[Histonet] slide folders for double wide slides
Hi All, What slide folders do any of you recommend for use with 2x3 slides (we use them for whole-mount prostates)? If you have some that work well, could you send me the ordering info? Thanks, Bonnie Bonnie Whitaker AP Operations Director The Ohio State University Wexner Medical Center Department of Pathology N305 Doan Hall 410 West 10th Avenue Columbus, Ohio 43210 614.293.8418 FAX 614.293.2779 Pager: 614.293.7243 ext. 5013 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Utah Society
Utah is in NSH region 7 and your regional director is Jane Parr, jane.p...@ucdenver.edu . I would contact her to double check. Terra Wineman, HTL (ASCP)CM Research Biologist, Nutritional Physiology 636-926-7476 phone terra.wine...@novusint.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kimberly Marshall Sent: Tuesday, February 24, 2015 3:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Utah Society ?Hello Histo folks. Are there any Utah Histo Tech subscribing or anyone with the information that Utah has a Histo society? Would really like info. Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] sections not sticking to charged slides
Help, sections not sticking to charged slides. We use Mercedes Medical charged slides. Lately have been using sta-on but still have occasional problems especially during antigen retrieval. I need any suggestions. Renee H. Workman Histology Supervisor Virginia Urology 9105 Stony Point Drive Richmond, VA 23235 W: 804-527-1316 | F: 804-270-0917 rhwork...@uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: sections not sticking to charged slides
Hi Renee, Hope you don't mind but I have had a lot of experience in this area. I worked for Ventana for 7 1/2 years as technical specialist and now as a Technical Support Manager and I know that getting sections to stick to slides can be a bit challenging especially during antigen retrieval. One of the rules when using positively charged slides is to not use any type of adhesive in the water bath, such as Sta-On only use distilled water. The use of such products actually works against you when trying to get the sections adhered to slides. Also I would say that when doing IHC, something to keep in mind is that adhesive properties of the slides vary over time, so age of the slides is a factor and also exposure to humidity and heat is another factor which can affect the adhesive properties of the slides. Make sure that you are not buying too many slides at any one time and that you go through them in no more than 6 months. Also if cutting controls and storing in a plastic box that can mean that the slides sit around for awhile before being used, so don't cut too many controls at one time. I would suggest drying the slides for at least 30 minutes to 1 hour at 60C and if you are getting trapped water under the section, you may need to dry longer to make sure that all the water is removed. If you would like to sample some of the StatLab slides that we feel are very good for IHC, please let myself or Kevin Collins know, we would be happy to sample them if you haven't already tried them. If I can help in any other way, please let me know. Debbie Siena, HT(ASCP)QIHC StatLab Medical Products Technical Support Manager 407 Interchange Street | McKinney, TX 75071 t: 800.442.3573 ext. 229 | f: 972.767.3992 dsi...@statlab.com | www.statlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Renee H. Workman Sent: Tuesday, February 24, 2015 3:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sections not sticking to charged slides Help, sections not sticking to charged slides. We use Mercedes Medical charged slides. Lately have been using sta-on but still have occasional problems especially during antigen retrieval. I need any suggestions. Renee H. Workman Histology Supervisor Virginia Urology 9105 Stony Point Drive Richmond, VA 23235 W: 804-527-1316 | F: 804-270-0917 rhwork...@uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Utah Society
?Hello Histo folks. Are there any Utah Histo Tech subscribing or anyone with the information that Utah has a Histo society? Would really like info. Thanks in advance ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] How dark is dark enough?
As long as your microscope has a good Hg source light, it is not really necessary to darken the room so much. I used to have a very small room were the microscope was located but later on we moved the microscope to another area were we did not dim the light at all and the vision was OK.Everything depends on the circumstances and how comfortable you are with the image.There are no rules on this subject.René J. On Tuesday, February 24, 2015 10:21 AM, Paula Sicurello pat...@gmail.com wrote: Good Morning Netters, While running immunofluorescence stains, how dark is dark enough? I have worked in labs where we did them in full room light, almost complete darkness (developing negative/film darkness), and somewhere in between. I feel that dimmed lights are good enough. What does the histology collective think? Thanks in advance! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [EXTERNAL] Re: [Histonet] gelatin
That's interesting, I didn't realize gelatin is the water soluable protein of collagen. No experience with staining gelatin, but have you considered MassonTrichrome. It is used to differentiate collagen from smooth muscle... -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Tuesday, February 24, 2015 12:48 AM To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] Re: [Histonet] gelatin You need to explain treated tissue. Gelatin is collagen that has been boiled until the protein has lost all its fibrous nature and changed into a water-soluble protein. Gelatin is made permanently insoluble by adequate formaldehyde fixation. It is stained by anionic dyes (such as eosin in the HE method), but it does not show as fibres when you look at the section or smear through a microscope. If this doesn't answer your question, please explain your problem and involve your boss in future email exchanges. John Kiernan London, Canada = = = On 23/02/15, Yak-Nam Wang ynw...@u.washington.edu wrote: Hello, Does anyone know of a stain specific for gelatin? I would like to distinguish between firbous collagen and gelatin in treated tissue. thank you Yak-Nam University of Washington Seattle, WA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] How dark is dark enough?
