[Histonet] RE: Snomed
We haven't used snomed codes for many years. Tom Mc Nemar, HT(ASCP) Histology Supervisor (740) 348-4163 Licking Memorial Hospital 1320 West Main Street Newark, OH 43055 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jessica.va...@hcahealthcare.com Sent: Wednesday, November 19, 2014 2:35 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Snomed How many of your pathologists, LIS or others are using Snomed? Jessica Vacca HCA Epic Anatomic Pathology Application Lead Clinical Services Group 2545 Park Plaza Bldg III Nashville, TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Flames at embedding centers
Darn right! We'll live forever at this rate of fixation... Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Wednesday, November 19, 2014 2:46 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Old Histotechs never die. They're just well fixed... :). From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Mark Turner [mtur...@csilaboratories.com] Sent: Wednesday, November 19, 2014 1:53 PM To: Sanders, Jeanine (CDC/OID/NCEZID); Blazek, Linda; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers We had a special clean counter we used for pizza on a regular basis. I worked with a pathologist who refused to wear gloves and would gross colons bare-handed. Guy is still alive and kicking at 85! In the very old days, we used carbon tetrachloride to dehydrate in the open tissue processor (Technicon). Not going to say anything at all about disposal Mark Turner, Ph.D., HT(ASCP)QIHC Manager, Histology/IHC -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:10 PM To: Blazek, Linda; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Okay, since we are confessing. When I was a student in histology school, we did the potluck thing IN THE LAB! I mean, ALL the food was laid out on a back counter -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Wednesday, November 19, 2014 2:08 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers Since the break room was right across the hall from the histology lab, we use to clear off a counter to put all the food for our potlucks. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders, Jeanine (CDC/OID/NCEZID) Sent: Wednesday, November 19, 2014 2:04 PM To: James Watson; 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers sigh the good old days -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of James Watson Sent: Wednesday, November 19, 2014 1:58 PM To: 'Bob Richmond'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Flames at embedding centers How about the person in the 1970's coverslipping with an open dish of xylene at AFIP and someone at the other end of the stain line decolorizing Brown and Brenn stains with acetone/ether in the sink; then the acetone/ether fumes migrating across the stain line to the cigarette and having the whole counter and wall catch fire. James Watson HT ASCP GNF Genomics Institute of the Novartis Research Foundation Scientific Technical Leader II, Histology Tel858-332-4647 Fax 858-812-1915 jwat...@gnf.org -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: mouse carotid body
Dear Colleagues. Please share your expertise how to reveal the carotid body in mouse carotid artery. I received from researcher the piece of mouse carotid artery with bifurcation on internal and external carotid branches. What is the best way for embedding: flat parallel to the mold bottom or on transverse axis, that is perpendicular to the mold bottom.? I think I should collect many serial sections and maybe in couple of them I will be able to find carotid body. Am I right? Please advise better and rational way to do this.Thank you Galina DeynekoNovartis, Cambridge, MA 617-871-7613 w ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HPV ISH
What do others do for HPV ISH? Do you do the stain yourself in-house, or do you send it out? I know there used to be a stainer specifically for ISH - does this still exist and who is the vendor? I heard there is some kind of conflict between vendors and the FDA?? Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] open histology position
Temporary Histotechnologist Position in St. Paul, MN Interested in making some extra holiday cash? Regions Hospital is looking for an experienced histotechnologist to work a temporary, full time night shift through January 31, 2015. Regions Hospital is a Level I Adult and Pediatric trauma Center and teaching hospital serving Minnesota and western Wisconsin for more than 130 years. Regions is a private, non-profit hospital providing outstanding care in women's health, heart, cancer, surgery, orthopaedics, neurosciences, burn, emergency care, and more. Regions Hospital Lab is a state of the art. Regions is a part of the HealthPartners Family of Care. Additional information is available at regionshospital.com. Regions Hospital employees enjoy opportunities for personal and professional growth available only at one of the top teaching hospitals in the Twin Cities area. Our dedication to patient care and commitment to a healthy workplace, and Best Care, Best Experience, has allowed us to be recognized by the Minnesota Hospital Association with the Best Minnesota Hospital Workplace Award. This position will perform tests and procedures in our histology laboratory; perform functions associated with obtaining, receiving and processing of surgical pathology specimens in patients of all ages, to maintain laboratory equipment and supplies, and to perform related duties as assigned. Qualifications: Histotechnician board certification, ASCP or equivalent certification preferred. Two (2) years of Histology work experience within the last five (5) years may substitute for the educational qualifications. Education: Completion of an approved histology program and clinical histology internship or completion of an accredited two year laboratory technician training program in a vocational school or junior college. How to Apply: Apply online at www.regionshospital.comhttps://careers.peopleclick.com/careerscp/client_healthpartners/regions_external/jobDetails.do?functionName=getJobDetailjobPostId=59124localeCode=en-ushttp://www.regionshospital.com%3chttps:/careers.peopleclick.com/careerscp/client_healthpartners/regions_external/jobDetails.do?functionName=getJobDetailjobPostId=59124localeCode=en-us Additional Information We are an Equal Opportunity Employer and do not discriminate against applicants due to race, ethnicity, gender, veteran status, or on the basis of disability or any other federal, state or local protected class. Dorothy Webb, HT (ASCP) 651-254-2962 This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Flames at embedding centers
