[Histonet] Looking for used microtome

[Jill Cox via Histonet]

Hi all,
I'm looking for a good used microtome preferably Sakura SRM 200 or newer Leica. 
Hoping to purchase local near Phoenix AZ. It's for a lab I help out part time 
here. Found a couple on eBay but just want to make sure it is in good working 
condition. Thank you!!!

Jill Cox HT ASCP

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Re: [Histonet] Help with preparing slides of baby guppies

[J B via Histonet]

Jenn,

Trying to fully understand. You need someone to prepare these slides again
for you?  Great quality work, you provide the specimen?  Let me know, I
would love to learn more.

Sincerely,

JB

On Wed, May 31, 2017, 9:13 AM Dearolf, Jenn via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello!
>
> My name is Jenn Dearolf, and I am a professor in the Biology Department at
> Hendrix College, a small liberal arts college in Conway, AR.  I have
> written this list before to get advice about how to prevent freezing
> artifact in small muscle samples (Thanks!), but today, I have a completely
> different need.
>
> In our Zoology course at Hendrix, we use slides that have numerous serial
> sections (7 to 10 microns thickness) of a baby guppy on them that have been
> stained with H & E.  And, a box of slides, ranging from 8 to 12 slides, is
> the entire guppy sectioned from the tip of its nose to the tip of its
> tail.  However, these slides are very old, and over the years, the mounting
> media has pulled away from the sections.  In addition, numerous slides have
> been broken or lost.
>
> Unfortunately, no one in my Department now has the necessary skills to
> produce these slides for our students.  I was wondering if anyone on the
> list knew of a facility that could produce these slides.  I think we should
> be able to provide the baby guppies.  I would just have to get approval
> from our IACUC.
>
> I also have a very old paper that discusses the steps that were necessary
> to produce the slides.  I am happy to share the methodology with anyone
> that could help us, and we could discuss what we would need to do at
> Hendrix and which steps would need to be performed at your facility.
>
> I appreciate any advice folks are willing to share.  Thanks for your time
> and consideration.
>
> Sincerely,
> Jenn
>
>
> Jennifer Dearolf, Ph.D.
> Professor and Chair
> Biology Department
> Hendrix College
> 1600 Washington Ave.
> Conway, AR 72032
> (501) 450-4530 (office)
>
>
>
>
>
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>
-- 

Have a great day!
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[Histonet] Help with preparing slides of baby guppies

[Dearolf, Jenn via Histonet]

Hello!

My name is Jenn Dearolf, and I am a professor in the Biology Department at 
Hendrix College, a small liberal arts college in Conway, AR.  I have written 
this list before to get advice about how to prevent freezing artifact in small 
muscle samples (Thanks!), but today, I have a completely different need.

In our Zoology course at Hendrix, we use slides that have numerous serial 
sections (7 to 10 microns thickness) of a baby guppy on them that have been 
stained with H & E.  And, a box of slides, ranging from 8 to 12 slides, is the 
entire guppy sectioned from the tip of its nose to the tip of its tail.  
However, these slides are very old, and over the years, the mounting media has 
pulled away from the sections.  In addition, numerous slides have been broken 
or lost.

Unfortunately, no one in my Department now has the necessary skills to produce 
these slides for our students.  I was wondering if anyone on the list knew of a 
facility that could produce these slides.  I think we should be able to provide 
the baby guppies.  I would just have to get approval from our IACUC.

I also have a very old paper that discusses the steps that were necessary to 
produce the slides.  I am happy to share the methodology with anyone that could 
help us, and we could discuss what we would need to do at Hendrix and which 
steps would need to be performed at your facility.

I appreciate any advice folks are willing to share.  Thanks for your time and 
consideration.

Sincerely,
Jenn


Jennifer Dearolf, Ph.D.
Professor and Chair
Biology Department
Hendrix College
1600 Washington Ave.
Conway, AR 72032
(501) 450-4530 (office)





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Re: [Histonet] Histonet Digest, Vol 162, Issue 26 butterflies

[Steve McClain via Histonet]

I have no experiences w butterflies, but the chitin in ticks, carpenter ants 
and spiders does not cut well.  
For insects and arthropods we post-fix in Carnoy's for 60 minutes
Then soften with 10% KOH RT for 10-20 minutes 
then wash for 30-60minutes in water. Long processing cycle. 

Steve A. McClain, MD

On May 30, 2017, at 13:26, "histonet-requ...@lists.utsouthwestern.edu" 
 wrote:

>> Does anyone have any experience processing and paraffin embedding
>> butterflies?
>> 
>> Rhonda Gregoire, MLT
>> Supervisor, Clinical Pathology
>> Veterinary Diagnostic Services
>> Manitoba Agriculture
>> 545 University Crescent, Winnipeg, MB, R3T 5S6
>> rhonda.grego...@gov.mb.ca
>> T : 204-945-7641  F : 204-945-7646

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