[Histonet] Automatic ordering

[Rachel Pinch via Histonet]

Does anyone have a pathologist that requires a special stain or HP IHC's ran
on ALL upper GI cases? Is it overutilization or do you feel it is for
verification purposes? 

 

Rachel Pinch, HT(ASCP)

Laboratory Operations Manager

GALA Histology Lab

3030 S. Gessner Suite 290

Houston, Texas 77063

 

 

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Re: [Histonet] CD3 antibody for FFPE mouse tissue

[Caroline Miller via Histonet]

+1 to the Dako antibody, it is the only one I have ever found to work (but
I have never used them on the automated staining systems, only old-skool by
hand or sequenza)

mills

On Tue, Jun 21, 2016 at 8:19 AM, Elizabeth Chlipala via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Brett
>
> Dako's rabbit anti human CD3 cross reacts with mouse, or where you looking
> for a CD3 that only detects mouse?
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 881-0763 cell
> (303) 682-9060 fax
> l...@premierlab.com
>
> Ship to address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
> 
> From: Connolly, Brett M via Histonet [histonet@lists.utsouthwestern.edu]
> Sent: Tuesday, June 21, 2016 7:59 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] CD3 antibody for FFPE mouse tissue
>
> Hi all,
>
> Looking for recommendation for an anti-mouse CD3 antibody for FFPE
> sections.
>
> I have some old Histonet messages referring to a Neomarkers RB-360 ab
> which I found through Thermo, but I would like to hear about more recent
> experiences/recommendations
>
> Thanks as always,
> Brett
>
> Brett M. Connolly, Ph.D.
> Prin. Scientist,
> Translational Biomarkers - Imaging
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> brett_conno...@merck.com
> T- 215-652-2501
> F- 215-993-6803
>
> Notice:  This e-mail message, together with any attachments, contains
> information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
> New Jersey, USA 07033), and/or its affiliates Direct contact information
> for affiliates is available at
> http://www.merck.com/contact/contacts.html) that may be confidential,
> proprietary copyrighted and/or legally privileged. It is intended solely
> for the use of the individual or entity named on this message. If you are
> not the intended recipient, and have received this message in error,
> please notify us immediately by reply e-mail and then delete it from
> your system.
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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Re: [Histonet] HT Certification

[Jay Lundgren via Histonet]

  Maybe someone can quote CLIA chapter and verse, but, in my understanding,
only registered HTs or HTLs are supposed to be doing embedding, cutting,
special stains and IHC unsupervised.  I think the regulation says
non-registered personnel (trainees) can perform these duties only under
direct supervision.  It all depends on how you define direct supervision.


 To me, direct supervision means someone standing over your shoulder.
However, it has been explained to me that as long as the people working are
being trained toward the exam, it is the responsibility of the Medical
Director of the lab to make sure they are doing the work adequately.  So
most places interpret this as, "Come get me if you have any questions."


  I run into unregistered, OJT techs all the time.   And some of them are
good techs.  And some people get upset when this subject comes up on
Histonet, because they feel like they do the job just as well as someone
who graduated from an NAACLS training program and has passed the test.


  As you said, this is becoming more and more common, and in my opinion, is
the main reason wages are still low, which in turn is the reason why there
is a chronic shortage of histotechs.


  I think you should pay the unregistered trainees minimum wage, sign a
contract stating they must pass the test within 18 months to continue
working, then raise their pay to $30./hr when they do (at least, I don't
know the cost of living in Springfield).  Carrot AND stick.  5% is peanuts,
it won't motivate anyone.  But that's just my opinion.

 Sincerely,

 Jay A. Lundgren, M.S., HTL
(ASCP)