Ah, such an appropriate time of year for this thread. Once a year we gave our trash autoclave a good cleaning and pressure cooked turkeys for our Thanksgiving party. Ed Gray | Clinical Application Coordinator | Phone: 304-293-2945 | Fax: 304-293-1627 | WVU Healthcare l eg...@wvuhealthcare.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Wednesday, November 19, 2014 10:36 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Flames at embedding centers Remembering histotechnologists at Johns Hopkins in the 1960s smoking cigarettes while hand-staining slides in rows of large Stender dishes, including a dish with 20% picric acid in acetone, used to remove formalin pigment (since buffering formalin wasn't permitted way back then). Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CD 34 and CD117
Who are your favorite vendors for CD34 (blasts basophils) and CD117 (immature granulocytes) in bone marrow tissues. Currently utilizing the Ventana Benchmark Ultra platform. Interested in finding a different vendor that will make our pathologist happier. Our current antibody is working, but he's just not happy. Many Thanks for All your Input, Susan ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Jores, Klotz and that pesky chloral hydrate
Hi All, I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle). Does anyone have any thoughts about just leaving the chloral hydrate out? Or, an alternative fixative that doesn't have the chloral hydrate? I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two. Thanks a bunch, M -- Molly Murphy DVM, Ph.D Assistant Professor of Veterinary Pathology College of Natural Sciences Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Jores, Klotz and that pesky chloral hydrate
Hi Molly, You might just want to fix in buffered 10% formalin and then rinse specimens well in water ( maybe 20 minutes) before using in class and then put back in formalin. Tom T -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molly Murphy Sent: Thursday, November 20, 2014 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate Hi All, I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle). Does anyone have any thoughts about just leaving the chloral hydrate out? Or, an alternative fixative that doesn't have the chloral hydrate? I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two. Thanks a bunch, M -- Molly Murphy DVM, Ph.D Assistant Professor of Veterinary Pathology College of Natural Sciences Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Jores, Klotz and that pesky chloral hydrate
How about formalin substitute from Anatech - Propar I think it is.. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom Sent: Thursday, November 20, 2014 4:24 PM To: Molly Murphy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Jores, Klotz and that pesky chloral hydrate Hi Molly, You might just want to fix in buffered 10% formalin and then rinse specimens well in water ( maybe 20 minutes) before using in class and then put back in formalin. Tom T -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molly Murphy Sent: Thursday, November 20, 2014 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate Hi All, I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle). Does anyone have any thoughts about just leaving the chloral hydrate out? Or, an alternative fixative that doesn't have the chloral hydrate? I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two. Thanks a bunch, M -- Molly Murphy DVM, Ph.D Assistant Professor of Veterinary Pathology College of Natural Sciences Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Jores, Klotz and that pesky chloral hydrate
We are a veterinary pathology teaching lab and we used Klotz with chloral hydrate to preserve our gross teaching specimens. We switched to a Klotz recipe that does not use chloral hydrate because the chloral hydrate is hard to source and very expensive. It is maybe not quite as good as the Klotz with chloral hydrate but seems to work OK. Here is the recipe. Sodium chloride 208 g Sodium bicarbonate 375 g Sodium sulfate 458 g Formaldehyde, 37% 667 ml Water To make up to 50 L Regards, Betty Pollock Manager, Operations DSU Faculty of Veterinary Medicine University of Calgary Calgary, AB, Canada Tel: 403-220-2806 FAX: 403-239-6984 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molly Murphy Sent: Thursday, November 20, 2014 2:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate Hi All, I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle). Does anyone have any thoughts about just leaving the chloral hydrate out? Or, an alternative fixative that doesn't have the chloral hydrate? I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two. Thanks a bunch, M -- Molly Murphy DVM, Ph.D Assistant Professor of Veterinary Pathology College of Natural Sciences Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Jores, Klotz and that pesky chloral hydrate
Well if the specimens are well fixed in formalin, then bring back the colour with alcohol and you can the store them in a non-formalin solution such as dilute honey or high salt solution: Henwood, A., (2002) Color preservation in pathology museum specimens Biotechnic Histochem 77(4):230. Özkan, N., Salva, E., Cakalagaoglu, F., Tüzüner, B. (2012). Honey as a substitute for formalin?. Biotechnic Histochemistry, 87(2), 148-153. Al-Maaini R, Bryant P (2006) The effectiveness of honey as a substitute for formalin in the histological fixation of tissue. J. Histotechnol. 29: 173–176. Oliveira, F. S. (2014). Assessing the effectiveness of 30% sodium chloride aqueous solution for the preservation of fixed anatomical specimens: a 5‐year follow‐up study. J. Anat. (2014) 225, pp118-121 Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager Senior Scientist, the Children’s Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molly Murphy Sent: Friday, 21 November 2014 8:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Jores, Klotz and that pesky chloral hydrate Hi All, I am looking for a tissue fixative to preserve gross specimens for a veterinary pathology teaching lab (eg. no histo, only gross specimens). I have used Jore's in the past, and Klotz has been recommended, but the chloral hydrate is a problem child due to its status as a controlled substance (eg disposal is a hassle). Does anyone have any thoughts about just leaving the chloral hydrate out? Or, an alternative fixative that doesn't have the chloral hydrate? I have access to a refrigerator for samples, and would *hopefully* keep the specimens for a year or two. Thanks a bunch, M -- Molly Murphy DVM, Ph.D Assistant Professor of Veterinary Pathology College of Natural Sciences Mathematics University of Alaska Fairbanks Office: (907) 474-1990 Fax: (907) 474-1932 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
