[Histonet] Staples and Sutures

[Metzger, Kenneth via Histonet]

Hello All,

Recently we have had a marked increase in staples and sutures in our tissue 
that arrives for processing. I have tried to relay to our pathologists and PA's 
the difficulty in cutting sections and the tissue destruction in getting 
staples and sutures out once the tissue is processed to paraffin. I was told by 
some of them that in their experience histology should dealing with this issue. 
Though my own opinion is this is position is not valid, I've been asked to 
collect opinions from other histology departments if there are protocols that 
the grossing individual should be removing the foreign object. Please give me 
any feedback...Thanks

Ken

Kenneth G Metzger HTL(ASCP)
Histology Supervisor
ARUP Labs
Salt Lake City, Utah
Phone: (801)583-2787 ext. 3101
Fax: (801) 584-5244
Email: kenneth.metz...@aruplab.com


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[Histonet] Cassette Printers

[Jason McGough via Histonet]

We have been using a Leica IP-C cassette printer for the past 8 years and have 
experienced some issues this past year with it. Anybody know what the life 
expectancy is of this cassette printer? What other cassette printers work well? 
Thank you in advance for your response.



Jason McGough, HT(ASCP)

Operations Manager

Clinical Laboratory of the Black Hills

605-343-2267

jmcgo...@clinlab.com  

www.clinlab.com  


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[Histonet] RELIA Histology Careers Bulletin 9-29-2015 Are you enjoying the Fall season?

[Pam Barker via Histonet]

Hi Histopeeps!
How are you?  Are you enjoying the sights, sounds and flavors of Fall?
I know I am enjoying college football after all I am a southern gal and you
know how we southerners are about our Football Saturdays in the South.  
 
I am still patiently waiting for "The Real Fall stuff".
You know like crisp cool air, beautiful fall colors,
crunching leaves and steaming bowls of soup.
 
Because it is still 90 degrees here in Orlando
 
 What are you enjoying most of all about this season?
 
I also wanted to tell you about some new job opportunities.  
These are some of my best clients and they are ready to interview and hire
right away.
 
HOT HISTOLOGY OPENINGS:
Histotech - Fayetteville, AR
Histotech - Nashville, TN
Histotech - Reno, NV CLIA qual to gross and NV lic req.
Histotechnician - Atlanta, GA
Histotechnician - Longview, TX (Learn MOHS!!)
Lead Histotech - Atlanta, GA
Histotech - Austin, TX
Histotech - Atlanta, GA
Histotech - East of Chicago - Hammond, IN
 
If you or anyone you know might be interested in any of these opportunities
or would like help with a job search in another area of the USA please
contact me.  I can be reached at rel...@earthlink.net or toll free at
866-607-3542.
 
Remember if I place someone you refer to me you will earn a referral bonus!!
 
Have a great week!!
 
Thanks-Pam
 
Right Place, Right Time, Right Move with RELIA!
 
Thank You!
 Pam M. Barker
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com/PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia

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[Histonet] Dako computer?

[Morken, Timothy via Histonet]

Does anyone have a left-over computer for the Dako Autostainer Plus you want to 
sell? Ours died and the service vendor is having a hard time finding a 
replacement.

Any help is appreciated!


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

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[Histonet] PFIC Case Material

[Cooper, Brian via Histonet]

Good evening Histonet,
Would any of you have access to, and be willing to share either blocks, or 
unstained slides from patients known to have progressive familial intrahepatic 
cholestasis (PFIC2 or PFIC3)?  We're interested in working up a few antibodies, 
yet a sufficient number of positive cases is proving to be elusive.  I'm 
willing to trade for control blocks that may have in our library.  Please 
contact me if you can help.
Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357 Pager: 213-209-0184
bcoo...@chla.usc.edu



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[Histonet] Histotechnician opening in Bourbonnais, IL

[Eric Sulkosky via Histonet]

New Histology lab opening soon in Bourbonnais, Illinois. They are
recruiting for a fulltime histotechnician to accession, gross, process,
embed, cut and stain GI biopsies.

ESSENTIAL JOB FUNCTIONS:

Knowledge:
Demonstrate proficiency with accessioning, grossing, microtomes, tissue
processors, stainers, and equipment used to produce a stained and labeled
slide
Perform microscopic evaluation of own work and uses the information gained
to reduce error and improve performance.
Can recognize positive results.  Can differentiate incorrectly performed
work and correct it.
Reviews all procedure manuals, safety manuals, equipment operator's manuals
and current policy
Demonstrate complete understanding of all test procedures.
Possess leadership and interpersonal skills commensurate with
assigned responsibilities.
Learns specialty areas of test performance.

Physical and Cognitive Requirements:
Ability to master delicate mechanical movements carefully and safely
perform tasks requiring incremental adjustments.
Ability to physically, audibly, and visually (including color
discrimination) perform all functions; with or without reasonable
accommodation.

Education:  A.S. Degree in Histology required, B.S. Degree desired.

Experience:
H.T. and/or H.T.L. (ASCP), or equivalent experience and training required.
Previous experience as a technician or technologist is preferred.
Two years of experience at an acute care hospital of more than 200 beds, or
a reference lab is desired.

Interested candidates should forward resumes to:  toconnor...@gmail.com
Please NO phone calls.
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[Histonet] Processors and Coverslippers

[Fran Pearsall via Histonet]

Hello every one,

We are looking to purchase new equipment for our Histo lab. We are 
interested in the Thermo Gemini AS heated stainer and also the Thermo 
ClearVue Coverslipper. Can any one share their thoughts and opinions on 
these pieces of equipment?  How well do the 2 work together or go with a 
stand alone coverslipper? Thank you for any suggestions.


Fran Pearsall

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Re: [Histonet] Staples and Sutures

[Bob Richmond via Histonet]

Kenneth G Metzger HTL(ASCP), Histology Supervisor, ARUP Labs, Salt Lake
City, Utah asks:

>>Recently we have had a marked increase in staples and sutures in our
tissue that arrives for processing. I have tried to relay to our
pathologists and PA's the difficulty in cutting sections and the tissue
destruction in getting staples and sutures out once the tissue is processed
to paraffin. I was told by some of them that in their experience histology
should dealing with this issue. Though my own opinion is this is position
is not valid, I've been asked to collect opinions from other histology
departments if there are protocols that the grossing individual should be
removing the foreign object. Please give me any feedback...Thanks<<

What la-la land are your pathologists and PAs off in? I've caught plenty of
hell in my life in pathology since 1964 - particularly back before the days
of disposable blades - for not getting staples out of tissue before
submitting it. This is definitely the responsibility of whoever's doing the
grossing.

Bob Richmond
Samurai Pathologist
Maryville TN
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Re: [Histonet] increase in staples and sutures

[Davis, Cassie via Histonet]

RE: increase in staples and sutures 
Ken,
   this really depends on the amount of support you get from your Medical 
Director. Any staple or sutures are removed at grossing by the PA or embedding 
or microtomy by the histology technician still affects the tissue. Either way 
the pathologist hasn't typically seen the tissue or slide yet. This is a fight 
that seems to go on everywhere that does larger specimens. We record it at 
microtomy as a quality issue of grossing if there is a staple or suture in the 
block just for documentation purposes. 
  The only way there can be can justification for the tech removing it is 
the PA time and blade that get ruined cost more than the HT that is embedding 
or doing microtomy. I'm not sure why it is not taken into consideration the 
PA's time and blade is already on the specimen in order to gross it so why 
should both budgets suffer. I wish you well on this fight, please share if you 
make good progress.

Cassie Davis


Message: 2
Date: Tue, 29 Sep 2015 16:10:48 +
From: "Metzger, Kenneth" 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] Staples and Sutures
Message-ID:
<3855827cd3e36249a30d57f6f896f8f10229adc...@exmbx2.aruplab.net>
Content-Type: text/plain; charset="us-ascii"

Hello All,

Recently we have had a marked increase in staples and sutures in our tissue 
that arrives for processing. I have tried to relay to our pathologists and PA's 
the difficulty in cutting sections and the tissue destruction in getting 
staples and sutures out once the tissue is processed to paraffin. I was told by 
some of them that in their experience histology should dealing with this issue. 
Though my own opinion is this is position is not valid, I've been asked to 
collect opinions from other histology departments if there are protocols that 
the grossing individual should be removing the foreign object. Please give me 
any feedback...Thanks

Ken

Kenneth G Metzger HTL(ASCP)
Histology Supervisor
ARUP Labs
Salt Lake City, Utah
Phone: (801)583-2787 ext. 3101
Fax: (801) 584-5244
Email: kenneth.metz...@aruplab.com

 

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error in a separate email.


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Re: [Histonet] Staples and Sutures

[Angela Hall via Histonet]

Ken, 


I'm, currently, checking our SOPs with updates from CAP.  The one that stuck 
out to me under "Quality Management", see below:

**NEW** 07/28/2015
ANP.10038  Anatomic Pathology Checklist 07.28.2015
Tissue Sample Quality
Phase II
There is a procedure that describes the process by which 
histotechnologists provide feedback to submitting pathologists and 
pathology assistants on the quality of the gross tissue sections received 
for tissue processing.
NOTE: Inadequate fixation, overly thick tissue sections, non-   
decalcified bone, the presence of staples, etc., can lead to poor quality   
histologic sections and/or poor quality special stains/special studies.
This requirement applies to both laboratories that gross tissue and 
perform all processing onsite, as well as laboratories that gross tissue 
and send itto another laboratory for processing, embedding, and sectioning 
(regardless of the outside laboratory's accrediting organization).
Records of such feedback and corrective action taken when problems are  
identified may be incorporated into the laboratory's quality management 
program.



Angela D. Hall, BA, HT(ASCP)CM
Histology Department
American Esoteric Laboratories
www.ael-east.com

Tel +423 586 3240 ext 1019 or 1041 Fax +423 714 2001
 

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-Original Message-
From: Metzger, Kenneth [mailto:kenneth.metz...@aruplab.com] 
Sent: Tuesday, September 29, 2015 12:11 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Staples and Sutures

Hello All,

Recently we have had a marked increase in staples and sutures in our tissue 
that arrives for processing. I have tried to relay to our pathologists and PA's 
the difficulty in cutting sections and the tissue destruction in getting 
staples and sutures out once the tissue is processed to paraffin. I was told by 
some of them that in their experience histology should dealing with this issue. 
Though my own opinion is this is position is not valid, I've been asked to 
collect opinions from other histology departments if there are protocols that 
the grossing individual should be removing the foreign object. Please give me 
any feedback...Thanks

Ken

Kenneth G Metzger HTL(ASCP)
Histology Supervisor
ARUP Labs
Salt Lake City, Utah
Phone: (801)583-2787 ext. 3101
Fax: (801) 584-5244
Email: kenneth.metz...@aruplab.com


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recipient.
Unauthorized forwarding, printing, copying, distributing, or use of such 
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Salt Lake City, Utah USA at (+1
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[Histonet] Morgue Refridgerator Temperature

[Fawn Bomar via Histonet]

Does anyone have a procedure for recording their morgue refridgerator 
temperatures that they would be willing to share?  We do not perform autopsies 
at our facility any more but we have the refridgerator that the bodies are 
temporarily stored until they are picked up.  We are monitoring the temperature 
but do not have an actual procedure written.  I was wondering about a few key 
points such as,



1.  What is the temperature range

2.  Do you have a specific time allocated to check the temperature

3.  If a body is placed in the fridge, do you give it a certain amount of time 
to get the temperature back into range

4.  Do you have the other departments fill out a log of some sort to know if 
and when they place bodies in the fridge to account for any temperature changes



Thank you

Fawn Bomar
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the information it contains. 

Thank you
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[Histonet] Problem with ccmount drying out and reexposing tissue sections

[Lewis, Patrick via Histonet]

Hi everyone,

Has anyone experienced a problem with sigmas cc/mount solution.

I liberally cover my section with it to form a protective seal.,  But with my 
current bottle it seems that when it dries it re-exposes the tissue and does 
not form a protective layer.

I end up having to reapply it.   The 2nd apply seems to get me the protective 
layer that I want, but I am concerned that drying/exposure from the first 
application will damage the staining that I have.

I don't want my post staining slides to be exposed to air for a length of time 
between hematoxylin and cover slipping.

This is a fairly recent problem and I am wondering if I just have a bad batch 
of cc/mount.

CAT# C9368-30 ML

Lot# MKBR27838V

I called Sigma and they are sending me a new bottle from a different lot.

But they haven't experienced this problem before with cc/mount.



Patrick Lewis
Research Associate II Bench
Seattle Childrens Research Institute
206-884-1115

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[Histonet] Tissue processor advice for a small start-up

[Caroline Miller via Histonet]

Hi All,

Our company has reached the size where we can justify buying our own
processor! Yippee!!

However that also leads me to the issue of which one to buy :)

We will have very small batches of blocks to process, approx 10 per process
maybe twice per week, but we need the machine to be very responsive to
different processes - we have a microscope that sections and images at the
same time, therefore we do the staining prior to processing, and have to
tweak the protocols to keep enough contrast int the tissue by the end.

In short:
- low volume
- easy to use and change protocols
- good support for servicing and fixing should things go wrong.
- No formalin will touch the machine, and we will want to change out
solvents from the standard alcohols and xylenes (therefore I need a machine
who's warranty would not be affected by this.
- I would love to get this all in one package and buy the service contract
at the same time as the machine, preferably with the same company.
- If it helps with the cost, then I also want to buy a paraffin microtome :)

We are in San Francisco.

Commercial replies are totally acceptable here, but please do not reply all.

Thanks, as ever, histonet!

yours,
mills

-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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[Histonet] Job Opportunity in So California

[dusko trajkovic via Histonet]

Hallo Histonetters,

We have a great job opportunity at Pfizer La Jolla site, inthe Investigative 
Pathology Lab.

Here is a short list of qualifications desired. Emphasis onIF/IHC multiplexing.

 If you can fulfilthese, please contact me via email (dunat...@sbcglobal.net) 
and I will provide you with information on how to apply through the 
properchannels.

This Pfizer site is nestled in one of the most beautifulplaces in Southern 
California, close to Torrey Pines and UCSD. 


Development and conduct of advancedImmunohistochemistry/Immunofluorescence 
procedures on preclinical study samplesin support of the rapidly expanding 
field of immuno-oncology.  Role toserve as a technical expert for the 
discipline and for projects supported. 
• Responsibilities: 
• Technical support of current Immunohistochemistry/Immunofluorescence 
workloadwith primary focus on immuno-oncology projects. 
• Identification of Immunohistochemistry/Immunofluorescence assay/analysisgaps, 
development of work solutions 
• Single plex and multi-plex  Immunohistochemistry/Immunofluorescencemethod 
development/optimization/validation 
• Technical expert for discipline and appropriate project teams/research 
unitworking groups 
• Immunohistochemistry/Immunofluorescence process (simple/complex) mentor 
forjunior colleagues 
• Development Expectation: 
• Image Analysis (chromogenic or fluorescence) – including data 
interpretation,integration and presentation 
• More in depth project team involvement – Drug Safety Team Lead

 Dusko Trajkovic

Scientist-Investigative path Lab

Pfizer Inc, La Jolla

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