[Histonet] Verhoeff's hematoxylin
Hi 1) Our college is trying to save on reagents. Most textbooks say that Verhoeff's working solution is stable only for a few hours. Someone told me that he used to keep the same solution for up to a month. Has any of you tried to reuse Verhoeff's working solution? If so, how long did you keep it? 2) When preparing stock solution B (10% ferric chloride), do you use the anhydrous or the hexahydrate crystals ? We've always used 10g of hexahydrate for 100ml of water and it works fine. But I just realized that maybe we've been doing it wrong for years?!? 3) About stock solution A : Someone told me that older solutions work better than fresh ones. Apparently, solutions that are one year old are the best. What's your opinion on that? Andre Kougioumoutzakis College de Rosemont Envoyé de mon iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] RE: Nuclear Artifact
When I had this occur recently and sporadically, it was a collection issue. Joelle Weaver MAOM, HTL (ASCP) QIHC From: lbla...@digestivespecialists.com To: ro...@labcorp.com; histonet@lists.utsouthwestern.edu Date: Tue, 21 Apr 2015 13:48:07 -0400 CC: Subject: [Histonet] RE: Nuclear Artifact The first place I would look is to what may be happening before they reach me. If it's only one site with an issue, it sounds more like an issue at collection. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roy, Lisa Sent: Tuesday, April 21, 2015 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear Artifact Hi HistoNetters: I have run into quite a problem. My lab currently processes all tissue types from 3 different sites. Recently, we have been getting complaints from one of the sites that the biopsies have a nuclear artifact. It is described as washed out or poor to no nuclear detail. Pictures have been uploaded (Nuclear Artifact). The Medical Director at said site is convinced that a processor error is occurring. Our site is not seeing this on any of our slides. Biopsies from all three sites are processed and embed together. We have done all trouble shooting that we can think of. Leica service has come to inspect our Peloris processor and all areas checked out as functioning properly. The problem is not consistent daily. Seems to be worst toward the end of the week. We have been running the same processing protocol, staining protocol and cutting protocols for years now. This problem has just developed over the last 2 months. Any ideas, no matter how far-fetched, would be greatly appreciated at this point. Lisa Roy, HT(ASCP) Histology Supervisor LabCorp at St. Vincent Hospital 123 Summer St Worcester, MA (508)363-9420 -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: (no subject)
-I think you can use the other tissue controls. Even CDC (Hansen's Disease Center) will send out mycobacterium leprae controls free of charge, and I think they are armadillo. The best control I have ever used for mast cells is canine mast cell tumors. I request them from Vet Schools regularly. Also think of antibodies, aren't most antibodies animal? The separation of the specimens I think is during processing. They should be processed separately from routine human tissues when they are being used for clinical human tests. I have heard of them running on the same processor, just on a different run when the solutions have been changed. Toysha - Message: 10 Date: Mon, 20 Apr 2015 18:50:15 -0400 From: Garrey Faller garr...@gmail.com Subject: Re: [Histonet] (no subject) To: koelli...@comcast.net Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: CAF2sxrVD7o96Wz84nDBR6uxf=93tpqpmueqwog57e4fetcc...@mail.gmail.com Content-Type: text/plain; charset=UTF-8 Here is the CAP checklist requirement: ANP.21450 All histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc. Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this. Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, koelli...@comcast.net wrote: I asked about this in a different vein months ago. Has anyone shown a strawberry or ground meat or slim jim or orange peel as a bacteria/fungus control used for diagnostics to an inspector inspecting the lab and was there any comment from the inspector either positive or negative. Never heard back anything. Ray, Lake Forest Park, WA - Original Message - From: tjfinney2...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Sunday, April 19, 2015 5:24:53 PM Subject: [Histonet] (no subject) GMS controls From my understanding we can't use non human controls on patients. I could be wrong, but you may want to look into it. Happy Connecting. Sent from my Sprint Phone. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Sent from my iPhone On 21 Apr 2015, at 8:51 am, Garrey Faller garr...@gmail.com wrote: Here is the CAP checklist requirement: ANP.21450 All histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc. Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this. Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, koelli...@comcast.net wrote: I asked about this in a different vein months ago. Has anyone shown a strawberry or ground meat or slim jim or orange peel as a bacteria/fungus control used for diagnostics to an inspector inspecting the lab and was there any comment from the inspector either positive or negative. Never heard back anything. Ray, Lake Forest Park, WA - Original Message - From: tjfinney2...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Sunday, April 19, 2015 5:24:53 PM Subject: [Histonet] (no subject) GMS controls From my understanding we can't use non human controls on patients. I could be wrong, but you may want to look into it. -- Message: 14 Date: Tue, 21 Apr 2015 13:49:21 + (UTC) From: koelli...@comcast.net Subject: Re: [Histonet] controls to lengthy off topic To: Garrey Faller garr...@gmail.com Cc: histonet@lists.utsouthwestern.edu Message-ID: 2017966350.7376432.1429624161902.javamail.zim...@comcast.net Content-Type: text/plain; charset=utf-8 Hello Garrey, Curious myself, CAP contact info seems to be greyed out on website unless I officially log in and for now my concerns are with the Washington State Science
[Histonet] RE: Nuclear Artifact
I had this issue a couple of years back. Found out that there was a delay of over an hour from the time the specimen was collected to the time it was being put into fixative. Tom Tom Podawiltz HT (ASCP) AP Section Head LRGHealthcare -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roy, Lisa Sent: Tuesday, April 21, 2015 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear Artifact Hi HistoNetters: I have run into quite a problem. My lab currently processes all tissue types from 3 different sites. Recently, we have been getting complaints from one of the sites that the biopsies have a nuclear artifact. It is described as washed out or poor to no nuclear detail. Pictures have been uploaded (Nuclear Artifact). The Medical Director at said site is convinced that a processor error is occurring. Our site is not seeing this on any of our slides. Biopsies from all three sites are processed and embed together. We have done all trouble shooting that we can think of. Leica service has come to inspect our Peloris processor and all areas checked out as functioning properly. The problem is not consistent daily. Seems to be worst toward the end of the week. We have been running the same processing protocol, staining protocol and cutting protocols for years now. This problem has just developed over the last 2 months. Any ideas, no matter how far-fetched, would be greatly appreciated at this point. Lisa Roy, HT(ASCP) Histology Supervisor LabCorp at St. Vincent Hospital 123 Summer St Worcester, MA (508)363-9420 -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet THIS MESSAGE IS CONFIDENTIAL. This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments. If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Nuclear Artifact
Hi HistoNetters: I have run into quite a problem. My lab currently processes all tissue types from 3 different sites. Recently, we have been getting complaints from one of the sites that the biopsies have a nuclear artifact. It is described as washed out or poor to no nuclear detail. Pictures have been uploaded (Nuclear Artifact). The Medical Director at said site is convinced that a processor error is occurring. Our site is not seeing this on any of our slides. Biopsies from all three sites are processed and embed together. We have done all trouble shooting that we can think of. Leica service has come to inspect our Peloris processor and all areas checked out as functioning properly. The problem is not consistent daily. Seems to be worst toward the end of the week. We have been running the same processing protocol, staining protocol and cutting protocols for years now. This problem has just developed over the last 2 months. Any ideas, no matter how far-fetched, would be greatly appreciated at this point. Lisa Roy, HT(ASCP) Histology Supervisor LabCorp at St. Vincent Hospital 123 Summer St Worcester, MA (508)363-9420 -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: Nuclear Artifact
The first place I would look is to what may be happening before they reach me. If it's only one site with an issue, it sounds more like an issue at collection. Linda -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Roy, Lisa Sent: Tuesday, April 21, 2015 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Nuclear Artifact Hi HistoNetters: I have run into quite a problem. My lab currently processes all tissue types from 3 different sites. Recently, we have been getting complaints from one of the sites that the biopsies have a nuclear artifact. It is described as washed out or poor to no nuclear detail. Pictures have been uploaded (Nuclear Artifact). The Medical Director at said site is convinced that a processor error is occurring. Our site is not seeing this on any of our slides. Biopsies from all three sites are processed and embed together. We have done all trouble shooting that we can think of. Leica service has come to inspect our Peloris processor and all areas checked out as functioning properly. The problem is not consistent daily. Seems to be worst toward the end of the week. We have been running the same processing protocol, staining protocol and cutting protocols for years now. This problem has just developed over the last 2 months. Any ideas, no matter how far-fetched, would be greatly appreciated at this point. Lisa Roy, HT(ASCP) Histology Supervisor LabCorp at St. Vincent Hospital 123 Summer St Worcester, MA (508)363-9420 -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] (no subject)
Hi does any one doing mice tibia histology, we use to fix in formalin for 24 hours and transfer in 2and half weeks in 0.5M EDTA three change and process for 24 hours long protocol in automatic processor. But I am facing problem with bone marrow shrinkage. If any one have idea for decalcification timing and solution can resolved this problem and keep bone marrow intact with bone. Thank you in advance. Regards, Shruti Sent from my iPhone On 21 Apr 2015, at 8:51 am, Garrey Faller garr...@gmail.com wrote: Here is the CAP checklist requirement: ANP.21450 All histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc. Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this. Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, koelli...@comcast.net wrote: I asked about this in a different vein months ago. Has anyone shown a strawberry or ground meat or slim jim or orange peel as a bacteria/fungus control used for diagnostics to an inspector inspecting the lab and was there any comment from the inspector either positive or negative. Never heard back anything. Ray, Lake Forest Park, WA - Original Message - From: tjfinney2...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Sunday, April 19, 2015 5:24:53 PM Subject: [Histonet] (no subject) GMS controls From my understanding we can't use non human controls on patients. I could be wrong, but you may want to look into it. Happy Connecting. Sent from my Sprint Phone. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE Please consider the environment before printing this email. This message and any attachments are intended for the addressee named and may contain legally privileged/confidential/copyright information. If you are not the intended recipient, you should not read, use, disclose, copy or distribute this communication. If you have received this message in error please notify us at once by return email and then delete both messages. We accept no liability for the distribution of viruses or similar in electronic communications. This notice should not be removed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Visual alert for processors
Does anyone know of a way to generate a visual alert (hearing impaired device?) to notify us when our processors finish? They are going to be elsewhere in the building and we need to know when they finish. Baby monitor is out of the question due to privacy issues. Sent from my iPhone IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] controls to lengthy off topic
Hello Garrey, Curious myself, CAP contact info seems to be greyed out on website unless I officially log in and for now my concerns are with the Washington State Science and Engineering Fair for K-12 and golf game. (1) There are at least two phrases in the ANP.21450 which could be parsed out similar to the now famous it depends on what the definition of is is. (2) Fortunate, I had micro groups around who could provide me with species specific Candida or Aspergillus or species and morphological identifiable gram positive or gram negative organisms so when I built the controls with fresh human tissue, as has been described several times on Histonet by others, I knew exactly what I was looking at. (3) It appears there may be tens to hundreds of thousands of molds and what is growing in orange peels or strawberries or cream cheese or bacteria in slim jims would be a total mystery but maybe that is OK. Yet, human pathogen or not? rare or common? stains appropriately or not according to what it REALLY is? I'm not saying the controls are wrong; they might be perfectly fine. I'm just curious if anyone being inspected ever put a stained section of a slim jim on scope in front of a Pathologist from the inspecting agency and what was the reaction if any. Ray in Lake Forest Park, WA - Original Message - From: Garrey Faller garr...@gmail.com To: koelli...@comcast.net Cc: tjfinney2...@gmail.com, histonet@lists.utsouthwestern.edu Sent: Monday, April 20, 2015 3:50:15 PM Subject: Re: [Histonet] (no subject) Here is the CAP checklist requirement: ANP.21450 All histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc. Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this. Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, koelli...@comcast.net wrote: I asked about this in a different vein months ago. Has anyone shown a strawberry or ground meat or slim jim or orange peel as a bacteria/fungus control used for diagnostics to an inspector inspecting the lab and was there any comment from the inspector either positive or negative. Never heard back anything. Ray, Lake Forest Park, WA - Original Message - From: tjfinney2...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Sunday, April 19, 2015 5:24:53 PM Subject: [Histonet] (no subject) GMS controls From my understanding we can't use non human controls on patients. I could be wrong, but you may want to look into it. Happy Connecting. Sent from my Sprint Phone. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Florida Society for Histotechnology Meeting
Good Morning Histonetters! Did you know or may be even care to know that we have only 3 days left to guarantee a hotel room for our 2015 Spring FSH Meeting. Final reservations should be made no later than April 23rd, 2015 to ensure rate and availability. After that date you will have to hope that room prices will not go up. Act now to save money and possible aggravation. Looking forward to seeing you at our meeting! By the way all are welcome from near or far! Meeting Program/agenda http://www.fshgroup.org/wp-content/uploads/2015/03/FSH-2015-Online-Program-revised-6.pdf Hotel online reservation https://reservations.ihotelier.com/crs/g_reservation.cfm?groupID=1251402hotelID=6579 Need to book no later than 4-23-15 Online meeting registration https://www.regonline.com/Register/Checkin.aspx?EventID=1679155lbrd=1rtypeid=380141 Have a great day! Kind Regards! John J Shelley 2014-2016 FSH President ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC technician needed in Modesto, CA.
A growing anatomic pathology laboratory in Modesto, CA is looking to hire a IHC technician for their Histology department. ASCP certification preferred. This is a permanent, full time opportunity offering relocation assistance. Please reach out to me for immediate consideration. Thanks so much! Taylor Rinaldi Nationwide Laboratory Recruiter Prometheus Healthcare Office (301) 693-9057 tay...@prometheushealthcare.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] IHC/SS QA/QC Sheets:
Can someone help guide me on the right direction regarding how to organize daily QC sheets. Currently we have one per case (this is time consuming and I'm on overload of papers). Does anyone have a good solution and are you willing to share? Thank you for your help, Craig Sent from my iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Fwd: mouse tibia histology
Sent from my iPhone Begin forwarded message: From: Shruti Shah s.s...@garvan.org.aumailto:s.s...@garvan.org.au Date: 21 April 2015 7:28:07 pm AEST To: Garrey Faller garr...@gmail.commailto:garr...@gmail.com Cc: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] (no subject) Hi does any one doing mice tibia histology, we use to fix in formalin for 24 hours and transfer in 2and half weeks in 0.5M EDTA three change and process for 24 hours long protocol in automatic processor. But I am facing problem with bone marrow shrinkage. If any one have idea for decalcification timing and solution can resolved this problem and keep bone marrow intact with bone. Thank you in advance. Regards, Shruti Sent from my iPhone On 21 Apr 2015, at 8:51 am, Garrey Faller garr...@gmail.commailto:garr...@gmail.com wrote: Here is the CAP checklist requirement: ANP.21450 All histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc. Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this. Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, koelli...@comcast.netmailto:koelli...@comcast.net wrote: I asked about this in a different vein months ago. Has anyone shown a strawberry or ground meat or slim jim or orange peel as a bacteria/fungus control used for diagnostics to an inspector inspecting the lab and was there any comment from the inspector either positive or negative. Never heard back anything. Ray, Lake Forest Park, WA - Original Message - From: tjfinney2...@gmail.commailto:tjfinney2...@gmail.com To: histonet@lists.utsouthwestern.edumailto:histonet@lists.utsouthwestern.edu Sent: Sunday, April 19, 2015 5:24:53 PM Subject: [Histonet] (no subject) GMS controls From my understanding we can't use non human controls on patients. I could be wrong, but you may want to look into it. Happy Connecting. Sent from my Sprint Phone. ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE Please consider the environment before printing this email. This message and any attachments are intended for the addressee named and may contain legally privileged/confidential/copyright information. If you are not the intended recipient, you should not read, use, disclose, copy or distribute this communication. If you have received this message in error please notify us at once by return email and then delete both messages. We accept no liability for the distribution of viruses or similar in electronic communications. This notice should not be removed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edumailto:Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE Please consider the environment before printing this email. This message and any attachments are intended for the addressee named and may contain legally privileged/confidential/copyright information. If you are not the intended recipient, you should not read, use, disclose, copy or distribute this communication. If you have received this message in error please notify us at once by return email and then delete both messages. We accept no liability for the distribution of viruses or similar in electronic communications. This notice should not be removed. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Verhoeff's hematoxylin
Suggested Answers below 1. several hours is all you will get out of the solution I am surprised there is a method that gives you a month shelf life unless it is a different method eg orcein 2. If it works keep using it 3. Allowing the alcoholic Hx to ripen for at least a few days will make it easier to differentiate the elastic fibres. Regards, Tony From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of André K [ak...@hotmail.com] Sent: Tuesday, 21 April 2015 3:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Verhoeff's hematoxylin Hi 1) Our college is trying to save on reagents. Most textbooks say that Verhoeff's working solution is stable only for a few hours. Someone told me that he used to keep the same solution for up to a month. Has any of you tried to reuse Verhoeff's working solution? If so, how long did you keep it? L 2) When preparing stock solution B (10% ferric chloride), do you use the anhydrous or the hexahydrate crystals ? We've always used 10g of hexahydrate for 100ml of water and it works fine. But I just realized that maybe we've been doing it wrong for years?!? 3) About stock solution A : Someone told me that older solutions work better than fresh ones. Apparently, solutions that are one year old are the best. What's your opinion on that? Andre Kougioumoutzakis College de Rosemont Envoyé de mon iPhone ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] autdialer for Peloris
Can anyone recommend a third party auto dialer to connect to Peloris? Thanks, Angie IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail.___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Nuclear Artifact
We occasionally see this also. Sent from my iPhone On Apr 21, 2015, at 7:59 PM, Sue suetp...@comcast.net wrote: OMG we are experiencing the same issue. At first it was just GI and now we are seeing it on prostate. One pathologist said it looks like the tissue has been cooked. The only issue is we can have two biopsies right next to one another in the basket one looks good and one looks bad. My director also thinks it is the processors. I had Thermo out and they could find nothing. We changed out all the reagents and the biopsies were fine than two days later we had some bad ones. I know in July Fisher had a formalin recall associated to the mixture of buffer, water and formalin. We thought that might be it but it is now almost a year later and all the bad formalin should be gone. The histotechs say the tissue is crunchy and they are right. I am running a test tonight of a small needle biopsy that I made from a colon. I placed it is straight formaldehyde overnight and am processing it on our biopsy cycle tonight. My director also wanted us to only put three levels on our Thermo, but he wanted the middle level to have empty baskets. I stopped that today because I think the other issue is that the poor biopsies may be on the top level and as the reagents are used the level changes, and also due to displacement with the middle level being empty the reagent levels may not reach the top. We just do not have the manpower to inspect every reagent every day, we have 6 processor and it would take a tech all day. We actually take a digital picture when they come out of the processor. I want to check my problems cases tomorrow. We do not use sponges but the only other like was the PA who was wrapping the blue paper very tight around the tissue. I really do not think this is the issue though.. Any other insight would be greatly appreciated. Susan T. Paturzo TJUH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Nuclear Artifact
OMG we are experiencing the same issue. At first it was just GI and now we are seeing it on prostate. One pathologist said it looks like the tissue has been cooked. The only issue is we can have two biopsies right next to one another in the basket one looks good and one looks bad. My director also thinks it is the processors. I had Thermo out and they could find nothing. We changed out all the reagents and the biopsies were fine than two days later we had some bad ones. I know in July Fisher had a formalin recall associated to the mixture of buffer, water and formalin. We thought that might be it but it is now almost a year later and all the bad formalin should be gone. The histotechs say the tissue is crunchy and they are right. I am running a test tonight of a small needle biopsy that I made from a colon. I placed it is straight formaldehyde overnight and am processing it on our biopsy cycle tonight. My director also wanted us to only put three levels on our Thermo, but he wanted the middle level to have empty baskets. I stopped that today because I think the other issue is that the poor biopsies may be on the top level and as the reagents are used the level changes, and also due to displacement with the middle level being empty the reagent levels may not reach the top. We just do not have the manpower to inspect every reagent every day, we have 6 processor and it would take a tech all day. We actually take a digital picture when they come out of the processor. I want to check my problems cases tomorrow. We do not use sponges but the only other like was the PA who was wrapping the blue paper very tight around the tissue. I really do not think this is the issue though.. Any other insight would be greatly appreciated. Susan T. Paturzo TJUH ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] mice tibia histology
Your decal time should be according to your mice age. Four weeks old mouse normally need four changes of EDTA in two weeks time. I think your process time is a bit too long. If your four weeks old mouse tibia, 13 hours from 70% ethanol to last infiltration paraffin should be fine. Your shrinkage is because 1. temperature too high?; 2. too long in xylene? hope this help. Dorothy Hu Hi does any one doing mice tibia histology, we use to fix in formalin for 24 hours and transfer in 2and half weeks in 0.5M EDTA three change and process for 24 hours long protocol in automatic processor. But I am facing problem with bone marrow shrinkage. If any one have idea for decalcification timing and solution can resolved this problem and keep bone marrow intact with bone. Thank you in advance. Regards, Shruti ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: (no subject)
