[Histonet] Storage of 10% NB formalin
My lab is having a discussion about the storage location of our prefilled NB 10% formalin in a stock room. These containers haven't been used or opened. Does anyone know what the current recommendations are for the safe storage of NB 10% formalin? I seem to recall something about an allowed total volume of formalin in relationship with the total area of a given space. Carolyn K. Barnes carolyn.barn...@va.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microwave Processor
I am looking to buy a NEW Microwave tissue processor. Could you please give me your pro and con for instruments available in your lab? Price is a big issue, if anyone knows about a cheap but very reliable I would like to hear about it PLEASE! Thank you very much. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) CTDDA Histology Lab Supervisor ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] AUTO: Bruce Palmatier is out of the office (returning 06/13/2014)
I am out of the office until 06/13/2014. I will be out of the office from June 10 through June 13. I will have limited access to email but will be responding as possible in the evening. Thank You, Bruce Palmatier Market Portfolio Manager VWR Healthcare bruce_palmat...@vwr.com mobile: 484.319.5563 fax: 484-881-7307 Customer Service: 877.881.1192 Fax: 484.881.6486 Customer Service email: healthcareserv...@vwr.com Note: This is an automated response to your message Histonet Digest, Vol 127, Issue 13 sent on 6/11/2014 7:00:09 AM. This is the only notification you will receive while this person is away. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microwave Processor
Look at the options presented by Milestone René J. On Wednesday, June 11, 2014 9:56 AM, Bustamante, Lin lbustama...@cvm.tamu.edu wrote: I am looking to buy a NEW Microwave tissue processor. Could you please give me your pro and con for instruments available in your lab? Price is a big issue, if anyone knows about a cheap but very reliable I would like to hear about it PLEASE! Thank you very much. Lin. Lin S. Bustamante, B.S., H.T.(ASCP) CTDDA Histology Lab Supervisor ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Request for Non-fluorescent Sealant
John, I was recommended and have used black nail polish for FISH applications without background. Mike -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paul Scott Sent: Tuesday, June 10, 2014 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Request for Non-fluorescent Sealant Hi John, My company SciGene has a product called CytoBond Removable Coverslip Sealant that was designed for use in FISH as replacement to rubber cement for temporarily sealing of the coverslips during probe hybridization. Customers tell us how much easier it is to remove as opposed to rubber cement. If you send me your details I can get a free sample out to you. It is usually completely removed (with no remainder left behind) during imaging so I cannot provide feedback as to its autoflouresence over a spectral range. Paul Scott Technical Sales Representative SciGene 470 Lakeside Drive, Ste F, Sunnyvale, CA 94085-4720 408-733-7337 x 305 psc...@scigene.com mailto:305psc...@scigene.com Automating FISH and CMA Workflows http://www.scigene.com/ www.SciGene.com Hello, I am wondering if anyone would know of a sealant that is non-fluorescent (or doesn't leak). I am using a biorad duo chamber slide which has two slots for each chamber, I need to measure the fluorescence of some sample but Rubber Cement and the nail polish I have tried have both had some interfering fluorescence. Any help is appreciated! -John ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Aperio slide scanner
My department may purchase an Aperio slide scanner in the near future. My question is - how tech savvy does one need to be to operate the device? I have staffing concerns and the amount of training time involved. Thanks in advance. -- Jan Shivers IHC/Histology Section Head Pathology Teaching Program University of Minnesota shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Aperio slide scanner
Jan They are not that difficult to operate but you do need person with some histology training scanning and QC'ing the slides and images. The need to understand initially if the section is good enough to scan, we QC our slides prior to placing them on the scanner, ones that have sectioning artifacts may not scan that well. Section placement on the slide is important, you can't have anything to close to the edge of a slide it will not scan well. The person responsible for scanning makes sure that the slide is clean and does not have any excess mounting media prior to placing on the scanner, excess mounting media and dirt may cause the scan to be out of focus, and then once the slides are scanned the scans need to be QC'd to make sure that they are good enough for the pathologist or whatever you are utilizing them for. Quality and consistency in histology preparation is key for good scanning results. These scanners scan in primarily one focal plane, meaning the pathologist loses the ability to fine focus as they would on a microscope. They are able to fine focus through poorer quality sections or uneven sections, scanned images do not have a fine focus unless you scan them at multiple focal planes or z-stack. I have seen some cytology images that have been scanned in a way that you have a fine focus slider but its not common for routine histology preps. We take some time upfront to adjust area of interest and check focal points prior to scanning of the slides. We find this works better than just going with the load and go method. We review the snapshots prior to scanning. I'm not sure what scanner you are getting or what version of the image capture software you will be using we have an Aperio ScanScope XT it has a 120 slide capacity. On occasion a particular slide may not scan well, that’s why we like to review the snapshots. For instance we were working on some amniotic membrane constructs these are a single cell layer thick so they are very thin and sometimes the computer does not pick the sample up as tissue because it so thin and can be lightly stained, so we need to place all of the focal points on the slides. You may not have samples like this but you might. Good Luck - feel free to contact me if you need any help once you get the scanner in house. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Wednesday, June 11, 2014 10:25 AM To: histonet Subject: [Histonet] Aperio slide scanner My department may purchase an Aperio slide scanner in the near future. My question is - how tech savvy does one need to be to operate the device? I have staffing concerns and the amount of training time involved. Thanks in advance. -- Jan Shivers IHC/Histology Section Head Pathology Teaching Program University of Minnesota shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Aperio slide scanner
Jan, We like ours a lot. And I agree with all that Liz mentioned. Bea Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Wednesday, June 11, 2014 9:25 AM To: histonet Subject: [Histonet] Aperio slide scanner My department may purchase an Aperio slide scanner in the near future. My question is - how tech savvy does one need to be to operate the device? I have staffing concerns and the amount of training time involved. Thanks in advance. -- Jan Shivers IHC/Histology Section Head Pathology Teaching Program University of Minnesota shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Aperio slide scanner
I will echo Liz's comments. I occasionally scan slides with an older Scanscope XT. It is worth the effort to clean and QC slides for artifacts as Liz points out as well as placing the section in the middle of the slide. Actual scanning is quite easy, each initial 'snapshot' is reviewed, AOI is resized to boundaries of the tissue and focus points are checked and verified to be overlaying tissue (not white space). I find that adding more focus points across the section greatly increased the quality of the image and rarely have I found any areas out of focus. Once the snapshot reviews are completed simply hit the 'One Touch' icon and batch scanning begins. I really like the ImageScope viewing program which lets one view the section and multiple magnifications, capture images, etc.. I have another system for IHC quantification, but other users are using the Aperio software to do that if that is part of your plan Brett Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, June 11, 2014 12:46 PM To: Jan Shivers; histonet Subject: RE: [Histonet] Aperio slide scanner Jan They are not that difficult to operate but you do need person with some histology training scanning and QC'ing the slides and images. The need to understand initially if the section is good enough to scan, we QC our slides prior to placing them on the scanner, ones that have sectioning artifacts may not scan that well. Section placement on the slide is important, you can't have anything to close to the edge of a slide it will not scan well. The person responsible for scanning makes sure that the slide is clean and does not have any excess mounting media prior to placing on the scanner, excess mounting media and dirt may cause the scan to be out of focus, and then once the slides are scanned the scans need to be QC'd to make sure that they are good enough for the pathologist or whatever you are utilizing them for. Quality and consistency in histology preparation is key for good scanning results. These scanners scan in primarily one focal plane, meaning the pathologist loses the ability to fine focus as they would on a microscope. They are able to fine focus through poorer quality sections or uneven sections, scanned images do not have a fine focus unless you scan them at multiple focal planes or z-stack. I have seen some cytology images that have been scanned in a way that you have a fine focus slider but its not common for routine histology preps. We take some time upfront to adjust area of interest and check focal points prior to scanning of the slides. We find this works better than just going with the load and go method. We review the snapshots prior to scanning. I'm not sure what scanner you are getting or what version of the image capture software you will be using we have an Aperio ScanScope XT it has a 120 slide capacity. On occasion a particular slide may not scan well, that’s why we like to review the snapshots. For instance we were working on some amniotic membrane constructs these are a single cell layer thick so they are very thin and sometimes the computer does not pick the sample up as tissue because it so thin and can be lightly stained, so we need to place all of the focal points on the slides. You may not have samples like this but you might. Good Luck - feel free to contact me if you need any help once you get the scanner in house. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Wednesday, June 11, 2014 10:25 AM To: histonet Subject: [Histonet] Aperio slide scanner My department may purchase an Aperio slide scanner in the near future. My question is - how tech savvy does one need to be to operate the device? I have staffing concerns and the amount of training time involved. Thanks in advance. -- Jan Shivers IHC/Histology Section Head Pathology Teaching Program University of Minnesota shive...@umn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice: This e-mail message, together with any attachments, contains information of Merck Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889),