On Tue, Jun 21, 2016 at 12:57 PM, Morken, Timothy via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> I guess one question is, how did they get the impression they would get
> the 5%? That is not something that is normally written into the job
> description, but should be documented in a job offer, or it is made clear
> in some policy that as they gain competency, and the lab organization
> accommodates it,  they will move up the ladder. .  Was that written in or
> verbally given?
>
> The key is something you already mentioned: job classification. To move to
> a higher classification they need to learn the tasks for that
> classification. They need to do the tasks of that job while training so are
> technically doing the work. However, the difference is that while training
> they are under close supervision. Once they pass competency to do the work
> they can work with general supervision. And they get the raise. So if they
> start at entry level that does not require HT, the next level up does
> require it and pays more. That gives incentive to get the certification.
>
> This pertains to any position. Only those at a certain classification are
> allowed to do the work of that classification under general supervision.
> So, once they pass that competency you reclassify them to a higher
> category.  We have histotech 1, 2, 3, 4. 1 and 2 are bench techs doing
> routine work. 3 is a senior tech who can do test development and validation
> and write and edit SOP's. 4 is a Lead tech and expected to supervise a few
> people and organize daily work. Supervisor is above that. Once I am sure a
> person can do 3 work, and I need a 3-level tech, I will reclassify them.
> They get more responsibility and more pay. That is just the fair way to do
> it.
>
> You decide what the jobs tasks are in those classifications. So you can
> tell HR that level 1 an 2 do XXX and level 3 does XXX plus YYY.
>
> We don't make everyone a 3 just because they have been here a long time or
> can do certain things. We limit the number we have (you need a certain
> number of people who do the basic work!). If I have someone who is capable
> of being a 3, but cannot accommodate them in the organization, I will give
> them special short term projects, or find some way to let them do a bit
> more. However some may leave if they can find another  job that will give
> them what they want. That is just the way it is sometimes.
>
>
> Tim
>
>
> -Original Message-
> From: Vickroy, James via Histonet [mailto:
> histonet@lists.utsouthwestern.edu]
> Sent: Tuesday, June 21, 2016 12:28 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] HT Certification
>
> I just got through meeting with HR regarding a salary incentive for
> employees that successfully pass their HT certification.   All of us are
> aware that many histology labs have employees that are not certified.   We
> are a small clinic lab that was set up about a year and a half ago.  When
> we first set up the lab we were able to bring three qualified HT's from
> another lab locally.   The lab has continued to grow since then and now we
> have 4 other staff members that are performing HT tasks including
> microtomy, H staining, and  grossing (they all have BS degrees).  They
> were hired believing that once 

Re: [Histonet] Histonet Digest, Vol 151, Issue 18

[Jo-Ann Bader, Ms. via Histonet]

We are happy the ABCAM CD3


Jo-Ann Bader
Histology Co-Ordinator
Goodman Cancer Centre
McGill University 1160 Pine Ave. W - Rm 312
Montreal, QC, Canada
H3G 1Y6
Tel: 514-398-8270

From: histonet-requ...@lists.utsouthwestern.edu 

Sent: June 21, 2016 12:00:02 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 151, Issue 18

Send Histonet mailing list submissions to
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When replying, please edit your Subject line so it is more specific
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Today's Topics:

   1. CD3 antibody for FFPE mouse tissue (Connolly, Brett M)
   2. Re: CD3 antibody for FFPE mouse tissue (Elizabeth Chlipala)
   3. Re: CD3 antibody for FFPE mouse tissue (Connolly, Brett M)


--

Message: 1
Date: Tue, 21 Jun 2016 09:59:58 -0400
From: "Connolly, Brett M" 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] CD3 antibody for FFPE mouse tissue
Message-ID:

Content-Type: text/plain; charset="us-ascii"

Hi all,

Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections.

I have some old Histonet messages referring to a Neomarkers RB-360 ab which I 
found through Thermo, but I would like to hear about more recent 
experiences/recommendations

Thanks as always,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.


--

Message: 2
Date: Tue, 21 Jun 2016 09:19:49 -0600
From: Elizabeth Chlipala 
To: "Connolly, Brett M" ,
"histonet@lists.utsouthwestern.edu"

Subject: Re: [Histonet] CD3 antibody for FFPE mouse tissue
Message-ID:
<14E2C6176416974295479C64A11CB9AE02BEC711583D@SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"

Brett

Dako's rabbit anti human CD3 cross reacts with mouse, or where you looking for 
a CD3 that only detects mouse?

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
l...@premierlab.com

Ship to address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

From: Connolly, Brett M via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Tuesday, June 21, 2016 7:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD3 antibody for FFPE mouse tissue

Hi all,

Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections.

I have some old Histonet messages referring to a Neomarkers RB-360 ab which I 
found through Thermo, but I would like to hear about more recent 
experiences/recommendations

Thanks as always,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.

Re: [Histonet] HT Certification

[Morken, Timothy via Histonet]

I guess one question is, how did they get the impression they would get the 5%? 
That is not something that is normally written into the job description, but 
should be documented in a job offer, or it is made clear in some policy that as 
they gain competency, and the lab organization accommodates it,  they will move 
up the ladder. .  Was that written in or verbally given?

The key is something you already mentioned: job classification. To move to a 
higher classification they need to learn the tasks for that classification. 
They need to do the tasks of that job while training so are technically doing 
the work. However, the difference is that while training they are under close 
supervision. Once they pass competency to do the work they can work with 
general supervision. And they get the raise. So if they start at entry level 
that does not require HT, the next level up does require it and pays more. That 
gives incentive to get the certification.

This pertains to any position. Only those at a certain classification are 
allowed to do the work of that classification under general supervision. So, 
once they pass that competency you reclassify them to a higher category.  We 
have histotech 1, 2, 3, 4. 1 and 2 are bench techs doing routine work. 3 is a 
senior tech who can do test development and validation and write and edit 
SOP's. 4 is a Lead tech and expected to supervise a few people and organize 
daily work. Supervisor is above that. Once I am sure a person can do 3 work, 
and I need a 3-level tech, I will reclassify them. They get more responsibility 
and more pay. That is just the fair way to do it. 

You decide what the jobs tasks are in those classifications. So you can tell HR 
that level 1 an 2 do XXX and level 3 does XXX plus YYY. 

We don't make everyone a 3 just because they have been here a long time or can 
do certain things. We limit the number we have (you need a certain number of 
people who do the basic work!). If I have someone who is capable of being a 3, 
but cannot accommodate them in the organization, I will give them special short 
term projects, or find some way to let them do a bit more. However some may 
leave if they can find another  job that will give them what they want. That is 
just the way it is sometimes. 


Tim


-Original Message-
From: Vickroy, James via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Tuesday, June 21, 2016 12:28 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] HT Certification

I just got through meeting with HR regarding a salary incentive for employees 
that successfully pass their HT certification.   All of us are aware that many 
histology labs have employees that are not certified.   We are a small clinic 
lab that was set up about a year and a half ago.  When we first set up the lab 
we were able to bring three qualified HT's from another lab locally.   The lab 
has continued to grow since then and now we have 4 other staff members that are 
performing HT tasks including microtomy, H staining, and  grossing (they all 
have BS degrees).  They were hired believing that once they were eligible to 
take the HT certification that they would take the test and if they passed they 
would get a 5% salary adjustment.  However the incentive or 5% increase was not 
written into the job description.

Currently the clinic purchases the study materials (self-instruction program) 
for the staff but we use very little work time for instructional purposes.  The 
staff are expected to study on their own, pay for the exam, and take the test.
If they do not pass the test in the period of one year after completing the  
on-the-job training then technically we could tell them they are no longer 
employed.  However where would that leave us given we couldn't find any 
additional certified HT's to hire in the first place.  We would have to start 
over again with another untrained BS graduate.

One of the question I was asked in the HR meeting was, "What duties can a 
certified HT do that a non-certified technician cannot?".Since all of our 
staff have BS degrees in biology and have all received gross training (90 days) 
I wasn't sure there was anything else that they couldn't do in the lab that 
only a certified HT could do.   I wish there were many duties.   I am afraid 
that if we don't have some certification requirements then in a few years we 
will have very few HT's except those wanting to be supervisory.We are CAP 
certified.Is anybody aware of certain HT duties that can and should only be 
done by a certified HT or HTL?  I know the high complexity requirement in 
grossing but this is based upon 90 days of training and a set number of science 
courses (biology and chemistry), and not certification.

I know that some institutions have handled the incentive to take the HT 
certification by hiring new staff as HT trainees  and then if they passed the 
HT certification they move into another job class which has a 

[Histonet] Thanks! CD3 antibody for mouse IHC

[Connolly, Brett M via Histonet]

Thanks to all who replied to my CD3 query! The responses were just what I was 
hoping for.

Best regards,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
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[Histonet] HT Certification

[Vickroy, James via Histonet]

I just got through meeting with HR regarding a salary incentive for employees 
that successfully pass their HT certification.   All of us are aware that many 
histology labs have employees that are not certified.   We are a small clinic 
lab that was set up about a year and a half ago.  When we first set up the lab 
we were able to bring three qualified HT's from another lab locally.   The lab 
has continued to grow since then and now we have 4 other staff members that are 
performing HT tasks including microtomy, H staining, and  grossing (they all 
have BS degrees).  They were hired believing that once they were eligible to 
take the HT certification that they would take the test and if they passed they 
would get a 5% salary adjustment.  However the incentive or 5% increase was not 
written into the job description.

Currently the clinic purchases the study materials (self-instruction program) 
for the staff but we use very little work time for instructional purposes.  The 
staff are expected to study on their own, pay for the exam, and take the test.
If they do not pass the test in the period of one year after completing the  
on-the-job training then technically we could tell them they are no longer 
employed.  However where would that leave us given we couldn't find any 
additional certified HT's to hire in the first place.  We would have to start 
over again with another untrained BS graduate.

One of the question I was asked in the HR meeting was, "What duties can a 
certified HT do that a non-certified technician cannot?".Since all of our 
staff have BS degrees in biology and have all received gross training (90 days) 
I wasn't sure there was anything else that they couldn't do in the lab that 
only a certified HT could do.   I wish there were many duties.   I am afraid 
that if we don't have some certification requirements then in a few years we 
will have very few HT's except those wanting to be supervisory.We are CAP 
certified.Is anybody aware of certain HT duties that can and should only be 
done by a certified HT or HTL?  I know the high complexity requirement in 
grossing but this is based upon 90 days of training and a set number of science 
courses (biology and chemistry), and not certification.

I know that some institutions have handled the incentive to take the HT 
certification by hiring new staff as HT trainees  and then if they passed the 
HT certification they move into another job class which has a higher salary 
range.  This is an option that may be done in the future here but unfortunately 
that was not set up initially here at the clinic.   I also know of institutions 
that have discouraged BS degree staff from taking the HT certification exam  
thinking that if they become certified they will find a job elsewhere.

So I am trying to list the advantages of staff becoming certified.  The clinic 
in particular wants to know what they get for the 5% increase if someone passes 
the certification.   Any ideas how to respond?

Jim

Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois  62703
Office:  217-528-7541, Ext. 15121
Email:  jvick...@springfieldclinic.com



This electronic message contains information from Springfield Clinic, LLP that 
may be confidential, privileged, and/or sensitive. This information is intended 
for the use of the individual(s) or entity(ies) named above. If you are not the 
intended recipient, be aware that disclosure, copying, distribution, or action 
taken on the contents of this information is strictly prohibited. If you have 
received this electronic message in error, please notify the sender 
immediately, by electronic mail, so that arrangements may be made for the 
retrieval of this electronic message. Thank you.
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[Histonet] CD3 antibody for mouse FFPE

[Erickson, Jamie E via Histonet]

Hi Brett,
We run the Leica BOND RX here and we love the CD3 from Thermo  CD3 (Clone SP7)  
Rabbit Monoclonal Antibody
Cat. #RM-9107-S0, -S1, or -S (0.1ml, 0.5ml, or 1.0ml Supernatant).
We use the H2-20 retrieval which is HIER (EDTA) and it works on human , mouse 
and Rat.
It is a Supernatant so you'll have to call for the ~ concentration it is 
~160ug/ml and works at 0.8ug/ml. With the anti-rabbit polymer detection it 
looks great and the incubated time we use is only 15min.  Another good thing is 
we do not need to use a protein block which saves time.
It looks great as a double with  IBA-1 Alk phos and CD3 black (biocare Deep 
space black) and a light methyl green counter stain.

Hope that helps,
Jamie Erickson, MS
Scientist
AbbVie Bioresearch Center, Inc.
100 Research Drive
Worcester, MA 01605
Ph: 508-688-3134

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Re: [Histonet] CD3 antibody for FFPE mouse tissue

[Claire Weston via Histonet]

Hi Brett,

The anti-CD3 antibody from Abcam #ab5690 works really well in FFPE tissue and 
recognizes mouse.  We have a lot of success using that.

Hope that helps!

Claire

Claire Weston, PhD
Founder & CEO
Reveal Biosciences
revealbio.com   Tel. 858 274 3663

> 
> 
> Hi all,
> 
> Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections.
> 
> I have some old Histonet messages referring to a Neomarkers RB-360 ab which I 
> found through Thermo, but I would like to hear about more recent 
> experiences/recommendations
> 
> Thanks as always,
> Brett
> 
> Brett M. Connolly, Ph.D.
> Prin. Scientist,
> Translational Biomarkers - Imaging
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> brett_conno...@merck.com
> T- 215-652-2501
> F- 215-993-6803
> 
> Notice:  This e-mail message, together with any attachments, contains
> information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
> New Jersey, USA 07033), and/or its affiliates Direct contact information
> for affiliates is available at 
> http://www.merck.com/contact/contacts.html) that may be confidential,
> proprietary copyrighted and/or legally privileged. It is intended solely
> for the use of the individual or entity named on this message. If you are
> not the intended recipient, and have received this message in error,
> please notify us immediately by reply e-mail and then delete it from 
> your system.
> 
> 
> -
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[Histonet] Post with a Prize (see below or disregard)

[Lester Raff MD via Histonet]

HI All:

Although I no longer post blog posts here, this one has a contest with a $25 
gift card prize to the winner, so I am making an exception. Good luck!


http://www.chicagonow.com/downsize-maybe/2016/06/easy-tuesday-music-trivia-with-a-prize/


Best regards,

Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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Re: [Histonet] CD3 antibody for FFPE mouse tissue

[Katy Milne via Histonet]

Spring Bioscience's SP7 clone works really well for mouse (and human) and is 
widely available from a number of companies.  We use it all the time.

Katy

Message: 1
Date: Tue, 21 Jun 2016 09:59:58 -0400
From: "Connolly, Brett M" 
To: "histonet@lists.utsouthwestern.edu"

Subject: [Histonet] CD3 antibody for FFPE mouse tissue
Message-ID:

Content-Type: text/plain; charset="us-ascii"

Hi all,

Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections.

I have some old Histonet messages referring to a Neomarkers RB-360 ab which I 
found through Thermo, but I would like to hear about more recent 
experiences/recommendations

Thanks as always,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803



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Re: [Histonet] CD3 antibody for FFPE mouse tissue

[Colleen Forster via Histonet]

Brett,

I use the rabbit monoclonal CD3 from Thermo (it was a Lab Vision antibody)
with great and consistent results!

The hardest part, actually getting the antibody to me\jeez they have
problems with orders sometimes...

Here is the information for mine:

CD3 (SP7) - cat #RM-9107-S1

I use it at 1:400, citrate retrieval, 1 hour primary incubation. I use the
Rabbit on Rodent polymer from Biocare.

Respectfully,
'
Colleen Forster

On Tue, Jun 21, 2016 at 10:53 AM, Connolly, Brett M via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Liz,
> As long as it detects mouse T-cells it's OK. Unfortunately we have a
> problem getting Dako products through our ordering system.
> Brett
>
> -Original Message-
> From: Elizabeth Chlipala [mailto:l...@premierlab.com]
> Sent: Tuesday, June 21, 2016 11:20 AM
> To: Connolly, Brett M; histonet@lists.utsouthwestern.edu
> Subject: RE: [Histonet] CD3 antibody for FFPE mouse tissue
>
> Brett
>
> Dako's rabbit anti human CD3 cross reacts with mouse, or where you looking
> for a CD3 that only detects mouse?
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 881-0763 cell
> (303) 682-9060 fax
> l...@premierlab.com
>
> Ship to address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
> 
> From: Connolly, Brett M via Histonet [histonet@lists.utsouthwestern.edu]
> Sent: Tuesday, June 21, 2016 7:59 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] CD3 antibody for FFPE mouse tissue
>
> Hi all,
>
> Looking for recommendation for an anti-mouse CD3 antibody for FFPE
> sections.
>
> I have some old Histonet messages referring to a Neomarkers RB-360 ab
> which I found through Thermo, but I would like to hear about more recent
> experiences/recommendations
>
> Thanks as always,
> Brett
>
> Brett M. Connolly, Ph.D.
> Prin. Scientist,
> Translational Biomarkers - Imaging
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> brett_conno...@merck.com
> T- 215-652-2501
> F- 215-993-6803
>
> Notice:  This e-mail message, together with any attachments, contains
> information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
> New Jersey, USA 07033), and/or its affiliates Direct contact information
> for affiliates is available at
> http://www.merck.com/contact/contacts.html) that may be confidential,
> proprietary copyrighted and/or legally privileged. It is intended solely
> for the use of the individual or entity named on this message. If you are
> not the intended recipient, and have received this message in error,
> please notify us immediately by reply e-mail and then delete it from
> your system.
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> Notice:  This e-mail message, together with any attachments, contains
> information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
> New Jersey, USA 07033), and/or its affiliates Direct contact information
> for affiliates is available at
> http://www.merck.com/contact/contacts.html) that may be confidential,
> proprietary copyrighted and/or legally privileged. It is intended solely
> for the use of the individual or entity named on this message. If you are
> not the intended recipient, and have received this message in error,
> please notify us immediately by reply e-mail and then delete it from
> your system.
>
>
> ___
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>
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[Histonet] Histology Laboratory Manager opportunity in Nashville, TN

[Barbara Siegel via Histonet]

Our client is a fast-paced, large volume clinical laboratory seeking
Day-Shift Histology Lab Manager in the Nashville, TN area.  This individual
will directly oversee 2 supervisors (1 day / 1 night) & will be responsible
for all operations / staff of ~22 employees on 3 shifts.   Focus will be on
day-to-day operations, developing mechanisms to monitor, & improve specimen
processing quality & report turnaround times, & maintaining good
relationships with customers, physicians, & colleagues.  The manager will
perform some routine histology tasks of embedding, cutting, IHC's, special
stains as needed, depending upon staff & work volume.   

