Re: [Histonet] Old slides.
Hi We re-Coverslipper a number of sections which had peeled off on the tape as the tape dried and curled. We cut off excess tape using scissors; placed a fresh coverslip flat; put a streak of mounting medium on he coverslip; use a forceps to orientate the tissue + margin of tape; number a slide, dip it in Xylene place on a slope bring on top of coverslip-section-mounting medium; turn the slide-section- coverslip to face coverslip up; leave horizontal to dry (eg overnight). Worth a try, doing one first. Bernie, St Vincent's, Dublin, Ireland On 9 Mar 2015, at 19:41, Bernice Frederick b-freder...@northwestern.edu wrote: Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Old slides.
So we actually cut the film around the section and mount to another slide and do pretty much what wascabove mentioned placing upside downband placing on paper towel. Actually works pretty good. TJUH Sue Paturzo Sent from my Verizon Wireless 4G LTE smartphone Original message From: Gowan,Christie C christiecgo...@dermatology.med.ufl.edu Date:03/09/2015 4:01 PM (GMT-05:00) To: 'Bernice Frederick' b-freder...@northwestern.edu, histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Old slides. Hi Bernice, I have found that if you flood the slide with mounting media (don't use xylene) flip the slide over onto an absorbent lab wipe and put a heavy weight with even pressure and leave for a few hours. If the slide sticks to the wipe just put a few drops of xylene to clean up the slide. You may still have some tiny bubbles but it is much better than the alternative. Good luck. Christie Gowan -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, March 09, 2015 3:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] anti-BRAF V600E (VE1) Ventana
What vendor are you guys using for antibody anti-BRAF V600E (VE1)? Spring Bioscience or Ventana? What dilution are you using? Are you utilizing amplification? Expensive ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PRN
Hi Histonetters, I am looking to fill a PRN Histotech position in the Phoenix area. Does anyone know anyone who is retired and looking for PRN Histology work? Please feel free to e-mail me at the following kcasti...@swskin.netmailto:kcasti...@swskin.net Thank you! This transmission may contain confidential information, some or all of which may be protected health information as defined by the federal Health Insurance Portability Accountability Act (HIPAA) Privacy Rule. This transmission is intended for the exclusive use of the individual or entity to whom it is addressed and may contain information that is proprietary, privileged, confidential and/or exempt from disclosure under applicable law. If you are not the intended recipient (or an employee or agent responsible for delivering this transmission to the intended recipient), you are hereby notified that any disclosure, dissemination, distribution or copying of this information is strictly prohibited and may be subject to legal restriction or sanction. Please notify the sender by telephone to arrange the return or destruction of the information and all copies. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] BIG day today!
That's right, it's OUR day! NSH is doing their thing. Link here: http://www.nsh.org/content/histotechnology-professionals-day . Yours,Dave ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] PA
Can you be a pathology assistant with on the job training only? Does it matter if you have a bachelors vs a masters degree in a science not related to pathology or a tech license vs no tech license? Thanks Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] PA
To be a PA you would need to have ASCP certification. There is one route to be eligible for certification. To be eligible for this examination category, an applicant must have a minimum of a baccalaureate degree from a regionally accredited college/university, AND successful completion of a NAACLS accredited Pathologists’ Assistant program within the last five years. Most of the NAACLS programs are Masters degrees. From: Mike mikeykk...@live.com To: Histonet@lists.utsouthwestern.edu Histonet@lists.utsouthwestern.edu Date: 03/10/2015 08:49 PM Subject:[Histonet] PA Sent by:histonet-boun...@lists.utsouthwestern.edu Can you be a pathology assistant with on the job training only? Does it matter if you have a bachelors vs a masters degree in a science not related to pathology or a tech license vs no tech license? Thanks Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC / Morphometry Technician wanted in Shenandoah Valley Virginia
Histology Laboratory located in the Shenandoah Valley of Virginia is looking to add to it's team. In this position, you will need a working knowledge of IHC theory and practical IHC experience. The best candidate for the position will oversee immunohistochemical staining as well as perform other histology functions including trimming of specimens, paraffin embedding, microtomy and microscopic QC of slides. Experience with morphometry is preferable. Desirable candidates will possess the following: - HT (ASCP) or QIHC registration preferred - 4 years of Histology experience - 1+ years of immunohistochemistry and/or immunofluorescence experience - Keeps abreast with company's current policies and immunohistochemistry technical updates and procedures - Must be able to work independently and in a team environment Full time employment benefits include subsidized medical and dental insurance, vacation, holiday pay, and 401k after 1 year of employment. Compensation is commensurate with experience. If you are interested in this position, please respond to this post with your resume and cover letter. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Old slides.
Hi Bernice, I have found that if you flood the slide with mounting media (don't use xylene) flip the slide over onto an absorbent lab wipe and put a heavy weight with even pressure and leave for a few hours. If the slide sticks to the wipe just put a few drops of xylene to clean up the slide. You may still have some tiny bubbles but it is much better than the alternative. Good luck. Christie Gowan -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernice Frederick Sent: Monday, March 09, 2015 3:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Old slides. Hi all, We received some old slides (1997-1998) that were coverslipped with film. Sakura I would imagine. The issue here is that the coverslips have come up from the slide and the tissue is adhered to the back of the coverslip. They need to be recovered so they can be evaluated. What do you all recommend? We use the CV5030 for coverslipping. I tried one with xylene and mounting media but there were still a couple of air bubbles in there. Thanks, Bernice Bernice Frederick HTL (ASCP) Senior Research Tech Pathology Core Facility Robert. H. Lurie Cancer Center Northwestern University 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 b-freder...@northwestern.edumailto:b-freder...@northwestern.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Direct Hire Histotech South of Tucson, AZ-New Grads Welcome to Apply!
Hello Histonet, I am in search of a histotech for a permanent position about an hour south of Tucson, AZ. New grads welcome to apply and relocation assistance is offered. Please contact me for more details. Thank you! Melissa Owens Allied Search Partners ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TDP43, PTG poly
We stain TDP43 on both FFPE and frozen sections. The FFPE are done on the Bond, but we can't seem to get as good results with the frozen sections on the Bond. Our neuropathologist still prefers manually stained frozen sections for TDP43 over ones done on the Bond. We would really like to get it working reliably on the Bond. Does anyone have a protocol they would be willing to share for frozen sections on the Bond? Victor University of Washington Medical Center ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Job opportunity
Hello everyone and happy National Histotechnologist Day! We are looking for a Histology Supervisor to lead a great team in Hershey Pa. If you are interested, please follow the link below. Thanks for your time, Theresa Career Website: http://www.pennstatehershey.org/web/humanresources/home/searchjobs The Penn State Milton S. Hershey Medical Center is committed to affirmative action, equal opportunity and the diversity of its workforce. Equal Opportunity Employer - Minorities/Women/Protected Veterans/Disabled. All individuals (including current employees) selected for a position will undergo a background check appropriate for the position's responsibilities Theresa Hunter, MLS(ASCP)cm Interim Assistant Manager AP Cytology/Decedent care/Histology Lead Pathologist Assistant Department of Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center 500 University Drive, Mail Code H160 Hershey, Pa. 17033 Phone (717) 531-0003 Ext. 287181 Fax (717)531-0831 thun...@hmc.psu.edumailto:thun...@hmc.psu.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] anti-BRAF V600E (VE1) Ventana
Spring Bioscience is part of Ventana, Spring had the original clone, and now so does Ventana. We use the spring bioscience concentrated antibody on the Bond III at 1:100 dilution, using the Bond Hi-pH ER2 retrieval for 20 minutes. I know that Ventana sells the RTU version. Remember that from Spring it is RUO, so it is up to you to do the appropriate LDT. The Ventana antibody is an IVD. It is also a pretty pricey antibody from either vendor. Let me know if you need any further help. Patrick Laurie(HT)ASCP QIHC Histology Manager Celligent Diagnostics, LLC 101 East W.T. Harris Blvd | Suite 1212 | Charlotte, NC 28262 Work: 704-970-3300 Cell: 704-266-0869 On Tue, Mar 10, 2015 at 1:15 PM, Susan Foreman sfore...@labpath.com wrote: What vendor are you guys using for antibody anti-BRAF V600E (VE1)? Spring Bioscience or Ventana? What dilution are you using? Are you utilizing amplification? Expensive ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Open histology positions: San Francisco
Happy Histology Day, everyone! I'm looking for techs for my new (to me) lab!! I'm working at CPMC Sutter in San Francisco and we need a couple of permanent hires for our team. First few days it looks like it's gonna be fun - rock'n'roll kinda place where quality and speed have equal bearing. Let me know what you're looking for and let's see if it's a match! Email resumes to me at the email, below. Please feel free to share and THANK YOU! Cheryl Kerry, HT(ASCP) ad...@fullstaff.org ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] soft for microwriter (thermo scientific, Lamb, Shandon)
Hi I am looking for labelling software (or advices) for the carousel microwriter (thermo scientific, Lamb, Shandon = same machine) (LAMB E22.01MWR) The machine is discontinuated. I would like to use serial interface rs232 and barcode scanners. Thanks for help. Richard ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification
Thank you for all the helpful suggestions about this topic. My sample has been in the fixative until it would be decalcified. Thank you again, rui From: r...@leicester.ac.uk To: ru...@hotmail.com Subject: RE: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification Date: Tue, 10 Mar 2015 09:49:00 + Leave it in formalin for as long as possible..good luck -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rui TAHARA Sent: 04 March 2015 23:39 To: gayle.cal...@bresnan.net; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification Thank you for helpful suggestions. I have further questions. Yes, I have a bird head (probably 1 cm X 1 cm ) stored in 70 % ethanol. But i have a similar size bird head fixed in 3.7% formalin for over night and am actually processing the head to store in 70% ethanol since my lab is just ordering the decalcifying solution. I need to decalcify this sample later. But i am wondering if it is better to keep the sample in formalin for a week or so till i get the decalcification solution or i should store it in 70 % ethanol and then fix it for a few days again later? I am afraid that longer fixative time would affect the sample somehow (e.g. the sample become too rigid?) Thank you, rui From: gayle.cal...@bresnan.net To: histonet@lists.utsouthwestern.edu Date: Wed, 4 Mar 2015 16:25:28 -0700 Subject: [Histonet] Re: Bird head stored in 70% alcohol and possible decalcification You wrote: I have an adult bird skull that fixed with formalin and then has been stored in 70% ethanol. I have seen the post that the sample stored in 70% ethanol can be walking back through to series of ethanol to water and can be decalcified if it needs to be. I am wondering if anybody has done this and there is any side effects from decalcification after going through dehydration and rehydration of a sample compared to a general straight forward protocol from decalcification to dehydration? ** ** ** ** I have, in the past, when a weekend arrive, I interrupted acid bone decalcification by removing it from acid decalcifier, a quick water rinse and immersed into 70% alcohol before returning bone to fresh acid decalcifier the next working day. The bones always decalcified without problems but I am sure the decalcification took longer since partially decalcified bone had to rehydrate. I later learned more about dipolar (hope I said that correctly) alcohol slowing and/or stopping ionization of calcium and ceased using 70% alcohol to interrupt acid decalcification. I now use NBF to interrupt decalcification. Interestingly, I learned the alcohol technique from the AFIP bone pathology lab. Alcohol is put into Perenyi's nitric acid decalcifying solutions to slow down or control very rapid nitric acid decalcification. You did not say how big the bird skull was? I suggest immersing the skull back into NBF to let it totally rehydrate for several days (depending on skull size and if the brain is present). I suggest changing NBF if you rehydrate longer than a day. You don't need to go back through an alcohol gradient since many processing schedules have tissue samples going from NBF directly into 70%. If you leave residual alcohol in the bones, the acid decalcification could be slower and hopefully not retarded in any way. It certainly is worth a try. Good luck. Gayle M. Callis HTL/HT/MT(ASCP) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
