Re: [Histonet] Acid Phosphatase Protocol

[Tony Henwood (SCHN) via Histonet]

Hi April,
Try this

Control tissues: liver, kidney and prostate
Fixation and Sectioning: Air dried unfixed 8µm frozen sections

Solutions:

1.  Substrate solution
Naphtol AS-B1 phosphate 0.005g (Fluka 70494  1g)
Dimethylfomamide0.5ml
Warning: Suspected Carcinogen – see MSDS

2.  Acetate buffer pH 4.8
Sodium acetate  4.8g 
Distilled water 100ml
0.6% Acetic Acid14.75ml
pH to 4.8
Make up to 1000 ml with distilled water

3.  Sodium nitrite: 
Warning: Toxic – see MSDS
Sodium nitrite  1.0g
Distilled water 25.0ml

4.  Pararosaniline-HCl stock solution. 
Warning: Suspected Carcinogen – see MSDS
Pararosaniline hydrochloride 
(Basic Fuchsin CI 42510)0.8g
Distilled water 16ml
Concentrated Hydrochloric acid  4ml
Heat gently, cool to room temperature and filter (store 4C)

5.  Incubation solution:
Take 0.4ml solution 3 + 0.4ml solution 4 and mix. Stand for 2 minutes and then 
add to:
Substrate Solution (Solution 1) 0.5ml
Acetate buffer pH 4.8   25ml
Adjust with 0.1M NaOH to pH 4.5-5.0.

Procedure:

1.  Take air-dried sections and incubate in the incubation solution at 37oC 
for 1 hour
2.  Wash with distilled water and counterstain with Harris's Haematoxylin 
for 1 minute.
3.  Wash in running tap water
4.  Dehydrate rapidly through fresh alcohols to xylene and mount.

Results:

Acid phosphatase activity   red
Nuclei  blue

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA


From: Oler, April via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Friday, January 26, 2018 6:26 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Acid Phosphatase Protocol

Good afternoon,

I am writing to you all because I am in search of Acid Phosphatase muscle 
protocols that do not utilize sodium barbital. I'm hoping to eliminating sodium 
barbital from my lab in the near future. If anyone is willing to share their 
protocol, or advice, I would very much appreciate any assistance.

Thank you!

April Oler, HT(ASCP)
Senior Clinical Technologist
IPOX/DIF/Muscle Laboratories
Michigan Medicine
Ann Arbor, MI




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Re: [Histonet] Image analysis on breast markers

[Liz Chlipala via Histonet]

Teresa

CAP should be releasing a paper soon, within the next couple months.  
Quantitative Image Analysis (QIA) for HER2 Immunohistochemistry for Breast 
Cancer, it will address the question that  you have.  Keep any eye out for it.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
l...@premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

From: Lima, Teresa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Thursday, January 25, 2018 12:48 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Image analysis on breast markers

Hello all,
I am wondering if any of you out there that do image analysis of breast markers 
have a technologist select the field of view to be analyzed by the system. Our 
techs do this with no supervision by a pathologist. In many instances the 
pathologist changes the analyzed result without logging in to the system to 
check which areas were selected by the tech. This makes me just a bit nervous 
and I will be interested to see what you all are doing. Thanks and I look 
forward to your replies.

Many thanks,
Terri

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[Histonet] Image analysis on breast markers

[Lima, Teresa via Histonet]

Hello all,
I am wondering if any of you out there that do image analysis of breast markers 
have a technologist select the field of view to be analyzed by the system. Our 
techs do this with no supervision by a pathologist. In many instances the 
pathologist changes the analyzed result without logging in to the system to 
check which areas were selected by the tech. This makes me just a bit nervous 
and I will be interested to see what you all are doing. Thanks and I look 
forward to your replies.

Many thanks,
Terri

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confidential and exempt from disclosure under applicable law. If you are not 
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Re: [Histonet] Histotechnician open positions

[Willis, Donna G. via Histonet]

Good Afternoon Histology world,
Baylor Scott and White, Baylor University Medical Center Dallas has 2 open 
position on the evening/night shift, in the routine Histology laboratory.  1 
position is full time from 6pm to 2:30am and the other is a part time position 
from 2am to 6am.  Application to these position can be placed on the BSWHealth 
website.

Donna Willis, HT/HTL(ASCP)
Anatomic Pathology Manager
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas  75246
214-820-2465 office|214-725-6184 mobile
BaylorScottandWhite.com

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and Baylor Scott & White Health, and its affiliated entities, hereby expressly 
reserve all privileges and confidentiality that might otherwise be waived as a 
result of an erroneous or misdirected e-mail transmission. No employee or agent 
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[Histonet] Leica Reichert-Jung cryocut 1800 cryostat manual

[Alex Horkowitz via Histonet]

Hello All,

I am in need of a manual for a Leica Reichert-Jung cryocut 1800 cryostat with 
2020 microtome.  Any tips for sectioning mouse lung with this system would be 
much appreciated as well.

Cheers,

Alex Horkowitz
San Diego State University
2nd Year Cell & Molecular Biology MS Student
alexhorkow...@mac.com


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[Histonet] Acid Phosphatase Protocol

[Oler, April via Histonet]

Good afternoon,

I am writing to you all because I am in search of Acid Phosphatase muscle 
protocols that do not utilize sodium barbital. I'm hoping to eliminating sodium 
barbital from my lab in the near future. If anyone is willing to share their 
protocol, or advice, I would very much appreciate any assistance.

Thank you!

April Oler, HT(ASCP)
Senior Clinical Technologist
IPOX/DIF/Muscle Laboratories
Michigan Medicine
Ann Arbor, MI




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[Histonet] tracking slide filing

[Minikus, Carrie A. via Histonet]

We would like to get an automated tracking system sometime in the future; 
however, right now it is not in our budget. We currently have no tracking 
system for who is filing our slides away into the file drawers and we have been 
having some real issues with slides not being able to be found due to incorrect 
filing of the slides. Huge Problem! Other than automated tracking systems, what 
are some other ways your facilities use a tracking system to know who is filing 
your slides or who might have last handled the slides? Thanks for any and all 
suggestions!



Carrie Minikus, MLS(ASCP)CM, HTL(ASCP)CM

Histology Manager



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[Histonet] Making Bulk Reagents for Ventana

[Nutter, James E via Histonet]

Hi Everyone,

Has anyone or does anyone make the bulk reagents for the Ventana Ultras?  (LCS, 
SSC, Reaction Buffer, CC1, CC2)

If so:
How to turn off the tracking system for bulk reagents?
Formulations?
Success?

Thank you all,

James Nutter
Chantilly, VA

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[Histonet] Used equipment

[Campbell, Tasha M. via Histonet]

Anyone know who to contact to sell used equipment to? Thanks!!




Tasha Campbell, B.S.,HTL(ASCP)
Frederick Gastroenterology Associates
310 W. 9th St.
Frederick, MD 21701
301-695-6800 ext. 144

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