[Histonet] Cyberlab pathology manual and Dragon
I have recently moved to a new organization and am setting up a new surgical pathology lab. The lab LIS system is Cyberlab and I am told that they have a AP module. In the past I have worked with Cerner Classic and CoPath Plus at a hospital but this lab will be a much smaller set-up. Obviously the organization would like us to use the AP module since the interfacing issues would be markedly reduced. I am set for a demonstration of the pathology module later this week but wondered if anyone could share their experiences with Cyperlab and specifically their pathology module. We will mainly be handling GI biopsies and skin biopsies. The clinic also has Dragon being used in other areas. One of my big questions is whether we will be able to use voice recognition in grossing and for result reporting by the pathologist. If you can share any information I would be very appreciative. Thanks Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cutting honey bees
for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Celestin Blue B
Hello Histologists: Is there anywhere to get this ? Celestin Blue B C.I. 51050 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HELP! Need some old fashioned histology advice
I don't know if the slides were subbed or not, but they looked improvised. The point is, at some point the sections are going to have to be mounted for visualisation, right? Someone or something is going to look at the section under magnification? If you mount them on glass *before* staining, the whole problem of batching becomes one of improvising a giant stain rack and giant staining line. Don't forget, you need giant cover slips, giant slide folders, and giant postdocs. I know that lab glassware is custom ordered every day. Sounds expensive and time consuming to set up, but it beats fiddling around with free floating tissue anyday, IMHO. I don't know what the Ab penetration is going to be like on a 100um section, because they weren't using IHC at Genentech, they were doing in vitro DNA hybridization on the slides. I'm assuming the section thickness is necessary because the PI wants to follow axonal pathways and see the patterns of staining? Sounds like a fun project. If you need more help later you can PM me. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Mon, Jan 5, 2015 at 10:38 PM, Mejia, Mary mary.me...@ucsf.edu wrote: Hello Patsy, Thank you very much for responding! Yes of course, I'll be removing the celloidin from each section - we normally do this on smaller human whole brainstem sections using ether/100% EA 1:1 - 3x - 3 minutes each with agitation. The latter type of sections are very easy to work with, however these future big boys I'll be getting is another thing. Our lab is currently alcohol processing a human whole brain after the last 95% EA - it will be embedded in 2% celloidin placed inside a very large desiccator under 20 psi pressure. This part like every step will take some period of time, I need to test several different IHC methods hope one will actually work. If you have any further ideas or thoughts on this subject - shoot me an email. Thank you again for responding. Maria -- *From:* Patsy Ruegg [prueg...@hotmail.com] *Sent:* Sunday, January 04, 2015 7:03 PM *To:* Jay Lundgren; Maria Mejia *Cc:* Histonet@Lists. Edu; Mejia, Mary *Subject:* RE: [Histonet] HELP! Need some old fashioned histology advice I have done something similar to this but I used tissue that was fixed but not processed and embedded, this is called enblock labeling, I infiltrated the fixed tissue with the IHC reagents, in a vial/tube, the blocking reagents, then the antibody, then the detection reagents and DAB, then dehydrated the tissue. I used vials or tubes on a platform shaker and would infiltrate reagents for days, then after it was done I infiltrated and embedded the tissue in glycol methacrylate (GMA) so that I could section it, it actually worked. The tissue was already IHc LABeled so all I did to the 5 micron sections after they were cut was a hematoxylin counterstain, this was mineralized bone so I had to embedd in something hard like GMA to section. Will you remove the Celloidin before trying to do the IHC staining? 100 micron sections might be easy to float/handle using a glass pipette for transferring. Sounds like an interesting project, good luck and feel free to ask for advise and keep us posted on your progress. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com Date: Sun, 4 Jan 2015 11:58:38 -0600 From: jaylundg...@gmail.com To: mbmph...@gmail.com Subject: Re: [Histonet] HELP! Need some old fashioned histology advice CC: histonet@lists.utsouthwestern.edu; mary.me...@ucsf.edu I can help with the old fashioned advice: - 1 scant teaspoon simple syrup - 2 dashes Angostura Bitters, plus more to taste - 1 half dollar–sized slice orange peel, including pith - 2 ounces good-quality rye or bourbon - 1 maraschino cherry As for the Histology, is there any reason you cannot mount the sections onto glass slides? When I was working at Genentech they were cutting frozen sections through whole rabbits and mounting the sections on (giant) glass slides. I think that rolling the tissue up, inserting it, and then removing it from a glass tube would destroy the tissue. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Sat, Jan 3, 2015 at 8:01 PM, Maria Mejia mbmph...@gmail.com wrote: First, the very best of holidays to everyone. Now for the histology part. Our lab's focus is on the early stages of Alzheimer's Disease in the Brainstem using celloidin processing embedding for IHC staining. This year, our lab will be receiving 6 post-mortem whole human brains (1 every other month). After fixation, processing celloidin embedding, the whole brain will be serially cut at 100um thick. Each brain section will be 5
[Histonet] Automatic H E slide stainer recommendations
Hello colleagues, I would appreciate any recommendations for an automatic slide stainer. It will primarily be used for H E staining, not IHC. Thanks in advance, Carla Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street Seattle, WA 98115-5016 USA Phone: 206-526-2042 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: And other crazy stuff. RE: [Histonet] cutting honey bees
When I worked in a research core (which was only last week, but I just changed jobs). I have processed and cut (with varying degrees of success) fake meat samples for a company. They were mainly made of grains and mushed veggies. The problem was the samples were not consistent and there was no room (time or money) for honing the protocol for each of the many samples he sent me, so he got what I could cut. He always seemed pleased, but I couldn't see much in the samples. He was very secretive about it all, so I could never quite understand what they were doing t all for!!! C Sent from my iPhone On Jan 6, 2015, at 12:54 PM, Roberta Horner r...@psu.edu wrote: The oddest things I cut were the honey bee and yellow jacket stingers. I've done plant stamen, reptiles, fish and I believe another insect. I usually tell the students that are working on a research project to give me a sample they don't care about so I can see if I can do what they want. But I had oddities that I didn't have to section like during hunting season a hunter killed a deer and there was a mass on the trachea that he wanted tested to make sure the deer was okay to eat. I got the sample and when I tried to gross it I found a very hard shiny silver object. I told the pathologist whose case it was that the mass was from a bullet did he still want histo done. No. The other interesting one was the egg shell. The conversation went something like this. Pathologist: Can you section this egg shell Me: No it's too hard. P: Can't you decal it M: That's not going to work. P: Did you try. M: No P: Don't you think you should try first. M: Okay fine but it is no going to work. Put a piece of eggshell (made of calcium) into some decal solution (that removes calcium) and watch the egg shell bubble and disappear. I did get to tell the pathologist I told you so Roberta Horner Penn State University Animal Diagnostic Lab -Original Message- From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu] Sent: Tuesday, January 06, 2015 2:24 PM To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: And other crazy stuff. RE: [Histonet] cutting honey bees You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] RE: Cost per block on the Sakura Xpress
I have seriously Important Info how to keep your costs as low as possible. We were the first lab to implement this plan and it works perfectly for us! Give me a call for details. Jeanine Sanders CDC Atlanta 404-639-3590 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gowan,Christie C Sent: Tuesday, December 30, 2014 3:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cost per block on the Sakura Xpress Dear Histonetters, Does anyone have an algorithm for cost per block on the Sakura Xpress? I would need it for biopsy specimens only. I inherited one of these machines and before I put it into use, I want to know what it will cost me and yes I have reached out to Sakura (Florida) but no responses as of yet. Many thanks! Christie Gowan HT (ASCP) University of Florida Department of Dermatology 4037 NW 86th Terrace Gainesville, FL 32606 Phone: 352 594-1529 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: HER2 IHC controls
Yes, 3 different antibody expressions per block. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Tuesday, January 06, 2015 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HER2 IHC controls Are any of you making your own HER2 IHC control slides from patient tissue? ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: And other crazy stuff. RE: [Histonet] cutting honey bees
