Re: [Histonet] Paraffin block disposal
I agree with Brian, but we dispose of blocks by treating them as regulated biohazard waste. We also blocks them longer than 2 years. The CLIA regulation states keeping them for a minimum of 2 years. Outside facilities frequently request unstained slides or blocks on cases that are more than 2 years old. Also, some patients require treatment for conditions for many years after the specimen is taken. If storage is not an issue, keeping blocks 10 years (CAP requirements) is reasonable. Brendal C. Finlay, HT (ASCP) Senior Histologist Medical Center Clinic, P.A 8333 North Davis Highway Pensacola, FL 32514 Phone 850.474.8581 Fax 850.474.8584 -Original Message- From: Cooper, Brian bcoo...@chla.usc.edu To: a.tolentin...@gmail.com Cc: histonet@lists.utsouthwestern.edu Date: 06/06/2015 13:34 Subject: Re: [Histonet] Paraffin block disposal Hey Aimee, This has been discussed several times on Histonet. It sounds like it depends on the institution. Since they're FFPE, pathogens are not a concern. I didn't reply to all because someone will shout out, What about CJD? and then I would have to punch them. They should be able to go into the regular trash though, since there is nothing that anyone can catch from them. Here, just like Genzyme, we are told to dispose of them as regulated, biohazard waste. You would have PHI concerns if the patient's name is on them, so they'll need to be identified first . . . Thanks, Brian Cooper, HT (ASCP) Supervisor, Histology Children's Hospital, Los Angeles Sent from my Galaxy S5, so please forgive any weird typos . . . -Original Message- From: Aimee Tolentino [a.tolentin...@gmail.com] Received: Saturday, 06 Jun 2015, 10:25AM To: Arbaugh, Roberta [rarba...@csdermatology.com] CC: histonet@lists.utsouthwestern.edu [histonet@lists.utsouthwestern.edu] Subject: Re: [Histonet] Paraffin block disposal That's a good question. I'd like to know the answer myself to that. :) Sent from my iPhone On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta rarba...@csdermatology.com wrote: Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them? DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] tissue fixation-formaldehyde concentrations which is best.
John, I totally agree Tony From: John Kiernan [jkier...@uwo.ca] Sent: Saturday, 6 June 2015 2:31 PM To: Peter Noyce; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] tissue fixation-formaldehyde concentrations which is best. Dear Peter, Some of the information you mention as anecdotal is wrong. Formaldehyde and paraformaldehyde are well documented in original peer-reviewed papers and in all textbooks in the fields of histotechnology and histochemistry. Your anecdote about high concentrations of formaldehyde quickly form a 'shell' in the tissue and will stop good penetration and fixation to the deeper tissues has no basis in published work. Paraformaldehyde is an insoluble polymer, not non polymerized formaldehyde. There is no such thing as 4% paraformaldehyde! It is a sad fact that many labs do not contain even one book about histotechnology. Nearly all books in the field (and there are many) have plenty of references to review articles and original literature about the techniques. There are also several websites that provide links to useful papers. Check out some of the useful links on the Biological Stain Commission's site: http://biologicalstaincommission.org/useful-links/ As a graduate student, you need to work from primary sources or reliable secondary sources. When you defend your thesis, you won't want to justify your fixation or staining method by saying I got the method by asking on an internet listserver. John Kiernan Professor Emeritus Anatomy Cell Biology University of Western Ontario London,Canada = = = On 05/06/15, Peter Noyce pwno...@gmail.com wrote: Formaldehyde 37% (commonly called 100% formalin) compared to 4% ( commonly known as 10% neutral buffered formalin)-in theory the 37% should fix quicker and better BUT anecdotally it is said that high concentrations of formaldehyde quickly form a shell in the tissue and will stop good penetration and fixation to the deeper tissues AND over the years it has been said anecdotally that 4% concentration is the quickest and most complete for all sample (mammal and plant) fixation and preservation-are these true. Please do discuss the methanol or buffers that is in the formaldehyde, or discuss paraformaldehyde (which is non polymerized formaldehyde with no methanol, in water). Regards Peter Noyce PhD student. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] [EXTERNAL] Re: plants in the lab
I agree with Paula. The way you have explained the regs implies that even I would have problems entering the labs. Maybe ban people altogether and then we meet the regs. Lets keep it real and not fantasy. Tony From: Boyd, Debbie M [dkb...@chs.net] Sent: Thursday, 4 June 2015 9:22 PM To: Paula Pierce; Goins, Tresa; Histonet Subject: Re: [Histonet] [EXTERNAL] Re: plants in the lab There is a plethora of Joint Commission regulations concerning the below mentioned points of entry for microbacterial spores. If you are Joint Commissioned inspected, plants are not allowed in the lab. We are JC inspected and are not even allow to keep an exterior shipping package. If the box has a shipping label it has to be emptied and placed in plastic tubs. Debbie M. Boyd HT (ASCP) | Chief Histologist | Southside Regional Medical Center | 200 Medical Park Blvd. | Petersburg, Va. 23805 | PH 804-765-5025 | FAX 804-765-6058 From: Paula Pierce [cont...@excaliburpathology.com] Sent: Wednesday, June 03, 2015 11:42 AM To: Goins, Tresa; Histonet Subject: [EXTERNAL] Re: [Histonet] plants in the lab Ugh. TOO MANY REGULATIONS! What about plants and flowers taken to patient's rooms as get well wishes!?! Soil on shoes? Incoming air every time the front doors open an infinite number of times a day? Boxes supplies come in? Have you ever seen inside a semi truck trailer? The multiple holding docks boxes sit on awaiting transport? We cannot live in a bubble. Paula Pierce, BS, HTL(ASCP)HT President Excalibur Pathology, Inc. 5830 N Blue Lake Dr. Norman, OK 73069 405-759-3953 PH 405-759-7513 FAX www.excaliburpathology.com From: Goins, Tresa tgo...@mt.gov To: Tony Henwood (SCHN) tony.henw...@health.nsw.gov.au; Michelle Lamphere michelle.lamph...@childrens.com; 'histonet@lists.utsouthwestern.edu' histonet@lists.utsouthwestern.edu Sent: Wednesday, June 3, 2015 9:18 AM Subject: Re: [Histonet] plants in the lab Patients do not have to go to the fungal spores, the spores will go to the patient. Depending on spore size, the spores may stay airborne for months - the spores sediment to a surface in still air. A condition not likely to occur in a hospital environment - they scurry around until finding a lung or mucous membrane to adhere to. It doesn't take long for a single miss-handled Aspergillus culture plate to contaminate an entire multi-story research lab. -Original Message- From: Tony Henwood (SCHN) [mailto:tony.henw...@health.nsw.gov.au] Sent: Tuesday, June 02, 2015 3:20 PM To: Michelle Lamphere; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Hi Michelle, Why would patients be in a histo lab anyway? From: Michelle Lamphere [michelle.lamph...@childrens.com] Sent: Sunday, 31 May 2015 10:36 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Our hospital Safety and Infection Control departments have policies in place prohibiting any potted plants from being in the hospital, anywhere. We can have them if they are only in water, but the soil presents an infection control issue for patients because of potential mildew, mold, spores, etc. Michelle Lamphere Senior Tech, Histology Anatomic Pathology O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamph...@childrens.com 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 Message: 2 Date: Fri, 29 May 2015 14:23:00 -0400 From: Blazek, Linda lbla...@digestivespecialists.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] plants in the lab Message-ID: 5a2bd13465e061429d6455c8d6b40e391742126...@ibmb7exchange.digestivespecialists.com Content-Type: text/plain; charset=us-ascii Happy Friday all! Does anyone have documentation of the benefit of having plants in the lab? I know this was discusses quite a while ago but I can't find references for it. Any help would be appreciated. Thanks, Linda Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information.
