[Histonet] MMA Troubleshooting for Undecalcified Bone
This is intended for Bone Histology samples embedded in MMA. I had two main questions. 1. After mounting my tissue sections (5-10microns) with Haupts adhesive, I noticed that the bone within the section was appearing opaque/darkened on the microscope? I will add that the morphology of the bone structure is not present. The bone still stains, but the detail is missing. I am thinking that I might of over dehydrated during processing or my microtome blade is dull. Any suggestions about this problem would be appreciated. 2. Anyone have a Hematoxylin Eosin Staining protocol for undecalcified MMA embedded bone? I do have a protocol, but not sure if I was missing a couple of steps for proper staining. Thank you all. Regards, Jesse Hernandez ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] marking small samples
Dear Histonetters, Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? Preferably something that would not interfere with subsequent antibody staining. Yves ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] marking small samples
Eosin in the alcohol works great to mark these small pieces of tissue. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com mailto:jmcgo...@clinlab.com www.clinlab.com http://www.clinlab.com -Original message- From:Yves Heremans yves.herem...@vub.ac.be mailto:yves.herem...@vub.ac.be Sent: Wednesday, January 14, 2015 3:11 PM To: histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] marking small samples Dear Histonetters, Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? Preferably something that would not interfere with subsequent antibody staining. Yves ___ Histonet mailing list Histonet@lists.utsouthwestern.edu mailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] marking small samples
We use eosin the processor alcohol. Also we use blue-tinted paraffin which helps with contrast on small bx Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yves Heremans Sent: Wednesday, January 14, 2015 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking small samples Dear Histonetters, Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? Preferably something that would not interfere with subsequent antibody staining. Yves ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] marking small samples
I've been told Eosin affects a lot of molecular testing. We used to use it in our process but discontinued to not interfere with future testing. Sent from my iPhone On Jan 14, 2015, at 5:31 PM, Morken, Timothy timothy.mor...@ucsf.edu wrote: We use eosin the processor alcohol. Also we use blue-tinted paraffin which helps with contrast on small bx Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yves Heremans Sent: Wednesday, January 14, 2015 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking small samples Dear Histonetters, Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? Preferably something that would not interfere with subsequent antibody staining. Yves ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Of possible interest to some Histonet Listers
Dear Histonet Listers: The following link, http://blaypublishers.com/category/previous-issues/ , contains the hitherto published issues of a peer-reviewed, scientific journal I run, called* Life: The Excitement of Biology*. Some of the articles deal with subjects of possible interest to some of you. Sincerely, Jorge Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html Jorge A. Santiago-Blay, PhD blaypublishers.com http://blayjorge.wordpress.com/ http://paleobiology.si.edu/staff/individuals/santiagoblay.html ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Message to renew subscription.
Got a message to renew subscription or it will be cut off.? Would like to continue receiving histonet.? Is this message from some criminal or from histonet?? Alice Neumann MD Pinnacle Pathology PC 207 Auburn Dr. ?#1 Auburn, AL 36830 24 Hour Cell Phone:? 307-413-4092 alineum...@aol.com ? ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] marking small samples
Eosin can be a problem in IHC as well especially if you use alk phos red detection. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: mbu...@atlanticurologyclinics.com To: timothy.mor...@ucsf.edu Date: Wed, 14 Jan 2015 22:56:51 + Subject: Re: [Histonet] marking small samples CC: histonet@lists.utsouthwestern.edu; yves.herem...@vub.ac.be I've been told Eosin affects a lot of molecular testing. We used to use it in our process but discontinued to not interfere with future testing. Sent from my iPhone On Jan 14, 2015, at 5:31 PM, Morken, Timothy timothy.mor...@ucsf.edu wrote: We use eosin the processor alcohol. Also we use blue-tinted paraffin which helps with contrast on small bx Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yves Heremans Sent: Wednesday, January 14, 2015 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking small samples Dear Histonetters, Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? Preferably something that would not interfere with subsequent antibody staining. Yves ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] marking small samples
