[Histonet] Several New Histology Career Opportunities!!

[Sarah Kelly via Histonet]

Hello Histonet,



Our exclusively retained client has opened several new opportunities across
the U.S., and I wanted to reach out regarding a few specifics and see if
anyone would be interested in learning more.



Our client is a global leader in cancer diagnostics and we are looking for
individuals with a strong histology background, IHC, and troubleshooting
experience.  The company is part of a $20 billion organization and growing.



These positions are full-time, direct-hire roles with competitive salaries
and full benefits.



If you or anyone you know may be interested in learning more about these
opportunities, please contact me directly at *s...@personifysearch.com
*.





Thank you!



Sarah



Sarah Kelly

Personify, Talent Management Executive

*s...@personifysearch.com *

(800) 875-6188, ext.153
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Cytology specimens

[Martin, Gary via Histonet]

I would like to poll the Histo group concerning cytology specimens. The
discussion has come up about when to discard a fluid after processing,
and fluids that have no orders. Also, because we are a contracted
service for the hospital, and are not connected to their LIS. Cytology's
that are ordered do produce a requisition that sometimes does not follow
the specimen, which leads to missed processing. How are others handling
these two situations. 

Thanks 

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Tissue controls

[Rachel M Gonzalez via Histonet]

Hi Everyone,


I am trying to help out a coworker with a survey on types of tissue and
controls needed for both clinical and research labs. She has a short
deadline by next Monday.

She if in charge of the OriGene Technologies tissue division.  If you could
take a few minutes to fill in the survey for a really nice coworker (Julie)
that would be great.

https://www.surveymonkey.com/r/QMGWKHZ


Thanks for any help.

Rachel
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] (no subject)

[Scott, Catherine L via Histonet]

UT Path located in the medical center (Houston) is currently looking for a 
Medical Technologist that is able to work on an as needed basis, hours are 
negotiable. Duties include, but not limited to performing molecular testing 
using the Hologic Panther system, this could lead to a full time position for 
the right candidate.

In addition, to the MT position, we also have available a full time HTL 
position performing immunohistochemical staining utilizing the Dako system.

We offer completive salaries and great benefits.  Please contact me at 
713-500-5401 or Catherine.L.Scott @uth.tmc.edu.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] PAX 2

[Jeffrey Robinson via Histonet]

Good Morning-  can anyone give me a recommendation for a predilute PAX2 
antibody?  The one I was using has been discontinued and the replacement 
product does not seem to perform very well.  I will be using it on the Bond 
III.  Thanks in advance!
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.


This email and attachments may contain PHI that is privileged and confidential 
and is not intended for any unauthorized person. If you, the reader, are not 
the intended recipient you are hereby notified that any dissemination, 
distribution or copying of this communication is strictly prohibited. Do not 
read the email but instead reply to the sender and destroy the message and any 
attachments. Thank you.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Spill kits

[Lester Raff MD via Histonet]

We keep our spill kits in unobstructed cabinets with bright yellow signage on 
the wall above. We have no problem getting to them as needed, and have never 
had an issue in many, many CAP inspections.
--
--

Latest post: 
http://www.chicagonow.com/downsize-maybe/2016/06/fathers-day-and-lee-the-dad-i-hardly-knew/

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203


-Original Message-
From: Anne Murvosh via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 14, 2016 9:00 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Spill kits

Do spill kits need to be out where anyone can see them, or can I put it in a 
cupboard with a sign on the door?  I need to move mine and there's very little 
room.  Thanks Anne ___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Spill kits

[Rene J Buesa via Histonet]

What you need to do is to communicate to everybody where the kits are, and 
place them where it is more convenient for you. Once everybody knows the 
location, a good sign is always a plus.René 

On Tuesday, June 14, 2016 10:15 AM, Anne Murvosh via Histonet 
 wrote:
 

 Do spill kits need to be out where anyone can see them, or can I put it in a 
cupboard with a sign on the door?  I need to move mine and there's very little 
room.  Thanks Anne
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Over-processing of brain tissue

[Rene J Buesa via Histonet]

It seems to me you are processing too much unless the slices are 3mm thick or 
more.I suggest you to cut the dehydration to 45 minutes (the sequence seems 
OK)Reduce the pure 2-propanol to just 2 changes (30 min is OK)Add 1 change of a 
mixture 1:1 of 2-propanol and xylene + 2 xylene stepsthen to paraffinUse vacuum 
and mixing/agitation in all steps (it will help)If you want really to simplify, 
use 2-propanol in all steps instead of ethanol and eliminate the xylene. You 
can go from pure 2-propanol → 1:1 mixture of 2-propanol with paraffin → 3 
paraffin changes.You will eliminate xylene and obtain very good results.Contact 
me if you need some publications on this procedure.René 

On Tuesday, June 14, 2016 9:53 AM, "Wheelock, Timothy R. via Histonet" 
 wrote:
 

 Good morning everyone:

I seem to be having problems with over-processing of brain tissue.
I use a VIP6 processor.
I start off with 1 hour each of 30%, 50%, 80%, and 95% isopropanol.
Then half-hour each of three changes of absolute isopropanol.
Then half hour each of three changes of xylene.
Then half-hour each of 4 changes of Paraplast.

I use a slow mixing cycle and no vacuum-pressure for all reagents except the 
paraffin.
For the paraffins, I use vacuum pressure, but no mixing cycle.

I rotate the paraffins during each run of tissue.
I replace the dilutions of isopropanol after I have processed 500 blocks.
I rotate the absolute isopropanols and xylenes after 500 blocks as well.

When I embed the brain tissue, it sometimes seems a little stiff, or even a bit 
brittle, to one extent or another.
When I trim the blocks, they seem somewhat dry. Once in a while there is even a 
saw-dust effect.
Before I section the blocks, I have to keep them on ice for at least 3 hours 
before they are moist enough to cut.
Even then, the first case (out of 5 cases) shows chatter in the cerebral cortex.
When I examine the stained sections microscopically, even the best sections 
look a little "rough" or dry.
Taking microscopic images can be difficult at 40x ( or even 20x) because all 
this roughness shows.
The brain tissue looks "granular" rather than smooth, especially with a LHE 
stain.

I have continued to reduce the times to their present values, but it still does 
not seem enough.
I absolutely love the VIP6, but it is a much more powerful machine than the 
Shandon Hypercenter XP that I use to have.
Perhaps I have still not fully compensated for this power.
It has 4 paraffin reservoirs rather than 2 on the old Hypercenter, and the 
reagent reservoirs hold twice as much volume.

Any ideas on how to resolve this problem?
Should I reduce the times further?
Should I alter the use of the mixing and/or vacuum-pressure?

Thanks for any help that you can give me.

Tim


Tim Wheelock
Assistant Director, Neuropathology
Instructor of Neuroanatomy
Tour Coordinator
Harvard Brain Tissue Resource Center
Room 203, Mailman Research Center
McLean Hospital, Belmont, MA 02478
Phone: 617-855-3592
Cell:    857-234-9311
Fax:    617-855-3199



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


  
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Help with liver/heart tissues

[Allyse Mazzarelli via Histonet]

Good afternoon,

I am having a few issues getting nice morphology from both liver and heart
tissues. I currently work only with CNS tissues (brain, spinal cord, DRG,
etc.) but recently, our research team have become interested in
immunostaining some peripheral tissues, including the heart and liver.

All tissue [mouse] is perfusion fixed with 4% PFA and then post-fixed in
fresh 4% PFA for at least 24 - 48 hours. After fixation, the tissue is
cryoprotected in 30% sucrose solution for 48 hours. Once cryoprotected, I
then section the tissue at various thicknesses depending on the assay I'm
running.

Previously I sectioned some heart and liver samples (sectioned both at 20um
and at 8um) and ran a few H Unfortunately, the tissue was so damaged
and under-fixed that we scrapped the blocks.

This time around, the mouse liver tissue was carefully dissected prior to
post-fixation into four quadrants to allow for better PFA permealization.
Additionally, we post fixed in 4% PFA for 3 days instead of 2, and the
tissue was cryoprotected for 2 days.

To my surprise, when I sectioned this tissue on the cryostat, I still
noticed severe artifacts. It is very difficult to see nice morphology, and
there appears that there was an issue with fixation (in the liver the
nucleus is flattened and the sinusoids are not clear, etc. the nuclei in
the heart are also more flat and the muscle fibers have separated from one
another). The staining was not as bad as the first tissue I had sectioned,
but I was still unable to get a nice H stain depicting clear
nuclear/cytoplasm. These artifacts appeared at both 8um and 20um.

Does anyone happen to have nice fixation/H staining protocols for both
liver and heart? I'd be happy to give an in-depth description of the
protocol(s) I'm currently using. Additionally, is there anything that
appears I'm doing wrong in terms of perfusion/fixation?

Thanks so much!

Allyse
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Spill kits

[Anne Murvosh via Histonet]

Do spill kits need to be out where anyone can see them, or can I put it in a 
cupboard with a sign on the door?  I need to move mine and there's very little 
room.  Thanks Anne
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Over-processing of brain tissue

[Wheelock, Timothy R. via Histonet]

Good morning everyone:

I seem to be having problems with over-processing of brain tissue.
I use a VIP6 processor.
I start off with 1 hour each of 30%, 50%, 80%, and 95% isopropanol.
Then half-hour each of three changes of absolute isopropanol.
Then half hour each of three changes of xylene.
Then half-hour each of 4 changes of Paraplast.

I use a slow mixing cycle and no vacuum-pressure for all reagents except the 
paraffin.
For the paraffins, I use vacuum pressure, but no mixing cycle.

I rotate the paraffins during each run of tissue.
I replace the dilutions of isopropanol after I have processed 500 blocks.
I rotate the absolute isopropanols and xylenes after 500 blocks as well.

When I embed the brain tissue, it sometimes seems a little stiff, or even a bit 
brittle, to one extent or another.
When I trim the blocks, they seem somewhat dry. Once in a while there is even a 
saw-dust effect.
Before I section the blocks, I have to keep them on ice for at least 3 hours 
before they are moist enough to cut.
Even then, the first case (out of 5 cases) shows chatter in the cerebral cortex.
When I examine the stained sections microscopically, even the best sections 
look a little "rough" or dry.
Taking microscopic images can be difficult at 40x ( or even 20x) because all 
this roughness shows.
The brain tissue looks "granular" rather than smooth, especially with a LHE 
stain.

I have continued to reduce the times to their present values, but it still does 
not seem enough.
I absolutely love the VIP6, but it is a much more powerful machine than the 
Shandon Hypercenter XP that I use to have.
Perhaps I have still not fully compensated for this power.
It has 4 paraffin reservoirs rather than 2 on the old Hypercenter, and the 
reagent reservoirs hold twice as much volume.

Any ideas on how to resolve this problem?
Should I reduce the times further?
Should I alter the use of the mixing and/or vacuum-pressure?

Thanks for any help that you can give me.

Tim


Tim Wheelock
Assistant Director, Neuropathology
Instructor of Neuroanatomy
Tour Coordinator
Harvard Brain Tissue Resource Center
Room 203, Mailman Research Center
McLean Hospital, Belmont, MA 02478
Phone: 617-855-3592
Cell: 857-234-9311
Fax: 617-855-3199



The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in error
but does not contain patient information, please contact the sender and properly
dispose of the e-mail.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet