[Histonet] THICK AND THIN SECTIONS ?
If you are using one of the following Microtomes and the advance mechanism is worn out. (too much play) REICHERT/JUNG 2030 LEICA RM 2125 LEICA 2030 Biocut LEICA/JUNG 2035 LEICA - CM1850 Cryostat SAKURA SRM 200 You could be faced with purchasing a new Microtome, because these Models are no longer supported by the Manufacturer. ( no parts availability ) Rather than replacing these excellent instruments, I have a PERMANENT solution to this problem. For Information contact: Klaus Dern Phone: 706 635-8840 E-Mail: klaus.der...@gmail.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC for plague and tularemia
Hi everyone, I would love to hear from anyone who is running plague and/or tularemia IHC. If you have a protocol you are willing to share and/or vendor information it would be greatly appreciated. Thank you in advance! Carole Johnson, HT(ASCP)cm Histopathology Section Supervisor New Mexico Department of Agriculture Veterinary Diagnostic Services Confidentiality Notice: New Mexico has a very broad public records law. Most written communications to or from state employees are public records. Your e-mail communications may therefore be subject to public disclosure. This e-mail, including all attachments is for the sole use of the intended recipients. Any unauthorized review, use, disclosure or distribution is prohibited unless specifically provided under the New Mexico Inspection of Public Records Act. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Can I use no water benzoyl peroxide from PolySciences in my MMA mixture?
I try to avoid CaCl drying step in making MMA infiltration and embedding solutions. Please help me MMA experts. Many lab use benzoyl peroxide which has 30% water in it. And go through CaCl afterwards. Another question, why some lab use Butanol and toluene before coverslip? Why use Cellosolve to do deplastify? I think xylene and ethanol are cheaper and less toxic, is it true? Thanks for you reply. Dorothy MGH endocrine histocore Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: IHC Validation
Thank you to everyone who sent a template. This is very helpful. I do need clarfication on the 10 cases that are required for validation. Do you include the 10 cases on this same form, or do you have something separate for that? These look ideal for optimization. From what I have read online, 10 cases need to be run after optimization has been completed. When validating these 10 cases, accuracy, sensitivity, specificity and reproducibility need to be monitored. Are all of you using separate form to capture this data and approval from the Pathologists? Maybe I am going overboard, but I want to make sure that I have everything covered. We are bringing a new platform into our lab in 2 weeks, and I want to have everything ready to go. Leslie Graves Technical Specialist Cape Fear Valley Medical Center Fayetteville, NC 28341 910-615-6142 lg...@capefearvalley.com On Mon, Jul 14, 2014 at 1:55 PM, Leslie Graves lsgrave...@gmail.com wrote: Does anyone have a sample IHC Validation worksheet that they used in their lab for review. I know that the requirements have changed since our last inspection. I want to make sure that I am including all of the criteria, and would like to see how other labs are documenting this process. Leslie Graves Technical Specialist Cape Fear Valley Medical Center 1638 Owen Drive Fayetteville, NC 28341 910-615-6142 lg...@capefearvalley.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC Validation
Can you forward any templates to me as well? Thanks! -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Leslie Graves Sent: Tuesday, July 15, 2014 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: IHC Validation Thank you to everyone who sent a template. This is very helpful. I do need clarfication on the 10 cases that are required for validation. Do you include the 10 cases on this same form, or do you have something separate for that? These look ideal for optimization. From what I have read online, 10 cases need to be run after optimization has been completed. When validating these 10 cases, accuracy, sensitivity, specificity and reproducibility need to be monitored. Are all of you using separate form to capture this data and approval from the Pathologists? Maybe I am going overboard, but I want to make sure that I have everything covered. We are bringing a new platform into our lab in 2 weeks, and I want to have everything ready to go. Leslie Graves Technical Specialist Cape Fear Valley Medical Center Fayetteville, NC 28341 910-615-6142 lg...@capefearvalley.com On Mon, Jul 14, 2014 at 1:55 PM, Leslie Graves lsgrave...@gmail.com wrote: Does anyone have a sample IHC Validation worksheet that they used in their lab for review. I know that the requirements have changed since our last inspection. I want to make sure that I am including all of the criteria, and would like to see how other labs are documenting this process. Leslie Graves Technical Specialist Cape Fear Valley Medical Center 1638 Owen Drive Fayetteville, NC 28341 910-615-6142 lg...@capefearvalley.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Re: IHC Validation
I document all of the worksheet process¹ and then I create a ³Validation Report² where my technical director both reviews all slides and signs this report. Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 7/15/14, 7:51 AM, Leslie Graves lsgrave...@gmail.com wrote: Thank you to everyone who sent a template. This is very helpful. I do need clarfication on the 10 cases that are required for validation. Do you include the 10 cases on this same form, or do you have something separate for that? These look ideal for optimization. From what I have read online, 10 cases need to be run after optimization has been completed. When validating these 10 cases, accuracy, sensitivity, specificity and reproducibility need to be monitored. Are all of you using separate form to capture this data and approval from the Pathologists? Maybe I am going overboard, but I want to make sure that I have everything covered. We are bringing a new platform into our lab in 2 weeks, and I want to have everything ready to go. Leslie Graves Technical Specialist Cape Fear Valley Medical Center Fayetteville, NC 28341 910-615-6142 lg...@capefearvalley.com On Mon, Jul 14, 2014 at 1:55 PM, Leslie Graves lsgrave...@gmail.com wrote: Does anyone have a sample IHC Validation worksheet that they used in their lab for review. I know that the requirements have changed since our last inspection. I want to make sure that I am including all of the criteria, and would like to see how other labs are documenting this process. Leslie Graves Technical Specialist Cape Fear Valley Medical Center 1638 Owen Drive Fayetteville, NC 28341 910-615-6142 lg...@capefearvalley.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Image uploading
Hi Carl, I have sent you a username and password to be able to edit your image posts. Let me know if you have any problems or suggestions. You have definitely posted some great looking images. Best Regards, Marvin Hanna On 07/12/2014 03:17 PM, Hobbs, Carl wrote: I make mistakes when I upload images So, I'd be most grateful for an edit function to be included. Thanks for the opportunity to share images. Sure, the excellent Immunoportal was The Best...I hope that Histonet can equal that website quality? Carlos Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] doing histology in an academic research setting
Hi everyone, I have been doing histology professionally for the past 5 years in an academic research lab setting (and an additional 5 years for my own grad studies). I take pride in the samples I have processed, cut, stained, and I love being able to troubleshoot any challenges or questions that the lab encounters. While I have this feeling for the actual work churned out, I can’t help but feel left out in lab meetings when actual data are presented and discussed. It makes me feel I don’t do enough. I guess what I’ve struggled with is that I do want to be a part of doing data procurement and analysis, but with the amount of work routine histology requires there’s not enough time in the day to also do microscope work and count for data. For those who are in a similar setting, do you share the same feeling? My apologies in advance if this is a silly thing to share/ask, but it has been bugging me for some time now. Thanks, Cecilia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Antibodies to fungus
I think I asked this question a few years ago, but I'm going to ask again DAKO used to sell monoclonal antibodies to Aspergillus (clone Mab-WF-AF-1) and Rhizomucor (clone Mab-WSSA-RA-1). Unfortunately, they discontinued these reagents several years ago. Does anyone know if these antibodies are still available through another vendor? Thank you. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 Office (860) 545-2204 Fax richard.car...@hhchealth.orgmailto:richard.car...@hhchealth.org This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Microtome calibration
Hi Everyone, During a recent inspection we were asked by the inspection team to start calibrating our microtomes. Anyone know of a service vendor that will do this? Referrals, including self-referrals would be greatly appreciated. Thank, Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Control block validation requirements
