RE: [Histonet] Validation of IHC
Dear Jamal, Please see Principles of Analytic Validation of Immunohistochemistry Assays Published : Archives of Pathology and Laboratory Medicine March 19, 2014 cap.org Later on I will send you template of IHC Validation, which we are using in SKMCHRC. Regards Muhammad Tahseen Senior Supervisor Histopathology SKMCHRC Lahore, Pakistan -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jamal Sent: Saturday, July 19, 2014 3:42 PM To: Histonet edu Subject: [Histonet] Validation of IHC Dear colleagues We purchased Ventana GX from Rosh for IHC I reviewed the resent CAP check list 2013 for validation of IHC but I got confused. I am using ready to use prediluted antibodies from Ventana and I want to validate the antibodies before use according to CAP requirements. Can I have the validation plane and procedure for IHC antibodies. Best Regards, Jamal M. Al Rowaihi Anatomic Pathology Supervisor | Al Borg Medical Laboratories | Mobile +966 503629832| mailto:j.rowa...@alborglaboratories.com j.rowa...@alborglaboratories.com Palestine St, Al Rajhi Building, P.O. Box 52817, Jeddah 21573, KSA| Phone: +966 12 670 0099 | Fax: +966 12 676 4984 | http://www.alborglaboratories.com/ www.alborglaboratories.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Clinical EM Labs?
I definitely have to agree about the dying breed. Our hospital network closed down our whole specialty lab which included EM. It was very sad day when I had to turn off the scope for the last time. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Saturday, July 19, 2014 9:37 AM To: Paula Sicurello; HistoNet; microsc...@microscopy.com Subject: Re: [Histonet] Clinical EM Labs? I have some experiences in asking such type of general questions to Histonet members and frequently the answers are few and far apart. I think that you should prepare a very short questionnaire and give it out at the next NSH meeting. Perhaps the NSH could have some idea or the National Health Institute or the National Academy of Sciences. You are right when writing that EM labs are a dying breed. We used to be and no longer are. René J. On Friday, July 18, 2014 4:11 PM, Paula Sicurello pat...@gmail.com wrote: Hello Netters and Listers, I was wondering how many clinical EM labs there are in the US? I get the feeling that we are becoming a small group and just wanted to see who else is out there. My lab has two services: surgical pathology and diagnostic virology. Please contact me if you are clinical EM as well. I figured, if we are a small group, we should get to know each other. Thanks and have a nice weekend. Sincerely, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] FT DERM POSITION DELRAY BEACH, FLORIDA
Hi Histonetters! We are looking for a full time licensed histotech here in our very busy Delray Florida Derm Lab. This is a permanent full time position with benefits (medical/401k/vacation). DRUG FREE WORKPLACE. ***PLEASE NO HEAD HUNTERS/PLACEMENT SERVICES***!!! Email your resume to lengim...@leavittmgt.commailto:lengim...@leavittmgt.com if interested. *full time position Mon-Fri 11a-7:30p (hours NON NEGOTIABLE) *MUST be licensed as a FL histotechnologist or technician *MUST have at least 2 years experience (dermatology preferred) *VERY proficient in embedding and microtomy *experience in grossing and immunohistochemistry (BOND) a plus *must be self motivated and a team player *knowledge in operating Ventana and Leica equipment desired (not necessary) Kari M Simeone ksime...@leavittmgt.commailto:ksime...@leavittmgt.com The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you have received this message in error, you are prohibited from copying, distributing or using the information. Please contact the sender immediately by return e-mail and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Clinical EM Labs?
We closed our EM laboratory many years ago after our Director passed away unexpectedly and we could not find anyone to take his place. We now send all of our medical and transplant kidney biopsies to Columbia in NYC for diagnosis including EM. Other specimens (e.g., ciliary biopsies, heart biopsies for amyloid, an occasional liver biopsy, etc.) are sent to Yale. Although we run a large Anatomic Pathology operation there are very few specimens that require EM. We no longer use EM for tumors and most infectious disease cases are resolved with IHC. It's unfortunate (given our size) that we can no longer provide these services on-site; however, I'm a firm believer of Centers of Excellence and I think sending these specimens out to centers that are performing EM on a daily basis is best for the patients that we serve. Richard Cartun From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Paula Sicurello [pat...@gmail.com] Sent: Friday, July 18, 2014 4:11 PM To: HistoNet; microsc...@microscopy.com Subject: [Histonet] Clinical EM Labs? Hello Netters and Listers, I was wondering how many clinical EM labs there are in the US? I get the feeling that we are becoming a small group and just wanted to see who else is out there. My lab has two services: surgical pathology and diagnostic virology. Please contact me if you are clinical EM as well. I figured, if we are a small group, we should get to know each other. Thanks and have a nice weekend. Sincerely, Paula -- Paula Sicurello, HTL (ASCP) Supervisor, Clinical Electron Microscopy Laboratory Duke University Health System Rm.#251M, Duke South, Green Zone Durham, North Carolina 27710 P: 919.684.2091 HIPAA Privacy Notification: This message and any accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. 2510-2521, and contain information intended for the specific individual (s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying or the taking of any action based on the contents of this information is strictly prohibited . If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HS IHC project
Friends, I am going to help a local HS class (a Biotech class) do a simple IHC project. This class already processes tissue, embeds, sections and does an HE stain. My husband fixed up an old black AO microtome for them to use and they actually cut sections. They cannot use human tissue there so what they have available is mouse spleen already ffpe. I was thinking of using a rab antibody that works on ffpe ms spleen. Ki67 comes to mind? It has been a while since I was in the lab so I am consulting with you all to make sure a rab poly or rab mono Ki67 will indeed stain a ffpe ms spleen? If you have evidence of this can you tell me which ab was used successfully. If you can think of a better rab ab to use on the ffpe ms spleen for this project let me know. It would help if we could keep it simple for antigen retrieval. One of our kind vendors will be donating IHC reagents for this HS class project. I hope the IHC meeting is going well in Vegas, wish I was there. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HS IHC project
T cell and B cell markers work well with human spleen. From: Patsy Ruegg pru...@ihctech.net To: Histonet@Lists. Edu histonet@lists.utsouthwestern.edu Date: 07/21/2014 11:08 AM Subject:[Histonet] HS IHC project Sent by:histonet-boun...@lists.utsouthwestern.edu Friends, I am going to help a local HS class (a Biotech class) do a simple IHC project. This class already processes tissue, embeds, sections and does an HE stain. My husband fixed up an old black AO microtome for them to use and they actually cut sections. They cannot use human tissue there so what they have available is mouse spleen already ffpe. I was thinking of using a rab antibody that works on ffpe ms spleen. Ki67 comes to mind? It has been a while since I was in the lab so I am consulting with you all to make sure a rab poly or rab mono Ki67 will indeed stain a ffpe ms spleen? If you have evidence of this can you tell me which ab was used successfully. If you can think of a better rab ab to use on the ffpe ms spleen for this project let me know. It would help if we could keep it simple for antigen retrieval. One of our kind vendors will be donating IHC reagents for this HS class project. I hope the IHC meeting is going well in Vegas, wish I was there. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HS IHC project
I am so jealous of these kids. I didn't get near a microscope until college, let alone a microtome. I hope they have fun! Emily By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward. -Chuck Palahniuk, Haunted On Mon, Jul 21, 2014 at 2:19 PM, Jennifer MacDonald jmacdon...@mtsac.edu wrote: T cell and B cell markers work well with human spleen. From: Patsy Ruegg pru...@ihctech.net To: Histonet@Lists. Edu histonet@lists.utsouthwestern.edu Date: 07/21/2014 11:08 AM Subject:[Histonet] HS IHC project Sent by:histonet-boun...@lists.utsouthwestern.edu Friends, I am going to help a local HS class (a Biotech class) do a simple IHC project. This class already processes tissue, embeds, sections and does an HE stain. My husband fixed up an old black AO microtome for them to use and they actually cut sections. They cannot use human tissue there so what they have available is mouse spleen already ffpe. I was thinking of using a rab antibody that works on ffpe ms spleen. Ki67 comes to mind? It has been a while since I was in the lab so I am consulting with you all to make sure a rab poly or rab mono Ki67 will indeed stain a ffpe ms spleen? If you have evidence of this can you tell me which ab was used successfully. If you can think of a better rab ab to use on the ffpe ms spleen for this project let me know. It would help if we could keep it simple for antigen retrieval. One of our kind vendors will be donating IHC reagents for this HS class project. I hope the IHC meeting is going well in Vegas, wish I was there. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com pru...@ihctech.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
