Re: [Histonet] MITF

[Eddie Martin via Histonet]

Hi Kathy,

The Journal of Investigative Dermatology had an article in their December 2011 
Volume 131 issue 12 on Human Cutaneous Melanomas lacking MITF and melanocyte 
differentiation. According to the article, Melanomas could be MITF and Melan-A 
negative, if the Melanomas were TAM tyrosine kinase receptor positive. In this 
particular study, 38% of the total Cutaneous Melanomas were Axl+ (member of 
TAM), and none of the Axl+ were MITF and Melan-A positive.

That being said, it may explain why some of your cutaneous melanomas are not 
MITF positive.  Known internal control tissue for MITF includes epithelioid 
tissue, mast cells, reactive histiocytes and osteoclasts stain intensely for 
MITF.  A nevus would also be an ideal control tissue, as would any other 
perivascular epithelioid tumor.

Best,
Eddie Martin, HTL, HT(ASCP), QIHC
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[Histonet] Why is Auromine heated in Histology, but not in Microbiology

[Eddie Martin via Histonet]

Hi Angie, 

The simplest answer, would be to save time.  Whether heated, or microwaved at 
80 power for 45 seconds, would be quicker than flooding the slide and letting 
stand for extended periods of time.
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[Histonet] CWD testing

[Johnson, Carole via Histonet]

I would like to hear from people who are doing CWD testing on biopsies (tonsil 
or rectal).  Are you placing a disclaimer on results, and if so do you include 
the number of follicles present in the specimen?  You can answer privately at:  
cjohn...@nmda.nmsu.edu  Thanks for your input

Carole L. Johnson, HT(ASCP)cm, QIHC

New Mexico Department of Agriculture
Veterinary Diagnositc Services
1101 Camino de Salud, NE
Albuquerque, NM 87101
505.383.9299




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[Histonet] Samples not staining with Hematoxylin or primary

[Anna Huntley Coffey via Histonet]

Thanks so much to everyone who responded! A couple people have suggested
that this issue may be due to a disruption to the charge on the slide (due
to high temp baking, water bath additives, etc) which causes a hydrophobic
barrier to form and prevents reagent from reaching the tissue. This sounds
very convincing to me, but we have found that repeated antigen retrieval
using a pressure cooker (our standard retrieval for this particular
primary) has allowed 2 of the 3 slides to stain with both DAB and
Hematoxylin. Would repeated retrieval be able to reverse the slide charge
back to normal?


I'm having an issue with some recent samples and was wondering if anyone
else has experienced the same thing...I stained some samples with a
previously optimized protocol for anti-human Granzyme B on the Ventana
Benchmark and about 1/3 of my samples did not stain at all with either
GranzB or Hematoxylin. My controls and samples that did stain positive for
GranzB look fine and the machine has been recently serviced and working
well with other runs. I'd really appreciate your input if you have any
advice or suggestions on what could have gone wrong.

Thanks,
Anna
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[Histonet] MITF

[Baldwin, Kathy via Histonet]

Hi Histonetters
Was wondering if anyone has had any experience with MITF what controls would 
you use I have a fairly new pathologist that wants us to use Nevus' instead on 
melanoma and half the cases are staining and the other half are not what is 
your experience?  Any help would be greatly appreciated!

Thanks
S. Kathy Baldwin
Histology/Cytology Supervisor
Memorial Hospital and Health Care Center
800 West 9th St.
Jasper, Indiana  47546
Office 812-996-0210
Fax 812-996-0232
Cell 812-887-3357





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Re: [Histonet] Media Lab

[J B via Histonet]

Thank you for the feedback. Was it hard or time consuming to upload all
procedures?   I will only have a few weeks before my CAP window starts.

Thank you,

JB

On Wed, Jul 6, 2016, 11:26 AM Lester Raff MD via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

>
>
> We have been using MediaLab for several years, beginning with their online
> courses and progressing to Document Control and InspectionProof. We are
> awaiting our first CAP inspection in which I expect the inspectors to be
> able to get virtually all the information they need from our MediaLab site.
> Also, customer support is great!
>
>
>
> It took a bit of time for the lab staff to accept the concept of online
> manuals and SOP's but in the long run it is a big win.
>
>
>
> --
>
>
>
> Latest blog post:
> http://www.chicagonow.com/downsize-maybe/2016/07/after-watching-independence-day-resurgence-i-wonder-could-will-smith-get-me-through-an-apocalypse/
>
>
>
> --
>
> Lester J. Raff, MD MBA
>
> UroPartners
>
> Medical Director Of Laboratory
>
> 2225 Enterprise Dr. Suite 2511
>
> Westchester, Il 60154
>
> Tel: 708-486-0076
>
> Fax: 708-492-0203
>
>
>
> -Original Message-
> From: J B via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Wednesday, July 06, 2016 12:23 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Document control system:
>
>
>
> Does anyone have experience with Media Labs document control system?  I am
> looking for good software for documentation control. Any feedback is
> appreciated.
>
>
>
> Sincerely,
>
>
>
> JB
>
> ___
>
> Histonet mailing list
>
> Histonet@lists.utsouthwestern.edu >
>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> Lester J. Raff, MD MBA
> UroPartners
> Medical Director Of Laboratory
> 2225 Enterprise Dr. Suite 2511
> Westchester, Il 60154
> Tel: 708-486-0076
> Fax: 708-492-0203
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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Re: [Histonet] Media Lab

[Lester Raff MD via Histonet]

We have been using MediaLab for several years, beginning with their online 
courses and progressing to Document Control and InspectionProof. We are 
awaiting our first CAP inspection in which I expect the inspectors to be able 
to get virtually all the information they need from our MediaLab site. Also, 
customer support is great!



It took a bit of time for the lab staff to accept the concept of online manuals 
and SOP's but in the long run it is a big win.



--



Latest blog post: 
http://www.chicagonow.com/downsize-maybe/2016/07/after-watching-independence-day-resurgence-i-wonder-could-will-smith-get-me-through-an-apocalypse/



--

Lester J. Raff, MD MBA

UroPartners

Medical Director Of Laboratory

2225 Enterprise Dr. Suite 2511

Westchester, Il 60154

Tel: 708-486-0076

Fax: 708-492-0203



-Original Message-
From: J B via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, July 06, 2016 12:23 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Document control system:



Does anyone have experience with Media Labs document control system?  I am 
looking for good software for documentation control. Any feedback is 
appreciated.



Sincerely,



JB

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Lester J. Raff, MD MBA
UroPartners
Medical Director Of Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, Il 60154
Tel: 708-486-0076
Fax: 708-492-0203

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Re: [Histonet] Samples not staining with Hematoxylin or primary

[Teri Johnson via Histonet]

Anna wrote:
I'm curious as to why we heat the Auramine Rhodamine when we are staining 
tissue sections but our Micro lab stains at room temp.  It is because "it's 
always been done that way"?
Thanks group,
Angie

Hi Anna,

If your dispensers are working properly, I would bet it's your slides. There 
are known issues with overly hydrophobic slides causing issues on the Ventana 
platform. They recommend you use Plus (+) slides manufactured from Erie 
Scientific. There may be others I am not aware of, but we use these slides for 
all IHC sectioned here and have had no issues on our Ventanas.

Good luck!

Teri Johnson, HT(ASCP)QIHC
Manager Clinical Trial Testing
Genoptix, Inc.
SAN5, Rm. 2005
760.516.5954 (office)
760.516.6201 (fax)






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[Histonet] Document control system:

[J B via Histonet]

Does anyone have experience with Media Labs document control system?  I am
looking for good software for documentation control. Any feedback is
appreciated.

Sincerely,

JB
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[Histonet] IHC antibody/assay recommendations for IL-4, IL-13, IL-4R, and IL-13Ra1

[Anna Huntley Coffey via Histonet]

Just wondering if anyone has had good luck with any of these antibodies
(IL-4, IL-13, IL-4R, and IL-13Ra1) and would be willing to share vendor
info and/or assay conditions they've tested?
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Re: [Histonet] curiosity may kill me

[Rene J Buesa via Histonet]

I always used Auramine at room temperature to identify TB bacilli in tissue 
sections with fluorescence filter, never used heat and the results were as 
expected. Bancroft is the one describing the procedure using heat for 
auramine/rhodamine procedure but auramine alone at room temperature is 
enough.René 

On Tuesday, July 5, 2016 4:24 PM, "Bitting, Angela K. via Histonet" 
 wrote:
 

 I'm curious as to why we heat the Auramine Rhodamine when we are staining 
tissue sections but our Micro lab stains at room temp.  It is because "it's 
always been done that way"?
Thanks group,
Angie






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[Histonet] RELIA Hot Histology Job Alert 7-6-2016 Exciting IHC position in CA

[Pam Barker via Histonet]

Hi Histopeeps!
It’s hump day and a short week what more could you ask for?
How about an exciting new job opportunity?
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relocation assistance and an amazing team to work with.  They are in need of
an ASCP HT/HTL certified histotech with strong cutting skills to work in
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My client is ready to interview and hire for this opportunity.  If this is
the right job for you RELIA can make it happen.  

For more information and the EXACT location please contact Pam Barker at
rel...@earthlink.net or toll free at 866-607-3542 or cell/text 407-353-5070.

RELIA Solutions is the nation's ONLY recruiting firm specializing in the
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To sign up for our free histology careers bulletin please send an email to
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Keywords: Histology, histologist, histotechnician, histotechnologist,
immunohistochemistry, IHC, CA

Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com/PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 






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