Re: [Histonet] TRAP Stain Help
Travis, Were your samples stored in alcohol? If so, that can kill the TRAP enzyme. Your protocol is similar to ours. Sarah Mack University of Rochester Medical Center Center for Musculoskeletal Research Histology, Biochemistry, and Molecular Imaging Core 601 Elmwood Avenue Box 665 Rochester, NY 14642 (585)-273-3901 From: histonet-requ...@lists.utsouthwestern.edu [histonet-requ...@lists.utsouthwestern.edu] Sent: Tuesday, May 12, 2015 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 138, Issue 14 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonetd=AwICAgc=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhAr=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVom=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyIs=ojqKLAQtVhO8ixOrdIb13M0riQkWk2GB5BRQSgbZ3_Qe= or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than Re: Contents of Histonet digest... Today's Topics: 1. anti-human IgG antibody (Krempley, Amanda L) 2. TRAP Stain Help (Wait, Trevor Jordan) 3. Per Diem Positions at UC San Diego Health System (Paula Sicurello) 4. HE Stainer Question (Paula Sicurello) 5. Re: HE Stainer Question (Roy, Lisa) 6. Re: HE Stainer Question (Sanders, Jeanine (CDC/OID/NCEZID)) 7. Re: HE Stainer Question (Simmons, Christopher) 8. Re: HE Stainer Question (Roy, Lisa) 9. RELIA HOT JOB Alert! Lead Histotech needed for Brand New Lab in Dallas/Ft. Worth. A RELIA EXCLUSIVE!!! (Pam Barker) 10. Re: HE Stainer Question (Joelle Weaver) -- Message: 1 Date: Mon, 11 May 2015 19:52:18 + From: Krempley, Amanda L amanda.kremp...@abbvie.com To: HistoNet histonet@lists.utsouthwestern.edu Subject: [Histonet] anti-human IgG antibody Message-ID: 9667996145fe4392bc5c71b3f3a8a...@usaasecsm048.r0018.collaboration.ecs.hp.com Content-Type: text/plain; charset=utf-8 Hello Histoland, Has anyone used an anti-human IgG antibody that does not cross react with Non-human primates, specifically cynomolgus, for IHC? Could you please share the antibody and protocol that you have had success with? Thank you, Amanda -- Message: 2 Date: Mon, 11 May 2015 20:26:14 + From: Wait, Trevor Jordan wa...@livemail.uthscsa.edu To: Histonet@Lists. Edu histonet@lists.utsouthwestern.edu Subject: [Histonet] TRAP Stain Help Message-ID: 1431375976414.44...@livemail.uthscsa.edu Content-Type: text/plain; charset=iso-8859-1 Hey guys, recently I've used the Sigma Aldrich TRAP Stain Kit in order to stain for Osteoclasts in Formalin Fixed Paraffin Embedded bone tissues that are EDTA decalcified. Unfortunately there was no TRAP stain to be found whatsoever on all slides that I stained. I was hoping that someone with some experience with TRAP stain could really help me out! Here are a couple of reasons that I have wondered as to why the TRAP stain might not be visible 1. Staining too long with Hematoxylin counterstain? I have noticed in several trial runs that sometimes if the Hematoxylin counterstain is too long then this can effect the amount of TRAP stain that shows up with the osteoclasts. Perhaps I'm just over analyzing 2. Perhaps no osteoclasts were present to even staindoes anyone know what the usual ratio of Osteoclasts to Osteocytes are? In the past stains that I have usedwhenever there is an osteoclasts that shows up...it is usually pretty spotty and many times they are in groups together...is this how it normally is? 3. Perhaps the TRAP stain is being washed away through the rinsing with dI or dehydration with ethanol/clearing just before mounting. A protocol that I used before the Sigma Aldrich Kit incorporates incubating the slides in 37 celsius water for 1 hour just prior to allowing the slides to sit in the TRAP stain solution (this is also 37 celsius in the same water bath) and letting it sit for 20 minutes. With this stain...there seems to be a consistent showing of osteoclasts but I'm just not sure if all of the osteoclasts are showing up correctly...that is why I moved to the Sigma Aldrich kit to make sure and the results didn't show ANY osteoclasts there! Anyways...I'm just curious if there are any reasons that the TRAP stain is not showing up...I would appreciate any input! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate -- Message: 3 Date: Mon, 11 May 2015 13:57:21 -0700 From: Paula Sicurello
Re: [Histonet] HE STAINER
Ditto! (except we have tape) Michael Ann On 5/12/15, 11:11 AM, Terri Braud tbr...@holyredeemer.com wrote: Our Sakura Prisma stainer with the Sakura Glas coverslipper has been awesome for over 4 years. Very fast, easy maintenance, very forgiving of tech mistakes (not that we ever make any, lol) I'm not saying that it's better than Leica, just that we've worked it like a dog and have been very happy. Terri Braud Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Holy Redeemer Hospital Laboratory 1648 Huntingdon Pike Meadowbrook, PA 19046 Ph: 215-938-3676 Fax: 215-938-3874 4. HE Stainer Question (Paula Sicurello) Date: Mon, 11 May 2015 14:03:40 -0700 From: Paula Sicurello pat...@gmail.com Subject: [Histonet] HE Stainer Question UCSD is in the market for a new HE stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] TRAP Stain Help
If you include a positive slide to your TRAP? It is a must for each run. It could be many things if you didn't have positive slide, will be hard to troubleshot. Also if you see any pink color befor counterstain/coversliping? If do, there may be because of decolored by ethanol/xylene and mounting media. You have to use water based mounting media. I don't use Sigma kit (tried once), but my protocol is more sensitive. We do trap on both paraffin and MMA sections. If you need I can forward you the paper. Dorothy Hu HSDM Message: 2 Date: Mon, 11 May 2015 20:26:14 + From: Wait, Trevor Jordan wa...@livemail.uthscsa.edu To: Histonet@Lists. Edu histonet@lists.utsouthwestern.edu Subject: [Histonet] TRAP Stain Help Message-ID: 1431375976414.44...@livemail.uthscsa.edu Content-Type: text/plain; charset=iso-8859-1 Hey guys, recently I've used the Sigma Aldrich TRAP Stain Kit in order to stain for Osteoclasts in Formalin Fixed Paraffin Embedded bone tissues that are EDTA decalcified. Unfortunately there was no TRAP stain to be found whatsoever on all slides that I stained. I was hoping that someone with some experience with TRAP stain could really help me out! Here are a couple of reasons that I have wondered as to why the TRAP stain might not be visible 1. Staining too long with Hematoxylin counterstain? I have noticed in several trial runs that sometimes if the Hematoxylin counterstain is too long then this can effect the amount of TRAP stain that shows up with the osteoclasts. Perhaps I'm just over analyzing 2. Perhaps no osteoclasts were present to even staindoes anyone know what the usual ratio of Osteoclasts to Osteocytes are? In the past stains that I have usedwhenever there is an osteoclasts that shows up...it is usually pretty spotty and many times they are in groups together...is this how it normally is? 3. Perhaps the TRAP stain is being washed away through the rinsing with dI or dehydration with ethanol/clearing just before mounting. A protocol that I used before the Sigma Aldrich Kit incorporates incubating the slides in 37 celsius water for 1 hour just prior to allowing the slides to sit in the TRAP stain solution (this is also 37 celsius in the same water bath) and letting it sit for 20 minutes. With this stain...there seems to be a consistent showing of osteoclasts but I'm just not sure if all of the osteoclasts are showing up correctly...that is why I moved to the Sigma Aldrich kit to make sure and the results didn't show ANY osteoclasts there! Anyways...I'm just curious if there are any reasons that the TRAP stain is not showing up...I would appreciate any input! Trevor Jordan Wait University of Texas Health Science Center, San Antonio Class of 2017 MD Candidate ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] : Re: HE Stainer Question
We just got rid of our Leica XL. It was not working right after about 10 years, little things started going wrong. And then it just quit reading the white clip that we used for counterstaining IHC. The cost for someone to come to the lab to fix it was ridiculous. The customer service from Leica was not the best. We now have a Gemini from Thermo and love it. It has two ovens and stains similar to the Leica. So far we have not had any problems and we LOVE our Thermo/Fisher reps. They have been wonderful. Kathy Machado, HTL Danville Regional Medical Center Danville, VA kathy.mach...@lpnt.netmailto:kathy.mach...@lpnt.net 434-799-3867 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] assigning pathologists cases for satellite labs