Re: [Histonet] plants in the lab
Laboratory Air-conditioning systems should be separate from patient area systems (as is required for operating theatre units). I understand this to be good practice. From: Goins, Tresa [tgo...@mt.gov] Sent: Thursday, 4 June 2015 12:18 AM To: Tony Henwood (SCHN); Michelle Lamphere; 'histonet@lists.utsouthwestern.edu' Subject: RE: plants in the lab Patients do not have to go to the fungal spores, the spores will go to the patient. Depending on spore size, the spores may stay airborne for months - the spores sediment to a surface in still air. A condition not likely to occur in a hospital environment - they scurry around until finding a lung or mucous membrane to adhere to. It doesn't take long for a single miss-handled Aspergillus culture plate to contaminate an entire multi-story research lab. -Original Message- From: Tony Henwood (SCHN) [mailto:tony.henw...@health.nsw.gov.au] Sent: Tuesday, June 02, 2015 3:20 PM To: Michelle Lamphere; 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Hi Michelle, Why would patients be in a histo lab anyway? From: Michelle Lamphere [michelle.lamph...@childrens.com] Sent: Sunday, 31 May 2015 10:36 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] plants in the lab Our hospital Safety and Infection Control departments have policies in place prohibiting any potted plants from being in the hospital, anywhere. We can have them if they are only in water, but the soil presents an infection control issue for patients because of potential mildew, mold, spores, etc. Michelle Lamphere Senior Tech, Histology Anatomic Pathology O: 214.456.2318 | Fax: 214.456.0779 E: michelle.lamph...@childrens.com 1935 Medical District Drive | B1.06 | Dallas, Texas 75235 Message: 2 Date: Fri, 29 May 2015 14:23:00 -0400 From: Blazek, Linda lbla...@digestivespecialists.com To: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Subject: [Histonet] plants in the lab Message-ID: 5a2bd13465e061429d6455c8d6b40e391742126...@ibmb7exchange.digestivespecialists.com Content-Type: text/plain; charset=us-ascii Happy Friday all! Does anyone have documentation of the benefit of having plants in the lab? I know this was discusses quite a while ago but I can't find references for it. Any help would be appreciated. Thanks, Linda Please consider the environment before printing this e-mail This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses. * ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet * This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network. This note also confirms that
[Histonet] Pre-floating tissue sections in dilute alcohol
Hi everyone, I just became aware of this technique last week, and it seems to work great. I did a quick google search and found this quick reference. http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath? Its an extra step and introduces an extra chance to introduce floaters. But, the quality seems to be improved. Any thoughts? Thanks in advance. Garrey Faller Pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Pre-floating tissue sections in dilute alcohol
Yes I use it for brain. Happy Connecting. Sent from my Sprint Phone. -- Original message-- From: Garrey Faller Date: Sat, Jun 6, 2015 10:48 AM To: histonet@lists.utsouthwestern.edu; Subject:[Histonet] Pre-floating tissue sections in dilute alcohol Hi everyone, I just became aware of this technique last week, and it seems to work great. I did a quick google search and found this quick reference. http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath? Its an extra step and introduces an extra chance to introduce floaters. But, the quality seems to be improved. Any thoughts? Thanks in advance. Garrey Faller Pathologist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Decalcification of bone marrows
Adrienne (where?) asks: I have a really quick question: about how long does it take to decal a bone marrow biopsy? to which Jessica (where? - apparently in the US though) replies It all depends on what you use for decal. We use 5% Nitric acid for 1 hour or so. Sometimes it needs a bit more time. To decalcify a Jamshidi needle bone marrow biopsy specimen in one of the ordinary commercial decalcifiers (usually hydrochloric acid, all deep dark trade secrets) takes about two hours. Nitric acid is NOT an acceptable decalcifier today, since it destroys immunoreactivity. I used to use it for most decalcification in the days before immunohistochemistry, but remember I've been practicing pathology for more than fifty years. Successful processing of bone marrow biopsy specimens requires cooperation among hematologist-oncologists, pathologists, and histotechnologists. (Dream on!) Practically speaking, you have to set a deadline - if your specimen arrives after 2 PM (for example) it doesn't get processed until tomorrow. Once again - Decal is the registered trademark of Decal Chemical Corporation's proprietary decalcifier. It is not a generic word for decalcifiers. (No, I don't work for them.) Bob Richmond Samurai Pathologist Maryville, TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Pre-floating tissue sections in dilute alcohol
I Garrey, I came across floating sections in dilute alcohol back in 2005 when I started working in the UK and have been using it ever since. I agree it introduces one more step and wouldn't use for every single section - there's no need. But I do find it very helpful with certain blocks/tissues - especially the very 'wrinkly' ones. When I started cutting during my training, we used other technique to avoid wrinkles (and waste of tissue sections) and produce 'perfect' sections. We used to have a small cold water bath to float and pick up the sections before transferring to the temperature controlled water bath. Joana Joana Moreira Supervisor - Anatomical Pathology Department of Pathology Sidra Medical Research Center PO Box 26999 | Doha, Qatar Direct Line +974-4404-2036 jmore...@sidra.org | www.sidra.org Message: 12 Date: Sat, 6 Jun 2015 10:29:03 -0400 From: Garrey Faller garr...@gmail.com To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pre-floating tissue sections in dilute alcohol Message-ID: a7285ef9-fa0f-4121-aa4c-78dfa5f1c...@gmail.com Content-Type: text/plain; charset=us-ascii Hi everyone, I just became aware of this technique last week, and it seems to work great. I did a quick google search and found this quick reference. http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath? Its an extra step and introduces an extra chance to introduce floaters. But, the quality seems to be improved. Any thoughts? Thanks in advance. Garrey Faller Pathologist -- Subject: Digest Footer ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- End of Histonet Digest, Vol 139, Issue 6 Disclaimer: This email and its attachments may be confidential and are intended solely for the use of the individual to whom it is addressed. If you are not the intended recipient, any reading, printing, storage, disclosure, copying or any other action taken in respect of this e-mail is prohibited and may be unlawful. If you are not the intended recipient, please notify the sender immediately by using the reply function and then permanently delete what you have received. Any views or opinions expressed are solely those of the author and do not necessarily represent those of Sidra Medical and Research Center. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] tissue fixation-formaldehyde concentrations
I have to agree with the student, John. Sure, he is coming from ignorance ( not a bad situation: naivety is not a fault... we all are/were there at some point;-) Sure...I agree with you re the using of the word Paraformaldehyde as a fixative I often sigh when used. However, differential fixation ( zonal fixation) has always been an issue. We often see the zonal effects of this? Particularly in lymph nodes ( parenchymatous tissues) Obviously, when immersion fixing. Most often ...NOT with agitation. Respectfully, Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6813 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin block disposal
That's a good question. I'd like to know the answer myself to that. :) Sent from my iPhone On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta rarba...@csdermatology.com wrote: Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them? DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Paraffin block disposal
Hey Aimee, This has been discussed several times on Histonet. It sounds like it depends on the institution. Since they're FFPE, pathogens are not a concern. I didn't reply to all because someone will shout out, What about CJD? and then I would have to punch them. They should be able to go into the regular trash though, since there is nothing that anyone can catch from them. Here, just like Genzyme, we are told to dispose of them as regulated, biohazard waste. You would have PHI concerns if the patient's name is on them, so they'll need to be identified first . . . Thanks, Brian Cooper, HT (ASCP) Supervisor, Histology Children's Hospital, Los Angeles Sent from my Galaxy S5, so please forgive any weird typos . . . -Original Message- From: Aimee Tolentino [a.tolentin...@gmail.com] Received: Saturday, 06 Jun 2015, 10:25AM To: Arbaugh, Roberta [rarba...@csdermatology.com] CC: histonet@lists.utsouthwestern.edu [histonet@lists.utsouthwestern.edu] Subject: Re: [Histonet] Paraffin block disposal That's a good question. I'd like to know the answer myself to that. :) Sent from my iPhone On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta rarba...@csdermatology.com wrote: Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them? DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet - CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or legally privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of this original message. - ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