The coolest thing I cut was 600 yo deer bone. One of our pathologist did archeology as a hobby and wanted me to section it, it was petrified as hard as a rock. We tried everything to soften it to no avail. We ended up cutting with a diamond wire lapidary saw without embedding it in anything. Could make sections as thin as 30 microns. Had a heck of a time trying to get sections to adhere to a glass slide and the sections would not take any stain. We did end up looking at it with a fluorescent scope. We could see rings like a tree of bone turn over. This is how tetracycline labeling of bone first came about. Someone way back stuck a piece of dinosaur bone under UV light and saw rings, apparently the animals eat moldy grain ( tet is made from mold) and it deposits where ever new bone is being laid down, it also happens to fluoresce. I spent 25 years in a metabolic bone disease lab, we treated the patient with a course of tetracycline then waited for a period of time, I think it was 10-14 days, then the patient took another dose of tet and then within a day or 3 we biopsied their bone usually from the illiac crest fixed it in methanol because the tetracycline was water soluble then processed it into GMA plastic without decal. Unstained 5 micron sections cut with a tungsten carbide blade were reviewed with a fluorescent scope revealing the two labels of tet, since we knew the time between doses we could measure the area between the two labels and report them out as bone growth in mm per day. People with severe lack of bone turn over would just have one single label meaning they were not making much bone. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: timothy.mor...@ucsf.edu; prueg...@hotmail.com; classic...@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: And other crazy stuff. RE: [Histonet] cutting honey bees Date: Tue, 6 Jan 2015 20:54:10 + The oddest things I cut were the honey bee and yellow jacket stingers. I've done plant stamen, reptiles, fish and I believe another insect. I usually tell the students that are working on a research project to give me a sample they don't care about so I can see if I can do what they want. But I had oddities that I didn't have to section like during hunting season a hunter killed a deer and there was a mass on the trachea that he wanted tested to make sure the deer was okay to eat. I got the sample and when I tried to gross it I found a very hard shiny silver object. I told the pathologist whose case it was that the mass was from a bullet did he still want histo done. No. The other interesting one was the egg shell. The conversation went something like this. Pathologist: Can you section this egg shell Me: No it's too hard. P: Can't you decal it M: That's not going to work. P: Did you try. M: No P: Don't you think you should try first. M: Okay fine but it is no going to work. Put a piece of eggshell (made of calcium) into some decal solution (that removes calcium) and watch the egg shell bubble and disappear. I did get to tell the pathologist I told you so Roberta Horner Penn State University Animal Diagnostic Lab -Original Message- From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu] Sent: Tuesday, January 06, 2015 2:24 PM To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: And other crazy stuff. RE: [Histonet] cutting honey bees You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't
Re: [Histonet] PASD muscle stains
Tiffany, Have used 10%NBF on muscles but also alcoholic fixatives -alcoholic formalin or absolute- just always preferred 10%NBF since it gave the morphology and counterstaining I wanted. diastase in a 6.0pH buffer (don't heat above 40 degrees if trying to speed up heating-kill the diastase) and always stayed away from di water on frozen sections. di water too variable and fickle. Ray Koelling Lake Forest Park - Original Message - From: Tiffany Passaro tpass...@cellnetix.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Sent: Monday, January 5, 2015 3:22:32 PM Subject: [Histonet] PASD muscle stains Greetings, I am looking for fixatives that others are using in their labs for the PASD stain on fresh frozen muscle tissue. Currently we are fixing in 10% NBF. Thanks in advance for any info on this. Tiffany DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: And other crazy stuff. RE: [Histonet] cutting honey bees
The oddest things I cut were the honey bee and yellow jacket stingers. I've done plant stamen, reptiles, fish and I believe another insect. I usually tell the students that are working on a research project to give me a sample they don't care about so I can see if I can do what they want. But I had oddities that I didn't have to section like during hunting season a hunter killed a deer and there was a mass on the trachea that he wanted tested to make sure the deer was okay to eat. I got the sample and when I tried to gross it I found a very hard shiny silver object. I told the pathologist whose case it was that the mass was from a bullet did he still want histo done. No. The other interesting one was the egg shell. The conversation went something like this. Pathologist: Can you section this egg shell Me: No it's too hard. P: Can't you decal it M: That's not going to work. P: Did you try. M: No P: Don't you think you should try first. M: Okay fine but it is no going to work. Put a piece of eggshell (made of calcium) into some decal solution (that removes calcium) and watch the egg shell bubble and disappear. I did get to tell the pathologist I told you so Roberta Horner Penn State University Animal Diagnostic Lab -Original Message- From: Morken, Timothy [mailto:timothy.mor...@ucsf.edu] Sent: Tuesday, January 06, 2015 2:24 PM To: Patsy Ruegg; Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: And other crazy stuff. RE: [Histonet] cutting honey bees You crazy research people...OK, so what is the craziest thing you ever had to cut, or were asked to cut? For me, not too bad, but embedding for EM and sectioning a single oocyte that was nearly microscopic. I'll just say it took a LOT of thick sections too face down to it without actually cutting through it. Open the floodgates Tim Morken -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Tuesday, January 06, 2015 11:13 AM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] cutting honey bees
I processed honeybees and was successful sectioning them. It takes a bit of patience and time. I soaked the bees to soften the outer parts in glycerin water and or mollifex. As for processing, I had to make up a schedule and infiltration is the key. If it isn't important to see the whole bee you can section out the parts you need to see most and then it becomes easy. I'm slowly working on my notes and someday I'll get them all sorted out. Andi G From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Patsy Ruegg [prueg...@hotmail.com] Sent: Tuesday, January 06, 2015 12:13 PM To: Roberta Horner; Douglas Gregg; Histonet@Lists. Edu Subject: RE: [Histonet] cutting honey bees for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: r...@psu.edu To: classic...@gmail.com; histonet@lists.utsouthwestern.edu Date: Sat, 3 Jan 2015 23:15:33 + Subject: RE: [Histonet] cutting honey bees CC: I sectioned and stained honey bee and yellow jacket stingers years ago. They wanted to show the difference between the stingers. I wasn't sure what to do so I processed and handled like everything else. I was able to get some good sections. I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked. Roberta Horner Penn State University Animal Diagnostic Lab From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Douglas Gregg [classic...@gmail.com] Sent: Saturday, January 03, 2015 6:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] cutting honey bees Has anyone had experience embedding and cutting honey bees. I am sure there are some issues with the harder exoskeleton. Would that have to be dissected away first. I am considering helping a student with a science fair project on bees. Douglas Gregg Veterianary pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] VIP 6 processing baskets
Does anyone know if the VIP 6 cassette baskets fit other vendor embedding centers? Spec sheets have a lot of measurements but practical experience is the best guide. We are considering a Thermofisher embedding center or a Leica Embedding Center but most likely we will process with a VIP 6 so I want to make sure the baskets will fit. I know that VIP has two sizes of baskets and I am most concerned about the 150 cassette basket. Thanks Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] HELP! Need some old fashioned histology advice
Hello Patsy, Thank you very much for responding! Yes of course, I'll be removing the celloidin from each section - we normally do this on smaller human whole brainstem sections using ether/100% EA 1:1 - 3x - 3 minutes each with agitation. The latter type of sections are very easy to work with, however these future big boys I'll be getting is another thing. Our lab is currently alcohol processing a human whole brain after the last 95% EA - it will be embedded in 2% celloidin placed inside a very large desiccator under 20 psi pressure. This part like every step will take some period of time, I need to test several different IHC methods hope one will actually work. If you have any further ideas or thoughts on this subject - shoot me an email. Thank you again for responding. Maria From: Patsy Ruegg [prueg...@hotmail.com] Sent: Sunday, January 04, 2015 7:03 PM To: Jay Lundgren; Maria Mejia Cc: Histonet@Lists. Edu; Mejia, Mary Subject: RE: [Histonet] HELP! Need some old fashioned histology advice I have done something similar to this but I used tissue that was fixed but not processed and embedded, this is called enblock labeling, I infiltrated the fixed tissue with the IHC reagents, in a vial/tube, the blocking reagents, then the antibody, then the detection reagents and DAB, then dehydrated the tissue. I used vials or tubes on a platform shaker and would infiltrate reagents for days, then after it was done I infiltrated and embedded the tissue in glycol methacrylate (GMA) so that I could section it, it actually worked. The tissue was already IHc LABeled so all I did to the 5 micron sections after they were cut was a hematoxylin counterstain, this was mineralized bone so I had to embedd in something hard like GMA to section. Will you remove the Celloidin before trying to do the IHC staining? 100 micron sections might be easy to float/handle using a glass pipette for transferring. Sounds like an interesting project, good luck and feel free to ask for advise and keep us posted on your progress. Cheers, Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com Date: Sun, 4 Jan 2015 11:58:38 -0600 From: jaylundg...@gmail.com To: mbmph...@gmail.com Subject: Re: [Histonet] HELP! Need some old fashioned histology advice CC: histonet@lists.utsouthwestern.edu; mary.me...@ucsf.edu I can help with the old fashioned advice: - 1 scant teaspoon simple syrup - 2 dashes Angostura Bitters, plus more to taste - 1 half dollar–sized slice orange peel, including pith - 2 ounces good-quality rye or bourbon - 1 maraschino cherry As for the Histology, is there any reason you cannot mount the sections onto glass slides? When I was working at Genentech they were cutting frozen sections through whole rabbits and mounting the sections on (giant) glass slides. I think that rolling the tissue up, inserting it, and then removing it from a glass tube would destroy the tissue. Sincerely, Jay A. Lundgren, M.S., HTL (ASCP) On Sat, Jan 3, 2015 at 8:01 PM, Maria Mejia mbmph...@gmail.com wrote: First, the very best of holidays to everyone. Now for the histology part. Our lab's focus is on the early stages of Alzheimer's Disease in the Brainstem using celloidin processing embedding for IHC staining. This year, our lab will be receiving 6 post-mortem whole human brains (1 every other month). After fixation, processing celloidin embedding, the whole brain will be serially cut at 100um thick. Each brain section will be 5 inches x 4.5 inches in size. I will given 250 of these whole brain sections to stain for tau IHC...that's 1500 whole brain sections/year!!! 1) Does anyone have experience doing manual IHC staining of large free-floating brain sections? 2) What type of staining tools, dishes or other essential equipment can anyone recommend? 3) What's the most efficient way to stain 250 sections for batch IHC staining - such as transferring batch sections (maybe 5-10) from reagent to reagent? 4) What type of batch apparatus to use? As for the antibody ABC steps, I was thinking of placing each section inside a large glass cigar tube (yep, people use large glass tubes with fitted cap to store cigars), with 5ml of antibody or ABC reagent gently agitate on a shaker/rotator at room temp during the incubation. Does anyone have ideas on this? Please, any ideas, suggestions or recommendation anyone can provide will be most greatly appreciated. Best regards Maria Mejia UCSF Department of Neurology San Francisco, CA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu
[Histonet] PASD muscle stains
Greetings, I am looking for fixatives that others are using in their labs for the PASD stain on fresh frozen muscle tissue. Currently we are fixing in 10% NBF. Thanks in advance for any info on this. Tiffany DISCLAIMER: This message is intended for the sole use of the addressee, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If you are not the addressee you are hereby notified that you may not use, copy, disclose, or distribute to anyone the message or any information contained in the message. If you have received this message in error, please immediately advise the sender by reply email and delete this message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Full Time HT position in Los Angeles, CA
PATH MD, a state-of-the-art reference pathology laboratory located in Los Angeles, CA, is looking for two full time histotechnicians to start sometime in the first quarter of 2015. Applicant must be a motivated team player and have at least one year of experience and be ASCP registered. Proficiency in embedding, cutting and special stains is required. PATH MD offers full medical, dental, and vision benefits as well as a 401(k) plan. Interested applicants may send their resumes to: lcolb...@pathmdlabs.com Laurie Colbert, HT (ASCP) Histology Supervisor PATH MD 8158 Beverly Blvd. Los Angeles, CA 90048 (323) 648-3214 direct (424) 245-7284 main lab The information contained in this transmission may contain privileged and confidential information, including patient information protected by federal and state privacy laws. It is intended only for the use of the person(s) named above. If you are not the intended recipient, you are hereby notified that any review, dissemination, distribution, or duplication of this communication is strictly prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] HER2 IHC controls
Are any of you making your own HER2 IHC control slides from patient tissue? ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