Hello out in Histoland! I have questions regarding the triaging of case slides for delivery to satellite facilities where pathologists will interpret slides. Our LIS system is available at all sites for ordering deepers/special stains/IHC, etc. so we have that covered. We currently do not have digital pathology (yet!) so slides must be delivered to several hospital sites where pathologists are working. Can you all help with thoughts regarding pre-assigning pathologists to cases so that everyone in the lab knows where those slides are supposed to go? We've never assigned pathologists before, it was always first come, first served. How do you all deliver (in a timely fashion) cases to satellite facilities and determine who gets what for general work vs specialists (dermatopathologists)? It gets pretty chaotic back in our lab trying to figure out who gets what and where are they today. . . Oh, and they all want the slides by 7 a.m. ;) Thank you in advance for the advice- Michael Ann Michael Ann Jones, HT (ASCP) Histology Manager Metropath 7444 W. Alaska Dr. #250 Lakewood, CO 80226 303.634.2511 mjo...@metropath.com ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE Stainer Question
Personally I love the Prisma for volume and the tape. I know many have bad opinions, but I wish I had both the Prisma and the tape right now! I have never seen any problems with very old ( 15+ year) slides. using the tape. Not saying it can't happen-but have not personally seen it. The tape is easier to get off if you need to versus old glass CS, just use acetone, acetone/xylene, xylene. Comes off in a gel form and slides right off leaving the tissue intact. Joelle Weaver MAOM, HTL (ASCP) QIHC From: ro...@labcorp.com To: pat...@gmail.com; histonet@lists.utsouthwestern.edu Date: Tue, 12 May 2015 12:18:46 + Subject: Re: [Histonet] HE Stainer Question Paula Here are my two cents I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes of f with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. Hope it helpsgood luck. Lisa -Original Message- From: Paula Sicurello [mailto:pat...@gmail.com] Sent: Monday, May 11, 2015 5:04 PM To: HistoNet Subject: [Histonet] HE Stainer Question Me again... UCSD is in the market for a new HE stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE Stainer Question
Look at all the automated HE stain instruments on the market. I suggest that you consider those that offer the best benefit to your workload and workflow. Instruments that utilize the concept of co-location (related tasks linked together; oven fro drying, flexible stain configuration and Coverslipping) will assist you in developing a LEAN workflow. Consider how many times you need to touch the slides to complete all the tasks and how much walk away time you gain. I do not suggest by-passing the validated staining instrument oven. Placing slides in another oven creates variation and often results in short and extended drying times. All automated HE stain instruments should be used according to manufacturer recommendation. My experience is that when shortcuts are used, quality suffers. The automated HE stain instruments have great through put and you should adjust workflow to maximize the designated batch size and stain time. Film coverslip lasts longer than 7 years. Sakura film is the best and has testing to exceed 10 years. I have used it for 13 years and never had the film peel. If you use the knock off film products, they only have short term accelerated stability testing and probably do not have any version of their many changes to the emulsion that have real time stability testing to exceed 10 years. There are reasons to use glass and reasons to use film. Both are great products, when you use them correctly and purchase quality products. Glass automated cover slip options on instruments do have more required maintenance than film. CAP has made no statement about film cover slipping. In fact, the Hologic (was Cytech) Cytology Thinprep system is FDA approved with film as the cover slip and the stained and film cover slipped Thinprep slide is digitally scanned for analysis. To be CAP compliant, you must keep blocks and slides for 