RE: [Histonet] Aperio slide scanner
We load and scan the same way Brett does, it works quite well and we rarely have to rescan anything. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: Connolly, Brett M [mailto:brett_conno...@merck.com] Sent: Wednesday, June 11, 2014 11:39 AM To: Elizabeth Chlipala; Jan Shivers; histonet Subject: RE: [Histonet] Aperio slide scanner I will echo Liz's comments. I occasionally scan slides with an older Scanscope XT. It is worth the effort to clean and QC slides for artifacts as Liz points out as well as placing the section in the middle of the slide. Actual scanning is quite easy, each initial 'snapshot' is reviewed, AOI is resized to boundaries of the tissue and focus points are checked and verified to be overlaying tissue (not white space). I find that adding more focus points across the section greatly increased the quality of the image and rarely have I found any areas out of focus. Once the snapshot reviews are completed simply hit the 'One Touch' icon and batch scanning begins. I really like the ImageScope viewing program which lets one view the section and multiple magnifications, capture images, etc.. I have another system for IHC quantification, but other users are using the Aperio software to do that if that is part of your plan Brett Brett M. Connolly, Ph.D. Principle Scientist, Imaging Dept. Merck Co., Inc. PO Box 4, WP-44K West Point, PA 19486 brett_conno...@merck.com T- 215-652-2501 F- 215-993-6803 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, June 11, 2014 12:46 PM To: Jan Shivers; histonet Subject: RE: [Histonet] Aperio slide scanner Jan They are not that difficult to operate but you do need person with some histology training scanning and QC'ing the slides and images. The need to understand initially if the section is good enough to scan, we QC our slides prior to placing them on the scanner, ones that have sectioning artifacts may not scan that well. Section placement on the slide is important, you can't have anything to close to the edge of a slide it will not scan well. The person responsible for scanning makes sure that the slide is clean and does not have any excess mounting media prior to placing on the scanner, excess mounting media and dirt may cause the scan to be out of focus, and then once the slides are scanned the scans need to be QC'd to make sure that they are good enough for the pathologist or whatever you are utilizing them for. Quality and consistency in histology preparation is key for good scanning results. These scanners scan in primarily one focal plane, meaning the pathologist loses the ability to fine focus as they would on a microscope. They are able to fine focus through poorer quality sections or uneven sections, scanned images do not have a fine focus unless you scan them at multiple focal planes or z-stack. I have seen some cytology images that have been scanned in a way that you have a fine focus slider but its not common for routine histology preps. We take some time upfront to adjust area of interest and check focal points prior to scanning of the slides. We find this works better than just going with the load and go method. We review the snapshots prior to scanning. I'm not sure what scanner you are getting or what version of the image capture software you will be using we have an Aperio ScanScope XT it has a 120 slide capacity. On occasion a particular slide may not scan well, that’s why we like to review the snapshots. For instance we were working on some amniotic membrane constructs these are a single cell layer thick so they are very thin and sometimes the computer does not pick the sample up as tissue because it so thin and can be lightly stained, so we need to place all of the focal points on the slides. You may not have samples like this but you might. Good Luck - feel free to contact me if you need any help once you get the scanner in house. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers Sent: Wednesday, June 11, 2014 10:25 AM To: histonet Subject: [Histonet] Aperio slide scanner My
[Histonet] Dako
Any feedback out there on the Dako Omnis? Deloris Carter HT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] non-fluorescent tissue marking dyes
I am working on a pathology study involving two photon microscopy of pathology samples. For our study we need to be able to mark the surface aspects of our tissue with dye prior to histology processing and multiphoton imaging. Unfortunately, most of the standard tissue marking dyes we use have extraordinarily strong fluorescence in the blue/green when excited in the near UV (2 photon wavelength). This strong fluorescence then triggers the overcurrent protection on our detectors if we stray onto the surface aspect. Are there any tissue marking dyes that are known to survive histology processing and also generate little or no blue/green fluorescence? I'm not concerned about yellow/red fluorescence (its filtered out), or attenuation without fluorescence as we don't generally image the ink directly, it just sometimes gets in the way. I've ordered a few dyes for testing based on their composition, but I'm essentially just guessing because I haven't been able to find much information. I suppose not many people need to fluorescent image inked specimens. Thanks, Mike ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
