[Histonet] Missouri Society for Histotechnology Lunch and Learn

[Kelley, Amanda via Histonet]

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[Histonet] axolotl lymphatics and Okada article

[Jason Palmer via Histonet]

Hi all, 

A few weeks back I posted a question about identification of lymphatics in the 
axolotl (see below). John Kiernan suggested that enzyme histochem on paraffin 
sections might be possible, as per an article by Okada et al in 1994. I have 
been trying to hunt down this article but so far to no avail. Does anyone have 
a copy they may be able to scan and send me? It is in a procedings supplement, 
Lymphology journal: 

Okada, E. (1994). An improved enzyme-histochemical method for identification of 
lymphatic capillaries on paraffin sections. Lymphology 27 , Suppl:732-735. 

Book title is: 

Progress in Lymphology XIV—Proceedings of the XIVth Congress in Washington 
DC, 1993, MH Witte, CL Witte (Eds.): Lymphology 27(Suppl): 1-893, 1994. 

Many thanks , 

Jason 

-- 

Jason Palmer 
Histology Laboratory Coordinator 
O'Brien Institute / St Vincent's Institute 
42 Fitzroy St, Fitzroy Victoria 3065 
Australia 
tel +61 3 9288 4045 
fax +61 3 9416 0926 
email: jpal...@svi.edu.au 
- Original Message -
From: "John Kiernan"  
To: "Jason Palmer" , histonet@lists.utsouthwestern.edu 
Sent: Wednesday, 29 July, 2015 2:06:29 AM 
Subject: Re: [Histonet] axolotl lymphatics 

Instead of an antibody, you might consider enzyme activity histochemistry, 
which is much less expensive. 
Demonstration of 5-nucleotidase activity in the presence of levamisole detects 
lymphatic endothelium. Sections can also be stained for alkaline phosphatase 
activity in the endothelium of blood vessels. Here are a few references. 

Kato, S., Yasunaga, A. and Uchida, U. (1991). Enzyme-histochemical method for 
identification of lymphatic capillaries. Lymphology 24 :125-129. 
Ohkuma, M. (1994). Simultaneous double staining for the blood and lymphatic 
capillary. Lymphology 27 , Suppl:106-107. 
Okada, E. (1994). An improved enzyme-histochemical method for identification of 
lymphatic capillaries on paraffin sections. Lymphology 27 , Suppl:732-735. 
Ji, R.C. and Kato, S. (2003). Lymphatic network and lymphangiogenesis in the 
gastric wall. Journal of Histochemistry and Cytochemistry 51 :331-338. 

Needless to say, none of these relate to amphibian tissues! 

John Kiernan 
Anatomy, UWO, London, Canada 
= = = 
On 26/07/15, Jason Palmer via Histonet  
wrote: 



Hi all, 

I need to find an antibody that will label lymphatic endothelial cells in the 
axolotl. Does anybody have any experience or ideas? I have tried a couple of 
our anti-mouse and anti-human Abs for podoplanin and LYVE-1 but no 
cross-reactivity so far. I have no experience with staining of non-mammalian 
tissues - maybe an anti-frog Ab would cross react? Does anyone have experience 
with other amphibians? 

Thanks, 

Jason 



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Re: [Histonet] Missouri Society for Histotechnology Lunch and Learn

[Kelley, Amanda via Histonet]

[X]​Missouri Society for Histotechnology

**Present’s a Fall Lunch n’ Learn**

The Optimization of Fixation & Processing

One of the most fundamentally critical elements of diagnostic histopathology is 
first the ability to suspend all cellular activity in tissue and prevent 
degradation, and secondly to process that specimen in a manner that facilitates 
subsequent steps such as microtomy and staining. The successful result of this 
is a microscopic image of cellular detail that most closely emulates the 
morphologic structure of disease as well as normal tissue.  The concept of 
‘fluid transfer’ will be emphasized as a key factor attributing to the success 
of fixation and processing.  Innovative design in instrumentation will be 
reviewed to better understand how the industry has responded to the demand for 
more efficient methods.  Benefits and selective use of rapid 
fixation/processing will be discussed along with methods of troubleshooting. (2 
CEU’s)

The Circle of Service

The challenges of health care, particularly in the field of Histology, 
oftentimes makes it difficult to deliver quality service. This session focuses 
on the imperative that the Histologist must always remember; that we are an 
integral and critical part of the diagnostic process. This session will detail 
the value and wealth that they to the patient, and therefore that they must 
maintain the standard of 'Quality Care'. (2 CEU’s)

Thank you Sakura Finetek, U.S.A. for your support.

Speaker: H. Skip Brown, M. Div., HT (ASCP)

When:​October 3rd, 2015

Hosted by:

Mercy Hospital

100 Mercy Way

Joplin, MO 64804

Registration: 8:30

Program Time: 9:00 am to 3:00 pm

Lunch Provided

Mercy Conference Room 1. Enter from hospital entrance on east side.

Contact Sharon Walsh for details at 
userwa...@sbcglobal.net

[X]


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Re: [Histonet] Histology Workload

[Michael Ann Jones via Histonet]

We log all ³unbillable² work.
It is by hand and I must tally that by hand each month, but it helps log
what is really being done in our lab.
I tally things like, Histogels, QC control work (slides, blocks,
validations), QIP studies, PT work, send-outs (those do take time), and of
course all of the deepers, stains, IHC, etc.
We charge some clients a ³handling² fee when sending blocks out for
further testing if our pathologists are not involved.
You do need help!

Michael Ann
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
mjo...@metropath.com






On 8/31/15, 3:26 PM, "Cartun, Richard via Histonet"
 wrote:

>Dear Amy:
>
>Some of the companies doing molecular testing will pay you a fee for
>"specimen procurement/handling" since they realize the burden that these
>requests put on us.  I suggest calling the company(s) that you deal with
>and see if you can work something out with them.  And, you're absolutely
>correct; we are being asked to do more work on specimens today, yet
>reimbursements are going down.  Not a good combination.  Based on your
>description of the work that you and your colleagues are doing, you have
>a good case for additional staffing.  It looks like you would benefit
>from a lab assistant that could help with accessioning, billing, and
>send-outs.
>
>Richard
>
>Richard W. Cartun, MS, PhD
>Director, Histology & The Martin M. Berman, MD Immunopathology &
>Morphologic Proteomics Laboratory
>Director, Biospecimen Collection Programs
>Assistant Director, Anatomic Pathology
>Hartford Hospital
>80 Seymour Street
>Hartford, CT  06102
>(860) 972-1596
>(860) 545-2204 Fax
>
>-Original Message-
>From: Amy Self via Histonet [mailto:histonet@lists.utsouthwestern.edu]
>Sent: Monday, August 31, 2015 4:41 PM
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] Histology Workload
>
>Happy Monday to Everyone,
>
>I have been trying[ to figure out how to justify additional help in
>histology - and it's been hard.  My facility staffs according to
>billables.  This by no means feels fair to anyone in this department.
>You could have a specimen that will provide one billable CPT code but can
>produce as many as 20 paraffin blocks.  And now it seems like we are
>getting many request for molecular test that require us to mail the
>patients material out but we have no way of showing that we did this work
>- we call this free work. We get no credit for time spent preparing and
>packaging pathology material to send out to reference labs. Am I missing
>any CPT codes that can be used to show that we in fact did something in
>addition to routine pathology to this case.
>
>We have 1 histotech - 1 histotech/histology supervisor and one histology
>assistant.
>Our block load averages from 125 to 170 daily.
>We also prep non-gyn cytologies.
>Accession all specimens that come in that lab.
>All mail-outs
>The histotech/histology supervisor is responsible for all of the billing
>as well as keeping up with new and old policies/ requirements for CAP/
>auditing billing and the list could go on.
>
>We are drowning in our own workload but don't know how to prove that the
>help is needed.  Any help - advice - suggestions anything will be
>appreciated. How can I prove to upper management that we need more help
>although the billables/productivity numbers say different?
>
>Thanks in advance,
>Amy Self
>Histology Lab Senior Tech
>Lab
>Tidelands Georgetown Memorial Hospital
>606 Black River Road
>Georgetown, SC 29440
>843-520-8711
>as...@tidelandshealth.org
>
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