[Histonet] Re: Histonet Digest, Vol 126, Issue 15
Hi Andi, I have a Google Doc of our procedure. I just sent you an invite to view it. If anyone else wants to see it, just let me know I'll share :-) I know the procedure calls for overnight in the LFB solution. It's for the best, the results are much better if you are patient. It is really important to not run the rehydration to water. End in 95% ETOH, the water will really kill the stain. Good luck, Amos On Thu, May 15, 2014 at 1:00 PM, histonet-requ...@lists.utsouthwestern.eduwrote: Message: 7 Date: Wed, 14 May 2014 19:23:58 + From: Grantham, Andrea L - (algranth) algra...@email.arizona.edu Subject: Re: [Histonet] Luxol Fast Blue To: Christina Kreutzer christina.kreutze...@gmail.com Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: cabfb32d-027a-4ee0-b8e9-6cab3261e...@email.arizona.edu Content-Type: text/plain; charset=us-ascii There are several procedures online. Just google Luxol fast blue staining procedures. It is an easy stain to do. Andi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] time frame on a CLIA application in colorado
Hello, I have been striking out on getting the local CLIA office to call me back I regards to some information. I am turning to you all in hopes someone in the Denver Colorado area can help me. I need to find out the time frame it takes from the time a CLIA application (high complexity lab and assuming all information is filled out correctly) is submitted to the time a coupon for payment is received in the mail? 3 months? 6 months? If anyone can help me find this information, I would appreciate it. Thanks, Gloria ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Help for identifying the blue stained structure
Hello, I have an embryonic sample that decalcified, paraffin embedded, and stained with Mallory Trichrome (Aniline Blue, Orange G, Acid Fuchsin). I will upload the image in the Histonet Images. This is a cranial region where the bone is being resorbed, so I expected to see the bone (dark blue in trabecular), and red blood, and adjacent white spaces that is being resorbed. Instead, there is a very uniform, granular structure stained with blue without any nuclei at the resorbed regions. This structure looks like almost crystal or some kind of secretion leakage from the ossifying bone. I need a help to identify this blue stained things. I don’t think this is osteoid, because at later stage embryos, there is no bone at this region. If you have some suggestions for other stain to identify this blue thing, or help identifying this, I really appreciate it. Thank you in advance, Rui ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
