Re: [Histonet] help!!

2017-04-18 Thread Tony Henwood (SCHN) via Histonet 
You can use DAB but the issue of endogenous peroxidase will rear its ugly head.

And I suppose IF being around since 1955, when Mellors first applied the 
technique to renal tissue, it has a long history of diagnostic application that 
is hard to replace.

There are other enzymes that could be used that, not being present in human 
tissue, would not require endogenous enzyme blocking for example glucose 
oxidase. The advantage of this enzyme is that there is no endogenous glucose 
oxidase activity in mammalian tissues.

Weening, J. J., & Jennette, J. C. (2012). Historical milestones in renal 
pathology. Virchows Archiv, 461(1), 3-11.

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

From: Allan Wang [mailto:alla...@gmail.com]
Sent: Tuesday, 18 April 2017 5:51 PM
To: Tony Henwood (SCHN)
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] help!!

Tim and Tony,

Why couldn't DAB be used on frozen sections in your example?

Allan

On Mon, Apr 17, 2017 at 9:03 PM, Tony Henwood (SCHN) via Histonet 
> 
wrote:
Hi Bianca,
Well for most Pathology departments, Immunofluorescence (IF) is used for Renal 
and Skin biopsies; looking for human Immunoglobulin (Igs) deposition on 
basement membranes. The advantage here is not so much the fluorescence, but 
that we use unfixed frozen sections. The buffer rinse before antibody 
application, removes un-bound serum immunoglobulins, leaving any pathological 
bound Igs for the IF antibody to bind to. This gives a clean result.

If one would do IF on formalin-fixed paraffin sections of renal or skin 
biopsies, you would find heavy background due to the fixative cross-linking 
serum Igs to tissue and cells (which would usually be removed by the buffer 
rinse if unfixed frozen sections were used - see above).

IF, apart from being a historic method, also does not suffer from endogenous 
peroxidase that would need to be blocked if peroxidase was used in place of 
fluorescence.



Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

-Original Message-
From: Blanca Lopez via Histonet 
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, 13 April 2017 11:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] help!!
Hello!
I just need a help with a simple question...Is anyone can explain me what is 
the purpose between performing immunohistochemistry and Immunofluorescence?
Thanks  :)

Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: blanca.lo...@utsouthwestern.edu




UT Southwestern


Medical Center



The future of medicine, today.

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[Histonet] PT result forms

2017-04-18 Thread Mighnon Lashus via Histonet 
Does anyone have a PT result form to use when the CAP PT results have not been 
submitted on time?  Would you be willing to share?
Thanks,

Mighnon Lashus, HT (ASCP)
Laboratory Manager

PathGroup
4071 S. Access Rd, Suite 107
Chattanooga, TN 37406
423-493-0207
423-493-0208 fax
mlas...@pathgroup.com

Important Notice: This e-mail is intended for the use of the person to whom it 
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If you are not the intended recipient, any disclosure, copying, distribution, 
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Re: [Histonet] help!!

2017-04-18 Thread Allan Wang via Histonet 
Tim and Tony,

Why couldn't DAB be used on frozen sections in your example?

Allan

On Mon, Apr 17, 2017 at 9:03 PM, Tony Henwood (SCHN) via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hi Bianca,
> Well for most Pathology departments, Immunofluorescence (IF) is used for
> Renal and Skin biopsies; looking for human Immunoglobulin (Igs) deposition
> on basement membranes. The advantage here is not so much the fluorescence,
> but that we use unfixed frozen sections. The buffer rinse before antibody
> application, removes un-bound serum immunoglobulins, leaving any
> pathological bound Igs for the IF antibody to bind to. This gives a clean
> result.
>
> If one would do IF on formalin-fixed paraffin sections of renal or skin
> biopsies, you would find heavy background due to the fixative cross-linking
> serum Igs to tissue and cells (which would usually be removed by the buffer
> rinse if unfixed frozen sections were used - see above).
>
> IF, apart from being a historic method, also does not suffer from
> endogenous peroxidase that would need to be blocked if peroxidase was used
> in place of fluorescence.
>
>
>
> Regards
> Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
> Principal Scientist, the Children's Hospital at Westmead
> Adjunct Fellow, School of Medicine, University of Western Sydney
> Tel: 612 9845 3306
> Fax: 612 9845 3318
> Pathology Department
> the children's hospital at westmead
> Cnr Hawkesbury Road and Hainsworth Street, Westmead
> Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
>
> -Original Message-
> From: Blanca Lopez via Histonet [mailto:histonet@lists.utsouthwestern.edu]
> Sent: Thursday, 13 April 2017 11:10 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] help!!
>
> Hello!
> I just need a help with a simple question...Is anyone can explain me what
> is the purpose between performing immunohistochemistry and
> Immunofluorescence?
> Thanks  :)
>
> Blanca Lopez
> Histotech (ASCP)
> UTSW Tissue Resource K1.210
> Simmons Comprehensive Cancer Center
> UT Southwestern Medical Center
> Telephone: 214-648-7598
> Email: blanca.lo...@utsouthwestern.edu
>
>
> 
>
> UT Southwestern
>
>
> Medical Center
>
>
>
> The future of medicine, today.
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> This message is intended for the addressee named and may contain
> confidential information. If you are not the intended recipient, please
> delete it and notify the sender.
>
> Views expressed in this message are those of the individual sender, and
> are not necessarily the views of NSW Health or any of its entities.
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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