[Histonet] Need Suggestions: IHC Slides and Floating Sections + 4% PFA Fixation
Hi All 1) How long vibratom liver sections when cut in ice cold PBS and then stored at 4C in PBS could be used? 2) How long paraffin embedded liver tissues after dewaxing and antigen retrieval stored in PBS at 4C could be used? 3) Whether liver tissues fixed in 4% PFA for more than a month would have any affect on antigen integrity?when antigen of interests (CD68, F4/80, Ly6g, CD4 and CD8) are low in abundance (different time points from 15min to 96hrs of injury)? Has any body encountered such problem before? If such was the case with you, how would have you tackle it? and finally,3) Any body could suggest liver pathology atlas with normal to special stain please? For your opinions and suggestions, thanks in advance. Sincerely Nasir Abbas ---PhD FellowGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan Avenue Science Park Guangzhou, 510530, Guangdong P.R.ChinaCell: +86-1312 8296 614 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Maximow Method
Hello, I was reading a book from the 60s on the anatomy of aging in man and animals and the author mentioned using a hematoxylin-eosin Y-Azure-II stain to show the lymphocytes. Some searching came up with the Maximow Method. The online protocol I found (for bone marrow): https://emsdiasum.com/microscopy/technical/datasheet/26252.aspx mentions the use of Zenker’s or Formalin. In another old book, Putt's Manual of Histochemical Staining Methods, the authors says 10% fomalin, Helly's or Zenker's fluid for fixation. I am definitely not going to start using Zenker's (I get enough grief from the H officer about picric acid). I normally use Davidson's fixative to fix and decalsify my fish (formaldehyde, acetic acid + ethanol). Anyone know if this staining method is compatible with Davidson's fixative? Would this eosin-Y solution be suitable: http://www.sigmaaldrich.com/catalog/product/sigma/ht110316?lang=en=US for preparing the Eosin-Y Azure-II working solution? Thanks -- Tyrone Genade Orange City, Iowa tel: (+1) 712 230 4101 http://tgenade.freeshell.org Romans 6:23: The gift of God is eternal life through Christ Jesus our Lord. To find out how to receive this FREE gift visit http://www.alpha.org. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] Leishmaniasis ctrl
Hi all, Does anyone know of a source for a Leishmaniasis control block or control slides for IHC? Thanks in advance! Erin Martin, Histology Supervisor UCSF Dermatopathology and Oral Pathology Service 1701 Divisadero St, San Francisco, CA 94044 415-353-7248 Confidentiality Notice The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by persons or entities other than the intended recipient is prohibited. If you receive this in error please contact the sender and delete the material from any computer. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] (no subject)
Hello, We looking to bring Kappa and Lambda by CISH on board here in my laboratory. We have Ventana Benchmark Ultra instruments. Can anyone running these tests let me know how long your staining protocol is? I am putting together a report on how this change will impact workflow and turn around times. Thank you all. Melissa Kuhnla Lead Medical Technologist for IHC and FISH testing Regional Laboratory Services Good Samaritan Hospital 631-609-2551 The information in this e-mail, and any attachments therein, is confidential and for use by the intended addressee only. If this message is received by you in error please do not disseminate or read further. Please reply to the sender that you have received the message in error, then delete the message. Although Catholic Health Services of Long Island attempts to sweep e-mail and attachments for viruses, it does not guarantee that either are virus-free and accepts no liability for any damage sustained as a result of viruses. Thank you. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