Good Morning Netters, While running immunofluorescence stains, how dark is dark enough? I have worked in labs where we did them in full room light, almost complete darkness (developing negative/film darkness), and somewhere in between. I feel that dimmed lights are good enough. What does the histology collective think? Thanks in advance! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] gelatin
Thank you for your e-mail. Apologies for not explaining treated tissue. We treat the tissue with high intensity focused ultrasound. It can raise the temperature of tissue to boiling in a localized area (millimeter areas). I could use a biochemical assay for collagen and gelatin if we treat a large area, but with single lesions I was hoping I could visualize this. In some treated areas we are almost resulting in liquefaction of the tissue. I am interested to see if we are turning the collagen to gelatin in these areas and what part of the lesion this is happening. Thank you for your thoughts Yak-Nam On Mon, Feb 23, 2015 at 9:47 PM, John Kiernan jkier...@uwo.ca wrote: You need to explain treated tissue. Gelatin is collagen that has been boiled until the protein has lost all its fibrous nature and changed into a water-soluble protein. Gelatin is made permanently insoluble by adequate formaldehyde fixation. It is stained by anionic dyes (such as eosin in the HE method), but it does not show as fibres when you look at the section or smear through a microscope. If this doesn't answer your question, please explain your problem and involve your boss in future email exchanges. *John Kiernan* London, Canada = = = On 23/02/15, *Yak-Nam Wang * ynw...@u.washington.edu wrote: Hello, Does anyone know of a stain specific for gelatin? I would like to distinguish between firbous collagen and gelatin in treated tissue. thank you Yak-Nam University of Washington Seattle, WA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Creutzfeldt-Jakob Disease
Hello to all in histoland. Does anyone have a procedure for handling creutzfeldt-jakob disease. Any help will be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Creutzfeldt-Jakob Disease
Hi Allison, I would suggest going to the CDC website and pulling from there, they should have the latest recommendations. thanks Debbie Siena 800.442.3573 ext. 229 | www.statlab.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Tuesday, February 24, 2015 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Creutzfeldt-Jakob Disease Hello to all in histoland. Does anyone have a procedure for handling creutzfeldt-jakob disease. Any help will be appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital 713-566-5287(Lab) 713-566-2148(Office) CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 (HIPAA), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] How dark is dark enough?
If you are using an automated stainer the plexiglas cover that they all have will block UV light. For instance we run all our IF in a Dako stainer with the slightly tinted Plexiglas and have not had any problems. If staining manually in a tray, covering with something to block light is ok. On the other hand, I can't really say I've had a problem even with no light blocking. The incubations are usually so short it may not make a difference. Consider that the focused UV light in the fluorescence scope is thousands of times stronger than any overhead tube and it still takes a while for the signal to dim. Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Tuesday, February 24, 2015 7:21 AM To: HistoNet Subject: [Histonet] How dark is dark enough? Good Morning Netters, While running immunofluorescence stains, how dark is dark enough? I have worked in labs where we did them in full room light, almost complete darkness (developing negative/film darkness), and somewhere in between. I feel that dimmed lights are good enough. What does the histology collective think? Thanks in advance! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] CBG recycler
Hello histonet, Has anyone out there dealt with a busted Heat Resistor in a CBG reagent recycler. Any thoughts or insights appreciated. Thanks in advance, Ryan Roy HTL (ASCP) Manchester Veterans Affairs Medical Center Manchester New Hampshire Disclosure: The content of this email does not represent the views or opinons of the VA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Re: gelatin
Thank you Dr. Kiernan, Nice and scientific explanation how to prepare gelatin. I beleive that a lot of house wives and cooks know how to prepare meet or fish jelly (long bouling of the bones with cartilage and tendons). very popular in russian or easter europian cuisine, called cholodez which neans cold.best regards Galina DeynekoNovartis, Cambridge, MA 617-871-7613 w From: histonet-requ...@lists.utsouthwestern.edu histonet-requ...@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Wednesday, December 31, 1969 7:00 PM Subject: Histonet Digest, Vol 135, Issue 25 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. Re: Storing antibodies in a frostless freezer (Teri Johnson) 2. Re: gelatin (John Kiernan) 3. RE: [EXTERNAL] Re: [Histonet] gelatin (Roy, Ryan) 4. How dark is dark enough? (Paula Sicurello) 5. Re: gelatin (Yak-Nam Wang) 6. Re: How dark is dark enough? (Rene J Buesa) 7. Creutzfeldt-Jakob Disease (Scott, Allison D) 8. RE: Creutzfeldt-Jakob Disease (Debra Siena) 9. RE: How dark is dark enough? (Morken, Timothy) 10. CBG recycler (Roy, Ryan) -- Message: 1 Date: Mon, 23 Feb 2015 18:28:54 + From: Teri Johnson tejohn...@genoptix.com Subject: [Histonet] Re: Storing antibodies in a frostless freezer To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: f1d2836e336c4479936999f03ac57...@phuscb-sp37mb04.genoptix.org Content-Type: text/plain; charset=WINDOWS-1252 I agree with Rachel. I would not be quite as worried about the temperature change if indeed they maintain no higher than -13 degrees C and would not be freeze/thawing, but what does your ice cube tray look like after about a month or more in a frost-free freezer? Best wishes, Teri Johnson, HT(ASCP)QIHC Genoptix, Inc. A Novartis Company Carlsbad, CA CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and contains information that is confidential and proprietary to Genoptix Medical Laboratory or its subsidiaries. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, immediately contact the sender by e-mail and destroy all copies of the original message. -- Message: 2 Date: Tue, 24 Feb 2015 00:47:50 -0500 From: John Kiernan jkier...@uwo.ca Subject: Re: [Histonet] gelatin To: Yak-Nam Wang ynw...@u.washington.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 7390897614c8f.54ebc...@uwo.ca Content-Type: text/plain; CHARSET=US-ASCII You need to explain treated tissue. Gelatin is collagen that has been boiled until the protein has lost all its fibrous nature and changed into a water-soluble protein. Gelatin is made permanently insoluble by adequate formaldehyde fixation. It is stained by anionic dyes (such as eosin in the HE method), but it does not show as fibres when you look at the section or smear through a microscope. If this doesn't answer your question, please explain your problem and involve your boss in future email exchanges. John Kiernan London, Canada = = = On 23/02/15, Yak-Nam Wang ynw...@u.washington.edu wrote: Hello, Does anyone know of a stain specific for gelatin? I would like to distinguish between firbous collagen and gelatin in treated tissue. thank you Yak-Nam University of Washington Seattle, WA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Message: 3 Date: Tue, 24 Feb 2015 10:06:11 -0500 From: Roy, Ryan ryan@va.gov Subject: RE: [EXTERNAL] Re: [Histonet] gelatin To: 'John Kiernan' jkier...@uwo.ca, Yak-Nam Wang ynw...@u.washington.edu, histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: 15f883394eab744e99e1c7e1b98730490178a821d...@r04bynmsgb1.r04.med.va.gov Content-Type: text/plain; charset=us-ascii That's interesting, I didn't realize gelatin is the water soluable protein of collagen. No experience with staining gelatin, but have you considered MassonTrichrome. It is used to differentiate collagen from smooth muscle... -Original Message- From: histonet-boun...@lists.utsouthwestern.edu
[Histonet] Is your lab in need of a new microscope?
