.O.Box 245044 Tucson, AZ 85724 algra...@email.arizona.edumailto:algra...@email.arizona.edu Tel: 520.626.4415 Fax: 520.626.2097 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Scale or Balance
Jessica Piche, HT(ASCP), presumably at Waterbury Hospital in Connecticut, asks: Does anyone know of a good brand/vendor for a balance (with a weighing dish) for the gross room? You can get excellent top-loading electronic balances from Ohaus or Mettler or others, for two or three hundred dollars each. These are sold through the standard vendors like whatever Scientific Products is called this week. You must know exactly what you want, and I expect your pathologists to know this. Balance specifications change rapidly as manufacturers improve their products, so that catalog numbers won't help you much. For ordinary gross room use, you want a balance that will weigh in gram increments up to a kilogram or so, so you can weigh 10 grams of prostatic chips or 600 grams of placenta with decent precision and accuracy. For weighing parathyroid specimens, you need to be able to weigh in 10 mg increments, up to a few hundred or thousand milligrams. Weighing parathyroids is standard of care, and if your pathologists and your MBAs don't know it you need to tell them. That's going to take a second balance. (Tip - if your hospital pharmacy still does a little compounding, they may have the right balance. One of my hospitals keeps its old balance around, just for me.) Two caveats you wouldn't think of - unlike the platform balances of yore, electronic balances need replacing fairly often. And druggies will steal your balances to weigh out illegal drugs - in some venues, you have to lock your balances up when you're not using them. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Dil labeling
Hello all, Please I need some help in the lipophilic dil labeling of free floating brain sections. The mice brain were fixed in 1.5% PFA by cardia perfusion, then left one hour in the same fixative. I used the Dil labeling solution for a 200 um thick sections, the dilution was 1:150 in PBS. The sections were incubated for 48h in the Dil labeling solution then 48 in PBS in the cold room in the shaker. The problem that I had a very high background and I could not see even one spine. I need to stain only a few neurons so I can do a reasonable quantification of the size and number of the spines. Does anybody has an idea if in this case the solution or the crystal Dil is better and can I get an optimized protocol for this kind of staining. Thank you so much All the best Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Looking for Volunteers for NSH Career Day
Histotechs! Are you attending the NSH Annual Symposium in Austin this year? We are looking for a histotech that works in research doing whole mount sections to come and present at Career Day. If you work in research and love it, we want you :) It's a great way to share your knowledge, and you will receive CEU credits for it! If you are interested, feel free to email me under separate cover at mcoke...@gmail.com, or even better, email the Career Day coordinator Sue Clark at do...@bellsouth.net. Cheers!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
