[Histonet] Tomorrow Tuesday March 10 is Histotechnology Professionals Day!
Hi Histonetters!!! Did you know that tomorrow Tuesday, March 10 is Histotechnology Professionals Day? The National Society for Histotechnology has some great suggestions for ways to celebrate. Here is the link: http://www.nsh.org/content/histotechnology-professionals-day What are you doing to celebrate? I started last week by contacting each and every one of my 1000+ client/employers to let them know that March 10 is the 6th Annual Histotechnology Professionals Day. Hopefully, if they weren't already doing something to show their appreciation they are now!! In honor of Histotechnology Professionals Day. RELIA Solutions celebrates 3 years as Sustaining member of the National Society of Histotechnology. And on Friday I will be lecturing to a group of soon to be histology school graduates on interviewing and job hunting! As always I have amazing opportunities with outstanding clients that I want to tell you about- here is a quick list of the positions I am most excited about. All of these are full time permanent opportunities and our clients offer excellent compensation, benefits and in most cases relo/sign on bonuses. Here is the list of my HOTTEST OPPORTUNITIES !! Histology Leadership Opportunities: Histology Supervisor - Flagstaff, AZ AP Manager - Chicago, IL Histo Supervisor Long Island, NY; (NY license NOT required) Supervisors also needed in MA, MO, and IL Histology Technician/Technologist Histotechnician - Hammond, IN (ASCP OR ELIGIBLE) ***Dermpath Histotech Milwaukee BRAND NEW LAB RELIA EXCLUSIVE!!*** Dermpath Histotech - Kansas, City, KS (ENTRY LEVEL OR EXPERIENCED) Histotechs also needed in TN, MA, NY, OH and VA Maybe it is a good time to reflect on your career, where you are, what you want, where you would like to be and how are you going to get there? If you aren't considering a job change: Is it time to join/renew your NSH membership? Check out your state society? Join the Histonet? Take some CEUs to stay up to date and learn some new skills? Get that state license for the place you want to move to one day? Start studying for that QIHC? If you are or might be considering a job change now or in the near future: Does your resume need to be updated? Have you considered what you are looking for and where? Have you gotten the state license if it is required before you go? Have we spoken recently? Please feel free to contact me by phone toll free at 866-607-3542 or email: rel...@earthlink.net if you want information or help with any of the items listed. My services are always free of charge to you. If you have any friends that might benefit from receiving this please feel free to pass it along. Have A Happy Histo Day!! Pam Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com http://www.facebook.com/PamBarkerRELIA /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
AW: [Histonet] FW: Masson's trichrome stain
Maybe I am wrong, but I think the usage of plastic forceps for trichrome stains is nonsense. I only know this from silver stains, but also have never seen problems personally. The polyacids have to stay onto the section, when anilinblue is added. There exists a theory, that PMA/PTA acts like a mordant and builds bridges between collagen and anilinblue. It is proven, that PMA is not washed out of the tissue and is bound to the fibers. On the other hand it seems, that the polyacids prevent the staining of globular proteins by anilinblue (the longer the polyacids the brighter the red colour). It also helps to keep the low staining-pH of anilinblue. Gudrun Lang From: Justine Lanzon [mailto:justinelan...@hotmail.com] Sent: Thursday, March 05, 2015 5:36 AM To: lindamarg...@gmail.com Subject: Masson's trichrome stain Hi, I am doing a write up on Masson's trichrome stain however I cannot answer these two questions: - Why are plastic forceps used instead of metal ones to hold the stained slide? - Why do we not rinse before Alinine blue? Can you please help me? Many Thanks, Justine Lanzon ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Old slides.
Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Old slides.
Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com mailto:jmcgo...@clinlab.com www.clinlab.com http://www.clinlab.com -Original message- From:Bernice Frederick b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu Sent: Monday, March 9, 2015 1:51 PM To: histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu mailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Mushrooms for GMS fungus control
Try this article... Acta Cytol. 2003 Nov-Dec;47(6):1043-4. Alternative, cost-effective fungus-staining method for control slides in cytology and histopathology. da Silva VD1. Author information Abstract OBJECTIVE: To develop a cost-effective, reliable and safe method of providing fungal control slides for routine use in pathology laboratories. STUDY DESIGN: A set of easily available, low-cost material was tested to obtain fungal colonies on substrate adequate for paraffin-embedded sections or smears. RESULTS: Such material as cheese is a simple, inexpensive and practical culture medium for silver-positive fungi. A batch of paraffin blocks can be prepared to maintain a stock of control material in the laboratory. CONCLUSION: It is useful to maintain fungal colonies to produce staining control specimens using small pieces of refrigerated cheese to easily produce silver-staining control specimens or smears embedded in paraffin, reducing the risk of accidental exposure to potentially infective pathogens in the laboratory. This method might also be a good alternative for conserving routine surgical specimens, considering the currently decreasing numbers of necropsy and large specimens, particularly from immunosuppressed and infected patients. PMID: 14674076 [PubMed - indexed for MEDLINE] -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of koelli...@comcast.net Sent: Sunday, March 08, 2015 4:29 PM To: Linda Prasad (SCHN) Cc: Jeffrey Robinson; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Mushrooms for GMS fungus control Apparently there are numerous interesting ways for fungus or bacteria controls to be had from orange peels to hamburger to slim Jim's to hot dogs to strawberries to . Sounds like fun to me. I'm curious, with the emphasis now on quality control in labs run amok, has anyone passed a rigorous inspection actually showing these as your currently in-use controls? A PI in research who doesn't want his paper rejected at peer review. A CAP inspector in clinical labs who is nit-picky reviewing staining controls but might be looking for a phase anything deficiency. The dot-your-i's and cross-your-t's FDA people who might or might not OK your drug in development. Really, just curious if anyone with a hammer over your head has said it is perfectly fine to use them. Ray, Seattle, WA - Original Message - From: Linda Prasad (SCHN) linda.pra...@health.nsw.gov.au To: Jeffrey Robinson jrobin...@pathology-associates.com, histonet@lists.utsouthwestern.edu Sent: Sunday, March 8, 2015 4:09:02 PM Subject: [Histonet] RE: Mushrooms for GMS fungus control I used strawberries for a fungal control. Worked really good. Linda Prasad | Senior Scientist | Histopathology t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.pra...@health.nsw.gov.au | w: www.schn.health.nsw.gov.au Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia ♲ Please consider the environment before printing this email. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson Sent: Saturday, 7 March 2015 4:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mushrooms for GMS fungus control How about mushrooms? Has anyone had any success using mushrooms as a GMS fungus control? Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses.
Re: [Histonet] FW: Masson's trichrome stain
The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? John Kiernan = = = On 08/03/15, Linda Margraf lindamarg...@gmail.com wrote: Here is a message from Justine... From: Justine Lanzon [mailto:justinelan...@hotmail.com] justinelan...@hotmail.com] Sent: Thursday, March 05, 2015 5:36 AM To: lindamarg...@gmail.com Subject: Masson's trichrome stain Hi, I am doing a write up on Masson's trichrome stain however I cannot answer these two questions: - Why are plastic forceps used instead of metal ones to hold the stained slide? - Why do we not rinse before Alinine blue? Can you please help me? Many Thanks, Justine Lanzon ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Old slides.
Have you done this? Acetone does not dissolve resinous mounting media and allow removal of coverslips. It's all in the books; buy one. John Kiernan Anatomy Cell Biology, UWO London, Canada = = = On 09/03/15, Jason McGough jmcgo...@clinlab.com wrote: Remove the film coverslip by placing the slide in acetone for a few minutes. Then recoverslip the slide with your current method. Jason McGough, HT(ASCP) Operations Manager Clinical Laboratory of the Black Hills 605-343-2267 jmcgo...@clinlab.com mailto:jmcgo...@clinlab.com jmcgo...@clinlab.com www.clinlab.com http://www.clinlab.com -Original message- From:Bernice Frederick b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu b-freder...@northwestern.edu Sent: Monday, March 9, 2015 1:51 PM To: histonet@lists.utsouthwestern.edu mailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu b-freder...@northwestern.edu mailto:b-freder...@northwestern.edu b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu mailto:Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