10 years. With the advancement of cancer hospital protocols and molecular testing, many institutions are considering retaining blocks and slides beyond 10 years. Keeping blocks and slides longer than regulation requires introduces a large risk factor for the retaining institution and pathologists. Always be forward thinking when considering the purchase of a new essential instrument. Will it bar code read, can it be interfaced to LIS or tracking system, what analytics can be extract and will the instrument help or hinder a LEAN workflow? There are many choices that will meet your basic needs, but which one meets your essential needs? William DeSalvo, BS HTL(ASCP) From: sim...@upmc.edu To: ro...@labcorp.com; pat...@gmail.com; histonet@lists.utsouthwestern.edu Date: Tue, 12 May 2015 12:32:43 + Subject: Re: [Histonet] HE Stainer Question To be fair, a batch of slides for Leica is actually 270- slides, It can run 9 racks at a time, but, the 9th rack from start to coverslip is 3 hours+ You can always skip the on-board oven and place your slides in a slide dryer (most labs have them) and then every 3 minutes you can load a rack (1st xylene step 3 minutes) Then it goes much faster. As for tape..ugh..it is only guaranteed to last 7 years, after that they pull off the slide and take the tissue with it. CAP is starting to frown on this as you need to keep the initial HE slides for up to 10+ years. Chris Simmons B.S., A.S., HTL(ASCP) Supervisor, UPP Dermatopathology 412.864.3880 office 412.612.0881 cell -Original Message- From: Roy, Lisa [mailto:ro...@labcorp.com] Sent: Tuesday, May 12, 2015 8:19 AM To: Paula Sicurello; HistoNet Subject: Re: [Histonet] HE Stainer Question Paula Here are my two cents I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.no one likes the film coverslips. One advantage to the film, is that the slides
Re: [Histonet] HE Stainer Question
I agree with William tape from anyone else but Sakura is not worth the time and effort. We were just CAP inspected and they said nothing about tape. I have had issues with tape and usually just cut around the sample on the tape and recover on a slide, works beautifully. As far as the oven time is essential and we do use an outside oven but validated using it. I agree to purchase a Leica with an attached cover slipper better lean approach. Stainers that use proprietary reagents bring costs up and in this day and age hospitals re putting us on a tighter budget. Just my 2 cents S paturzo ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE Stainer Question
This is what we have Leica) and it has 4 ovens. http://www.leica-microsystems.com/news-media/news/news-details/article/leica-st5020-multistainer-workstation/ We also have a Prisma and we like them both. We have had the Leica longer and it just never breaks down.knock on wood! We have to have glass coverslips and the Prisma glass coverslipper is a bit more finicky than the Leica. -Original Message- From: Roy, Lisa [mailto:ro...@labcorp.com] Sent: Tuesday, May 12, 2015 8:19 AM To: Paula Sicurello; HistoNet Subject: Re: [Histonet] HE Stainer Question Paula Here are my two cents I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. Hope it helpsgood luck. Lisa -Original Message- From: Paula Sicurello [mailto:pat...@gmail.com] Sent: Monday, May 11, 2015 5:04 PM To: HistoNet Subject: [Histonet] HE Stainer Question Me again... UCSD is in the market for a new HE stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE Stainer Question
Paula Here are my two cents I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. Hope it helpsgood luck. Lisa -Original Message- From: Paula Sicurello [mailto:pat...@gmail.com] Sent: Monday, May 11, 2015 5:04 PM To: HistoNet Subject: [Histonet] HE Stainer Question Me again... UCSD is in the market for a new HE stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE Stainer Question
To be fair, a batch of slides for Leica is actually 270- slides, It can run 9 racks at a time, but, the 9th rack from start to coverslip is 3 hours+ You can always skip the on-board oven and place your slides in a slide dryer (most labs have them) and then every 3 minutes you can load a rack (1st xylene step 3 minutes) Then it goes much faster. As for tape..ugh..it is only guaranteed to last 7 years, after that they pull off the slide and take the tissue with it. CAP is starting to frown on this as you need to keep the initial HE slides for up to 10+ years. Chris Simmons B.S., A.S., HTL(ASCP) Supervisor, UPP Dermatopathology 412.864.3880 office 412.612.0881 cell -Original Message- From: Roy, Lisa [mailto:ro...@labcorp.com] Sent: Tuesday, May 12, 2015 8:19 AM To: Paula Sicurello; HistoNet Subject: Re: [Histonet] HE Stainer Question Paula Here are my two cents I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. Hope it helpsgood luck. Lisa -Original Message- From: Paula Sicurello [mailto:pat...@gmail.com] Sent: Monday, May 11, 2015 5:04 PM To: HistoNet Subject: [Histonet] HE Stainer Question Me again... UCSD is in the market for a new HE stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HE Stainer Question
Depends on which model you have...hence XL. Ours only has one oven so you're really only getting 3-4 racks stained at a time. -Original Message- From: Simmons, Christopher [mailto:sim...@upmc.edu] Sent: Tuesday, May 12, 2015 8:33 AM To: Roy, Lisa; Paula Sicurello; HistoNet Subject: RE: [Histonet] HE Stainer Question To be fair, a batch of slides for Leica is actually 270- slides, It can run 9 racks at a time, but, the 9th rack from start to coverslip is 3 hours+ You can always skip the on-board oven and place your slides in a slide dryer (most labs have them) and then every 3 minutes you can load a rack (1st xylene step 3 minutes) Then it goes much faster. As for tape..ugh..it is only guaranteed to last 7 years, after that they pull off the slide and take the tissue with it. CAP is starting to frown on this as you need to keep the initial HE slides for up to 10+ years. Chris Simmons B.S., A.S., HTL(ASCP) Supervisor, UPP Dermatopathology 412.864.3880 office 412.612.0881 cell -Original Message- From: Roy, Lisa [mailto:ro...@labcorp.com] Sent: Tuesday, May 12, 2015 8:19 AM To: Paula Sicurello; HistoNet Subject: Re: [Histonet] HE Stainer Question Paula Here are my two cents I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on. On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results. Hope it helpsgood luck. Lisa -Original Message- From: Paula Sicurello [mailto:pat...@gmail.com] Sent: Monday, May 11, 2015 5:04 PM To: HistoNet Subject: [Histonet] HE Stainer Question Me again... UCSD is in the market for a new HE stainer for our new hospital opening next year. We need a workhorse, not a prima dona, something with a coverslipper built in would be nice. What do you use? Suggestions gratefully accepted-even from you two Keith and Matt ;) Opinions about the good, the bad, and the ugly (as long as it works really well) will be helpful. Thanks oodles! Paula :-) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyoffi...@labcorp.com or call (877) 23-HIPAA / (877) 234-4722.
[Histonet] RELIA HOT JOB Alert! Lead Histotech needed for Brand New Lab in Dallas/Ft. Worth. A RELIA EXCLUSIVE!!!
Hi Histonetters!! How are you? I have an exciting opportunity that just might interest you. OR If you happen to know someone qualified who might be interested I welcome you to refer them. If I place them you will earn a referral fee. The position we have been engaged to work on is an ASCP Certified histotech with dermpath experience (Mohs is a plus and my client will train). This is for a BRAND NEW LAB located in the Dallas/Fort Worth area. This person will be the sole practitioner histotech in this brand new lab. You will be responsible for managing and maintaining the lab and performing histology. My client offers a competitive salary, nice benefits and an outstanding opportunity. For more information please contact me Pam Barker at rel...@earthlink.net or toll free at 866-607-3542. Thanks-Pam Right Place, Right Time, Right Move with RELIA! Thank You! Pam M. Barker Pam Barker President/Senior Recruiting Specialist-Histology RELIA Solutions Specialists in Allied Healthcare Recruiting 5703 Red Bug Lake Road #330 Winter Springs, FL 32708-4969 Phone: (407)657-2027 Cell: (407)353-5070 FAX: (407)678-2788 E-mail: rel...@earthlink.net www.facebook.com http://www.facebook.com/PamBarkerRELIA /PamBarkerRELIA www.linkedin.com/in/reliasolutions www.twitter.com/pamatrelia ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
