[Histonet] Validation Process or Procedure for the Shandon Cytospin 4 and the Sorvall ST 8 Centrifuge
Does anyone have a validation protocol to share? Mr B ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Histonet Digest, Vol 146, Issue 18 ( Microtome Thick and Thin issues)
Tyrone I am in agreement with Carlos except low operating temperature can effect the lubrication of the slide mechanism by gelling up and causing the slide mechanism to skip and jump. Lubrication of the internal mechanics of the Microtome are very necessary on a regular basis. If volumes use is high then lubricants dry out causing skipping and jumping of the slide. Either way my recommendation is to have it Serviced right away and do not use any more until you have done so. Brent Adams – BS, LPN, HT www.acadianagastro.com Acadiana Gastroenterology Associates, LLC 439 Heymann Blvd Lafayette, LA 70503 tel: (337) 269-1126 fax: (337) 269-1476 PRIVILEGED AND CONFIDENTIAL: This document and the information contained herein are confidential and protected from disclosure pursuant to Federal Law. This message is for the designated recipient only and may contain confidential, privileged, proprietary, or otherwise private information. If you have received this email in error, please notify the sender immediately and delete the original with any attachments. Any other use of the email is strictly prohibited. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] microtome issues: thick and no sections (Tyrone Genade)
Hello, Thanks to all who responded to my question. The solution, it seems was very simple. Several people reported similar experiences and issues with the specimen clamp not fitting tightly and that I should clean the clamp or block. My blocks are clean and I thought the clamp was too... but when I ran a set of forceps along the inner, upper edge of the clamp a tiny blob of wax was found. A test block cut normally. I'm amazed a tiny piece of wax could cause so much trouble! Thanks to everyone who offered help. Bye ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Pinkerton's adaptation of machiavello's stain for chlamydophila on tissue sections
Hi there, I was wondering if any of you had used the Pinkerton's adaptation of machiavello's stain for staining chlamydophila on parafin tissue sections and whether it works or not. I would also be very grateful if anybody can advise in any other technique working on tissue sections. We are working with an ovine abortion here. Best regards, Julio benavides ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
