[Histonet] microtome issues: thick and no sections

[Tyrone Genade via Histonet]

Hello,

I am having a problem with sectioning.

I'm cutting 5 um sections but with the turn of the microtome I will miss
the block, or take a small piece of the block/tissue and then the next
section ends up thick. This missing can take several turns of the wheel so
I can get really big sections. I have checked the angle of the knife to the
block and the tension along the blade but these are fine. I worry that the
issue might be temperature. The microtome is on a bench against an outside
wall. The temperature is about 15 oC. Could this be the issue?

I am not cooling the block on ice. Previous experience with block-cooling
was that I would get thick and thin sections that way... this is a very
different problem.

Thanks

-- 
Tyrone Genade
Orange City, Iowa
tel: (+1) 712 230 4101
http://tgenade.freeshell.org

Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord.
To find out how to receive this FREE gift visit http://www.alpha.org.
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[Histonet] Thick and thin sections

[Carlos Defeo via Histonet]

Tyrone: definitely no temperature issue in this case. Look if your using 
cassete that is correctly clamped, and then if the answer is no,

check the other parts of the blade holder unit.
If still no part seems to be loosen, you have to service the micrometric 
advance, what seems to be the most reasonable cause of the problem.

Good luck and my kind regards,
Carlos.-
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[Histonet] Al Floyd, a great loss to NSH

[Morken, Timothy via Histonet]

For those that knew him or took courses from him, Al was a consummate 
professional. I was fortunate enough to work with him on some projects and 
found his knowledge and insight invaluable. He was also a good friend. He will 
be missed, especially as a fixture at NSH meetings.


Tim Morken
Pathology Site Manager, Parnassus
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center


[http://ak-cache.legacy.net/legacy/images/cobrands/southbendtribune/photos/0020265377-01-1_20151101.jpg?v=0x330702d6]
Alton David Floyd July 17, 1941 - Oct. 29, 2015 EDWARDSBURG - Alton D. Floyd, a 
true renaissance man, passed away Thursday, October 29, 2015. He was born to 
the late Frank and Queen Tina (Melton) in Henderson, Kentucky, on July 17, 
1941. He was a Kentucky farm boy who excelled in 4-H and played trumpet in high 
school and the University of Kentucky marching band. Upon graduation from the 
University of Kentucky, Alton earned a Ph.D. from the University of Louisville, 
followed by six years on the faculty at the University of 
Michigan
 Medical School and thirteen years at Indiana University Medical School. He 
married the love of his life, Barbara Wilson, with whom he celebrated 53 years 
of marriage this past July. Surviving are his wife Barbara of Edwardsburg, 
Michigan, and their two daughters, Fara McCune and her husband, Michael, and 
their children, Liam, Finnian and Fiona; and Heather Floyd and 
 her husband, Dan Willems; as well as his sister, Brenda Herron. The last 30 
years of Alton's career were spent as a consultant in the pharmaceutical and 
medical diagnostic industries. He derived great satisfaction from teaching many 
years at the annual National Society for Histotechnology. He also served on the 
Board of Directors for several organizations. Alton also loved music, 
especially Jazz. He valued his membership in the Michiana Amateur Radio Club 
where he was known by the call sign W8ADF, and attending their Tuesday 
afternoon luncheons. Retirement gave him the opportunity to enjoy many artistic 
pursuits: designing and creating jewelry, participating in classes at the Krasl 
Art Museum, and woodworking - from intricate carvings to making furniture, 
painting and stained glass. He especially enjoyed lake activities each summer 
with his grandchildren. Alton passed the same way he lived, with extreme grace 
and dignity. There will be no services at this time, but a celebration of 
 his life is planned for next summer. Elkhart Cremation Service is assisting 
with the arrangements.
- See more at: 
http://m.legacy.com/obituaries/southbendtribune/obituary.aspx?n=alton-floyd=176297459=0=false#sthash.IbF8q928.dpuf
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