Re: [Histonet] Elastic Stain

2017-11-08 Thread Caroline Miller via Histonet 
In the UK clinical lab I started in we used Miller's elastin, which I think
was a resourcinol formulation (digging from 20 years ago in my brain). I
tried to find that in the US but never did. I loved that stain, worked well
every time! We would counterstain with a van-Giesen, looked stunning! the
elastin came out very dark blue / black.

mills

On Wed, Nov 8, 2017 at 6:31 AM, Terri Braud via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> We love the Aldehyde Fuchsin with a fast-green counterstain.  Once the
> initial working stain is made up, it is a fast and easy stain to perform,
> not to mention just pretty - purple fibers against a green background.
>
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Laboratory
> Holy Redeemer Hospital
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> ph: 215-938-3689
> fax: 215-938-3874
> Care, Comfort, and Heal
> *
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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>



-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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Re: [Histonet] Metal embedding molds-large

2017-11-08 Thread Caroline Miller via Histonet 
hey, we do all our embedding in those molds, and here is what I suggest:

1 - Make sure to have enough wax in the back of the molds, all the way
until it is on the lip of the cassette - you may need to refill a  bunch of
times because the wax drains out (why regular sakura cassettes do not fit
in these molds, also made by sakura I really don't know). But you need to
wait until it hardens enough, but not too much to leave a transition
between the two fill waxes. This will prevent the cassette from coming away
when you pop it out
2 - Make sure the block is nice and cold, wait 10 minutes longer than you
think you have to
3 - Use a spatula or other strong item to place under the label side of the
cassette and pop out of the mold, if you get any resistance then WAIT some
more! (again them being cold is really important)

good luck, happy to answer any clarifying questions!

mills

On Wed, Nov 8, 2017 at 12:30 PM, Bryan Llewellyn via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Sorry!
>
> That should be TSP - trisodium phosphate - not TCP, which might make it
> worse.
>
> Bryan
>
>
>
> Bryan Llewellyn wrote:
>
>> This used to be a common problem years ago. It is due to crud buildup on
>> the metal. Boil them with TCP for half an hour, then thoroughly wash
>> them in cold water. Coat them with a VERY light smear of glycerol before
>> you use them, preferably each time. That should help.
>>
>> Bryan Llewellyn.
>>
>> Diane Satterfield via Histonet wrote:
>>
>>> We are using large metal molds to embed mouse brains.  We are having a
>>> hard time getting to block out of the molds, the paraffin blocks are
>>> sticking.  Sometimes they are coming out cracked.  Sometimes the
>>> cassette comes off the paraffin block.  Any idea why this is
>>> happening? Any advice on how to fix this problem?
>>>
>>>
>>> Diane L. Satterfield, BS
>>> Manager Brain Tumor BioRepository
>>> Research Program Leader
>>> Duke University Medical Center
>>> Brain Tumor Center Biorepository and Database
>>>
>>> diane.satterfi...@duke.edu
>>> office  919-684-4642
>>> pager  919-970-7328
>>> fax  919-684-4975
>>>
>>> CONFIDENTIALITY NOTICE:  The information contained in this electronic
>>> mail is sensitive, protected information intended only for the
>>> addressee(s).  Any other person, including anyone who believes he/she
>>> might have received it due to an addressing error, is requested to
>>> notify the sender immediately by return electronic mail, and to delete
>>> it without further reading or retention.  The information is not to be
>>> forwarded to or shared unless in compliance with Duke Medicine
>>> policies on confidentiality and/or with the approval of the sender.
>>>
>>>
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>>>
>>
>
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-- 
Caroline Miller (mills)
Director of Histology
3Scan.com
415 2187297
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Re: [Histonet] Metal embedding molds-large

2017-11-08 Thread Bryan Llewellyn via Histonet 

Sorry!

That should be TSP - trisodium phosphate - not TCP, which might make it 
worse.


Bryan


Bryan Llewellyn wrote:

This used to be a common problem years ago. It is due to crud buildup on
the metal. Boil them with TCP for half an hour, then thoroughly wash
them in cold water. Coat them with a VERY light smear of glycerol before
you use them, preferably each time. That should help.

Bryan Llewellyn.

Diane Satterfield via Histonet wrote:

We are using large metal molds to embed mouse brains.  We are having a
hard time getting to block out of the molds, the paraffin blocks are
sticking.  Sometimes they are coming out cracked.  Sometimes the
cassette comes off the paraffin block.  Any idea why this is
happening? Any advice on how to fix this problem?


Diane L. Satterfield, BS
Manager Brain Tumor BioRepository
Research Program Leader
Duke University Medical Center
Brain Tumor Center Biorepository and Database

diane.satterfi...@duke.edu
office  919-684-4642
pager  919-970-7328
fax  919-684-4975

CONFIDENTIALITY NOTICE:  The information contained in this electronic
mail is sensitive, protected information intended only for the
addressee(s).  Any other person, including anyone who believes he/she
might have received it due to an addressing error, is requested to
notify the sender immediately by return electronic mail, and to delete
it without further reading or retention.  The information is not to be
forwarded to or shared unless in compliance with Duke Medicine
policies on confidentiality and/or with the approval of the sender.


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Re: [Histonet] Metal embedding molds-large

2017-11-08 Thread Bryan Llewellyn via Histonet 
This used to be a common problem years ago. It is due to crud buildup on 
the metal. Boil them with TCP for half an hour, then thoroughly wash 
them in cold water. Coat them with a VERY light smear of glycerol before 
you use them, preferably each time. That should help.


