Re: [Histonet] Mouse bladder

2017-12-19 Thread Colleen Forster via Histonet 
Exactly...and then it wont shrivel up

C

On Tue, Dec 19, 2017 at 3:09 PM, dusko trajkovic 
wrote:

> I agree with Colleen, processing is the issue. You need to process on a
> longer program in order to dehydrate and clear the agar properly.
> dusko
>
>
> On Tuesday, December 19, 2017 9:45 AM, Colleen Forster via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
>
> Be sure you are processing them on an overnight process. Even if the
> samples are small it takes that time to process the agar properly.
>
> I use Histogel and learned this lesson the hard way. My samples always
> "shriveled" up too until someone enlightened me about the processing time.
> I would think agar might be the same.
>
>
> Just a thought,
>
> Colleen Forster HT(ASCP)QIHC
> U of MN
>
> On Tue, Dec 19, 2017 at 11:29 AM, Liz Chlipala via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
> > I would try histogel instead of agar.
> >
> > Liz
> >
> > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> > Premier Laboratory, LLC
> > PO Box 18592
> > Boulder, CO 80308
> > (303) 682-3949 office
> > (303) 682-9060 fax
> > (303) 881-0763 cell
> > l...@premierlab.com
> > www.premierlab.com
> >
> > Ship to Address:
> >
> > Premier Laboratory, LLC
> > 1567 Skyway Drive, Unit E
> 
> > Longmont, CO 80504
> 
> >
> > From: Heather Marlatt via Histonet [mailto:histonet@lists.
> > utsouthwestern.edu]
> > Sent: Tuesday, December 19, 2017 9:12 AM
> > To: histonet@lists.utsouthwestern.edu
> > Subject: [Histonet] Mouse bladder
> >
> > Does anyone have a good protocol for mouse bladder embedded in agar that
> > they are willing to share? We have mouse bladders in 2% agar and have
> tried
> > a few different protocol variations from our usual mouse but the agar
> keeps
> > shriveling up.
> >
> > Thanks
> > Heather
> > ___
> > Histonet mailing list
> > Histonet@lists.utsouthwestern.edu utsouthwestern.edu
> > >
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> >
> > 
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>
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Re: [Histonet] Mouse bladder

2017-12-19 Thread dusko trajkovic via Histonet 
I agree with Colleen, processing is the issue. You need to process on a longer 
program in order to dehydrate and clear the agar properly.dusko 

On Tuesday, December 19, 2017 9:45 AM, Colleen Forster via Histonet 
 wrote:
 

 Be sure you are processing them on an overnight process. Even if the
samples are small it takes that time to process the agar properly.

I use Histogel and learned this lesson the hard way. My samples always
"shriveled" up too until someone enlightened me about the processing time.
I would think agar might be the same.


Just a thought,

Colleen Forster HT(ASCP)QIHC
U of MN

On Tue, Dec 19, 2017 at 11:29 AM, Liz Chlipala via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> I would try histogel instead of agar.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> l...@premierlab.com
> www.premierlab.com
>
> Ship to Address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
>
> From: Heather Marlatt via Histonet [mailto:histonet@lists.
> utsouthwestern.edu]
> Sent: Tuesday, December 19, 2017 9:12 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Mouse bladder
>
> Does anyone have a good protocol for mouse bladder embedded in agar that
> they are willing to share? We have mouse bladders in 2% agar and have tried
> a few different protocol variations from our usual mouse but the agar keeps
> shriveling up.
>
> Thanks
> Heather
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu >
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> 
> ___
> Histonet mailing list
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> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
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[Histonet] tissue blocks

2017-12-19 Thread Mica Fulgham via Histonet 
Does anyone have any breast tissue or skin FFPE blocks that would be willing to 
send me?  5 blocks of each would be greatly appreciated.
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Re: [Histonet] Mouse bladder

2017-12-19 Thread Colleen Forster via Histonet 
Be sure you are processing them on an overnight process. Even if the
samples are small it takes that time to process the agar properly.

I use Histogel and learned this lesson the hard way. My samples always
"shriveled" up too until someone enlightened me about the processing time.
I would think agar might be the same.


Just a thought,

Colleen Forster HT(ASCP)QIHC
U of MN

On Tue, Dec 19, 2017 at 11:29 AM, Liz Chlipala via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> I would try histogel instead of agar.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> l...@premierlab.com
> www.premierlab.com
>
> Ship to Address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
>
> From: Heather Marlatt via Histonet [mailto:histonet@lists.
> utsouthwestern.edu]
> Sent: Tuesday, December 19, 2017 9:12 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Mouse bladder
>
> Does anyone have a good protocol for mouse bladder embedded in agar that
> they are willing to share? We have mouse bladders in 2% agar and have tried
> a few different protocol variations from our usual mouse but the agar keeps
> shriveling up.
>
> Thanks
> Heather
> ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu >
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> 
> ___
> Histonet mailing list
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Re: [Histonet] Mouse bladder

2017-12-19 Thread Liz Chlipala via Histonet 
I would try histogel instead of agar.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

From: Heather Marlatt via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, December 19, 2017 9:12 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mouse bladder

Does anyone have a good protocol for mouse bladder embedded in agar that
they are willing to share? We have mouse bladders in 2% agar and have tried
a few different protocol variations from our usual mouse but the agar keeps
shriveling up.

Thanks
Heather
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[Histonet] Mouse bladder

2017-12-19 Thread Heather Marlatt via Histonet 
Does anyone have a good protocol for mouse bladder embedded in agar that
they are willing to share? We have mouse bladders in 2% agar and have tried
a few different protocol variations from our usual mouse but the agar keeps
shriveling up.

Thanks
Heather
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[Histonet] PCR sectioning

2017-12-19 Thread Sanders, Jeanine (CDC/OID/NCEZID) via Histonet 
Good morning!

Do any of you out there section for PCR? If so, do you have a preference in 
microtomes?

Thanks!

Jeanine H. Sanders
Infectious Diseases Pathology Branch
Centers for Disease Control and Prevention
1600 Clifton Rd., NE MS-G32
Atlanta, GA 30329


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