*Is your lab in need of a new microscope? We have several Olympus BX40 and BX41 compound microscopes in stock! and offer a 1 year warranty on all scopes. * *All microscopes are fully refurbished and are priced at 40% off list. The scopes are serviced by an Authorized Olympus Service Tech before sold. Please Email or call with questions.* -- Scott Munday 90 Misha Lane Sanford, NC 27330 Phone: 919-775-5596 Fax: 919-776-9566 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: sections not sticking to charged slides
HI Renee- I had a major problem in the past with tissue staining for IHC lifting off the slides no matter what I tried. It turned out that when we precut our controls and put them in the oven and then used them later for the patient tissue that the charge on the slide was altered. We currently still precut controls on control slides (we use Leica APEX) and then let them just air dry with no heat. We then use those slides when needed and add the patient tissue to the bottom and then put the slides in the oven (we use 70C for one hour) prior to IHC staining. The tissue lifting decreased dramatically- even on breast tissue. Be sure to pick up your controls from the wrong (label) end when picking up your controls and do not let the bottom half of the slide get into the waterbath so as to avoid double-dipping which can also cause tissue adherence problems. Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Renee H. Workman Sent: Tuesday, February 24, 2015 1:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] sections not sticking to charged slides Help, sections not sticking to charged slides. We use Mercedes Medical charged slides. Lately have been using sta-on but still have occasional problems especially during antigen retrieval. I need any suggestions. Renee H. Workman Histology Supervisor Virginia Urology 9105 Stony Point Drive Richmond, VA 23235 W: 804-527-1316 | F: 804-270-0917 rhwork...@uro.com | www.uro.com Disclaimer: The email and files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the original recipient or the person responsible for the delivering the email to the intended recipient, be advised that you have received this email in error, and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you received this email in error, please delete it from your system without copying it, and notify the sender by reply email so that our address record can be corrected. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] gelatin
Dear ynw...@u.washington.edu. Boiling makes steam, with bubbles that greatly change tissue structure. Slow freezing is just as bad; the ice crystals make holes that deform and replace the tissue architecture. What are you tryng to find out? It has been known for 100+ years that boiling collagen makes gelatin, and further concentration makes traditional glue. You should involve your boss in future email exchanges. John Kiernan London, Canada = = = On 24/02/15, Yak-Nam Wang ynw...@u.washington.edu wrote: Thank you for your e-mail. Apologies for not explaining treated tissue. We treat the tissue with high intensity focused ultrasound. It can raise the temperature of tissue to boiling in a localized area (millimeter areas). I could use a biochemical assay for collagen and gelatin if we treat a large area, but with single lesions I was hoping I could visualize this. In some treated areas we are almost resulting in liquefaction of the tissue. I am interested to see if we are turning the collagen to gelatin in these areas and what part of the lesion this is happening. You should involve your boss in future email exchanges. Thank you for your thoughts Yak-Nam On Mon, Feb 23, 2015 at 9:47 PM, John Kiernan jkier...@uwo.ca wrote: You need to explain treated tissue. Gelatin is collagen that has been boiled until the protein has lost all its fibrous nature and changed into a water-soluble protein. Gelatin is made permanently insoluble by adequate formaldehyde fixation. It is stained by anionic dyes (such as eosin in the HE method), but it does not show as fibres when you look at the section or smear through a microscope. If this doesn't answer your question, please explain your problem and involve your boss in future email exchanges. John Kiernan London, Canada = = = On 23/02/15, Yak-Nam Wang ynw...@u.washington.edu wrote: Hello, Does anyone know of a stain specific for gelatin? I would like to distinguish between firbous collagen and gelatin in treated tissue. thank you Yak-Nam University of Washington Seattle, WA ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: slide folders for double wide slides
Hi Bonnie, we use some from Brain Research laboratories. They are cardboard flats without the dividers that are in the 20-slide types . They have 2x3 slide mailers as well. http://brainresearchlab.com/product-category/slide-storage/slide-folders/ Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Whitaker, Bonnie Sent: Tuesday, February 24, 2015 1:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] slide folders for double wide slides Hi All, What slide folders do any of you recommend for use with 2x3 slides (we use them for whole-mount prostates)? If you have some that work well, could you send me the ordering info? Thanks, Bonnie Bonnie Whitaker AP Operations Director The Ohio State University Wexner Medical Center Department of Pathology N305 Doan Hall 410 West 10th Avenue Columbus, Ohio 43210 614.293.8418 FAX 614.293.2779 Pager: 614.293.7243 ext. 5013 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Prostate Biopsies
Short of using a bar-coded tracking system, does anyone use color-coded formalin containers or cassettes for prostate biopsies (for the urology office staff) to confirm the patient's identity? Thanks. Richard Richard W. Cartun, MS, PhD Director, Histology Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (860) 545-2204 Fax This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