A few years ago we got cited for not having the fungal control be in tissue. The citation was from a HQIP survey we participated in from CAP. We were using a cultured fungal specimen in a cell button at that time. Since then I have collected and shared a number of cases we have seen with fungal infections in feet. Just my 2 cents Cindi Robinson, HT(ASCP) Dunes Medical Laboratories 350 W Anchor Dr Dakota Dunes SD 57049 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] on behalf of Mayer,Toysha N [tnma...@mdanderson.org] Sent: Tuesday, April 21, 2015 2:12 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] RE: (no subject) -I think you can use the other tissue controls. Even CDC (Hansen's Disease Center) will send out mycobacterium leprae controls free of charge, and I think they are armadillo. The best control I have ever used for mast cells is canine mast cell tumors. I request them from Vet Schools regularly. Also think of antibodies, aren't most antibodies animal? The separation of the specimens I think is during processing. They should be processed separately from routine human tissues when they are being used for clinical human tests. I have heard of them running on the same processor, just on a different run when the solutions have been changed. Toysha - Message: 10 Date: Mon, 20 Apr 2015 18:50:15 -0400 From: Garrey Faller garr...@gmail.com Subject: Re: [Histonet] (no subject) To: koelli...@comcast.net Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Message-ID: CAF2sxrVD7o96Wz84nDBR6uxf=93tpqpmueqwog57e4fetcc...@mail.gmail.com Content-Type: text/plain; charset=UTF-8 Here is the CAP checklist requirement: ANP.21450 All histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc. Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this. Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, koelli...@comcast.net wrote: I asked about this in a different vein months ago. Has anyone shown a strawberry or ground meat or slim jim or orange peel as a bacteria/fungus control used for diagnostics to an inspector inspecting the lab and was there any comment from the inspector either positive or negative. Never heard back anything. Ray, Lake Forest Park, WA - Original Message - From: tjfinney2...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Sunday, April 19, 2015 5:24:53 PM Subject: [Histonet] (no subject) GMS controls From my understanding we can't use non human controls on patients. I could be wrong, but you may want to look into it. Happy Connecting. Sent from my Sprint Phone. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Sent from my iPhone On 21 Apr 2015, at 8:51 am, Garrey Faller garr...@gmail.com wrote: Here is the CAP checklist requirement: ANP.21450 All histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. Ray...you should call the CAP and ask for guidance on this. My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc. Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this. Call the CAP for a definitive answer. I am interested in their response. Garrey On Sun, Apr 19, 2015 at 9:06 PM, koelli...@comcast.net wrote: I asked about this in a different vein months ago. Has anyone shown a strawberry or ground meat or slim jim or orange peel as a bacteria/fungus control used for diagnostics to an inspector inspecting the lab and was there any comment from the inspector either positive or negative. Never heard back anything. Ray, Lake Forest Park, WA - Original Message - From: tjfinney2...@gmail.com To: histonet@lists.utsouthwestern.edu Sent: Sunday,
[Histonet] Fwd: FW: IQMH Symposium, Toronto, ON CANADA June 4/5, 2015
*From:* June Shin *Sent:* April-21-15 11:53 AM *To:* 'histonet@lists.utsouthwestern.edu' *Subject:* IQMH Symposium, Toronto, ON CANADA June 4/5, 2015 *The 2015 IQMH Symposium* The demands of personalized medicine are upon us, and it is critical that patient tissue specimens are confidently handled with the utmost of care. In pathology, part of protecting patient safety involves having a reliable mechanism to control and track patient tissue specimens. Exact knowledge of ischemia time, fixation and processing of positively-identified patient specimens are vital in identifying whether a human tissue specimen can be utilized for subsequent biomarkers, molecular/genetic studies. This symposium is of direct interest to those professionally engaged in healthcare and medical research, and who are concerned with the handling of human tissue; in particular, pathology laboratories, tissue banks and research units. There will be interactive and plenary sessions, and an opportunity for networking and industry exhibits. *Theme:* Best practices in pathology: knowing exactly how you handle each human tissue specimen you process *Date:* June 4-5, 2015 *Venue:* Hilton Garden Inn Toronto Airport 3311 Caroga Drive Mississauga, ON L4V 1A3 * Registration* *Registration is online only.* Fee: $350 + HST (no refunds or cancellations) *Register for this event:* https://iqmh.org/Shop/Product-Viewer/slug/IQMH-Symposium-2015 -- This email has been scanned for email related threats and delivered safely by Mimecast. For more information please visit http://www.mimecast.com -- ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
