Re: [Histonet] (no subject)
Yes, you have to. The origin of the section (MOHS or other) is not the criteria to use controls, but the process is.René J. On Tuesday, December 2, 2014 4:23 PM, Chapman, Cherie J. chapm...@health.missouri.edu wrote: Does anyone know if you have to use controls for IHC on MOHS frozen section procedures to satisfy CAP requirements? Thank you, Cherie Chapman, BS, HT, HTL (ASCP) Associate Director of Dermatopathology Laboratory University of Missouri Department of Dermatology University Physicians Medical Building Phone: (573) 884-0123 Fax: (573) 884-0834 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Water baths
What is everyone using as their histo refrigerator temperature range? Amber Timm, MLS (ASCP)CM Supervisor of Laboratory Technical Services The Diagnostic Treatment Center 3401 Cranberry Blvd. Weston, WI 54476 Phone: 715-393-2485 Email: ti...@dxandtx.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Tuesday, December 02, 2014 10:50 AM To: 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths We still do, due to the fact that it is on our general temperature chart that is used in the department. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.han...@parrishmed.com www.parrishmed.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, December 02, 2014 11:20 AM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths It is no longer a CAP requirement to do this, so we haven't for many years. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet = This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender
[Histonet] RE: Water baths
We use whatever is the narrowest range for the reagents stored in there. So if there is one reagent with a range of 4-10°C, and another with 2-8°C, we'll use 4-8° as our range. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Timm, Amber L Sent: Wednesday, December 03, 2014 11:09 AM To: Hannen, Valerie; 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths What is everyone using as their histo refrigerator temperature range? Amber Timm, MLS (ASCP)CM Supervisor of Laboratory Technical Services The Diagnostic Treatment Center 3401 Cranberry Blvd. Weston, WI 54476 Phone: 715-393-2485 Email: ti...@dxandtx.commailto:ti...@dxandtx.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Tuesday, December 02, 2014 10:50 AM To: 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths We still do, due to the fact that it is on our general temperature chart that is used in the department. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.han...@parrishmed.commailto:valerie.han...@parrishmed.com www.parrishmed.comhttp://www.parrishmed.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, December 02, 2014 11:20 AM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths It is no longer a CAP requirement to do this, so we haven't for many years. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.orgmailto:joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.orghttp://www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.netmailto:tanyaabb...@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet = This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is
[Histonet] Non-GYN Prep Safety
Question for you all Do you process Non-GYN specimens under a hood? What type of precautions are taken for suspect mycobacterium specimens? Are your ThinPrep machines under a hood as well? Thanks in advance Amanda Reichard, HTL (ASCP)cm Histotechnologist Laboratory Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (740) 348-4157 This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Water baths
Thank you all I was looking to verify the tissue storage temp. that we have. Amber Timm, MLS (ASCP)CM Supervisor of Laboratory Technical Services The Diagnostic Treatment Center 3401 Cranberry Blvd. Weston, WI 54476 Phone: 715-393-2485 Email: ti...@dxandtx.com -Original Message- From: Goins, Tresa [mailto:tgo...@mt.gov] Sent: Wednesday, December 03, 2014 10:25 AM To: Timm, Amber L; Hannen, Valerie; 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: RE: Water baths We use the range 2C to 8C - the range published for products requiring refrigeration in the Sigma catalog. tg -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Timm, Amber L Sent: Wednesday, December 03, 2014 9:09 AM To: Hannen, Valerie; 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths What is everyone using as their histo refrigerator temperature range? Amber Timm, MLS (ASCP)CM Supervisor of Laboratory Technical Services The Diagnostic Treatment Center 3401 Cranberry Blvd. Weston, WI 54476 Phone: 715-393-2485 Email: ti...@dxandtx.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Tuesday, December 02, 2014 10:50 AM To: 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths We still do, due to the fact that it is on our general temperature chart that is used in the department. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.han...@parrishmed.com www.parrishmed.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, December 02, 2014 11:20 AM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths It is no longer a CAP requirement to do this, so we haven't for many years. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet = This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee
[Histonet] RE: Water baths
We use the range 2C to 8C - the range published for products requiring refrigeration in the Sigma catalog. tg -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Timm, Amber L Sent: Wednesday, December 03, 2014 9:09 AM To: Hannen, Valerie; 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths What is everyone using as their histo refrigerator temperature range? Amber Timm, MLS (ASCP)CM Supervisor of Laboratory Technical Services The Diagnostic Treatment Center 3401 Cranberry Blvd. Weston, WI 54476 Phone: 715-393-2485 Email: ti...@dxandtx.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, Valerie Sent: Tuesday, December 02, 2014 10:50 AM To: 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths We still do, due to the fact that it is on our general temperature chart that is used in the department. Valerie Hannen,MLT(ASCP),HTL,SU (FL) Section Chief, Histology Parrish Medical Center 951 N. Washington Ave. Titusville,Florida 32796 T: (321)268-6333 ext. 7506 F: (321) 268-6149 valerie.han...@parrishmed.com www.parrishmed.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K. Sent: Tuesday, December 02, 2014 11:20 AM To: 'Abbott, Tanya'; histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Water baths It is no longer a CAP requirement to do this, so we haven't for many years. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya Sent: Tuesday, December 02, 2014 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Water baths Does anyone take daily temperature checks of their water bath (used for cutting only) for CAP? Tanya G. Abbott RT (CSMLS) Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph 610-378-2635 fax 610-898-5871 email: tanyaabb...@catholichealth.net This email and attachments contain information that may be confidential or privileged. If you are not the intended recipient, notify the sender at once and delete this message completely from your information system. Further use, disclosure, or copying of information contained in this email is not authorized, and any such action should not be construed as a waiver of privilege or other confidentiality protections. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet = This email is intended solely for the use of the individual to whom it is addressed and may contain information that is privileged, confidential or otherwise exempt from disclosure under applicable law. If the reader of this email is not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this communication in error, please immediately delete this message. Thank you = ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail
[Histonet] Prostate Needle Bx
How do others process prostate needle bx's - what kind of cassettes do you use? Do you use sponges? Do you wrap the tissue in lens paper? Do you process them on a biopsy (short) run? Our bx's always seem to be less than optimal, but we do not have problems with other small bx's. Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Prostate Needle Bx
I have discovered a wonderful cassette from Cancer Diagnostics, it is called the Autocassette and has an oval concave cavity with no corners for small pieces of tissue to get caught in and the solutions pass through it very nicely. I use it for small research samples, like shrimp gills similar in size to the prostate biopsies, and I lose nothing. I use the yellow ones, VB1005 but they have other colors and also some with smaller grid. Highly recommend them. Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, December 03, 2014 11:49 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Prostate Needle Bx How do others process prostate needle bx's - what kind of cassettes do you use? Do you use sponges? Do you wrap the tissue in lens paper? Do you process them on a biopsy (short) run? Our bx's always seem to be less than optimal, but we do not have problems with other small bx's. Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Non-GYN Prep Safety
Specimen prep under a hood - universal precautions - ThinPrep not under a hood. Joyce Weems Pathology Manager 678-843-7376 Phone 678-843-7831 Fax joyce.we...@emoryhealthcare.org www.saintjosephsatlanta.org 5665 Peachtree Dunwoody Road Atlanta, GA 30342 This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amanda Reichard Sent: Wednesday, December 03, 2014 11:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Non-GYN Prep Safety Question for you all Do you process Non-GYN specimens under a hood? What type of precautions are taken for suspect mycobacterium specimens? Are your ThinPrep machines under a hood as well? Thanks in advance Amanda Reichard, HTL (ASCP)cm Histotechnologist Laboratory Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (740) 348-4157 This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail message (including any attachments) is for the sole use of the intended recipient(s) and may contain confidential and privileged information. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this message (including any attachments) is strictly prohibited. If you have received this message in error, please contact the sender by reply e-mail message and destroy all copies of the original message (including attachments). ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MMA Mounting Issue with Haupts Adhesive
Hi all, I had a question about how to properly mount MMA sectioned slides using Haupt's (Dorn and Hart Microedge). My protocol is as follows: 1. Stick Glass Slide into 50mL Falcon Tube with Haupts solution (1:1 50% EtOH:50% Haupts). 2. Rotate the tube to ensure entire coating of glass slide. 3. Let glass slide dry in fume hood for about 10minutes. 4. Remove cut microtome sections (range: 50-5microns). 5. Place cut sections in 70%EtOH to soften the plastic and unroll. (sections are coming out rolled up like scrolls) 6. Remove a section from the 70%EtOH using forceps and try unrolling it with a needle on a glass slide. 7. Once the section(s) is stretched on the coated glass slide, cover with saran wrap. 8. Cover the wrapped slide with coarse filter paper and use a small seam roller to roll the sections on the glass slide as flat as possible. This will also help remove excess 70%EtOH. 9. Remove the filter paper and stack glass slides in a slide press or similar device. Place tongue depressors or small pieces of wood equivalent to the slide length that are about 1cm thick at the end of the glass slide stack. 10. Let slides dry over night at room temperature in press. 11. Remove the slides from the press and unwrap the saran wrap to expose your adhered sample. Questions/Comments: 1. When I pull off the saran wrap, the sample sometimes comes off. The plastic in the sample turns white and dries out and does not attach. This is seen in the majority of my tissue sections. 2. The only time I have noticed consistent attachment is for a 5micron sized section. 3. How can I adjust the microtome so that the samples do not come off so scroll-like? My cutting angle is typically either 0 or 2.5 degrees. I also apply 70% EtOH to the block surface to soften the plastic. Overall goal: Get the tissue sections adhered to the glass slides so that I can proceed with staining. Thank you all. Regards, Jesse Hernandez ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Non-GYN Prep Safety
Unfixed specimen should be done under a biological safety cabinet. Tom -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amanda Reichard Sent: Wednesday, December 03, 2014 11:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Non-GYN Prep Safety Question for you all Do you process Non-GYN specimens under a hood? What type of precautions are taken for suspect mycobacterium specimens? Are your ThinPrep machines under a hood as well? Thanks in advance Amanda Reichard, HTL (ASCP)cm Histotechnologist Laboratory Licking Memorial Health Systems 1320 W. Main St. Newark, OH 43055 (740) 348-4157 This e-mail, including attachments, is intended for the sole use of the individual and/or entity to whom it is addressed, and contains information from Licking Memorial Health Systems which is confidential or privileged. If you are not the intended recipient, nor authorized to receive for the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this e-mail and attachments is prohibited. If you have received this in error, please advise the sender by reply e-mail and delete the message immediately. You may also contact the LMH Process Improvement Center at 740-348-4641. E-mail transmissions cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. The sender therefore does not accept liability for any errors or omissions in the contents of this message, which arise as a result of e-mail transmission. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: FoxP3 and Geminin Immunohistochemistry
Hi Steven, I can't speak to geminin but I have use the following 2 clones of FoxP3 with success (in brightfield) Spring Biosciences clone SP97 (rbMAb) Ebioscience Clone ebio-7979 (msMAb) For both I use the Diva decloaking solution from Biocare so pretty much standard citrate antigen retrieval should get you there. I'm assuming you're talking about human tissue, otherwise I have another one I use on mouse tissue. Katy Milne Deeley Research Centre -Original Message- Message: 2 Date: Tue, 2 Dec 2014 19:37:51 + From: Swartwood, Steven J steven.swartw...@cshs.org Subject: [Histonet] FoxP3 and Geminin Immunohistochemistry To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 959202ac61aef942968646ec66e2be3e3bf09...@espwmsgmbx07.csmc.edu Content-Type: text/plain; charset=us-ascii Hello all, I've done some research on Foxp3 and Geminin and I've seen a few antibodies that look sufficient for IHC in FFPE tissues. My lab is currently looking into purchasing these antibodies for a few different projects. Does anyone on here use these two antibodies for IHC in FFPE tissues? I'm going to use them in bright field, but we may also turn to IF for other studies. I'd be grateful for any information from what clone/company do you buy from and retrieval methods to primary antibody incubations/dilutions. Thank you all for any information, Steven Swartwood HT(ASCP) Cedars Sinai Medical Center steven.swartw...@cshs.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Prostate Needle Bx
I concur with Shirley~ this will be my next order for my GI lab. Wanda ---Original Message--- From: Shirley A. Powell powell...@mercer.edu To: Laurie Colbert lcolb...@pathmdlabs.com, Histonet Post (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Prostate Needle Bx Sent: Dec 03 '14 12:00 I have discovered a wonderful cassette from Cancer Diagnostics, it is called the Autocassette and has an oval concave cavity with no corners for small pieces of tissue to get caught in and the solutions pass through it very nicely. I use it for small research samples, like shrimp gills similar in size to the prostate biopsies, and I lose nothing. I use the yellow ones, VB1005 but they have other colors and also some with smaller grid. Highly recommend them. Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, December 03, 2014 11:49 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Prostate Needle Bx How do others process prostate needle bx's - what kind of cassettes do you use? Do you use sponges? Do you wrap the tissue in lens paper? Do you process them on a biopsy (short) run? Our bx's always seem to be less than optimal, but we do not have problems with other small bx's. Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] FoxP3 and Geminin Immunohistochemistry
Hello Steven, I have used both of these antibodies for various projects and we performed all of these on the Bondmax from Leica with a 60 minute antibody incubation. We have used two FoxP3 antibodies from eBiosciences; cat# 14-4777 on human tissue and cat# 13-5773 for mouse tissue. (both were 1:100 dilutions and ER2 for 20 mins (EDTA pH9). I have only used Geminin on human tissue. It was from Proteintech Group cat# 10802-1-AP (1:1500 dilution using ER1 for 15 min-ER1=citrate pH6) All of these were on FFPE tissues for brightfield only. We did not use them for IF. If you need any other specifics, let me know. Ashley Troutman BS, MBA, HT(ASCP)QIHC Supervisor-Translational Pathology Shared Resource Vanderbilt University Medical Center S-1310 Medical Center North 1161 21st Avenue South Nashville, TN 37232 Message: 2 Date: Tue, 2 Dec 2014 19:37:51 + From: Swartwood, Steven J steven.swartw...@cshs.orgmailto:steven.swartw...@cshs.org Subject: [Histonet] FoxP3 and Geminin Immunohistochemistry To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Message-ID: 959202ac61aef942968646ec66e2be3e3bf09...@espwmsgmbx07.csmc.edumailto:959202ac61aef942968646ec66e2be3e3bf09...@espwmsgmbx07.csmc.edu Content-Type: text/plain; charset=us-ascii Hello all, I've done some research on Foxp3 and Geminin and I've seen a few antibodies that look sufficient for IHC in FFPE tissues. My lab is currently looking into purchasing these antibodies for a few different projects. Does anyone on here use these two antibodies for IHC in FFPE tissues? I'm going to use them in bright field, but we may also turn to IF for other studies. I'd be grateful for any information from what clone/company do you buy from and retrieval methods to primary antibody incubations/dilutions. Thank you all for any information, Steven Swartwood HT(ASCP) Cedars Sinai Medical Center steven.swartw...@cshs.orgmailto:steven.swartw...@cshs.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] FoxP3 and Geminin Immunohistochemistry
I have no xp re Geminin but, I have posted images of FoxP3 in FFPWS on the Histonet Images website. The two abs work well for me. I used a homemade std Citric acid HIER solution. Looks like you have choices, given Katy Milne's response! Good luck. carl ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: MMA Mounting Issue with Haupts Adhesive
Jesse, I have discovered that there are many different ways to work with MMA. Probably no two techs are doing it exactly the same because there are so many steps in which to optimize it for your lab. From reading your protocol, I see similarities and differences. Rather than saran wrap, I use a thicker plastic that I cut into strips ( the size of the slide) from the plastic bags that we use for discarding paraffin waste. Although this is different from your protocol, I still think that what I do to remove the plastic might work for you: just before removing the plastic strip, I shoot it with a blast of freeze spray. The plastic just about falls off. Nancy Thomas Stowers Institute for Medical Research Kansas City, MO -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hernandez, Jesus Willibaldo Sent: Wednesday, December 03, 2014 11:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MMA Mounting Issue with Haupts Adhesive Hi all, I had a question about how to properly mount MMA sectioned slides using Haupt's (Dorn and Hart Microedge). My protocol is as follows: 1. Stick Glass Slide into 50mL Falcon Tube with Haupts solution (1:1 50% EtOH:50% Haupts). 2. Rotate the tube to ensure entire coating of glass slide. 3. Let glass slide dry in fume hood for about 10minutes. 4. Remove cut microtome sections (range: 50-5microns). 5. Place cut sections in 70%EtOH to soften the plastic and unroll. (sections are coming out rolled up like scrolls) 6. Remove a section from the 70%EtOH using forceps and try unrolling it with a needle on a glass slide. 7. Once the section(s) is stretched on the coated glass slide, cover with saran wrap. 8. Cover the wrapped slide with coarse filter paper and use a small seam roller to roll the sections on the glass slide as flat as possible. This will also help remove excess 70%EtOH. 9. Remove the filter paper and stack glass slides in a slide press or similar device. Place tongue depressors or small pieces of wood equivalent to the slide length that are about 1cm thick at the end of the glass slide stack. 10. Let slides dry over night at room temperature in press. 11. Remove the slides from the press and unwrap the saran wrap to expose your adhered sample. Questions/Comments: 1. When I pull off the saran wrap, the sample sometimes comes off. The plastic in the sample turns white and dries out and does not attach. This is seen in the majority of my tissue sections. 2. The only time I have noticed consistent attachment is for a 5micron sized section. 3. How can I adjust the microtome so that the samples do not come off so scroll-like? My cutting angle is typically either 0 or 2.5 degrees. I also apply 70% EtOH to the block surface to soften the plastic. Overall goal: Get the tissue sections adhered to the glass slides so that I can proceed with staining. Thank you all. Regards, Jesse Hernandez ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] re: Flotation bath temperatures
Dear all, I am changing the language to flotation bath (instead of water bath, since that is entirely different lab equipment). I understand there is no longer a CAP requirement to record temperatures. Is there a GLP (or GxP) requirement to do so? Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: re: Flotation bath temperatures
Teri We do, it's more of a combination use log for the microtome and tissue flotation bath, we record temperature and that we cleaned the unit, used fresh water and also cleaned the microtome. This document also records the yearly calibration of the floatation bath and the yearly PM of the microtome. I do need to add that both the calibration and PM will have additional documentation that is stored in a designated binder. I can send you a copy of the form in a separate e-mail if you would like it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Wednesday, December 03, 2014 12:04 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] re: Flotation bath temperatures Dear all, I am changing the language to flotation bath (instead of water bath, since that is entirely different lab equipment). I understand there is no longer a CAP requirement to record temperatures. Is there a GLP (or GxP) requirement to do so? Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: re: Flotation bath temperatures
Although it is not in the Anatomic CAP checklist, there is a new All Common question that does address this, and which, if I'm understanding it, would include flotation baths. All Common checklist, COM.30750, Phase II Temperatures are checked and recorded each day of use for all temperature-dependent equipment and environments using a calibrated thermometer. Toni -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Wednesday, December 03, 2014 2:19 PM To: Teri Johnson; 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: re: Flotation bath temperatures Teri We do, it's more of a combination use log for the microtome and tissue flotation bath, we record temperature and that we cleaned the unit, used fresh water and also cleaned the microtome. This document also records the yearly calibration of the floatation bath and the yearly PM of the microtome. I do need to add that both the calibration and PM will have additional documentation that is stored in a designated binder. I can send you a copy of the form in a separate e-mail if you would like it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 Boulder, CO 80308 (303) 682-3949 office (303) 682-9060 fax (303) 881-0763 cell l...@premierlab.com www.premierlab.com March 10, 2014 is Histotechnology Professionals Day Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive, Unit E Longmont, CO 80504 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Teri Johnson Sent: Wednesday, December 03, 2014 12:04 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] re: Flotation bath temperatures Dear all, I am changing the language to flotation bath (instead of water bath, since that is entirely different lab equipment). I understand there is no longer a CAP requirement to record temperatures. Is there a GLP (or GxP) requirement to do so? Teri Johnson, HT(ASCP)QIHC Manager Clinical Trial Testing Genoptix, Inc. SAN5, Rm. 2005 760.516.5954 (office) 760.516.6201 (fax) CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HE stainer validation
Hello fellow histonetters, Does anyone have a procedure for validation of a new HE stainer, if you do, can you please share it with me? Thank you Albert Santiago, HT(ASCP) Lab Manager Penncutaneous Pathology Services Dermatopathology Lab 3020 Market ST. Ste 201 Philadelphia, PA 19104 215-662-6539 - Lab 215-662-6759-office albert.santi...@uphs.upenn.edumailto:albert.santi...@uphs.upenn.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Prostate Needle Bx
I have seen these cassettes and while they are nice they are rather expensive and with re-embursemnt down everyone is looking at their budget. Also due to the design and the use of bar codes they do not always fit in the engravers. I still like the idea but with the amount of prostates we do my budget will be hit. I also have no more areas on my engraver to put yet another cassette. STP TJUH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New HE stainer validation
Hello fellow histonetters, Does anyone have a procedure for validation of a new HE stainer, if you do, can you please share it with me? Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Prostate Needle Bx
Currently we use the sponges but have always had problems with getting the cores to be straight and getting a whole core in one tissue section. Plus counting the cores and correlating that at embedding. However, we are testing out a system called SmartBx that is an entire system starting at taking the Bx. It uses an imaging system to guide the bx, then a special bx needle system that places the core in a special cassette on a piece of treated paper - perfectly straight and flat. The cores adhere to the paper and the paper goes through to the final block using special molds at embedding. At grossing the bx/paper is simply placed in a cassette and sent to processing. At embedding the paper w/bx is placed tissue-down in the special mold and pressed down with an associated tamper. The final block has perfectly straight cores at the same level exactly 100um from the block face. There has been a learning curve but overall it makes prostate bx's very easy to handle in grossing and at embedding. the slides have full face cores on every single one. Kind of expensive though, so probably only worthwhile if you do a lot of these. http://www.uc-care.com/products/smartbx Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, December 03, 2014 8:49 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Prostate Needle Bx How do others process prostate needle bx's - what kind of cassettes do you use? Do you use sponges? Do you wrap the tissue in lens paper? Do you process them on a biopsy (short) run? Our bx's always seem to be less than optimal, but we do not have problems with other small bx's. Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] RE: Prostate Needle Bx
My Medical Director published a paper on our procedure. If you would be interested I can ask him to share it with you. Andrea - Original Message - From: wsim...@athensgastro.com To: Shirley A. Powell powell...@mercer.edu, Laurie Colbert lcolb...@pathmdlabs.com, histonet histonet@lists.utsouthwestern.edu Sent: Wednesday, December 3, 2014 12:05:55 PM Subject: Re: [Histonet] RE: Prostate Needle Bx I concur with Shirley~ this will be my next order for my GI lab. Wanda ---Original Message--- From: Shirley A. Powell powell...@mercer.edu To: Laurie Colbert lcolb...@pathmdlabs.com, Histonet Post (histonet@lists.utsouthwestern.edu) histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Prostate Needle Bx Sent: Dec 03 '14 12:00 I have discovered a wonderful cassette from Cancer Diagnostics, it is called the Autocassette and has an oval concave cavity with no corners for small pieces of tissue to get caught in and the solutions pass through it very nicely. I use it for small research samples, like shrimp gills similar in size to the prostate biopsies, and I lose nothing. I use the yellow ones, VB1005 but they have other colors and also some with smaller grid. Highly recommend them. Shirley -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Wednesday, December 03, 2014 11:49 AM To: Histonet Post (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Prostate Needle Bx How do others process prostate needle bx's - what kind of cassettes do you use? Do you use sponges? Do you wrap the tissue in lens paper? Do you process them on a biopsy (short) run? Our bx's always seem to be less than optimal, but we do not have problems with other small bx's. Laurie Colbert ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: Prostate Needle Bx
Laurie Colbert asks about processing prostate needle biopsy specimens. The subject is extensively reviewed in this month's or last month's Archives of Pathology. Your pathologists have copies of this if they're fellows of the College of American Pathologists. Here's the method as presented in that article - I've been doing this for a good many years now. The urologist should submit six containers, three for each side of the prostate, each container containing two or more cores. No more than two cores should be put in one cassette. Use those little blue sponges in the cassettes to keep the cores flat. Measure each core, and record the measurements. Don't dye the cores with eosin (fluorescent) - if you want to dye them use safranin O. Process with other small biopsy specimens. Embed using a tamper to get the cores flat in the bottom of the boat. Cut sections onto slides suitable for immunohistochemistry. Cut 5 sections of each block, with little if any trimming. Stain slides 1, 3, and 5 with H E. Reserve slides 2 4 for possible immunohistochemistry (needed in about a tenth of cases). It's a lot of work, but it makes cancer diagnoses. Expect a cancer diagnosis in about a third of cases. Bob Richmond Samurai Pathologist Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
