[Histonet] Can't get the calculation right today
Please, please and please help me. I am fighting a sinus infection and I can not think to save my life. I have to make a buffer solution: 10mM Tris-HCL, 5mM CaCl2, 50% glycerol (v/v), pH7 I have tris base and calcium chloride powder. Can someone please help me by writing it out for me? I am so stuck. Thank you, Eva (the one whose head is about to explode) ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Clinical Lab vs. Antomical Pathology
In my past career in a medium sized community hospital, both aspects of the lab were melded. But your issue with the frozen sections is a different one. Your director needs to meet with the O.R. or the infection control staff to find a way to allow you into the suite without violating any established protocols. If you are actually entering the O.R., you really should check to see if there is a policy that requires changing scrubs between O.R. area and the lab. Lester Raff, MD Uropartners Laboratory Unrelated (and politally inclined) Blog post: http://www.chicagonow.com/downsize-maybe/2016/03/donald-trump-takes-on-artificial-intelligence/ -Original Message- From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Monday, March 14, 2016 2:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Clinical Lab vs. Antomical Pathology Good Morning, I am curious to know how many Pathology Departments get lumped into Clinical Lab? Do you wear the same working attire? Our Pathology Department is really small. We are required to go over the red lines in surgery to get to our Frozen Section Room numerous times a day. They want to put us in the same Scrubs as the Clinical Lab which would not allow us to go over the red line. The color of scrubs designates where you are from in the hospital. I for one do not know how to draw blood or even know what color top tube to use the CLS and phlebotomist do. I only know only about Bone Marrows. I am getting frustrated because to me Anatomical Pathology and Clinical Lab is two different species but they keep trying to treat us the same. The only thing we share is the Lab Director and the Pathologists with Clinical Lab. Feeling like I am not being heard. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Clinical Lab vs. Antomical Pathology
Good Morning, I am curious to know how many Pathology Departments get lumped into Clinical Lab? Do you wear the same working attire? Our Pathology Department is really small. We are required to go over the red lines in surgery to get to our Frozen Section Room numerous times a day. They want to put us in the same Scrubs as the Clinical Lab which would not allow us to go over the red line. The color of scrubs designates where you are from in the hospital. I for one do not know how to draw blood or even know what color top tube to use the CLS and phlebotomist do. I only know only about Bone Marrows. I am getting frustrated because to me Anatomical Pathology and Clinical Lab is two different species but they keep trying to treat us the same. The only thing we share is the Lab Director and the Pathologists with Clinical Lab. Feeling like I am not being heard. Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Processing human brain xenografts
Hello Histonetters, I was wondering if anyone could offer advice for the processing of our human brain xenografts harvested from mice. The tumor specimens are approximately 0.5 x 0.5 x 0.5cm. We have been putting them on our animal program ( 30 min alcohols) but once embedded, the tissue looks brittle and some shrinkage occurs. The morphology looks okay. S hould we put them on our routine program ( 1 hour ethanols and xylenes) or is brain tissue too delicate for that run? Would appreciate any advice. Thank you, Valerie --- Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and/or privileged information. If you are not the intended recipient(s), you are hereby notified that any dissemination, unauthorized review, use, disclosure or distribution of this email and any materials contained in any attachments is prohibited. If you receive this message in error, or are not the intended recipient(s), please immediately notify the sender by email and destroy all copies of the original message, including attachments. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Cryostat with UV disinfection
Our Mohs Lab is CAP accredited. Every seven days of use, cryostats are shut down. Once machine has been cleaned and dried we use Cavi-wipes(super Sani-Cloth from PDI Inc.) We then document disinfection date on QC Log. We have three cryostats, we rotate a cryostat a week for disinfecting purposes. We added this to our Equipment: Cryostats/Microtomes policy. Hope this helps. Karen Pfaff, HT (ASCP), BA Lead Histotechnician Skin Cancer Center Froedtert Health Phone: 414-805-6010 Fax: 414-805-5325 -Original Message- From: Terri Braud [mailto:tbr...@holyredeemer.com] Sent: Friday, March 11, 2016 12:52 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Cryostat with UV disinfection For those of you that are CAP accredited, and use a cryostat with UV disinfection, how do you get around the CAP requirement listed below? We were told the UV disinfection did not replace wiping down with a tuberculocidal disinfectant at room temp. Do you have an approved tuberculocidal disinfectant that will work at cryostat temps? Curious minds would like to know! ANP.23410 Cryostat DecontaminationPhase II There is a written procedure for the decontamination of the cryostat at defined intervals, and under defined circumstances, and decontamination records are evident. NOTE: The cryostat must be defrosted and decontaminated by wiping all exposed surfaces with tuberculocidal disinfectant. The cryostat should be at room temperature during decontamination unless otherwise specified by the manufacturer. This should be done at an interval appropriate for the institution; this must be weekly for instruments used daily. Trimmings and sections of tissue that accumulate inside the cryostat must be removed during decontamination. Although not a requirement, steel mesh gloves should be worn when changing knife blades. Terri L. Braud, HT(ASCP) Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