 

Accountabilities will include:

 

*   Support Client's mission, vision, goals & management decisions.
Provide leadership & manage assigned department, solve problems, develop
procedures, & conduct / attend meetings. 
*   Continuously monitor & evaluate histology operations & workflow,
making changes as needed to assure optimal quality, efficiency,
productivity, & turnaround times.   Maintain required departmental
statistical records & reports.
*   Communicate with clients, marketing, department supervisors,
pathologists, management, & administration regarding laboratory issues.
*   Manage employee hiring process including developing / updating job
descriptions in conjunction with the Human Resources, developing performance
expectations, identifying essential functions & knowledge, abilities
required for applicable positions. 
*   Manage employee performance by coaching, counseling, motivating, &
evaluating employees on a continual basis. Implement disciplinary action as
needed & in consultation with Human Resources. Oversee departmental
training.
*   Ensure effective employee relations by sustaining an ethical,
non-discriminatory & safe work environment & establishing effective
communication lines & methods. Identify & solve employee problems, manage
conflict & respond to grievances as needed. 
*   Establish & execute departmental long & short-term goals. Assist in
the strategic development of the laboratory services. 
*   Achieve budget profitability objectives through controlling costs
and/or increasing market share by effectively utilizing personnel &
resources.
*   Maintain appropriate standards to meet all licensing & regulatory
requirements & uphold company policies & integrity.
*   Perform embedding, microtomy, IHC, & Special stains as needed.
*   Exercise all laboratory safety precautions & adhere to lab
procedures as stated in procedure manuals.
*   Perform all job responsibilities in alignment with the industry's
best security practices & regulatory guidelines to protect the
confidentiality, integrity, & availability of protected health information &
other sensitive company data.
*   Stay up-to-date & abide by the Corporate Compliance Program & all
corporate policies, including the Privacy & Security policies. 

 

Required for this position will be solid supervisory/management expertise in
a similar high volume lab with 5+ year prior exp as a histotechnician.
Appropriate educational & licensure credentials are needed including ASCP
certification, maintenance of continuing education & ability to obtain TN
lab license.

 

This highly-visible opportunity offers competitive salary (to be discussed),
bonus potential & relocation assistance.  Please contact David King at
 biolabcare...@aol.com for more information.

 

David King

Career Studio Biotechnology Div

biolabcare...@aol.com 

561-738-6363

www.linkedin.com/in/biotechnologyhires/

 

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Re: [Histonet] CD3 antibody for FFPE mouse tissue

[Elizabeth Chlipala via Histonet]

Brett

Dako's rabbit anti human CD3 cross reacts with mouse, or where you looking for 
a CD3 that only detects mouse?

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
l...@premierlab.com

Ship to address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

From: Connolly, Brett M via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Tuesday, June 21, 2016 7:59 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CD3 antibody for FFPE mouse tissue

Hi all,

Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections.

I have some old Histonet messages referring to a Neomarkers RB-360 ab which I 
found through Thermo, but I would like to hear about more recent 
experiences/recommendations

Thanks as always,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from
your system.
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[Histonet] CD3 antibody for FFPE mouse tissue

[Connolly, Brett M via Histonet]

Hi all,

Looking for recommendation for an anti-mouse CD3 antibody for FFPE sections.

I have some old Histonet messages referring to a Neomarkers RB-360 ab which I 
found through Thermo, but I would like to hear about more recent 
experiences/recommendations

Thanks as always,
Brett

Brett M. Connolly, Ph.D.
Prin. Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_conno...@merck.com
T- 215-652-2501
F- 215-993-6803

Notice:  This e-mail message, together with any attachments, contains
information of Merck & Co., Inc. (2000 Galloping Hill Road, Kenilworth,
New Jersey, USA 07033), and/or its affiliates Direct contact information
for affiliates is available at 
http://www.merck.com/contact/contacts.html) that may be confidential,
proprietary copyrighted and/or legally privileged. It is intended solely
for the use of the individual or entity named on this message. If you are
not the intended recipient, and have received this message in error,
please notify us immediately by reply e-mail and then delete it from 
your system.
___
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