[Histonet] positive control indoleamine 2-3 dioxygenase
Hi All, We are going to optimize indoleamine 2-3 dioxygenase (IDO1) expression in breast cancer tissue by immunohistochemistry on formalin fixed paraffin sections. I shall be thankful if any one please let me know about the positive control. Regards Muhammad Tahseen Sr.Supervisor Histopathology SKMCHRC Lahore Pakistan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] immunohistochemisty coding
Hi Sarah, Joyce is correct, under medicare changes in pathology coding, you can not bill for each of the blocks of the same antibody applications, only one charge per specific antibody mentioned in the pathology report is allowable. Medicare immuno billing is per specimen only for each specific antibody used, example if you used s100 for the same specimen (wide melanoma excision)on 15 different blocks, under medicare you can only bill one GO461, yes- not fair when the need is appropriate, I must voice my opinio)- this probably will lower some of the over utilization and inappropriate billing practices that many of us have seen in the immunohistochemistry world and the labs that have capitalized on the practice, the problem with the lower reimbursements and change in medicare coding hurts many of our labs who have used the utilization of immunohistochemistry appropriately. However, under current 2014 CPT coding on non medicare can you bill for the additional work per block using the 88342 and 88343 cpt code, I am sure moving forward we are going to continue to see adjustments to the pathology coding processes! Michael R. LaFriniere, HT (ASCP) Executive Director Capital Choice Pathology Laboratory 12041 Bournefield Way, Suite A * Silver Spring, MD 20904 P: 240.471.3427 * F: 240.471.3401 * Cell 410-940-8844 michael.lafrini...@ccplab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Monday, May 19, 2014 12:21 PM To: 'sarah.dys...@stdavids.com'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Billing No - one G0461. If you had AE1/AE3, Melan A, S100 you would charge G0461 x 1 and G0462 x 2. j Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah.dys...@stdavids.com Sent: Monday, May 19, 2014 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Billing When it comes to the medicare codes...question... So you have one specimen that has 10 blocks. AE1/AE3 is ordered on all 10 blocks. Can you bill AE1/AE3 1st Antibody once (G0461), then AE1/AE3 Additional (G0462) nine times? Thanks, Sarah E. Dysart, BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor St. David's North Austin Medical Center 12221 North Mopac Expressway Austin, Texas 78758 (512)901-1220 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] antibody vials
Does anyone have any good ideas for keeping the little vials of concentrated antibodies neat and organized in the fridge? Right now we keep ours in empty pipette tip boxes (without tip holders) but they're always falling over and mixed up. We use several different vendors for our concentrated antibodies, so our vials are all different sizes. Thanks for any ideas! Clare J. Thornton, HTL(ASCP)QIHC Lead Immunohistochemistry Technologist Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.commailto:cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: antibody vials
I use cardboard cryovial boxes and they work quite well. You can remove some of the dividers to make space for the larger vials. http://labscientific.com/Cryogenic-Supplies/Cryovial-Boxes-and-Freezer-Racks/Cryovial-Boxes-Cardboard/ Valerie Ratliff B.Sc HTL(ASCP) Research Assistant Department of Oncology Karmanos Cancer Institute 4100 John R Detroit, MI 48201 Telephone: (313) 576 8282 Fax: (313) 576 8306 E-mail: murp...@karmanos.org Better treatments. Better outcomes. From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Clare Thornton [cthorn...@dahlchase.com] Sent: Tuesday, May 20, 2014 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antibody vials Does anyone have any good ideas for keeping the little vials of concentrated antibodies neat and organized in the fridge? Right now we keep ours in empty pipette tip boxes (without tip holders) but they're always falling over and mixed up. We use several different vendors for our concentrated antibodies, so our vials are all different sizes. Thanks for any ideas! Clare J. Thornton, HTL(ASCP)QIHC Lead Immunohistochemistry Technologist Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.commailto:cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HELP- Cryosectioning FAT!
Hi all, I've been having problems with cryosectioning fatty tissue for a long time now. The section leaves a hole in the middle. I know this is probably a much discussed topic, but I've tried a lot of strategies with no luck. The tissue is of mouse origin and has micro-lesions buried inside fat. So the fat needs to be cut in order to get to the lesion. I started with a temperature of -17C and tried upto -25C and also at -50C; didn't work at any of these temperatures. I tried rubbing the block, blade and anti-roll plate with dry ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the block at -80C. Could I try sectioning the block directly from -80C? (never done that). Any other suggestions on how to tackle this? Any advice is much appreciated!! Thanks, Dakshna ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] new processor
Thanks everyone for your input on processors, I think we will stay with the VIP, it has been a good machine just getting older now. everyone have a great day Anita Dudley Providence Hosp Mobile, Alabama 36695 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] antibody vials
We got some plexiglass dividers at the Container Store if you have one near or check online. Can't remember what they were called but they had different sized square compartments that fit various venders antibodies. Anne -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Clare Thornton Sent: Tuesday, May 20, 2014 7:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antibody vials Does anyone have any good ideas for keeping the little vials of concentrated antibodies neat and organized in the fridge? Right now we keep ours in empty pipette tip boxes (without tip holders) but they're always falling over and mixed up. We use several different vendors for our concentrated antibodies, so our vials are all different sizes. Thanks for any ideas! Clare J. Thornton, HTL(ASCP)QIHC Lead Immunohistochemistry Technologist Dahl-Chase Diagnostic Services 417 State Street, Suite 540 Bangor, ME 04401 cthorn...@dahlchase.commailto:cthorn...@dahlchase.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HELP- Cryosectioning FAT!
Thanks Sandra! My next step was to try that. Dakshna - Original Message - From: Sandra E. Esparza sespa...@seton.org To: Dakshnapriya Balasubbramanian dakshnapr...@neo.tamu.edu Sent: Tuesday, May 20, 2014 10:34:25 AM Subject: RE: [Histonet] HELP- Cryosectioning FAT! You might want to dry Liquid Nitrogen on the face of the block before you cut it. Sandra Sandra Esparza HT (ASCP), QIHC Histotechnologist Mohs Austin Dermatologic Surgery Center 512-324-7468 x84027 sespa...@seton.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Balasubbramanian, Dakshnapriya Sent: Tuesday, May 20, 2014 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP- Cryosectioning FAT! Hi all, I've been having problems with cryosectioning fatty tissue for a long time now. The section leaves a hole in the middle. I know this is probably a much discussed topic, but I've tried a lot of strategies with no luck. The tissue is of mouse origin and has micro-lesions buried inside fat. So the fat needs to be cut in order to get to the lesion. I started with a temperature of -17C and tried upto -25C and also at -50C; didn't work at any of these temperatures. I tried rubbing the block, blade and anti-roll plate with dry ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the block at -80C. Could I try sectioning the block directly from -80C? (never done that). Any other suggestions on how to tackle this? Any advice is much appreciated!! Thanks, Dakshna ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HELP- Cryosectioning FAT!
Hi Laurie, No, the tissue hasn't been fixed before freezing. My prof doesn't prefer the method, but if nothing else works, we might give it a try. Any suggested protocols for fixation? Thanks! Dakshna - Original Message - From: Laurie J King king.lau...@marshfieldclinic.org To: Dakshnapriya Balasubbramanian dakshnapr...@neo.tamu.edu Sent: Tuesday, May 20, 2014 10:37:35 AM Subject: RE: [Histonet] HELP- Cryosectioning FAT! Dakshna, Has this tissue been fixed before freezing by any chance? laurie -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Balasubbramanian, Dakshnapriya Sent: Tuesday, May 20, 2014 10:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HELP- Cryosectioning FAT! Hi all, I've been having problems with cryosectioning fatty tissue for a long time now. The section leaves a hole in the middle. I know this is probably a much discussed topic, but I've tried a lot of strategies with no luck. The tissue is of mouse origin and has micro-lesions buried inside fat. So the fat needs to be cut in order to get to the lesion. I started with a temperature of -17C and tried upto -25C and also at -50C; didn't work at any of these temperatures. I tried rubbing the block, blade and anti-roll plate with dry ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the block at -80C. Could I try sectioning the block directly from -80C? (never done that). Any other suggestions on how to tackle this? Any advice is much appreciated!! Thanks, Dakshna ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __ The contents of this message may contain private, protected and/or privileged information. If you received this message in error, you should destroy the e-mail message and any attachments or copies, and you are prohibited from retaining, distributing, disclosing or using any information contained within. Please contact the sender and advise of the erroneous delivery by return e-mail or telephone. Thank you for your cooperation. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Pankeratin- Trouble with Mast Cell Staining
Posting for my colleague, Jeff Gordon, because his post did not go through successfully: Suzie, your histonet post was forwarded to me by a colleague, and I thought I could help with your mast cell staining dilemma with pan-keratin. University of Pennsylvania published an article a while back about undesirable reactivity of cytokeratins that focused primarily on sentinel lymph node testing. In their study they found that when cytokeratin markers OTHER than strictly AE1/AE3 (with no other components) were used, other non-epithelial elements were being labeled that they deemed undesirable. This entire article can be found here for your's and your pathologists' reference: http://www.archivesofpathology.org/doi/pdf/10.1043/0003-9985(2000)124%3C1310%3AUCIONN%3E2.0.CO%3B2 Based on these findings, and because another person in the thread also indicated that they may be dealing with the same issue, cervical tissue that was rich in mast cells was stained on a Ventana Ultra with cytokeratin AE1/AE3 ONLY as well as with cytokeratin OSCAR (which is another pan-keratin but with a lesser sensitivity to squamous epithelium than AE1/AE3) with CC1 mild heat retrieval and 16 minute heated primary incubation and Ultraview detection. To confirm the presence of mast cells in the tissue, both CD117 and tryptase were also stained on the same tissue with the same protocols. The results showed that though there was an abundance of mast cells present labeled by tryptase and CD117, there was no detectable staining of mast cells with either cytokeratin AE1/AE3 or with cytokeratin OSCAR, while the epithelial portion of the tissue stained with both cytokeratins (though the OSCAR was noticeably weaker because of the lower sensitivity to squamous epithelium). The stains can be seen here: http://www.cellmarque.com/cmc/AO_mastcellreactivity.php Hopefully this helps. If you have any questions, please contact us at your convenience. Thank you -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Miller, Suzie Sent: Tuesday, May 06, 2014 8:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pankeratin- Trouble with Mast Cell Staining Hello Everyone, If you have a Ventana Ultra and use (Ventana# 760-2595) Pankeratin AE1/AE3/PCK29, would you consider sharing your staining protocol? Our Pathologists are displeased with the amount of mast cell staining with our current protocol so we are looking to explore other protocols or suggestions. Thank you for your assistance, Suzie Suzie Miller, MLT ASCP Senior Histotech Mercy Health System of Maine Laboratory mill...@emhs.orgmailto:mill...@emhs.org --- This email message, including any associated files, is for the sole use of the intended recipient(s) and may contain information that is confidential, privileged, or subject to copyright, trade secret or other protection. This message also may contain information protected by state and federal privacy laws that are enforced through serious civil and criminal sanctions. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not an intended recipient of this message, please notify the sender immediately by replying to this e-mail, and delete the original and all copies of this message from your computer or other device. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Pankeratin- Trouble with Mast Cell Staining
We found that if using the stronger proteases/enzymes for retrievals, there tended to be more mast cell staining seen. Sheila Herrington Technical Lead - Histopathology and Immunohistochemistry Kelowna General Hospital 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2 250-862-4300 ext 7587 or 7510 sheila.herring...@interiorhealth.ca -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Daniel Gonzalez Sent: Tuesday, May 20, 2014 1:33 PM To: 'Miller, Suzie'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Pankeratin- Trouble with Mast Cell Staining Posting for my colleague, Jeff Gordon, because his post did not go through successfully: Suzie, your histonet post was forwarded to me by a colleague, and I thought I could help with your mast cell staining dilemma with pan-keratin. University of Pennsylvania published an article a while back about undesirable reactivity of cytokeratins that focused primarily on sentinel lymph node testing. In their study they found that when cytokeratin markers OTHER than strictly AE1/AE3 (with no other components) were used, other non-epithelial elements were being labeled that they deemed undesirable. This entire article can be found here for your's and your pathologists' reference: http://www.archivesofpathology.org/doi/pdf/10.1043/0003-9985(2000)124%3C1310%3AUCIONN%3E2.0.CO%3B2 Based on these findings, and because another person in the thread also indicated that they may be dealing with the same issue, cervical tissue that was rich in mast cells was stained on a Ventana Ultra with cytokeratin AE1/AE3 ONLY as well as with cytokeratin OSCAR (which is another pan-keratin but with a lesser sensitivity to squamous epithelium than AE1/AE3) with CC1 mild heat retrieval and 16 minute heated primary incubation and Ultraview detection. To confirm the presence of mast cells in the tissue, both CD117 and tryptase were also stained on the same tissue with the same protocols. The results showed that though there was an abundance of mast cells present labeled by tryptase and CD117, there was no detectable staining of mast cells with either cytokeratin AE1/AE3 or with cytokeratin OSCAR, while the epithelial portion of the tissue stained with both cytokeratins (though the OSCAR was noticeably weaker because of the lower sensitivity to squamous epithelium). The stains can be seen here: http://www.cellmarque.com/cmc/AO_mastcellreactivity.php Hopefully this helps. If you have any questions, please contact us at your convenience. Thank you -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Miller, Suzie Sent: Tuesday, May 06, 2014 8:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pankeratin- Trouble with Mast Cell Staining Hello Everyone, If you have a Ventana Ultra and use (Ventana# 760-2595) Pankeratin AE1/AE3/PCK29, would you consider sharing your staining protocol? Our Pathologists are displeased with the amount of mast cell staining with our current protocol so we are looking to explore other protocols or suggestions. Thank you for your assistance, Suzie Suzie Miller, MLT ASCP Senior Histotech Mercy Health System of Maine Laboratory mill...@emhs.orgmailto:mill...@emhs.org --- This email message, including any associated files, is for the sole use of the intended recipient(s) and may contain information that is confidential, privileged, or subject to copyright, trade secret or other protection. This message also may contain information protected by state and federal privacy laws that are enforced through serious civil and criminal sanctions. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not an intended recipient of this message, please notify the sender immediately by replying to this e-mail, and delete the original and all copies of this message from your computer or other device. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