Bryan Llewellyn.

Diane Satterfield via Histonet wrote:

We are using large metal molds to embed mouse brains.  We are having a hard 
time getting to block out of the molds, the paraffin blocks are sticking.  
Sometimes they are coming out cracked.  Sometimes the cassette comes off the 
paraffin block.  Any idea why this is happening? Any advice on how to fix this 
problem?


Diane L. Satterfield, BS
Manager Brain Tumor BioRepository
Research Program Leader
Duke University Medical Center
Brain Tumor Center Biorepository and Database

diane.satterfi...@duke.edu
office  919-684-4642
pager  919-970-7328
fax  919-684-4975

CONFIDENTIALITY NOTICE:  The information contained in this electronic mail is 
sensitive, protected information intended only for the addressee(s).  Any other 
person, including anyone who believes he/she might have received it due to an 
addressing error, is requested to notify the sender immediately by return 
electronic mail, and to delete it without further reading or retention.  The 
information is not to be forwarded to or shared unless in compliance with Duke 
Medicine policies on confidentiality and/or with the approval of the sender.


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Re: [Histonet] Metal embedding molds-large

2017-11-08 Thread Jay Lundgren via Histonet 
mold release


Virus-free.
www.avg.com

<#DAB4FAD8-2DD7-40BB-A1B8-4E2AA1F9FDF2>

On Wed, Nov 8, 2017 at 11:42 AM, Diane Satterfield via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> We are using large metal molds to embed mouse brains.  We are having a
> hard time getting to block out of the molds, the paraffin blocks are
> sticking.  Sometimes they are coming out cracked.  Sometimes the cassette
> comes off the paraffin block.  Any idea why this is happening? Any advice
> on how to fix this problem?
>
>
> Diane L. Satterfield, BS
> Manager Brain Tumor BioRepository
> Research Program Leader
> Duke University Medical Center
> Brain Tumor Center Biorepository and Database
>
> diane.satterfi...@duke.edu
> office  919-684-4642
> pager  919-970-7328
> fax  919-684-4975
>
> CONFIDENTIALITY NOTICE:  The information contained in this electronic mail
> is sensitive, protected information intended only for the addressee(s).
> Any other person, including anyone who believes he/she might have received
> it due to an addressing error, is requested to notify the sender
> immediately by return electronic mail, and to delete it without further
> reading or retention.  The information is not to be forwarded to or shared
> unless in compliance with Duke Medicine policies on confidentiality and/or
> with the approval of the sender.
>
>
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
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[Histonet] Metal embedding molds-large

2017-11-08 Thread Diane Satterfield via Histonet 
We are using large metal molds to embed mouse brains.  We are having a hard 
time getting to block out of the molds, the paraffin blocks are sticking.  
Sometimes they are coming out cracked.  Sometimes the cassette comes off the 
paraffin block.  Any idea why this is happening? Any advice on how to fix this 
problem?


Diane L. Satterfield, BS
Manager Brain Tumor BioRepository
Research Program Leader
Duke University Medical Center
Brain Tumor Center Biorepository and Database

diane.satterfi...@duke.edu
office  919-684-4642
pager  919-970-7328
fax  919-684-4975

CONFIDENTIALITY NOTICE:  The information contained in this electronic mail is 
sensitive, protected information intended only for the addressee(s).  Any other 
person, including anyone who believes he/she might have received it due to an 
addressing error, is requested to notify the sender immediately by return 
electronic mail, and to delete it without further reading or retention.  The 
information is not to be forwarded to or shared unless in compliance with Duke 
Medicine policies on confidentiality and/or with the approval of the sender.


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[Histonet] NK Cell markers for Mouse in Paraffin

2017-11-08 Thread Amy Porter via Histonet 
Hi all - anyone out there have an antibody that they like for NK Cells in a
mouse model - I am trying to work with a mouse monoclonal (MOM) with polymer
technology.  Looking for assistance from labs that have established
protocols.  We have tried enzymatic and heat retrieval ..not getting good
results.  Any comments would be appreciated - willing to change antibody
vendors 

currently using clone PK136.  Thanks - Amy

 

Amy S. Porter, HT (ASCP) 

Michigan State University - Department of Physiology

Investigative HistoPathology Lab - Supervisor 

Research Core Support Facility

567 Wilson Road - Room 2201

East Lansing, MI  48824-6458

517-884-5026

port...@msu.edu

 

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[Histonet] mounting medium

2017-11-08 Thread warda hassan via Histonet 
Hello to all

Can anyone suggest which mouting medium is best that will help preservation
of staining properties without creating fading of stains and bubbles on
long run.
Thanks alot
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Re: [Histonet] Division of Path cases

2017-11-08 Thread Terri Braud via Histonet 
We give the worklist to the chief Pathologist and let him make the assignments 
on a daily basis.  They are usually divided up by complexity. In the absence of 
the Chief Path, then I make the assignments.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal

*


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Re: [Histonet] Elastic Stain

2017-11-08 Thread Terri Braud via Histonet 
We love the Aldehyde Fuchsin with a fast-green counterstain.  Once the initial 
working stain is made up, it is a fast and easy stain to perform, not to 
mention just pretty - purple fibers against a green background.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Laboratory
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
ph: 215-938-3689
fax: 215-938-3874
Care, Comfort, and Heal
*


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