Same inspectors advised our special stain and IHC controls should have at least two if not three different validation means (PCR, micro, serology, etc.) Anyone out there actually doing this? Just so you know, I've spoken with a few suppliers on control slides and they do not do this type of validation on their control material (surprise, surprise!) Thanks, Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] ER+/PR- in elderly patients
I was wondering if anyone is seeing a trend in ER+/PR- tumors more frequent in older patients? If so, do you have any information regarding this? Thanks~ Brandy Burnett, HTL ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome calibration
Hi Denise, Check with the company that does your PM service. This may be something that is already done during preventative maintenance. On Jul 15, 2014, at 3:02 PM, Long, Denise denise.l...@uconn.edu wrote: Hi Everyone, During a recent inspection we were asked by the inspection team to start calibrating our microtomes. Anyone know of a service vendor that will do this? Referrals, including self-referrals would be greatly appreciated. Thank, Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Microtome calibration
We do ours by hand? You mean line them up or PM? Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com On 7/15/14, 1:55 PM, Ronda Mire rm...@cvpath.org wrote: Hi Denise, Check with the company that does your PM service. This may be something that is already done during preventative maintenance. On Jul 15, 2014, at 3:02 PM, Long, Denise denise.l...@uconn.edu wrote: Hi Everyone, During a recent inspection we were asked by the inspection team to start calibrating our microtomes. Anyone know of a service vendor that will do this? Referrals, including self-referrals would be greatly appreciated. Thank, Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Control block validation requirements
Denise, When selecting infectious agent positive controls, I also select cases which have been shown to be positive by other testing means (EM, Bacteriology, Virology, PCR). In addition, I run a cross-reactivity validation with my antibody on other infectious agent positive control slides to make sure my antibody is specific for the infectious agent and doesn't cross-react with other viruses/bacteria, etc. Results go into a table that is stored along with the initial antibody validation/optimization logsheet(s). When validating tissue antigens/tumor markers, I use the obvious normal anatomic cell/tissue control for that antigen. My anatomic pathologist advisor signs off on the final optimization for all antibodies before the IHC test can be put into public use. Jan Shivers Senior Scientist IHC/Histology Section Head Pathology Teaching Program Veterinary Diagnostic Laboratory University of Minnesota 1333 Gortner Ave. St. Paul, MN 55108 612-624-7297 shive...@umn.edu On Tue, Jul 15, 2014 at 2:05 PM, Long, Denise denise.l...@uconn.edu wrote: Same inspectors advised our special stain and IHC controls should have at least two if not three different validation means (PCR, micro, serology, etc.) Anyone out there actually doing this? Just so you know, I've spoken with a few suppliers on control slides and they do not do this type of validation on their control material (surprise, surprise!) Thanks, Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Microtome calibration
Could they have meant an aligner - something like this? http://www.newcomersupply.com/products/microtome-cryostat-aligners?page=M#1130 Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Long, Denise Sent: Tuesday, July 15, 2014 3:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microtome calibration Hi Everyone, During a recent inspection we were asked by the inspection team to start calibrating our microtomes. Anyone know of a service vendor that will do this? Referrals, including self-referrals would be greatly appreciated. Thank, Denise M. Long, MS, HTL (ASCP), QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences Connecticut Veterinary Medical Diagnostic Laboratory 61 N. Eagleville Road, Unit 3089 Storrs, Connecticut 06269-3089 (860) 486-0851 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Temp full time job in SW WA
We have a temporary full time position to fill. We are looking for a ASCP certified Histotech that can work from the beginning of September to the end of December/beginning of January. This would be full time coverage for a maternity leave. Hours 6-2:30. Qualifications: ASCP certified Histotech Some Histology work experience (preferred) Responsibilities: Embed Microtomy Manual Special Stains Immunohistochemistry Other duties as assigned Thank you! Suzanne Please respond to aa...@lcpath.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