Thanks for your research Tim, this is good to know. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: timothy.mor...@ucsf.edu To: mbu...@atlanticurologyclinics.com Date: Wed, 14 Jan 2015 23:28:49 + Subject: RE: [Histonet] marking small samples CC: histonet@lists.utsouthwestern.edu Last year I found a paper to the effect that eosin may interfere with PCR analysis. Our molecular group did an internal study and did not find any significant issues. But I just found another paper stating no interference: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3383644/ Am J Clin Pathol. Jul 2012; 138(1): 122-129. No Evidence for Interference of Hematoxylin and Eosin (HE) Staining in DNA Testing: Utility of DNA Extraction from HE-Stained Archival Tissue Sections Teppei Morikawa, MD im Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: Melissa Burns [mailto:mbu...@atlanticurologyclinics.com] Sent: Wednesday, January 14, 2015 2:57 PM To: Morken, Timothy Cc: Yves Heremans; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] marking small samples I've been told Eosin affects a lot of molecular testing. We used to use it in our process but discontinued to not interfere with future testing. Sent from my iPhone On Jan 14, 2015, at 5:31 PM, Morken, Timothy timothy.mor...@ucsf.edu wrote: We use eosin the processor alcohol. Also we use blue-tinted paraffin which helps with contrast on small bx Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yves Heremans Sent: Wednesday, January 14, 2015 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking small samples Dear Histonetters, Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? Preferably something that would not interfere with subsequent antibody staining. Yves ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] New Histology Opportunities - CA
Hello Histonet! We hope everyone is having a great 2015. One of our leading clients has opened a few Field Based Histology Support opportunities based on the west coast. The ideal candidate will have an IHC background and interested in a customer facing support/specialist type opportunity. The position offers a very competitive package including a salary, bonus, company car, cell phone, laptop, full benefits. If this sounds like something you may be interested in learning more about, please contact me directly at m...@personifysearch.com mailto:m...@personifysearch.com . Thanks! Matt Matt Ward Program Manager Personify 5020 Weston Parkway Suite 315 Cary NC 27513 (Tel) 919.459.3654 (Tel) 800.875.6188 direct ext 103 (Fax) 919.882.8727 http://www.personifysearch.com/ www.personifysearch.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] marking small samples
Last year I found a paper to the effect that eosin may interfere with PCR analysis. Our molecular group did an internal study and did not find any significant issues. But I just found another paper stating no interference: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3383644/ Am J Clin Pathol. Jul 2012; 138(1): 122-129. No Evidence for Interference of Hematoxylin and Eosin (HE) Staining in DNA Testing: Utility of DNA Extraction from HE-Stained Archival Tissue Sections Teppei Morikawa, MD im Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: Melissa Burns [mailto:mbu...@atlanticurologyclinics.com] Sent: Wednesday, January 14, 2015 2:57 PM To: Morken, Timothy Cc: Yves Heremans; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] marking small samples I've been told Eosin affects a lot of molecular testing. We used to use it in our process but discontinued to not interfere with future testing. Sent from my iPhone On Jan 14, 2015, at 5:31 PM, Morken, Timothy timothy.mor...@ucsf.edu wrote: We use eosin the processor alcohol. Also we use blue-tinted paraffin which helps with contrast on small bx Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Yves Heremans Sent: Wednesday, January 14, 2015 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] marking small samples Dear Histonetters, Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ? Preferably something that would not interfere with subsequent antibody staining. Yves ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] p16 Antibody
Ventana unfortunately has the market on the good clone...you can dilute it out pretty far (it comes pre-diluted) and still get good results however... Sarah Dysart BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor North Austin Medical Center 12221 North Mopac Expressway Austin, TX 78758 512-901-1220 (lab) 512-901-6659 (office) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, January 14, 2015 8:41 AM To: 'Véronique Barrès'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Antibody We use one from BD, Veronique, with good results. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès Sent: Wednesday, January 14, 2015 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Antibody Hi Histonetters! I am working in a research lab and we sometimes need to do p16 staining, but our pathologist told us that the antibody we are using right now is not good. I'd like to know which antibody you're using in your labs? Thanks for your help and happy Wednesday! Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] p16 Antibody
We use one from BD, Veronique, with good results. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès Sent: Wednesday, January 14, 2015 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Antibody Hi Histonetters! I am working in a research lab and we sometimes need to do p16 staining, but our pathologist told us that the antibody we are using right now is not good. I'd like to know which antibody you're using in your labs? Thanks for your help and happy Wednesday! Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] p16 Antibody
We use the Ventana (INK4a) clone as well. What dilution do you use? Brandy Burnett Histotechnoligist, QIHC(ASCP) CCH Pathology/Histology 508-862-5267 bburn...@capecodhealth.org Expert physicians. Quality hospitals. Superior care. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah.dys...@stdavids.com Sent: Wednesday, January 14, 2015 10:01 AM To: lseb...@uwhealth.org; veronique.bar...@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Antibody Ventana unfortunately has the market on the good clone...you can dilute it out pretty far (it comes pre-diluted) and still get good results however... Sarah Dysart BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor North Austin Medical Center 12221 North Mopac Expressway Austin, TX 78758 512-901-1220 (lab) 512-901-6659 (office) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, January 14, 2015 8:41 AM To: 'Véronique Barrès'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Antibody We use one from BD, Veronique, with good results. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès Sent: Wednesday, January 14, 2015 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Antibody Hi Histonetters! I am working in a research lab and we sometimes need to do p16 staining, but our pathologist told us that the antibody we are using right now is not good. I'd like to know which antibody you're using in your labs? Thanks for your help and happy Wednesday! Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] MYC-N antibody
Good Morning, I am wondering if anyone in the clinical world is using MYC-N antibody? I would like to know who the manufacturer is and what your protocol may be. I will be running these in a research environment, first on cells and then potentially on research tissue. Your help will be greatly appreciated! Have a great day! Kind Regards! John J Shelley Research Specialist, Histology Core Facility Sanford-Burnham Medical Research Institute at Lake Nona 6400 Sanger Road Orlando, FL 32827 Tel: (407) 745-2000 Ext.2517 Lab: (407) 745-2119 Fax: (407) 745-2001 email: jshel...@sanfordburnham.orgmailto:jshel...@sanfordburnham.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] breast tissue and radioactive seeds
Hi Everyone, After 5 years of being in the private sector I am now back in the wonderful environment of a hospital setting as the Histology supervisor. We are going to be starting a new procedure here called Conversion to Radioactive Seed Localized Breast Surgery and I have been asked to come to this meeting. As I have been strictly in Dermatology for the past few years I am not familiar with this process. I have dealt with the prostate radioactive seeds before but not sure if this will involve the Histology lab in the form of surgical tissue or not. I would like to be as informed as possible before the meeting which is next week, so I came to the source I value the most. Can anyone share any information with me? Thanks so much, Kaye ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] p16 Antibody
We dilute ours 1:5 from the predilute and run it on the Bond 3. It works very well. Martha Ward, MT (ASCP) QIHC Manager Molecular Diagnostics Lab Medical Center Boulevard \ Winston-Salem, NC 27157 p 336.716.2109 \ f 336.716.5890 mw...@wakehealth.edu -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Burnett, Brandy Sent: Wednesday, January 14, 2015 10:15 AM To: 'sarah.dys...@stdavids.com'; lseb...@uwhealth.org; veronique.bar...@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Antibody We use the Ventana (INK4a) clone as well. What dilution do you use? Brandy Burnett Histotechnoligist, QIHC(ASCP) CCH Pathology/Histology 508-862-5267 bburn...@capecodhealth.org Expert physicians. Quality hospitals. Superior care. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sarah.dys...@stdavids.com Sent: Wednesday, January 14, 2015 10:01 AM To: lseb...@uwhealth.org; veronique.bar...@gmail.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Antibody Ventana unfortunately has the market on the good clone...you can dilute it out pretty far (it comes pre-diluted) and still get good results however... Sarah Dysart BA, HT (ASCP), QIHC (ASCP) Pathology Supervisor North Austin Medical Center 12221 North Mopac Expressway Austin, TX 78758 512-901-1220 (lab) 512-901-6659 (office) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A Sent: Wednesday, January 14, 2015 8:41 AM To: 'Véronique Barrès'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] p16 Antibody We use one from BD, Veronique, with good results. Linda A. Sebree University of Wisconsin Hospital Clinics IHC/ISH Laboratory, Rm A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Véronique Barrès Sent: Wednesday, January 14, 2015 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 Antibody Hi Histonetters! I am working in a research lab and we sometimes need to do p16 staining, but our pathologist told us that the antibody we are using right now is not good. I'd like to know which antibody you're using in your labs? Thanks for your help and happy Wednesday! Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ~~ This email and any files transmitted with it are confidential, and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error contact the Help Desk for Cape Cod Healthcare. helpd...@capecodhealth.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Tissue exchange- Coccidiodes for fungus
I checked with NSH and they say that their tissue exchange bank has been discontinued. I have a lot of lung tissue containing Coccidiodes organisms (Valley Fever). I would like to trade for some tissue containing fungus. Is there another tissue exchange network out there? Is anyone interested in trading? Hi Tim. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Tissue exchange- Coccidiodes for fungus
Hi Jeff, The Michigan Society of Histotechnologist has a tissue exchange bank available to all MSH members, so you might want to check with them. Regards, Vincent Rivera, HT (ASCP) QIHC, QLS Histopathology Supervisor West Dermatology Pathology Laboratory vriv...@westderm.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Wednesday, January 14, 2015 8:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Tissue exchange- Coccidiodes for fungus I checked with NSH and they say that their tissue exchange bank has been discontinued. I have a lot of lung tissue containing Coccidiodes organisms (Valley Fever). I would like to trade for some tissue containing fungus. Is there another tissue exchange network out there? Is anyone interested in trading? Hi Tim. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: breast tissue and radioactive seeds
If this is the I-125 seed localization procedure for breast casses: our Radiology dept here does this and I can tell you what we do here. Becky Garrison Pathology Supervisor Shands Jacksonville Jacksonville, FL 32209 904-244-6237, phone 904-244-4290, fax 904-393-3194, pager -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of robin.r...@hcahealthcare.com Sent: Wednesday, January 14, 2015 10:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] breast tissue and radioactive seeds Hi Everyone, After 5 years of being in the private sector I am now back in the wonderful environment of a hospital setting as the Histology supervisor. We are going to be starting a new procedure here called Conversion to Radioactive Seed Localized Breast Surgery and I have been asked to come to this meeting. As I have been strictly in Dermatology for the past few years I am not familiar with this process. I have dealt with the prostate radioactive seeds before but not sure if this will involve the Histology lab in the form of surgical tissue or not. I would like to be as informed as possible before the meeting which is next week, so I came to the source I value the most. Can anyone share any information with me? Thanks so much, Kaye ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Cassette lableler and slide labeler
Jim, by system do you mean using General Data software/printer to produce the barcodes, outside of your LIS, or just using the cassette printer with your LIS? If you are using a third party system to track materials outside of the LIS, then everything downstream has to use that system as well, assuming the LIS does produce the codes, so cannot recognize them. In that case all tracking is in a system outside the LIS. However, using a different company's slide printer with the third party system will not be an issue if there is a print driver for the third party software to drive that printer. If they have to develop a print driver, that could cost you some . Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Tuesday, January 13, 2015 7:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette lableler and slide labeler I didn't plan on this however wondered if anyone had a General Data system for making cassettes but a different setup for making slides, such as a Thermo Slidemate. I suspect there are issues with the LIS system, barcoding, etc.Am I wrong? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Cassette lableler and slide labeler
James, I think I can respond to this as I am an LIS vendor. The short answer is that it should NOT be an issue, ASSUMING your LIS vendor can communicate/interface with the two instruments. Once the barcode is on the cassette or the slide, it becomes a standalone entity. The barcode on the cassette or slide then gets read by a barcode scanner. Every scanner I've run across can read multiple symbologies (bar code types, if you will). Some scanners read better than others, though. I am assuming that you're generating cassettes and slides through your LIS. If you're using the cassette/slide printer vendor's software to do this, then that changes things. If you'd like further clarification or discussion, feel free to email me. Michael Mihalik PathView Systems | cell: 214.733.7688 | 800.798.3540 | fax: 952.241.7369 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Vickroy, James Sent: Tuesday, January 13, 2015 7:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cassette lableler and slide labeler I didn't plan on this however wondered if anyone had a General Data system for making cassettes but a different setup for making slides, such as a Thermo Slidemate. I suspect there are issues with the LIS system, barcoding, etc.Am I wrong? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.commailto:jvick...@springfieldclinic.com This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] p16 Antibody
Hi Histonetters! I am working in a research lab and we sometimes need to do p16 staining, but our pathologist told us that the antibody we are using right now is not good. I'd like to know which antibody you're using in your labs? Thanks for your help and happy Wednesday! Véronique ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Texas Society for Histotechnology 2015 Sympsoium Convention March 20-22, 2015
Hi Histonetters! The Texas Society for Histotechnology will be holding the 2015 S/C in Plano Texas. If you are interested in a electronic version of the program please send me a e-mail via this communication. We would love to have you join us this year! Regards, Kathy Dwyer Texas Society for Histotechnology 37th Annual Convention and Symposium Texas Histology: A Wealth ofKnowledge Dallas/Plano Marriott at Legacy TownCenter Plano, Texas March 20-22, 2015 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
