[Histonet] IHC on old slides

[Stoll, Kathryn via Histonet]

Hi Everyone,

I have been doing some IHC staining on slides that are 20 years old or more.   
Depending on the antibody the staining is pale in comparison to my control 
slide.  Does anyone have experience with staining older slides?  Do you have 
any tips to share?  The slide storage conditions are not something I can 
control.  I am under the assumption that they were stored at room temperature.
Thank you in advance.

Kathryn Stoll

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] IHC on old slides

[Rene J Buesa via Histonet]

The only thing you can do is to prolong the Ab staining time but, in order to 
avoid excessive background, dilute it. You will have to find an adequate 
balance between a more diluted Ab with a weaker spitope signal and a  prolonged 
incubation time and there is no "magic formula" for obtaining it. You will have 
to make tests.Antigen in stored slides oxidizes producing a weaker signal.René  


 On Friday, November 13, 2015 10:51 AM, "Stoll, Kathryn via Histonet" 
 wrote:
   

 Hi Everyone,

I have been doing some IHC staining on slides that are 20 years old or more.  
Depending on the antibody the staining is pale in comparison to my control 
slide.  Does anyone have experience with staining older slides?  Do you have 
any tips to share?  The slide storage conditions are not something I can 
control.  I am under the assumption that they were stored at room temperature.
Thank you in advance.

Kathryn Stoll

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Histology Related Questions?

[Rene J Buesa via Histonet]

That is OK with me.René 


 On Thursday, November 12, 2015 7:48 PM, Va Paula Sicurello via Histonet 
 wrote:
   

 Hello Netters,
I am taking a research techniques class for my MBA this term and need to have a 
project that I can ask quantifiable questions.  I was thinking about 
productivity and benchmarks in the Histology and IHC labs.
If I send an email with my questions, will you be kind and answer them in a 
reply email?
I hope to keep it between 15-20 questions.
I even promise to share my results once I compile all the information.
Suggested questions are also gratefully accepted.
Toodles! Sincerely,
Paula
Paula SicurelloHistotechnology SpecialistUC San Diego Health
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] #ExtMail# Slide and cassette printers

[Ellenburg, Deborah via Histonet]

We have the ShurMark slide and cassette engraver that interfaces with CoPath.  

Sent from my iPhone

> On Nov 12, 2015, at 10:50 AM, Mike Pence via Histonet 
>  wrote:
> 
> Okay, I am reaching out to the people that I know that has an answer to 
> everything. What system are you using to print slides and cassettes from in 
> your lab? Is it intergraded with your LIS and if so what LIS are you using? I 
> am not looking for a system that prints to labels, I what it to print 
> directly to the slide or cassette.
> 
> Michael S. Pence | Supervisor of Laboratory Services
> Great River Health Systems
> 1221 S. Gear Ave. | West Burlington, IA 52655
> Office 319-768-4546 | Main 319-768-4525 | Fax 319-768-4557
> mpe...@grhs.net | 
> www.greatrivermedical.org.
> www.Facebook.com/GreatRiverHealthSystems
>  | www.Twitter/GreatRiverMed
> 
> 
> Information in this communication, including attachments, is confidential and 
> intended only for the addressee(s). This communication may contain 
> privileged, confidential, proprietary or trade secret information entitled to 
> protection or exemption from disclosure under law. If you are not an intended 
> recipient, please know that any use, distribution or copying of this 
> communication, or any action taken based on the information in this 
> communication, is unauthorized and may be unlawful. If you received this 
> communication in error, please notify the sender and delete this 
> communication from your device.
> 
> 
> 
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this communication is intended to be confidential to the 
Individual(s) and/or Entity to whom it is addressed.
It may contain information of a Privileged and/or Confidential nature, which is 
subject to Federal and/or State privacy regulations.
In the event that you are not the intended recipient or the agent of the 
intended recipient, do not copy or use the information
contained within this communication, or allow it to be read, copied or utilized 
in any manner, by any other person(s). Should
this communication be received in error, please notify the sender immediately 
either by response e-mail or by phone,
and permanently delete the original e-mail, attachment(s), and any copies.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Mohs sections

[Angela Yepes via Histonet]

> From: Angela Yepes 
> Date: November 12, 2015 at 6:05:57 PM EST
> To: histonet@lists.utsouthwestern.edu
> Subject: Mohs sections
> 
> 
>> From: Angela Yepes 
>> Date: November 12, 2015 at 4:15:50 PM EST
>> To: histonet-requ...@lists.utsouthwestern.edu
>> Subject: Mohs sections
>> Hello everyone:
>> While cutting Sections for Mohs surgery I noticed the tissue detaching from 
>> the O.C.T. Embedding medium  and subsequent rolling of the tissue.
>> To correct the problem the chuck was rotated to allow cutting from the 
>> opposite side (epidermis side). This maneuver did not correct the problem. I 
>> tried to unroll the edges with stiff brush which seemed to correct the 
>> problem, but it did not. I only  found out the sections were still rolling 
>> up after the staining.
>> The cryostat temperature was -23C and the sections thickness was -14 microns 
>> Please see pictures.
>> Please help 
>> 
>> 
>> 
>> 
>> Sent from my iPhone
>> 
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] IHC on old slides

[Hobbs, Carl via Histonet]

Amplification is the only way.

Possibly, tyramide amplification will help.

It will cost you to buy a kit...sure, make your own butyou have to weigh up 
pros/cons


Sureyou will have to play with variables.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet