[Histonet] Thinprep staining

[Charles Riley via Histonet]

The pathologists at my office keep asking me to fix the thin prep staining.
This is not part of my expertise as I was only given about 10 minutes worth
of cytoprep training in school. If anyone out there can offer any
assistance for the problem it would be greatly appreciated. Here is what
they have said is occurring:

"The Orange G staining is too light and inconsistently distributed across
the slide.

I have also noticed some perinuclear artifacts such as perinuclear
clearing."

Thanks in advance for your help


-- 

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
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Re: [Histonet] Syringe for Lab Vision 720D Stainer Needed! (Walter Benton)

[Ranna Mehta via Histonet]

Hi Sandra,

   Contact Victor Wong

Cell 646-378-9222

serv...@autostainertech.com

He is very good with lab vision platform.


Thanks

Ranna Mehta




On Tue, Jan 31, 2017 at 12:00 PM,  wrote:

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> Today's Topics:
>
>1. Little Rock Day Shift Histotech Recruitment (Angie Laparidis)
>2. Re: Histonet Digest, Vol 158, Issue 22 Breast Specimen
>   (Steve McClain)
>3. Syringe for Lab Vision 720D Stainer Needed! (Sandra Cheasty)
>4. Re: Breast specimens (Bob Richmond)
>5. Re: Syringe for Lab Vision 720D Stainer Needed! (Walter Benton)
>6. Re: Breast specimens (Drinkall, Liz)
>7. job descriptions (Caleri, Kathleen)
>8. CAP Gen 41770 glassware cleaning (Mike Toole)
>
>
> --
>
> Message: 1
> Date: Mon, 30 Jan 2017 13:50:37 -0500
> From: Angie Laparidis 
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Little Rock Day Shift Histotech Recruitment
> Message-ID:
>  mail.gmail.com>
> Content-Type: text/plain; charset=UTF-8
>
> Happy Monday Histonet Users!
>
> I wanted to share a brand new opening with a client of mine in Little Rock,
> AR. It is a hospital setting-with the shifts starting from 4:30 to 6:00 AM
> and are eight hours long. Strong preference for surgpath experience.
>
>
> Great hospital, great location, amazing shift- this position will not be
> open very long! Send your applications to me at an...@ka-recruiting.com
> and
> I look forward to speaking with you!
>
>
> Sincerely,
>
>
> *Angie Laparidis*Healthcare Recruiter
> K.A. Recruiting, Inc.
> 10 Post Office Square, 8th Floor South, Boston, MA, 02109
> W:  617.746-2744 (*please note this is a new number*)
> F: (617) 507-8009
> an...@ka-recruiting.com
>
>
> Our openings are updated daily at www.ka-recruiting.com
>
>
> --
>
> Message: 2
> Date: Mon, 30 Jan 2017 20:14:24 +
> From: Steve McClain 
> To: "histonet@lists.utsouthwestern.edu"
> 
> Subject: Re: [Histonet] Histonet Digest, Vol 158, Issue 22 Breast
> Specimen
> Message-ID:
> 
> Content-Type: text/plain; charset="us-ascii"
>
> In my experience, rushing to process fatty or inadequately fixed specimens
> is a fool's game.
> In my opinion, this problem cannot be solved by the histotechs- it begins
> with the grossers and is one for the pathologists to solve at the grossing
> bench.
>
> Suggestion #1 Do nothing and let the medical director pathologist/ sign it
> out/deal w this individual case.
>
> Suggestion #2  Sometimes a decent section can be obtained after changing
> paraffin.
> [place the blocks in molds and melt the blocks and change to new paraffin-
> let them sit in the embedding center in the new paraffin for 60 minutes.
> Re-embed in new paraffin (2 changes) and then re-embed.]
>
> Suggestion #3 Reprocess these blocks if permitted, recognizing that breast
> markers (if cancerous) may be erroneous.
>
> Suggestion #4 Seek to prevent future occurrences by adjusting behavior at
> the grossing bench.
> a) first ensuring adequate fixation and b) second ensuring adequate
> length/time of processing.
>
>
> Steve A. McClain, MD
> 631 361 4000
>
> What is the best way to handle Breast specimens that were grossed too
> thick and did not process well?  Our medical director does not want us to
> reprocess the tissue but it is almost impossible to get even a remotely
> decent section. If anyone has any other tips please let me know as soon as
> possible
>
> --
>
> Charles Riley HT(ASCP)CM
>
> Histopathology Coordinator/ Mohs**
>
>
>
> --
>
> Message: 3
> Date: Mon, 30 Jan 2017 21:00:59 +
> From: Sandra Cheasty 
> To: "Histonet (histonet@lists.utsouthwestern.edu)"
> 
> Subject: [Histonet] Syringe for Lab Vision 720D Stainer Needed!
> Message-ID:
>  namprd06.prod.outlook.com>
>
> Content-Type: text/plain; CHARSET=US-ASCII
>
> Hello everyone!
> Can someone tell me where I can get a replacement syringe
> for the Lab Vision 720 stainer? It is supposed to be 

Re: [Histonet] Histonet Digest, Vol 158, Issue 22 Breast Specimen

[Rathborne, Toni via Histonet]

There is also a CAP checklist standard that might help you to document these 
cases, without it seeming like you're complaining. Once the offenders have it 
brought to their attention, and know that it is being documented, they may 
start to give more consideration to the sections they're putting through.

**NEW** 07/28/2015
ANP.10038 Tissue Sample Quality Phase II There is a procedure that describes 
the process by which histotechnologists provide feedback to submitting 
pathologists and pathology assistants on the quality of the gross tissue 
sections received for tissue processing.
NOTE: Inadequate fixation, overly thick tissue sections, non-decalcified bone, 
the presence of staples, etc., can lead to poor quality histologic sections 
and/or poor quality special stains/special studies.
This requirement applies to both laboratories that gross tissue and perform all 
processing onsite, as well as laboratories that gross tissue and send it to 
another laboratory for processing, embedding, and sectioning (regardless of the 
outside laboratory's accrediting organization).
Records of such feedback and corrective action taken when problems are 
identified may be incorporated into the laboratory's quality management program.
Evidence of Compliance:
✓ Records of feedback and corrective action for problems identified with tissue 
quality


Toni


-Original Message-
From: Steve McClain via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, January 30, 2017 3:14 PM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Histonet Digest, Vol 158, Issue 22 Breast Specimen


*** This is an External Email ***

In my experience, rushing to process fatty or inadequately fixed specimens is a 
fool's game.
In my opinion, this problem cannot be solved by the histotechs- it begins with 
the grossers and is one for the pathologists to solve at the grossing bench.

Suggestion #1 Do nothing and let the medical director pathologist/ sign it 
out/deal w this individual case.

Suggestion #2  Sometimes a decent section can be obtained after changing 
paraffin.
[place the blocks in molds and melt the blocks and change to new paraffin- let 
them sit in the embedding center in the new paraffin for 60 minutes. Re-embed 
in new paraffin (2 changes) and then re-embed.]

Suggestion #3 Reprocess these blocks if permitted, recognizing that breast 
markers (if cancerous) may be erroneous.

Suggestion #4 Seek to prevent future occurrences by adjusting behavior at the 
grossing bench.
a) first ensuring adequate fixation and b) second ensuring adequate length/time 
of processing.


Steve A. McClain, MD
631 361 4000

What is the best way to handle Breast specimens that were grossed too thick and 
did not process well?  Our medical director does not want us to reprocess the 
tissue but it is almost impossible to get even a remotely decent section. If 
anyone has any other tips please let me know as soon as possible

--

Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs**

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[Histonet] Sample accessioning software

[Judi Ford via Histonet]

Hi Everyone,
Happy Tuesday :).
My group is looking into software which would assign accession numbers, track 
histology specimens, list archive location, track disposition info, hold 
images, ability to attach scientific data, has security and to create reports. 
I would love to hear what anyone may be using or can suggest that I look into.
Thanks so much for any and all responses that come my way. I really appreciate 
it.
Best to all,
Judi Ford
CytomX Therapeutics,
South San Francisco, CA


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[Histonet] CAP Gen 41770 glassware cleaning

[Mike Toole via Histonet]

Jessica,



I am not sure about requirements to use and test for detergent, but the 
following steps are good practices.



Clearing containers should be kept separate from other containers. They need 
only to be wiped out with a paper towel. No water, detergent or bleach.



Alcohols can be cleaned with tap water and a brush only, then given a DI rinse 
to avoid residue. Wipe dry.



Stain containers should also be cleaned only with a brush and tap water. If 
necessary, a small amount of a 10% solution of HCl and 70% OH can be used 
followed by a thorough DI rinsing. If this is not sufficient, then follow with 
10% bleach being sure to rinse thoroughly with DI water and wipe dry.



Chlorine test strips and phosphate test kits can be used to test for bleach and 
detergent residues. Even though detergent is not called for.



Chlorine test strip: 
https://www.indigo.com/test_strips/disinfectants_sanitizers/chlorine_and_iodine/chlorine-test-papers-200ppm-33815.html



API Phosphate Test Kit: 
http://www.apifishcare.com/product.php?id=589#.WIEFOlMrIkI



Mike




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[Histonet] job descriptions

[Caleri, Kathleen via Histonet]

We are currently reviewing job descriptions...so, what tasks do you require of 
your histotechs?  Besides the basic embedding, cutting, and staining (routine 
and special IHC), instrument maintenance...what else are they expected to 
do-what do you consider to be appropriate tasks for entry level techs and for 
seasoned techs? Do they do any grossing-and to what extent? Do they assist with 
autopsies-to what extent? If anyone wants to share their descriptions, please 
email me directly at 
kathleen.cal...@roswellpark.org

I am mostly interested in what NYS labs are doing but would like to hear from 
everyone.  Thanks!


Kate Caleri BS HT(ASCP)
Histology Lab Supervisor
Roswell Park Cancer Institute


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Re: [Histonet] Syringe for Lab Vision 720D Stainer Needed!

[Walter Benton via Histonet]

Check Biocare Medical. They use the same "box" and just place their name on it. 
Biocare calls it the Nemesis.


Walter Benton HT(ASCP)QIHC
Lab Operations Manager
Chesapeake Urology Associates
806 Landmark Drive, Suite 127
Glen Burnie, MD 21061
443-471-5850 (Direct)
410-768-5961 (Lab)
410-768-5965 (Fax)
Chesapeakeurology.com

Voted a Best Place to Work by
Baltimore and Modern Healthcare
Magazines.



-Original Message-
From: Sandra Cheasty via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, January 30, 2017 4:01 PM
To: Histonet (histonet@lists.utsouthwestern.edu) 

Subject: [Histonet] Syringe for Lab Vision 720D Stainer Needed!

Hello everyone!
Can someone tell me where I can get a replacement syringe for 
the Lab Vision 720 stainer? It is supposed to be part # NM014. Thermo Fisher 
has been unable to help me so far.
Thanks!
Sandy

Sandra J. Cheasty, HT (ASCP)
Histology & Necropsy Supervisor
UW-Madison, School of Veterinary Medicine

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