Re: [Histonet] Histonet Digest, Vol 149, Issue 5

[Douglas Gregg via Histonet]

Expired antibodies. Hmm.

I got this Himalayan sea salt that says it was created 250 million years
ago. Label says the expiration date is 2017. Guess they dug it up just in
time.

Doug Gregg :-)

On Tue, Apr 5, 2016 at 1:00 PM, 
wrote:

> Send Histonet mailing list submissions to
> histonet@lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-requ...@lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-ow...@lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
>1. IHC Staining (Pardue, Judith)
>2. expired antibodies (Eck, Allison)
>
>
> --
>
> Message: 1
> Date: Tue, 5 Apr 2016 09:42:53 +
> From: "Pardue, Judith" 
> To: "histonet@lists.utsouthwestern.edu"
> 
> Subject: [Histonet] IHC Staining
> Message-ID:
> <
> f8593d93099a864a9730359fa6992a879279f...@chiex016.chi.catholichealth.net>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Is anyone having a problem with red chromogen verses brown staining on
> HMB-45. We are having inconsistent staining with the red on HMB-45.
>
>
> Judith Pardue
> Histology Supervisor
> CHI Memorial Health Care
> Chattanooga, Tn.
> judith_par...@memorial.org
>
> This email and attachments contain information that may be confidential or
> privileged. If you are not the intended recipient, notify the sender at
> once and delete this message completely from your information system.
> Further use, disclosure, or copying of information contained in this email
> is not authorized, and any such action should not be construed as a waiver
> of privilege or other confidentiality protections.
>
>
> --
>
> Message: 2
> Date: Tue, 5 Apr 2016 15:01:46 +
> From: "Eck, Allison" 
> To: "'histonet@lists.utsouthwestern.edu'"
> 
> Subject: [Histonet] expired antibodies
> Message-ID:
> <4ed8c96a8f20fc4f883a92e2a0a0d64a97235...@dh-mail01.dhorg.org>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anyone know of anyone who will accept expired antibodies for research
> or educational purposes?
> I appreciate any feedback
> Thanks
>
> Allison Eck, HTL(ASCP)cm, AHI(AMT)
> Lead Tech Histology
> Doylestown Hospital
> 595 W State St
> Doylestown, PA 18901
> 215-345-2264
> a...@dh.org
>
>
>
>
> --
>
> Subject: Digest Footer
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> --
>
> End of Histonet Digest, Vol 149, Issue 5
> 
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Looking for an interesting conversation regarding RNA and Formalin fixation

[Caroline Miller via Histonet]

Hi there Histonet,

I am looking to have a conversation regarding regular histology processing,
mainly formalin fixation, on the RNA species of cell.

I know there is a lot of problem in this space and would like to talk to
someone who is embedded in this world and could offer advice / services to
accurately analyse RNA in formalin fixed, paraffin embedded (and even maybe
resin) tissues.

Asking a lot - yes, I know! However there are a lot of bright folks out
there that might just have an answer for me!

Please PM me!

yours,
mills

-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Validating IHC on decalcified specimens

[Elizabeth Chlipala via Histonet]

Teri

We have a spot on all of our IHC SOP's that will state if the antibody has been 
tested on decalcified samples.   We do not routinely add decalcified tissue to 
our routine IHC protocol development runs, so for example if the target is a 
soft tissue target we will develop on a variety of soft tissues that express 
different  staining intensities.  If the target is expressed in bone and 
decalcified bone is the target tissue we will work with both decalcified and 
soft tissue during the development process.If we are taking a previously 
soft tissue antibody and testing it in bone or a sample that has been 
decalcified then that information is added to our internal tracking documents 
and then the SOP as a revision, we follow the same protocol if we are testing 
on another species that we have not previously stained for.  

I can give you an example of the last scenario.  We are frequently requested to 
run GFAP IHC sometimes the samples are routinely fixed on other occasions the 
skull has been left intact and the sample decaled, we initially developed and 
verified on soft tissue but later added to the verification that the antibody 
was tested on decaled samples and we would list the decalcification method/s 
tested in the SOP.  I'll send off examples of both our GLP and non-GLP IHC 
protocols in a different e-mail.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-Original Message-
From: Teri Johnson via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, April 05, 2016 12:50 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Validating IHC on decalcified specimens

Hi Histonetters,

Can someone tell me if they do anything specific to validate their IHC markers 
on decalcified specimens? If no, do you test them anyway?

Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA  92008
USA

Phone +1 760 516 5954
tejohn...@genoptix.com
www.genoptix.com




CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and contains information that is 
confidential and proprietary to Genoptix Medical Laboratory or its 
subsidiaries. Any unauthorized review, use, disclosure or distribution is 
prohibited. If you are not the intended recipient, immediately contact the 
sender by e-mail and destroy all copies of the original message.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Validating IHC on decalcified specimens

[Cartun, Richard via Histonet]

I have a validation file for ER, PR, and HER2 IHC testing on decalcified bone 
specimens with metastatic breast cancer.  I compared the results with other 
specimens from the same patient (usually the primary breast cancer) that were 
not decalcified.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & The Martin M. Berman, MD Immunopathology & Morphologic 
Proteomics Laboratory
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-Original Message-
From: Teri Johnson via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, April 05, 2016 2:50 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Validating IHC on decalcified specimens

Hi Histonetters,

Can someone tell me if they do anything specific to validate their IHC markers 
on decalcified specimens? If no, do you test them anyway?

Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA  92008
USA

Phone +1 760 516 5954
tejohn...@genoptix.com
www.genoptix.com




CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and contains information that is 
confidential and proprietary to Genoptix Medical Laboratory or its 
subsidiaries. Any unauthorized review, use, disclosure or distribution is 
prohibited. If you are not the intended recipient, immediately contact the 
sender by e-mail and destroy all copies of the original message.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This e-mail message, including any attachments, is for the sole use of the 
intended recipient(s) and may contain confidential and privileged information. 
Any unauthorized review, use, disclosure, or distribution is prohibited. If you 
are not the intended recipient, or an employee or agent responsible for 
delivering the message to the intended recipient, please contact the sender by 
reply e-mail and destroy all copies of the original message, including any 
attachments.

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Validating IHC on decalcified specimens

[Teri Johnson via Histonet]

Hi Histonetters,

Can someone tell me if they do anything specific to validate their IHC markers 
on decalcified specimens? If no, do you test them anyway?

Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA  92008
USA

Phone +1 760 516 5954
tejohn...@genoptix.com
www.genoptix.com




CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for 
the sole use of the intended recipient(s) and contains information that is 
confidential and proprietary to Genoptix Medical Laboratory or its 
subsidiaries. Any unauthorized review, use, disclosure or distribution is 
prohibited. If you are not the intended recipient, immediately contact the 
sender by e-mail and destroy all copies of the original message.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Used equipment

[John Ronan via Histonet]

Hi Histonetters,

I worked in an in-office histology lab for 4 years and the lab recently
closed. The equipment is in good working condition and is being offered for
sale at very good prices.

On behalf of Gary Casino, Practice Manager at Somerset Urological
Associates in Somerville NJ, I want to let you know that the following
equipment is for sale. Please let me know if you you are interested or need
pictures or more info.

John Ronan

johnro...@custompathlabsolutions.com

GROSS LAB JUNIOR$1800

SHANDON WAX DISPENSER$300

SHANDON
CONVECTION OVEN$300

22 GAL EAGLE FLAMMABLE STORAGE CABINET$575 in excellent condition

17 GAL
JUSTRITE  FLAMMABLE STORAGE CABINET$90.00

SAKURA
DRS 2000 AUTO SLIDE stainer$5000

Tissuewave 2 microwave processor$5,000
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Mycoplasma

[Bernice Frederick via Histonet]

Hello all,
Will PAS stain mycoplasma?
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Mycoplasma

[Normington Lacy via Histonet]

I do not believe it will stain with PAS.  The bacteria lack a cell wall.

-Original Message-
From: Bernice Frederick via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, April 05, 2016 1:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mycoplasma

Hello all,
Will PAS stain mycoplasma?
Bernice

Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-freder...@northwestern.edu

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IHC Staining (Pardue, Judith)

[Greg Dobbin via Histonet]

Judith,
The red chromagen is suseptible to "fading" if stained slides are left in
either alcohol or xylene for too long. Check to make sure that everyone
involved is following the same procedure for the dehydraytion, clearing and
mounting of the stained slides.
Greg

-- 
*Greg Dobbin*
1205 Pleasant Grove Rd
RR#2 York,
PE  C0A 1P0

   med*lab*professionals.ca

*Everything in moderation...even moderation itself**!*
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Used VIP 5 parts

[Kimberly Marshall via Histonet]

Hello fellow Histo folks.  I am looking for some advise on purchasing used 
parts for a VIP 5.  Looking for the bottles.  Thanks in advance


Kimberly Marshall H.T.(ASCP)

Histology/Lab Supervisor

Toll Free 1-800-426-2099

Fax 801-584-5104

PO Box 17580

Salt Lake City, Utah 84107

www.animalreferencepathology.com



Advancing the art and science of veterinary medicine



[cid:image001.jpg@01CF8F87.A0BD4830]

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] expired antibodies

[Eck, Allison via Histonet]

Does anyone know of anyone who will accept expired antibodies for research or 
educational purposes?
I appreciate any feedback
Thanks

Allison Eck, HTL(ASCP)cm, AHI(AMT)
Lead Tech Histology
Doylestown Hospital
595 W State St
Doylestown, PA 18901
215-345-2264
a...@dh.org


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] IHC Staining

[Pardue, Judith via Histonet]

Is anyone having a problem with red chromogen verses brown staining on HMB-45. 
We are having inconsistent staining with the red on HMB-45.


Judith Pardue
Histology Supervisor
CHI Memorial Health Care
Chattanooga, Tn.
judith_par...@memorial.org

This email and attachments contain information that may be confidential or 
privileged. If you are not the intended recipient, notify the sender at once 
and delete this message completely from your information system. Further use, 
disclosure, or copying of information contained in this email is not 
authorized, and any such action should not be construed as a waiver of 
privilege or other confidentiality protections.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet