Re: [Histonet] TRAP Stain

[Jessica Riggleman via Histonet]

Also if anyone has any photos of slides stained that would be great. We are 
looking for osteoclasts.



_

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

Confidentiality Note:  This email is confidential and intended solely for the 
use of the individual to whom it is addressed. If you are not the intended 
recipient, be advised that you have received this email in error and that any 
use, dissemination, forwarding, printing, or copying of this email is strictly 
prohibited. If you have received this email in error please contact the sender. 
Any views or opinions presented are solely those of the author and do not 
necessarily represent those of Globus Medical, Inc. Although this email and any 
attachments are believed to be free of any virus or other defects which might 
affect any computer or IT system into which they are received, no 
responsibility is accepted by Globus Medical, Inc. for any loss or damage 
arising in any way from the receipt or use thereof.


-Original Message-
From: Jessica Riggleman
Sent: Monday, September 12, 2016 3:01 PM
To: 'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu) 

Subject: TRAP Stain

Can anyone recommend a TRAP (tartrate resistant acid phosphatase) protocol for 
formalin fixed paraffin embedded Rabbit PLF specimens? Or a kit to use?

Thank you,
Jessica
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] TRAP Stain

[Jessica Riggleman via Histonet]

Can anyone recommend a TRAP (tartrate resistant acid phosphatase) protocol for 
formalin fixed paraffin embedded Rabbit PLF specimens? Or a kit to use?

Thank you,
Jessica


_

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

Confidentiality Note:  This email is confidential and intended solely for the 
use of the individual to whom it is addressed. If you are not the intended 
recipient, be advised that you have received this email in error and that any 
use, dissemination, forwarding, printing, or copying of this email is strictly 
prohibited. If you have received this email in error please contact the sender. 
Any views or opinions presented are solely those of the author and do not 
necessarily represent those of Globus Medical, Inc. Although this email and any 
attachments are believed to be free of any virus or other defects which might 
affect any computer or IT system into which they are received, no 
responsibility is accepted by Globus Medical, Inc. for any loss or damage 
arising in any way from the receipt or use thereof.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] The NSH Convention is in Long Beach 9/16-9/21 Hope to See you there!!

[Pam Barker via Histonet]

Hi Histonetters!!
How are you?  As you may know I do the majority of my work via email and
phone so I rarely get a chance to meet people in person.  
That is one of the many reasons I enjoy attending the NSH conferences.  
I wanted to drop you a line and see if you or anyone you work with is
planning on attending the NSH Conference in Long Beach next week? 
 
For more info here is the link: www.histoconvention.org 
 
I would love to have the opportunity to say Hi.
I will be very easy to find because
I am working the registration desk all day
Friday and Saturday and Sunday morning
AND 
The NSH membership booth in the exhibit hall on Sunday afternoon 
AND 
the Florida PR table on Monday  
So please stop by and say Hi!
   
If you aren't attending this year follow the fun on
FACEBOOK, INSTAGRAM and TWITTER.
We will be posting with the hashtag #NSHSC 
 
We will be posting lots of pics and statuses
about all the exciting stuff going on so stay tuned!!
 
Also. If you are not able to attend are there any concerns or issues I can
ask other techs about and report back to you?
 
By the way.
Here is a list of my current openings:
HISTOLOGY SUPERVISORS/MANAGERS:
Director of Laboratory Services - Stockton, CA
Histology Lab Manager - Nashville, TN
Lead Histology Tech - Las Cruces, NM
HISTOTECHNICIANS/HISTOTECHNOLOGISTS:
Histology Tech - Norfolk, VA - 15K sign on bonus plus relo.
Histotech - Manitowoc, WI - learn new skills!  NEW Client!!
Histotech/IHC - Modesto, CA
Histology Tech - Asheville, NC
Histology Tech - Kingsport, TN
 
All of these positions are full time and permanent and offer great salaries
excellent benefits, relocation and/or sign on bonuses.  
For more information please contact me at rel...@earthlink.net  or on my
cell at 407-353-5070 or toll free at 866-607-3542.
 
Hope To See You in Long Beach!!
Have a great day!!
p.s. don't forget: let me know if there is anything I can find out for you
there.
 
Thanks-Pam


Thanks-Pam

Right Place, Right Time, Right Move with RELIA!

Thank You!
 Pam M. Barker
 
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: rel...@earthlink.net 
www.facebook.com  /PamBarkerRELIA
www.linkedin.com/in/reliasolutions
www.twitter.com/pamatrelia 





___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Histotech job openings in Manitowoc, WI

[Brannon Owens via Histonet]

Histotechs needed for full time/permanent job opportunities in Manitowoc, WI.  
Relocation assistance and competitive pay offered - multiple shifts available.  
Send an update resume to bran...@alliedsearchpartners.com for review.

To view a complete list of Allied Search Partners current openings go to:  
http://www.jobs.net/jobs/alliedsearchpartners/en-us/all-jobs/United-States/

Brannon Owens
Recruitment Manager
Allied Search Partners
LinkedIn: http://www.linkedin.com/pub/brannon-owens/28/528/823
http://www.alliedsearchpartners.com
T: 888.388.7571 ext. 106
Direct Line:  407.413.9421
F: 888.388.7572

___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Egg Whites and IHC Staining

[Jessica Riggleman via Histonet]

Thank you Elizabeth. I will try those slides, I have not used them before. Do 
they have in 2x3? Is there a way you can send me your RAM11 protocol?

Thank you,
Jessica



_

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

Confidentiality Note:  This email is confidential and intended solely for the 
use of the individual to whom it is addressed. If you are not the intended 
recipient, be advised that you have received this email in error and that any 
use, dissemination, forwarding, printing, or copying of this email is strictly 
prohibited. If you have received this email in error please contact the sender. 
Any views or opinions presented are solely those of the author and do not 
necessarily represent those of Globus Medical, Inc. Although this email and any 
attachments are believed to be free of any virus or other defects which might 
affect any computer or IT system into which they are received, no 
responsibility is accepted by Globus Medical, Inc. for any loss or damage 
arising in any way from the receipt or use thereof.


-Original Message-
From: Elizabeth Chlipala [mailto:l...@premierlab.com]
Sent: Monday, September 12, 2016 11:53 AM
To: Jessica Riggleman ; 
'histonet@lists.utsouthwestern.edu' (histonet@lists.utsouthwestern.edu) 

Subject: RE: Egg Whites and IHC Staining

Jessica

There are a couple of things that we will try here when we have problems with 
tissue adherence

1.  We utilize a particular brand of plus slides  - trubond 380 - these have 
worked very well in our hands, we purchase them from Newcomer Supply 2.  Post 
fix in 10% NBF for 10 minutes after you run the slides down to water - this 
does not impact the H staining but can impact IHC staining we have seen that 
is may decrease sensitivity so you would need to potentially rework your IHC 
protocol 3.  Our Ram-11 IHC protocol requires HIER we have two different 
retrieval protocols that we have validated for this particular antibody - our 
routine 95C for 30 minutes and then a 70C for 2 hours - we use the longer lower 
temp retrieval when we have problems with tissue adherence, again you may need 
to potentially rework your IHC protocol for the lower temp retrieval.

Hope this helps, good luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box 18592 
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: Jessica Riggleman via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, September 12, 2016 8:05 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Egg Whites and IHC Staining

Hello,

We are cutting large Rabbit PLF formalin fixed paraffin embedded sections.

We have had issues staining H and Goldner’s Trichrome. Egg whites is working 
as a great slide adhesive for these two, but not so much for IHC Staining (RAM 
11 specifically).

Any ideas/suggestions on how to get these sections to adhere? We have tried 
many methods, such as positively charged slides, chrom-alum gel, and so on.

Thank you for all input,
Jessica


_

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

Confidentiality Note:  This email is confidential and intended solely for the 
use of the individual to whom it is addressed. If you are not the intended 
recipient, be advised that you have received this email in error and that any 
use, dissemination, forwarding, printing, or copying of this email is strictly 
prohibited. If you have received this email in error please contact the sender. 
Any views or opinions presented are solely those of the author and do not 
necessarily represent those of Globus Medical, Inc. Although this email and any 
attachments are believed to be free of any virus or other defects which might 
affect any computer or IT system into which they are received, no 
responsibility is accepted by Globus Medical, Inc. for any loss or damage 
arising in any way from the receipt or use thereof.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] patient identifiers

[Kienitz, Kari via Histonet]

Good morning everyone,

Along with an accession number, is a patients intitials an adequate identifier 
on a block/slide?




Kari Kienitz HT, (ASCP)
Histology Laboratory
Gastroenterology-EAST
The Oregon Clinic
 NE 99th Ave
Portland, OR  97220
503.935.8311
kkien...@orclinic.com




CONFIDENTIALITY WARNING: This e-mail and any attachments are for the exclusive 
and confidential use of the intended recipient. If you are not the intended 
recipient, please do not read, distribute or take action in reliance upon this 
missive. If you have received this in error, please notify the sender 
immediately by reply e-mail and delete this message and its attachments from 
your computer system. Thank you
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Egg Whites and IHC Staining

[Elizabeth Chlipala via Histonet]

Jessica

There are a couple of things that we will try here when we have problems with 
tissue adherence

1.  We utilize a particular brand of plus slides  - trubond 380 - these have 
worked very well in our hands, we purchase them from Newcomer Supply
2.  Post fix in 10% NBF for 10 minutes after you run the slides down to water - 
this does not impact the H staining but can impact IHC staining we have seen 
that is may decrease sensitivity so you would need to potentially rework your 
IHC protocol
3.  Our Ram-11 IHC protocol requires HIER we have two different retrieval 
protocols that we have validated for this particular antibody - our routine 95C 
for 30 minutes and then a 70C for 2 hours - we use the longer lower temp 
retrieval when we have problems with tissue adherence, again you may need to 
potentially rework your IHC protocol for the lower temp retrieval.

Hope this helps, good luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-Original Message-
From: Jessica Riggleman via Histonet [mailto:histonet@lists.utsouthwestern.edu] 
Sent: Monday, September 12, 2016 8:05 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Egg Whites and IHC Staining

Hello,

We are cutting large Rabbit PLF formalin fixed paraffin embedded sections.

We have had issues staining H and Goldner’s Trichrome. Egg whites is working 
as a great slide adhesive for these two, but not so much for IHC Staining (RAM 
11 specifically).

Any ideas/suggestions on how to get these sections to adhere? We have tried 
many methods, such as positively charged slides, chrom-alum gel, and so on.

Thank you for all input,
Jessica


_

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

Confidentiality Note:  This email is confidential and intended solely for the 
use of the individual to whom it is addressed. If you are not the intended 
recipient, be advised that you have received this email in error and that any 
use, dissemination, forwarding, printing, or copying of this email is strictly 
prohibited. If you have received this email in error please contact the sender. 
Any views or opinions presented are solely those of the author and do not 
necessarily represent those of Globus Medical, Inc. Although this email and any 
attachments are believed to be free of any virus or other defects which might 
affect any computer or IT system into which they are received, no 
responsibility is accepted by Globus Medical, Inc. for any loss or damage 
arising in any way from the receipt or use thereof.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Job opening

[Armstrong, Karoleigh T via Histonet]

Carl, you are so right, I do mean an H.T.L., by Friday afternoon, mommy's brain 
is cooked! Thanks.

Jay, I really am looking for full time. After over 30 years, I am getting ready 
to retire, in about a year or two, and I want to train someone to take my place 
as Supervisor. This is a great Lab, and the people are fabulous.

Thanks for the interest>

KK


--
Disclaimer: This electronic message may contain information that is 
Proprietary, Confidential, or legally privileged or protected. It is intended 
only for the use of the individual(s) and entity named in the message. If you 
are not an intended recipient of this message, please notify the sender 
immediately and delete the material from your computer. Do not deliver, 
distribute or copy this message and do not disclose its contents or take any 
action in reliance on the information it contains.
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Egg Whites and IHC Staining

[Rene J Buesa via Histonet]

Egg white (in Mueller's albumin) will always produce a "shadowy" staining with 
IH procedures y a dark background with IHC procedures.I suggest you use pork 
gelatin dissolves in the water of the water bath, use (+) charged slides and 
increase the drying time in the oven.Also sectioning those large pieces as thin 
as possible will help.René 

On Monday, September 12, 2016 10:25 AM, Jessica Riggleman via Histonet 
 wrote:
 

 Hello,

We are cutting large Rabbit PLF formalin fixed paraffin embedded sections.

We have had issues staining H and Goldner’s Trichrome. Egg whites is working 
as a great slide adhesive for these two, but not so much for IHC Staining (RAM 
11 specifically).

Any ideas/suggestions on how to get these sections to adhere? We have tried 
many methods, such as positively charged slides, chrom-alum gel, and so on.

Thank you for all input,
Jessica


_

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

Confidentiality Note:  This email is confidential and intended solely for the 
use of the individual to whom it is addressed. If you are not the intended 
recipient, be advised that you have received this email in error and that any 
use, dissemination, forwarding, printing, or copying of this email is strictly 
prohibited. If you have received this email in error please contact the sender. 
Any views or opinions presented are solely those of the author and do not 
necessarily represent those of Globus Medical, Inc. Although this email and any 
attachments are believed to be free of any virus or other defects which might 
affect any computer or IT system into which they are received, no 
responsibility is accepted by Globus Medical, Inc. for any loss or damage 
arising in any way from the receipt or use thereof.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


   
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Egg Whites and IHC Staining

[Mca Werdler via Histonet]

I am also very interested in this! please let me know a good adhesive for
IHC

2016-09-12 9:05 GMT-05:00 Jessica Riggleman via Histonet <
histonet@lists.utsouthwestern.edu>:

> Hello,
>
> We are cutting large Rabbit PLF formalin fixed paraffin embedded sections.
>
> We have had issues staining H and Goldner’s Trichrome. Egg whites is
> working as a great slide adhesive for these two, but not so much for IHC
> Staining (RAM 11 specifically).
>
> Any ideas/suggestions on how to get these sections to adhere? We have
> tried many methods, such as positively charged slides, chrom-alum gel, and
> so on.
>
> Thank you for all input,
> Jessica
>
>
> _
>
> Jessica Riggleman | Research Associate
>
> Globus Medical, Inc.
> Valley Forge Business Center
> 2560 General Armistead Avenue | Audubon, PA 19403
> Ph: (610) 930-1800 ext. 2583 | Fax:
>
> Confidentiality Note:  This email is confidential and intended solely for
> the use of the individual to whom it is addressed. If you are not the
> intended recipient, be advised that you have received this email in error
> and that any use, dissemination, forwarding, printing, or copying of this
> email is strictly prohibited. If you have received this email in error
> please contact the sender. Any views or opinions presented are solely those
> of the author and do not necessarily represent those of Globus Medical,
> Inc. Although this email and any attachments are believed to be free of any
> virus or other defects which might affect any computer or IT system into
> which they are received, no responsibility is accepted by Globus Medical,
> Inc. for any loss or damage arising in any way from the receipt or use
> thereof.
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Egg Whites and IHC Staining

[Jessica Riggleman via Histonet]

Hello,

We are cutting large Rabbit PLF formalin fixed paraffin embedded sections.

We have had issues staining H and Goldner’s Trichrome. Egg whites is working 
as a great slide adhesive for these two, but not so much for IHC Staining (RAM 
11 specifically).

Any ideas/suggestions on how to get these sections to adhere? We have tried 
many methods, such as positively charged slides, chrom-alum gel, and so on.

Thank you for all input,
Jessica


_

Jessica Riggleman | Research Associate

Globus Medical, Inc.
Valley Forge Business Center
2560 General Armistead Avenue | Audubon, PA 19403
Ph: (610) 930-1800 ext. 2583 | Fax:

Confidentiality Note:  This email is confidential and intended solely for the 
use of the individual to whom it is addressed. If you are not the intended 
recipient, be advised that you have received this email in error and that any 
use, dissemination, forwarding, printing, or copying of this email is strictly 
prohibited. If you have received this email in error please contact the sender. 
Any views or opinions presented are solely those of the author and do not 
necessarily represent those of Globus Medical, Inc. Although this email and any 
attachments are believed to be free of any virus or other defects which might 
affect any computer or IT system into which they are received, no 
responsibility is accepted by Globus Medical, Inc. for any loss or damage 
arising in any way from the receipt or use thereof.


___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet